Abstract：
:Lipid droplets (LDs) are globular subcellular structures that store neutral lipids. LDs are closely associated with the endoplasmic reticulum (ER) and are limited by a phospholipid monolayer harboring a specific set of proteins. Most of these proteins associate with LDs through either an amphipathic helix or a membrane-embedded hairpin motif. Here, we address the question of whether integral membrane proteins can localize to the surface of LDs. To test this, we fused perilipin 3 (PLIN3), a mammalian LD-targeted protein, to ER-resident proteins. The resulting fusion proteins localized to the periphery of LDs in both yeast and mammalian cells. This peripheral LD localization of the fusion proteins, however, was due to a redistribution of the ER around LDs, as revealed by bimolecular fluorescence complementation between ER- and LD-localized partners. A LD-tethering function of PLIN3-containing membrane proteins was confirmed by fusing PLIN3 to the cytoplasmic domain of an outer mitochondrial membrane protein, OM14. Expression of OM14-PLIN3 induced a close apposition between LDs and mitochondria. These data indicate that the ER-LD junction constitutes a barrier for ER-resident integral membrane proteins.
journal_name
J Cell Scijournal_title
Journal of cell scienceauthors
Khaddaj R,Mari M,Cottier S,Reggiori F,Schneiter Rdoi
10.1242/jcs.256206keywords:
["\n Saccharomyces cerevisiae\n ","Endoplasmic reticulum","Lipid droplets","Perilipins","Seipin","Steryl esters","Triacylglycerols"]subject
Has Abstractpub_date
2022-03-01 00:00:00issue
5eissn
0021-9533issn
1477-9137pii
268334journal_volume
135pub_type
杂志文章abstract：:Structures resembling nuclei but depleted of protein may be released by gently lysing cells in solutions containing non-ionic detergents and high concentrations of salt. These nucleoids sediment in gradients containing intercalating agents in a manner characteristic of DNA that is intact, supercoiled and circular. The...
journal_title：Journal of cell science
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pub_type： 杂志文章
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更新日期：1999-04-01 00:00:00
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journal_title：Journal of cell science
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journal_title：Journal of cell science
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更新日期：1976-12-01 00:00:00
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journal_title：Journal of cell science
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doi：
更新日期：1999-06-01 00:00:00
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doi：
更新日期：1999-06-01 00:00:00
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pub_type： 杂志文章
doi：
更新日期：1999-06-01 00:00:00
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pub_type： 杂志文章
doi：
更新日期：1976-12-01 00:00:00
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pub_type： 杂志文章
doi：
更新日期：1999-07-01 00:00:00
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pub_type： 杂志文章
doi：
更新日期：1999-10-01 00:00:00
