Abstract：
:Binding of epidermal growth factor (EGF) to its receptor was evaluated in the four cultured choriocarcinoma cell lines BeWo, NaUCC-1, NaUCC-2, and NaUCC-3. Also, the effect of the anti-tumor drugs actinomycin-D (Act-D) and methotrexate (MTX) on the EGF receptor binding was investigated in these cell lines. Incubation of these cells with [125I]EGF at 37 degrees C resulted in a higher binding than that at 22 degrees C or at 4 degrees C. These bindings were saturable during 30- to 60-min incubation, and were specific and reversible. Scatchard analysis showed that the maximal number of receptor binding sites was 2.89 X 10(3)/cell in BeWo cells, 2.04 X 10(3)/cell in NaUCC-1 cells, 1.84 X 10(3)/cell in NaUCC-2 cells, and 1.01 X 10(3)/cell in NaUCC-3 cells. Preincubation with Act-D or MTX for 24 hr decreased the number of receptor binding sites (26%-53%) and slightly increased the receptor binding affinities. Combination of the two drugs resulted in a further diminution of EGF receptor binding sites in BeWo, NaUCC-1, and NaUCC-3 cells, respectively, but reversed the Act-D effect in NaUCC-2 cells. These results indicated that choriocarcinoma tissue is rich in EGF receptors, that the anti-tumor drugs Act-D and MTX diminish the receptor binding sites in the tissue, and suggest that MTX might induce a drug resistance to Act-D in some choriocarcinoma tissue.
journal_name
Nagoya J Med Scijournal_title
Nagoya journal of medical scienceauthors
Chen F,Goto S,Nawa A,Okamoto T,Tomoda Ysubject
Has Abstractpub_date
1990-03-01 00:00:00pages
5-11issue
1-4eissn
0027-7622issn
2186-3326journal_volume
52pub_type
杂志文章abstract：:The number of pollinosis patients in Japan has significantly increased over the past 20 years. The majority of genome-wide association studies (GWAS) on pollinosis have been conducted in subjects of European descent, with few studies in Japanese populations. The aim of our GWAS was to identify genetic loci associated ...
journal_title：Nagoya journal of medical science
pub_type： 杂志文章
doi：10.18999/nagjms.80.1.109
更新日期：2018-02-01 00:00:00
abstract：:Autologous nerve transplantation has been the gold standard in the treatment of facial nerve injury, however it has not been achieved satisfactory result and needs donor sacrifice. A polyglycolic acid collagen conduit (Nerbridge, Toyobo Co., Japan) has the potential to compare to or exceed autologous nerve grafts in p...
journal_title：Nagoya journal of medical science
pub_type：
doi：10.18999/nagjms.82.1.123
更新日期：2020-02-01 00:00:00
abstract：:We report a patient with a multicystic kidney treated successfully by continuous percutaneous drainage. This method may be an alternative to surgical resection of a multicystic kidney that has not involved after one year of follow-up. ...
journal_title：Nagoya journal of medical science
pub_type： 杂志文章
doi：
更新日期：1993-11-01 00:00:00
abstract：:Umbilical metastasis from intra-abdominal or pelvic malignancy, which is called Sister Mary Joseph's nodule (SMJN), is rare, and it has a poor prognosis. Its most common primary sites are the stomach and ovaries. SMJN caused by colon cancer is uncommon. A 42-year-old woman visited local clinics with complaints of an u...
journal_title：Nagoya journal of medical science
pub_type：
doi：10.18999/nagjms.81.2.325
更新日期：2019-05-01 00:00:00
abstract：:Hyperperfusion syndrome occurs after treatment of a large or giant cerebral aneurysm. Recently, flow-diverter stent placement has emerged as an effective treatment method for a large cerebral aneurysm, but postoperative ipsilateral delayed intraparenchymal hemorrhage occurs in a minority of cases. The mechanism underl...
journal_title：Nagoya journal of medical science
pub_type： 杂志文章
doi：10.18999/nagjms.81.4.629
更新日期：2019-11-01 00:00:00
abstract：:The effects of intravenous administration of iloprost, a prostacyclin analogue, on myocardial energy metabolism and myocardial blood flow (MBF) were examined in anesthetized open-chest dogs subjected to 60 min of myocardial ischemia by coronary ligation. Iloprost administration at levels of 0.1 or 0.2 micrograms/kg/mi...
journal_title：Nagoya journal of medical science
pub_type： 杂志文章
doi：
更新日期：1994-03-01 00:00:00
abstract：:Anastomotic leakage after esophagectomy is associated with prolonged hospitalization and increased medical cost. Additionally, it sometimes leads to a fatal condition and impaired postoperative quality of life. During the process of wound healing, β-hydroxy-β-methylbutyrate (HMB) is important for collagen biosynthesis...
journal_title：Nagoya journal of medical science
pub_type： 杂志文章
doi：10.18999/nagjms.82.1.33
更新日期：2020-02-01 00:00:00
abstract：:To evaluate the circulatory disturbances, sensory disturbances and damage to muscles and joints by chain saw vibration exposure, the process of the deterioration of the symptoms with chain saw operating time was studied. Subjects were classified into eight groups according to TOT (Total Operating Time): Group 0, 46 co...
journal_title：Nagoya journal of medical science
pub_type： 杂志文章
doi：
更新日期：1994-05-01 00:00:00
abstract：:Vitamin K deficiency bleeding (VKDB) is a preventable cause of infant mortality and long-term morbidity through the world. This study aimed to demonstrate the costs of VKDB treatment estimated from the hospital records in Tashkent, Uzbekistan, as well as the prophylaxis costs for mass vitamin K medication. Subjects we...
journal_title：Nagoya journal of medical science
pub_type： 杂志文章
doi：10.18999/nagjms.80.1.11
更新日期：2018-02-01 00:00:00
abstract：:Thoracoscopic lobectomy has recently become a widely used surgical treatment for congenital cystic lung disease, but significant issues can arise in some cases, such as a limited working space in neonates, a limited view in cases involving large cystic lesions. We reviewed the treatment outcomes of neonates that under...
journal_title：Nagoya journal of medical science
pub_type： 杂志文章
doi：10.18999/nagjms.78.4.447
更新日期：2016-12-01 00:00:00
abstract：:Being homebound has been reported to be associated with a number of conditions. In the current study, the incidence of homebound individuals was surveyed in an urban city area in Japan. The city office randomly enrolled 5,000 residents of Nagoya City aged 65 and over. A questionnaire was sent to their principal caregi...
journal_title：Nagoya journal of medical science
pub_type： 杂志文章
doi：
更新日期：2015-08-01 00:00:00
abstract：:Metabolic syndrome (MetS) is characterized by the presence of atherogenic risk factors, and is associated with a marked increase in the risk of cardiovascular disease. Recently, the criteria of MetS were newly defined in Japan. We examined the relationship between MetS and the various metabolic parameters in Japanese ...
journal_title：Nagoya journal of medical science
pub_type： 杂志文章
doi：
更新日期：2008-03-01 00:00:00
abstract：:Experimental middle ear barotrauma was studied morphologically. White guinea pigs were placed in an experimental hyperbaric chamber, and middle ear barotrauma was created by increasing the pressure in the hyperbaric chamber from 1 atmosphere absolute (ATA) to 2 ATA using pure oxygen, maintaining the pressure at 2 ATA ...
journal_title：Nagoya journal of medical science
pub_type： 杂志文章,评审
doi：
更新日期：1997-11-01 00:00:00
abstract：:Yokukansankachimpihange (YKSCH), a traditional Japanese medicine, is widely used for the amelioration of the behavioral and psychological symptoms of dementia with digestive dysfunction. Regardless of its successful use for digestive dysfunction, the effect of YKSCH on body weight was unknown. Furthermore, if YKSCH in...
journal_title：Nagoya journal of medical science
pub_type： 杂志文章
doi：10.18999/nagjms.79.3.351
更新日期：2017-08-01 00:00:00
abstract：:The cutoff values of fractional flow reserve (FFR) to detect physiological myocardial ischemia are still controversial. Some studies have reported that left ventricular (LV) dyssynchrony occurs in patients with coronary artery disease (CAD). The purpose of this study was to investigate LV dyssynchrony in patients with...
journal_title：Nagoya journal of medical science
pub_type： 杂志文章
doi：
更新日期：2015-02-01 00:00:00
abstract：:We attempted to clarify the storage iron metabolism from the change in the serum ferritin level. We assumed that the nonlinear decrease in serum ferritin was caused by serum ferritin increase in iron mobilization. Under this assumption, we determined both ferritin and hemosiderin iron levels by computer-assisted simul...
journal_title：Nagoya journal of medical science
pub_type： 杂志文章
doi：
更新日期：2012-02-01 00:00:00
abstract：:Stereotactic radiosurgery for large brain metastases (BM) not amenable to surgical resection is associated with limited local control and neurotoxicity, while hypofractionated stereotactic radiotherapy (HFSRT) has emerged as a promising option. We retrospectively evaluated 61 patients with BM larger than 2 cm in the m...
journal_title：Nagoya journal of medical science
pub_type： 杂志文章
doi：10.18999/nagjms.81.3.397
更新日期：2019-08-01 00:00:00
abstract：:The aim of this study was to identify the socio-demographic characteristics, knowledge, attitude and accessibility factors related to the utilization of antenatal care (ANC) service among pregnant women in the Kham District, Laos. Data for this cross-sectional study were collected in July 2008, using a two-stage clust...
journal_title：Nagoya journal of medical science
pub_type： 杂志文章
doi：
更新日期：2010-02-01 00:00:00
abstract：:The Japanese Orthopaedic Association has proposed the term "locomotive syndrome" to designate a condition that places a person at high risk for long-term care. However, in daily clinical practice, even when a diagnosis of locomotive syndrome is made, exercise therapy often cannot be successfully performed in some pati...
journal_title：Nagoya journal of medical science
pub_type： 杂志文章
doi：10.18999/nagjms.79.1.43
更新日期：2017-02-01 00:00:00
abstract：:Liver biopsy specimens from 18 patients with primary biliary cirrhosis were examined histochemically and by energy-dispersive x-ray microanalysis. Using two indices, we classified hepatic copper accumulation into three stages based on the Cu x-ray intensity of cuproproteins that had accumulated in hepatocyte lysosomes...
journal_title：Nagoya journal of medical science
pub_type： 杂志文章
doi：
更新日期：1993-03-01 00:00:00
abstract：PURPOSE:To clarify whether the benefit of a reduced effective scan width obtained using a smaller pitch outweighs the disadvantage of increased noise in the application of a subsecond helical CT to mass screenings for lung cancer. MATERIALS AND METHODS:Twenty-two helical CT scans of the lung were obtained in 11 health...
journal_title：Nagoya journal of medical science
pub_type： 杂志文章
doi：
更新日期：2006-06-01 00:00:00
abstract：:Disorientation responses of animals exposed to microgravity produced by parabolic aircraft flights and also in-space experiments were reviewed. Disoriented postures in floating are largely species-dependent. Reflexive lowered tone of gravity-bearing extensor muscles by labyrinthectomy is not seen in mammals (hamster a...
journal_title：Nagoya journal of medical science
pub_type： 杂志文章
doi：
更新日期：1995-12-01 00:00:00
abstract：:Surgeons focus on the period of absence from work during the initial treatment of breast cancer. The aim of this study was to determine surgeons' perceptions and awareness regarding the necessary period of absence from work during breast cancer treatment. We created a questionnaire for all surgeons involved in breast ...
journal_title：Nagoya journal of medical science
pub_type： 杂志文章
doi：
更新日期：2014-08-01 00:00:00
abstract：:The biological effects of static magnetic fields, and their combined effects with ionizing radiation, were studied using a cultured mammalian cell line (FM3A). The three different modes of magnetic fields evaluated in this report were the 0.3 Tesla (T) field with a gradient of 0.3T/m, the 0.7T field with a gradient of...
journal_title：Nagoya journal of medical science
pub_type： 杂志文章
doi：
更新日期：1995-12-01 00:00:00
abstract：:Research into the molecular genetics and pathomechanisms of ichthyoses have advanced considerably, resulting in the identification of several causative genes and molecules underlying the disease. In 2009, the First Ichthyosis Consensus Conference was held to establish a consensus for the nomenclature and classificatio...
journal_title：Nagoya journal of medical science
pub_type： 杂志文章,评审
doi：
更新日期：2011-08-01 00:00:00
abstract：:Accessibility to health care services in Thailand is generally good, but a few areas in the country are difficult to reach. The purpose of this study was to investigate the accessibility of health care services in rural and remote areas of Thailand. It was conducted in 16 remote villages within a catchment area with a...
journal_title：Nagoya journal of medical science
pub_type： 杂志文章
doi：
更新日期：2013-08-01 00:00:00
abstract：:Dermal sinus tracts (DSTs) of the cervical and thoracic spine are extremely rare, particularly in adult patients because diagnosis is typically made in the early stage after birth by pediatricians. These cases should be treated surgically as soon as possible to prevent neurological sequelae. This report describes two ...
journal_title：Nagoya journal of medical science
pub_type：
doi：10.18999/nagjms.82.3.567
更新日期：2020-08-01 00:00:00
abstract：:Surgical intervention for the treatment of acetabular fracture non-union is often challenging. Here, we present a case of acetabular fracture non-union with pelvic discontinuity in a patient who underwent cemented total hip arthroplasty (THA) after intra- and extra-articular plate fixation. The case was a 70-year-old ...
journal_title：Nagoya journal of medical science
pub_type： 杂志文章
doi：10.18999/nagjms.81.1.113
更新日期：2019-02-01 00:00:00
abstract：:Neoadjuvant chemotherapy (NAC) using the combination of anthracycline and taxanes is the standard regimen for patients with primary breast cancer. Among the taxanes, conventional paclitaxel (PTX) and docetaxel have usually been adopted in the neoadjuvant or adjuvant setting. Nanoparticle albumin-bound paclitaxel (nab-...
journal_title：Nagoya journal of medical science
pub_type： 杂志文章
doi：10.18999/nagjms.82.3.457
更新日期：2020-08-01 00:00:00
abstract：BACKGROUND:Copper toxicity steadily affects the livers of patients with Wilson disease. However, the toxic effect of copper on serum aspartate and alanine aminotransferase levels remains to be clarified as a prerequisite for diagnostic tests. The clinical records of 33 cases were analyzed to clarify the natural history...
journal_title：Nagoya journal of medical science
pub_type： 杂志文章
doi：
更新日期：2014-02-01 00:00:00
