Abstract:
:The currently available chemotherapeutic regimens against gastric cancer are not very effective, leading to high recurrence and poor survival. Resveratrol is a naturally occurring polyphenol with potent apoptosis-inducing activity. However, the mechanism underlying its actions remains unknown. In the present study, human gastric adenocarcinoma SGC7901 cells were treated with resveratrol (0, 25, 50, 100 and 200 μmol/L) for 48 h, and cellular apoptosis DNA damage were determined. In certain experiments, cells were incubated with superoxide dismutase (100 U/mL), catalase (300 U/mL) or sirtinol (10 μmol/L) to determine the role of reactive oxygen species (ROS) and sirtuin1 in resveratrol-induced cellular apoptosis. Treatment with resveratrol (50-200 μmol/L) for 48 h significantly induced apoptosis and DNA damage in human gastric cancer SGC7901 cells. This was due to the increased generation of ROS following resveratrol treatment because incubation of cells with superoxide dismutase (100 U/mL) or catalase (300 U/mL) attenuated resveratrol-induced cellular apoptosis. Interestingly, treatment with resveratrol (25-200 μmol/L) did not affect the level and activity of sirtuin1, whereas the sirtuin1 inhibitor sirtinol (10 μmol/L) significantly reduced sirtuin1 activity. Furthermore, treatment with sirtinol (10 μmol/L) did not have any effect on apoptosis induced by resveratrol. These data provide evidence that resveratrol induces apoptosis via ROS, but independent of sirtuin1, in the human gastric cancer cell line SGC7901.
journal_name
Clin Exp Pharmacol Physioljournal_title
Clinical and experimental pharmacology & physiologyauthors
Wang Z,Li W,Meng X,Jia Bdoi
10.1111/j.1440-1681.2011.05660.xsubject
Has Abstractpub_date
2012-03-01 00:00:00pages
227-32issue
3eissn
0305-1870issn
1440-1681journal_volume
39pub_type
杂志文章abstract::1. In the rectum and distal nephron, sodium reabsorption is mediated by the amiloride-sensitive epithelial sodium channel (ENaC). The ENaC-mediated sodium transport is electrogenic and creates an amiloride-sensitive transepithelial potential difference (PD). 2. We have evaluated the salt- and angiotensin (Ang)II-depen...
journal_title:Clinical and experimental pharmacology & physiology
pub_type: 杂志文章
doi:10.1046/j.1440-1681.2000.03204.x
更新日期:2000-01-01 00:00:00
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journal_title:Clinical and experimental pharmacology & physiology
pub_type: 杂志文章
doi:10.1111/1440-1681.12860
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journal_title:Clinical and experimental pharmacology & physiology
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doi:10.1111/j.1440-1681.1991.tb01464.x
更新日期:1991-05-01 00:00:00
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journal_title:Clinical and experimental pharmacology & physiology
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pub_type: 杂志文章,评审
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journal_title:Clinical and experimental pharmacology & physiology
pub_type: 杂志文章
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journal_title:Clinical and experimental pharmacology & physiology
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pub_type: 杂志文章
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更新日期:1980-03-01 00:00:00
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pub_type: 杂志文章
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更新日期:2020-09-01 00:00:00
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journal_title:Clinical and experimental pharmacology & physiology
pub_type: 杂志文章
doi:10.1111/j.1440-1681.1994.tb02546.x
更新日期:1994-06-01 00:00:00
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pub_type: 临床试验,杂志文章,随机对照试验
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更新日期:2002-08-01 00:00:00