Derangements of liver tissue bioenergetics in concanavalin A-induced hepatitis.

Abstract:

BACKGROUND:A novel in vitro system was employed to investigate liver tissue respiration (mitochondrial O2 consumption) in mice treated with concanavalin A (Con A). This study aimed to investigate hepatocyte bioenergetics in this well-studied hepatitis model. METHODS:C57Bl/6 and C57Bl/6 IFN-γ-/- mice were injected intravenously with 12 mg ConA/kg. Liver specimens were collected at various timepoints after injection and analyzed for cellular respiration and caspase activation. Serum was analyzed for interferon-gamma (IFN-γ) and aminotransferases. Fluorescence activated cell sorting analysis was used to determine the phenotype of infiltrating cells, and light and electron microscopy were used to monitor morphological changes. Phosphorescence analyzer that measured dissolved O2 as function of time was used to evaluate respiration. RESULTS:In sealed vials, O2 concentrations in solutions containing liver specimen and glucose declined linearly with time, confirming zero-order kinetics of hepatocyte respiration. O2 consumption was inhibited by cyanide, confirming the oxidation occurred in the respiratory chain. Enhanced liver respiration (by ≈68%, p<0.02) was noted 3 hr after ConA treatment, and occurred in conjunction with limited cellular infiltrations around the blood vessels. Diminished respiration (by ≈30%, p=0.005) was noted 12 hr after ConA treatment, and occurred in conjunction with deranged mitochondria, areas of necrosis, and prominent infiltrations with immune cells, most significantly, CD3+NKT+ cells. Increases in intracellular caspase activity and serum IFN-γ and aminotransferase levels were noted 3 hr after ConA treatment and progressed with time. The above-noted changes were less pronounced in C57Bl/6 IFN-γ-/- mice treated with ConA. CONCLUSIONS:Based on these results, liver tissue bioenergetics is increased 3 hr after ConA exposure. This effect is driven by the pathogenesis of the disease, in which IFN-γ and other cytokines contribute to. Subsequent declines in liver bioenergetics appear to be a result of necrosis and active caspases targeting the mitochondria within hepatocytes.

journal_name

BMC Gastroenterol

journal_title

BMC gastroenterology

authors

Al-Shamsi M,Shahin A,Mensah-Brown EP,Souid AK

doi

10.1186/1471-230X-13-6

subject

Has Abstract

pub_date

2013-01-12 00:00:00

pages

6

issn

1471-230X

pii

1471-230X-13-6

journal_volume

13

pub_type

杂志文章
  • Endocytoscopic findings of lymphomas of the stomach.

    abstract:BACKGROUND:The gastric lesions of various lymphomas were observed at the cellular level using endocytoscopy. METHODS:Endocytoscopy and magnifying endoscopy with narrow band imaging (NBI) were performed in 17 patients with lymphomas of the stomach. The lesions consisted of 7 with low-grade mucosa-associated lymphoid ti...

    journal_title:BMC gastroenterology

    pub_type: 杂志文章

    doi:10.1186/1471-230X-13-174

    authors: Isomoto H,Matsushima K,Hayashi T,Imaizumi Y,Shiota J,Ishii H,Minami H,Ohnita K,Takeshima F,Shikuwa S,Miyazaki Y,Nakao K

    更新日期:2013-12-26 00:00:00

  • A meta-analysis of the association between IL28B polymorphisms and infection susceptibility of hepatitis B virus in Asian population.

    abstract:BACKGROUND:Several association studies with small sample sizes of two SNPs in IL28BB (rs12979860 and rs8099917) showed inconsistent results, so in this study, we aim to evaluate the association between the two SNPs and infection susceptibility of Hepatitis B Virus (HBV) in Asian population, especially in Chinese popula...

    journal_title:BMC gastroenterology

    pub_type: 杂志文章,meta分析

    doi:10.1186/s12876-015-0286-2

    authors: Chen J,Wang W,Li X,Xu J

    更新日期:2015-05-12 00:00:00

  • Genetic deletion of dectin-1 does not affect the course of murine experimental colitis.

    abstract:BACKGROUND:It is believed that inflammatory bowel diseases (IBD) result from an imbalance in the intestinal immune response towards the luminal microbiome. Dectin-1 is a widely expressed pattern recognition receptor that recognizes fungi and upon recognition it mediates cytokine responses and skewing of the adaptive im...

    journal_title:BMC gastroenterology

    pub_type: 杂志文章

    doi:10.1186/1471-230X-12-33

    authors: Heinsbroek SE,Oei A,Roelofs JJ,Dhawan S,te Velde A,Gordon S,de Jonge WJ

    更新日期:2012-04-16 00:00:00

  • Value of routine test for identifying colorectal cancer from patients with nonalcoholic fatty liver disease.

    abstract:BACKGROUND:Nonalcoholic fatty liver disease (NAFLD) is a risk factor for colorectal neoplasms. Our goal is to explore the relationship between NAFLD and colorectal cancer (CRC) and to analyze potential indicators for screening CRC in NAFLD based on clinical big data. METHODS:Demographic information and routine clinica...

    journal_title:BMC gastroenterology

    pub_type: 杂志文章

    doi:10.1186/s12876-020-01327-7

    authors: Yang R,Chen Y,Chen X

    更新日期:2020-06-09 00:00:00

  • Epstein-Barr virus infection is associated with a higher Child-Pugh score and may predict poor prognoses for patients with liver cirrhosis.

    abstract:BACKGROUND:Studies on Epstein-Barr virus (EBV) have focused mostly on neoplastic disease. Few studies have considered immunocompetent patients who are not severely immunocompromised. Liver cirrhosis is associated with various levels of immune dysfunction. In the current study, we determined EBV infection rates, the inf...

    journal_title:BMC gastroenterology

    pub_type: 杂志文章

    doi:10.1186/s12876-019-1021-1

    authors: Hu J,Zhang X,Yu G,Cai H,Gu J,Hu M,Xiang D,Lian J,Yu L,Jia H,Zhang Y,Yang Y

    更新日期:2019-06-18 00:00:00

  • The role of age in predicting the outcome of caustic ingestion in adults: a retrospective analysis.

    abstract:BACKGROUND:Although the outcomes of caustic ingestion differ between children and adults, it is unclear whether such outcomes differ among adults as a function of their age. This retrospective study was performed to ascertain whether the clinical outcomes of caustic ingestion differ significantly between elderly and no...

    journal_title:BMC gastroenterology

    pub_type: 杂志文章

    doi:10.1186/1471-230X-11-72

    authors: Chang JM,Liu NJ,Pai BC,Liu YH,Tsai MH,Lee CS,Chu YY,Lin CC,Chiu CT,Cheng HT

    更新日期:2011-06-14 00:00:00

  • Longitudinal study of gastroesophageal reflux and erosive tooth wear.

    abstract:BACKGROUND:Approximately 60% of patients presenting to dentists with erosive tooth wear have significant gastroesophageal reflux (GERD), despite minor reflux symptoms. No longitudinal studies of reflux-associated erosive tooth wear and of reflux characteristics have been reported to date. The aim of this study was to c...

    journal_title:BMC gastroenterology

    pub_type: 杂志文章

    doi:10.1186/s12876-017-0670-1

    authors: Wilder-Smith CH,Materna A,Martig L,Lussi A

    更新日期:2017-10-25 00:00:00

  • Faecal calprotectin: factors affecting levels and its potential role as a surrogate marker for risk of development of Crohn's Disease.

    abstract:BACKGROUND:Faecal calprotectin (FC) is one of the most widely used non-invasive tests for the diagnosis and assessment of Crohn's disease (CD) activity. Despite this, factors other than disease activity which affect levels have not been extensively reviewed. This is of importance when using FC in the diagnostic setting...

    journal_title:BMC gastroenterology

    pub_type: 杂志文章,评审

    doi:10.1186/s12876-016-0535-z

    authors: Mendall MA,Chan D,Patel R,Kumar D

    更新日期:2016-10-07 00:00:00

  • Real life results in using 5-ASA for maintaining mild to moderate UC patients in Japan, a multi-center study, OPTIMUM Study.

    abstract:BACKGROUND:Efficacy of maintenance therapy in ulcerative colitis (UC) in the remission stage has been reported to depend on release profile or dosing regimen of oral 5-aminosalicylic acid (5-ASA) products used. Aim of this study is to investigate real life results in using oral 5-ASA products for maintaining mild to mo...

    journal_title:BMC gastroenterology

    pub_type: 杂志文章,多中心研究

    doi:10.1186/s12876-017-0604-y

    authors: Nagahori M,Kochi S,Hanai H,Yamamoto T,Nakamura S,Omuro S,Watanabe M,Hibi T,OPTIMUM Study Group.

    更新日期:2017-04-04 00:00:00

  • Narrow-band imaging and high-definition white-light endoscopy in patients with serrated lesions not fulfilling criteria for serrated polyposis syndrome: a randomized controlled trial with tandem colonoscopy.

    abstract:BACKGROUND:It is unknown whether narrow-band imaging (NBI) could be more effective than high-definition white-light endoscopy (HD-WLE) in detecting serrated lesions in patients with prior serrated lesions > 5 mm not completely fulfilling serrated polyposis syndrome (SPS) criteria. METHODS:We conducted a randomized, cr...

    journal_title:BMC gastroenterology

    pub_type: 杂志文章,随机对照试验

    doi:10.1186/s12876-020-01257-4

    authors: Riu Pons F,Andreu M,Naranjo D,Álvarez-González MA,Seoane A,Dedeu JM,Barranco L,Bessa X

    更新日期:2020-04-16 00:00:00

  • Faecal calprotectin level for assessing endoscopic activity and predicting future clinical course in patients with moderately active ulcerative colitis undergoing granulomonocytapheresis: a prospective cohort study.

    abstract:BACKGROUND:Calprotectin is a stable neutrophil protein, which can be measured in faecal samples. The faecal level of calprotectin increases during disease activity in ulcerative colitis (UC). Nonetheless, the relevance of faecal calprotectin (FC) measurement during granulomonocytapheresis (GMA) for UC has not yet been ...

    journal_title:BMC gastroenterology

    pub_type: 杂志文章

    doi:10.1186/s12876-018-0853-4

    authors: Shimoyama T,Yamamoto T,Umegae S,Matsumoto K

    更新日期:2018-08-01 00:00:00

  • Clinical predictors of incident gallstone disease in a Chinese population in Taipei, Taiwan.

    abstract:BACKGROUND:Gallstone disease (GSD) is a common gastrointestinal disorder throughout the world. The authors explored the incidence of GSD in Taiwan and its condition-associated predictive factors. METHODS:The initial study cohort comprised 2386 healthy adult participants, who were voluntarily admitted to a teaching hos...

    journal_title:BMC gastroenterology

    pub_type: 杂志文章

    doi:10.1186/1471-230X-14-83

    authors: Chen JY,Hsu CT,Liu JH,Tung TH

    更新日期:2014-04-28 00:00:00

  • Gynecomastia during imatinib mesylate treatment for gastrointestinal stromal tumor: a rare adverse event.

    abstract:BACKGROUND:Imatinib mesylate has been the standard therapeutic treatment for chronic myeloid leukemia, advanced and metastatic gastrointestinal stromal tumor (GIST). It is well tolerated with mild adverse effects. Gynecomastia development during the course of treatment has been rarely reported. METHODS:Ninety-eight pa...

    journal_title:BMC gastroenterology

    pub_type: 杂志文章

    doi:10.1186/1471-230X-11-116

    authors: Liu H,Liao G,Yan Z

    更新日期:2011-11-02 00:00:00

  • Steroid-refractory ulcerative colitis treated with corticosteroids, metronidazole and vancomycin: a case report.

    abstract:BACKGROUND:Increasing evidence elucidating the pathogenic mechanisms of ulcerative colitis (UC) has accumulated and the disease is widely assumed to be the consequence of genetic susceptibility and an abnormal immune response to commensal bacteria. However evidence regarding an infectious etiology in UC remains elusive...

    journal_title:BMC gastroenterology

    pub_type: 杂志文章

    doi:10.1186/1471-230X-5-3

    authors: Miner J,Gillan MM,Alex P,Centola M

    更新日期:2005-01-24 00:00:00

  • Gastric cancer is associated with NOS2 -954G/C polymorphism and environmental factors in a Brazilian population.

    abstract:BACKGROUND:Gastric cancer can progress from a chronic inflammation of the gastric mucosa resulting from Helicobacter pylori infection that activates the inflammatory response of the host. Therefore, polymorphisms in genes involved in the inflammatory response, such as inducible nitric oxide synthase (NOS2), have been i...

    journal_title:BMC gastroenterology

    pub_type: 杂志文章

    doi:10.1186/1471-230X-10-64

    authors: Jorge YC,Duarte MC,Silva AE

    更新日期:2010-06-17 00:00:00

  • Epidermoid cyst in an intrapancreatic accessory spleen in the pancreas head: a case report.

    abstract:BACKGROUND:An epidermoid cyst in an intrapancreatic accessory spleen (ECIPAS) in the pancreas head is an extremely rare condition. The natural course of this condition is not well known, and it is difficult to diagnose before surgery due to the lack of specific imaging findings. CASE PRESENTATION:A tumor was found in ...

    journal_title:BMC gastroenterology

    pub_type: 杂志文章

    doi:10.1186/s12876-020-01540-4

    authors: Ko HJ,Shim JR,Lee TB,Choi BH,Lee JH,Ryu JH,Yang K

    更新日期:2020-11-20 00:00:00

  • Real-world clinical effectiveness and safety of vedolizumab and anti-tumor necrosis factor alpha treatment in ulcerative colitis and Crohn's disease patients: a German retrospective chart review.

    abstract:BACKGROUND:Real-world comparisons of biologic treatment outcomes for ulcerative colitis (UC) or Crohn's disease (CD) patients are limited. We sought to evaluate the real-world effectiveness of vedolizumab (VDZ) and anti-tumor necrosis factor alpha (anti-TNFα) in UC and CD patients in Germany. METHODS:A retrospective c...

    journal_title:BMC gastroenterology

    pub_type: 杂志文章

    doi:10.1186/s12876-020-01332-w

    authors: Helwig U,Mross M,Schubert S,Hartmann H,Brandes A,Stein D,Kempf C,Knop J,Campbell-Hill S,Ehehalt R

    更新日期:2020-07-08 00:00:00

  • Paraneoplastic pemphigus and myasthenia gravis as the first manifestations of a rare case of pancreatic follicular dendritic cell sarcoma: CT findings and review of literature.

    abstract:BACKGROUND:Follicular dendritic cell sarcoma (FDCS) is a rare neoplasm that originates from follicular dendritic cells in lymphoid tissue while paraneoplastic pemphigus (PNP) is an autoimmune blistering disease associated with neoplasms. Pancreatic FDCS associated with PNP and myasthenia gravis (MG) is even rarer and h...

    journal_title:BMC gastroenterology

    pub_type: 杂志文章,评审

    doi:10.1186/s12876-019-1008-y

    authors: Lu T,Song B,Pu H,Li X,Chen Q,Yang C

    更新日期:2019-06-14 00:00:00

  • The addition of a pH-sensitive gel improves microemulsion stability for the targeted removal of colonic ammonia.

    abstract:BACKGROUND:We prepared an oral W/O microemulsion for the removal of colonic ammonia (ME-RCA). The effect of this microemulsion was influenced by the digestion process in the gastrointestinal tract. In this paper, we aim to show that stability was improved by using a microemulsion-based gel for the removal of colonic am...

    journal_title:BMC gastroenterology

    pub_type: 杂志文章

    doi:10.1186/1471-230X-11-50

    authors: Wang AH,Duan ZJ,Tian G,Lu D,Zhang WJ,He GH,Fang GH

    更新日期:2011-05-10 00:00:00

  • Early stage signet ring cell carcinoma of the colon examined by magnifying endoscopy with narrow-band imaging: a case report.

    abstract:BACKGROUND:Signet ring cell carcinoma of the colon and rectum is rare, and most cases are detected at an advanced stage. We present a case of primary signet ring cell carcinoma detected at an early stage by magnifying endoscopy with narrow-band imaging (NBI) and crystal violet staining. CASE PRESENTATION:A 73-year-old...

    journal_title:BMC gastroenterology

    pub_type: 杂志文章

    doi:10.1186/s12876-015-0317-z

    authors: Ohnita K,Isomoto H,Akashi T,Hashiguchi K,Matsushima K,Minami H,Akazawa Y,Yamaguchi N,Takeshima F,To K,Takeshita H,Yasui H,Abe K,Nakao K

    更新日期:2015-07-24 00:00:00

  • A case report on a patient suffering from recurrent vomiting episodes, whose condition improved markedly during pregnancy and breast feeding.

    abstract:BACKGROUND:The normal physiology of the gastrointestinal tract has been only cursorily examined. Consequently, the pathophysiology of disturbances of the gastrointestinal functions is poorly known. Recurrent vomiting is one of many functional conditions for which it is difficult to find an explanation and to treat. In ...

    journal_title:BMC gastroenterology

    pub_type: 杂志文章

    doi:10.1186/1471-230X-6-28

    authors: Ohlsson B

    更新日期:2006-10-21 00:00:00

  • Abdominal migraine in children: association between gastric motility parameters and clinical characteristics.

    abstract:BACKGROUND:Approximately 0.2-1 % of children suffers from abdominal migraine (AM). Pathophysiology of AM has not been adequately studied. This study evaluated gastric motility in children with AM. METHODS:Seventeen children (6 boys), within an age range of 4-15 years, referred to a tertiary care paediatric unit, North...

    journal_title:BMC gastroenterology

    pub_type: 杂志文章

    doi:10.1186/s12876-016-0435-2

    authors: Devanarayana NM,Rajindrajith S,Benninga MA

    更新日期:2016-02-29 00:00:00

  • Gall bladder wall thickening as non-invasive screening parameter for esophageal varices - a comparative endoscopic - sonographic study.

    abstract:BACKGROUND:The mortality due to hemorrhage of esophageal varices (EV) is still high. The predominant cause for EV is liver cirrhosis, which has a high prevalence in Western Europe. Therefore, non-invasive screening markers for the presence of EV are of interest. Here, we aim to investigate whether non-inflammatory gall...

    journal_title:BMC gastroenterology

    pub_type: 杂志文章

    doi:10.1186/s12876-018-0852-5

    authors: Tsaknakis B,Masri R,Amanzada A,Petzold G,Ellenrieder V,Neesse A,Kunsch S

    更新日期:2018-08-02 00:00:00

  • Unusual manifestation of Erdheim-Chester disease.

    abstract:BACKGROUND:Erdheim-Chester disease (ECD) is a rare multisystem non-Langerhans cell histiocytosis that is characterized histologically by xanthogranulomatous infiltrates and radiologically by symmetrical sclerosis of long bones. The xanthomatous process is characterized by prominent foamy histiocytes staining positive f...

    journal_title:BMC gastroenterology

    pub_type: 杂志文章

    doi:10.1186/1471-230X-11-77

    authors: Pan A,Doyle T,Schlup M,Lubcke R,Schultz M

    更新日期:2011-06-22 00:00:00

  • Association between ERCC1 and TS mRNA levels and disease free survival in colorectal cancer patients receiving oxaliplatin and fluorouracil (5-FU) adjuvant chemotherapy.

    abstract:BACKGROUND:Aim was to explore the association of ERCC1 and TS mRNA levels with the disease free survival (DFS) in Chinese colorectal cancer (CRC) patients receiving oxaliplatin and 5-FU based adjuvant chemotherapy. METHODS:Total 112 Chinese stage II-III CRC patients were respectively treated by four different chemothe...

    journal_title:BMC gastroenterology

    pub_type: 杂志文章

    doi:10.1186/1471-230X-14-154

    authors: Li S,Zhu L,Yao L,Xia L,Pan L

    更新日期:2014-08-29 00:00:00

  • Clinical and molecular characterization of a patient with mitochondrial Neurogastrointestinal Encephalomyopathy.

    abstract:BACKGROUND:Mitochondrial neurogastrointestinal encephalomyopathy (MNGIE) is a rare autosomal recessive disorder caused by mutations in TYMP gene, encoding nuclear thymidine phosphorylase (TP). MNGIE mainly presents with gastrointestinal symptoms and is mostly misdiagnosed in many patients as malabsorption syndrome, inf...

    journal_title:BMC gastroenterology

    pub_type: 杂志文章

    doi:10.1186/s12876-020-01280-5

    authors: Habibzadeh P,Silawi M,Dastsooz H,Bahramjahan S,Ezzatzadegan Jahromi S,Ostovan VR,Yavarian M,Mofatteh M,Faghihi MA

    更新日期:2020-05-08 00:00:00

  • How do patients with inflammatory bowel disease want their biological therapy administered?

    abstract:BACKGROUND:Infliximab is usually administered by two monthly intravenous (iv) infusions, therefore requiring visits to hospital. Adalimumab is administered by self subcutaneous (sc) injections every other week. Both of these anti-TNF drugs appear to be equally efficacious in the treatment of Crohn's Disease and therefo...

    journal_title:BMC gastroenterology

    pub_type: 杂志文章

    doi:10.1186/1471-230X-10-1

    authors: Allen PB,Lindsay H,Tham TC

    更新日期:2010-01-10 00:00:00

  • Early effect of oral administration of omeprazole with mosapride as compared with those of omeprazole alone on the intragastric pH.

    abstract:BACKGROUND:The ideal medication for acid-related diseases should have a rapid onset of action to promote hemostasis and cause efficient resolution of symptoms. The aim of our study was to comparatively investigate the inhibitory effect on gastric acid secretion of a single oral administration of omeprazole plus mosapri...

    journal_title:BMC gastroenterology

    pub_type: 杂志文章,随机对照试验

    doi:10.1186/1471-230X-12-25

    authors: Iida H,Inamori M,Fujii T,Sekino Y,Endo H,Hosono K,Nonaka T,Koide T,Takahashi H,Yoneda M,Goto A,Abe Y,Kobayashi N,Kirikoshi H,Kubota K,Saito S,Gotoh E,Maeda S,Nakajima A

    更新日期:2012-03-26 00:00:00

  • Covered versus uncovered self-expandable metallic stents for palliation of malignant gastric outlet obstruction: a systematic review and meta-analysis.

    abstract:BACKGROUND:Self-expandable metallic stents (SEMSs) are widely used for palliation of malignant gastric outlet obstruction (GOO). There are two types of SEMS, covered and uncovered, each with its own advantages and disadvantages. We aimed to compare the efficacy and safety between uncovered and covered SEMSs in the pall...

    journal_title:BMC gastroenterology

    pub_type: 杂志文章,meta分析,评审

    doi:10.1186/1471-230X-14-170

    authors: Pan YM,Pan J,Guo LK,Qiu M,Zhang JJ

    更新日期:2014-09-30 00:00:00

  • Collagenous colitis presenting as spontaneous perforation in an 80 year old woman: Report of a Case.

    abstract:BACKGROUND:Perforation of the colon occurring during or shortly following colonoscopy or barium enema is a rare complication of collagenous colitis (CC). "Spontaneous" perforation in CC, in which no instigating factor is identified, is even less common, with only five cases reported to date. We report herein an additio...

    journal_title:BMC gastroenterology

    pub_type: 杂志文章

    doi:10.1186/s12876-016-0533-1

    authors: Mitchell A,Dugas A

    更新日期:2016-10-06 00:00:00