当前位置： SCI文献检索 > JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH期刊下所有文献 > Effects of DNMT1 silencing on malignant phenotype and methylated gene expression in cervical cancer cells.

Effects of DNMT1 silencing on malignant phenotype and methylated gene expression in cervical cancer cells.

Abstract：

BACKGROUND:DNA methylation has been widely used in classification, early diagnosis, therapy and prediction of metastasis as well as recurrence of cervical cancer. DNMT methyltransferase 1 (DNMT1), which plays a significant role in maintaining DNA methylation status and regulating the expression of tumor suppressor genes. The aim of this research was to investigate the relationship between DNMT1 and abnormal methylation of tumor suppressor genes and malignant phenotype in cervical cancer. METHODS:Levels of DNMT1 mRNA and protein were detected using qPCR and Western blot, respectively. Cell proliferation was analyzed by MTT and apoptosis was performed by Annexin V-FITC/PI double staining flow cytometry, respectively. MeDIP-qPCR and qPCR were performed to measure demethylation status and mRNA re-expression level of 7 tumor-suppressor genes (CCNA1, CHFR, FHIT, PAX1, PTEN, SFRP4, TSLC1) in Hela and Siha cells after silencing DNMT1. RESULTS:The average expression levels of DNMT1 mRNA and protein in Hela and Siha cells were decreased significantly compared with control group. The flow cytometry and MTT results showed that Hela and Siha cells apoptosis rates and cell viabilities were 19.4 ± 2.90%, 25.7 ± 3.92% as well as 86.7 ± 3.12%, 84.16 ± 2.67% respectively 48 h after transfection (P < 0.01). Furthermore, the promoter methylation of five tumor suppressor genes was decreased with the increased mRNA expression after silencing DNMT1, whereas there were no significant changes in PTEN and FHIT genes in Hela cells, and CHFR and FHIT genes in Siha cells. CONCLUSIONS:Our experimental results demonstrate that methylation status of DNMT1 can influence several important tumor suppressor genes activity in cervical tumorigenesis and may have the potential to become an effective target for treatment of cervical cancer.

journal_name

J Exp Clin Cancer Res

journal_title

Journal of experimental & clinical cancer research : CR

authors

Zhang Y,Chen FQ,Sun YH,Zhou SY,Li TY,Chen R

doi

10.1186/1756-9966-30-98

subject

Has Abstract

pub_date

2011-10-17 00:00:00

pages

98

eissn

0392-9078

issn

1756-9966

pii

1756-9966-30-98

journal_volume

30

pub_type

杂志文章

相关文献

JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH文献大全
  • Inhibition of ATRA-induced myeloid differentiation in acute promyelocytic leukemia by a new protein tyrosine phosphatase inhibitor, 3,4-dephostatin.

    abstract：:We previously reported that the expression and the activity of SHP-1, a non-transmembrane protein tyrosine phosphatase (PTPase) increased during myeloid differentiation of an acute promyelocytic leukemia cell line (HT93) induced by all-trans retinoic acid (ATRA). To examine whether inhibition of SHP-1 activity attenua...

    journal_title：Journal of experimental & clinical cancer research : CR

    pub_type： 杂志文章

    doi：

    authors： Uesugi Y,Fuse I,Toba K,Kishi K,Hashimoto S,Furukawa T,Narita M,Takahashi M,Aizawa Y

    更新日期：2000-09-01 00:00:00

  • Overexpressed histone acetyltransferase 1 regulates cancer immunity by increasing programmed death-ligand 1 expression in pancreatic cancer.

    abstract：BACKGROUND:Pancreatic ductal adenocarcinoma is one of the leading causes of cancer-related death worldwide. Immune checkpoint blockade therapy, including anti-PD-1 and anti-PD-L1, is a new therapeutic strategy for cancer treatment but the monotherapy with PD-L1 inhibitors for pancreatic cancer is almost ineffective for...

    journal_title：Journal of experimental & clinical cancer research : CR

    pub_type： 杂志文章

    doi：10.1186/s13046-019-1044-z

    authors： Fan P,Zhao J,Meng Z,Wu H,Wang B,Wu H,Jin X

    更新日期：2019-02-01 00:00:00

  • Restoration of E-cadherin expression by selective Cox-2 inhibition and the clinical relevance of the epithelial-to-mesenchymal transition in head and neck squamous cell carcinoma.

    abstract：BACKGROUND:The epithelial-to-mesenchymal transition (EMT) accompanied by the downregulation of E-cadherin has been thought to promote metastasis. Cyclooxygenase-2 (Cox-2) is presumed to contribute to cancer progression through its multifaceted function, and recently its inverse relationship with E-cadherin was suggeste...

    journal_title：Journal of experimental & clinical cancer research : CR

    pub_type： 杂志文章

    doi：10.1186/1756-9966-33-40

    authors： Fujii R,Imanishi Y,Shibata K,Sakai N,Sakamoto K,Shigetomi S,Habu N,Otsuka K,Sato Y,Watanabe Y,Ozawa H,Tomita T,Kameyama K,Fujii M,Ogawa K

    更新日期：2014-05-10 00:00:00

  • Radiation-induced miR-208a increases the proliferation and radioresistance by targeting p21 in human lung cancer cells.

    abstract：BACKGROUND:Lung cancer has long been the most dangerous malignant tumor among males in both well developed and poorly developed countries. Radiotherapy plays a critical role in the curative management of inoperable non-small cell lung cancer (NSCLC) and is also used as a post-surgical treatment in lung cancer patients....

    journal_title：Journal of experimental & clinical cancer research : CR

    pub_type： 杂志文章

    doi：10.1186/s13046-016-0285-3

    authors： Tang Y,Cui Y,Li Z,Jiao Z,Zhang Y,He Y,Chen G,Zhou Q,Wang W,Zhou X,Luo J,Zhang S

    更新日期：2016-01-12 00:00:00

  • Molecular markers associated with outcome and metastasis in human pancreatic cancer.

    abstract：BACKGROUND:Pancreatic ductal adenocarcinoma (PDAC) is a heterogeneous cancer in which differences in survival rates might be related to a variety in gene expression profiles. Although the molecular biology of PDAC begins to be revealed, genes or pathways that specifically drive tumour progression or metastasis are not ...

    journal_title：Journal of experimental & clinical cancer research : CR

    pub_type： 杂志文章

    doi：10.1186/1756-9966-31-68

    authors： Van den Broeck A,Vankelecom H,Van Eijsden R,Govaere O,Topal B

    更新日期：2012-08-27 00:00:00

  • Nucleolar and spindle associated protein 1 promotes the aggressiveness of astrocytoma by activating the Hedgehog signaling pathway.

    abstract：BACKGROUND:The prognosis of human astrocytoma is poor, and the molecular alterations underlying its pathogenesis still needed to be elucidated. Nucleolar and spindle associated protein 1 (NUSAP1) was observed in several types of cancers, but its role in astrocytoma remained unknown. METHODS:The expression of NUSAP1 in...

    journal_title：Journal of experimental & clinical cancer research : CR

    pub_type： 杂志文章

    doi：10.1186/s13046-017-0597-y

    authors： Wu X,Xu B,Yang C,Wang W,Zhong D,Zhao Z,He L,Hu Y,Jiang L,Li J,Song L,Zhang W

    更新日期：2017-09-12 00:00:00

  • CCDC88A, a prognostic factor for human pancreatic cancers, promotes the motility and invasiveness of pancreatic cancer cells.

    abstract：BACKGROUND:Coiled-Coil Domain Containing 88A (CCDC88A) was identified as a substrate of the serine/threonine kinase Akt that is capable of binding to the actin cytoskeleton. The aim of this study was to investigate the potential role of CCDC88A in the migration and invasiveness of pancreatic ductal adenocarcinoma (PDAC...

    journal_title：Journal of experimental & clinical cancer research : CR

    pub_type： 杂志文章

    doi：10.1186/s13046-016-0466-0

    authors： Tanouchi A,Taniuchi K,Furihata M,Naganuma S,Dabanaka K,Kimura M,Watanabe R,Kohsaki T,Shimizu T,Saito M,Hanazaki K,Saibara T

    更新日期：2016-12-05 00:00:00

  • Correction to: Musashi2 promotes EGF-induced EMT in pancreatic cancer via ZEB1-ERK/MAPK signaling.

    abstract：:An amendment to this paper has been published and can be accessed via the original article. ...

    journal_title：Journal of experimental & clinical cancer research : CR

    pub_type： 杂志文章,已发布勘误

    doi：10.1186/s13046-020-01663-2

    authors： Sheng W,Shi X,Lin Y,Tang J,Jia C,Cao R,Sun J,Wang G,Zhou L,Dong M

    更新日期：2020-08-24 00:00:00

  • Epidermal growth factor (EGF) receptor-ligand based molecular staging predicts prognosis in head and neck squamous cell carcinoma partly due to deregulated EGF- induced amphiregulin expression.

    abstract：BACKGROUND:Increased expression of epidermal growth factor receptor (EGFR) and its ligands is associated with poor prognosis and chemoresistance in many carcinoma types, but its role in head and neck squamous cell carcinoma (HNSCC) is unclear. Our aim was to clarify whether mRNA expression of EGFR-ligands was linked to...

    journal_title：Journal of experimental & clinical cancer research : CR

    pub_type： 杂志文章

    doi：10.1186/s13046-016-0422-z

    authors： Gao J,Ulekleiv CH,Halstensen TS

    更新日期：2016-09-26 00:00:00

  • Long non-coding RNA small nucleolar RNA host gene 12 (SNHG12) promotes tumorigenesis and metastasis by targeting miR-199a/b-5p in hepatocellular carcinoma.

    abstract：BACKGROUND:Hepatocellular carcinoma (HCC) is third leading cause of cancer-related death globally. Evidence suggest that long non-coding RNAs (lncRNAs) have emerged as key regulators of tumorigenesis and metastasis in HCC. In this study, we investigated the functional significance of SNHG12 and explored whether SNHG12 ...

    journal_title：Journal of experimental & clinical cancer research : CR

    pub_type： 杂志文章

    doi：10.1186/s13046-016-0486-9

    authors： Lan T,Ma W,Hong Z,Wu L,Chen X,Yuan Y

    更新日期：2017-01-10 00:00:00

  • PERK/NRF2 and autophagy form a resistance mechanism against G9a inhibition in leukemia stem cells.

    abstract：BACKGROUND:The histone methyltransferase G9a has recently been identified as a potential target for epigenetic therapy of acute myeloid leukemia (AML). However, the effect of G9a inhibition on leukemia stem cells (LSCs), which are responsible for AML drug resistance and recurrence, is unclear. In this study, we investi...

    journal_title：Journal of experimental & clinical cancer research : CR

    pub_type： 杂志文章

    doi：10.1186/s13046-020-01565-3

    authors： Jang JE,Eom JI,Jeung HK,Chung H,Kim YR,Kim JS,Cheong JW,Min YH

    更新日期：2020-04-15 00:00:00

  • Carcinogenesis of intestinal-type gastric cancer and colorectal cancer is commonly accompanied by expression of brain (fetal)-type glycogen phosphorylase.

    abstract：:Our previous studies have demonstrated the significant enzymatic activity of glycogen phosphorylase (GP) in the gastric carcinoma and proliferating cells of particular intestinal metaplasia (IM). This paper reviewed the identification of the GP isoform in the gastrointestinal carcinoma, and the investigation on the ro...

    journal_title：Journal of experimental & clinical cancer research : CR

    pub_type： 杂志文章,评审

    doi：

    authors： Shimada S,Tashima S,Yamaguchi K,Matsuzaki H,Ogawa M

    更新日期：1999-03-01 00:00:00

  • Single nucleotide polymorphisms in the mitochondrial displacement loop and outcome of esophageal squamous cell carcinoma.

    abstract：BACKGROUND:Accumulation of single nucleotide polymorphisms (SNPs) in the displacement loop (D-loop) of mitochondrial DNA (mtDNA) has been described for different types of cancers and might be associated with cancer risk and disease outcome. We used a population-based series of esophageal squamous cell carcinoma (ESCC) ...

    journal_title：Journal of experimental & clinical cancer research : CR

    pub_type： 杂志文章

    doi：10.1186/1756-9966-29-155

    authors： Zhang R,Wang R,Zhang F,Wu C,Fan H,Li Y,Wang C,Guo Z

    更新日期：2010-11-26 00:00:00

  • Malignant glucagonoma. New options of treatment.

    abstract：:Few cases of malignant glucagonomas have been described in the literature. In this paper we present a case of a 77-year-old woman with necrolytic migratory erythema and high plasma glucagon and chromogranin A levels caused by a neuroendocrine tumour. An abdominal CT scan suggested a pancreatic lesion and two liver met...

    journal_title：Journal of experimental & clinical cancer research : CR

    pub_type： 杂志文章

    doi：

    authors： Appetecchia M,Ferretti E,Carducci M,Izzo F,Carpanese L,Marandino F,Terzoli E

    更新日期：2006-03-01 00:00:00

  • Isovitexin reduces carcinogenicity and stemness in hepatic carcinoma stem-like cells by modulating MnSOD and FoxM1.

    abstract：BACKGROUND:Manganese superoxide dismutase (MnSOD) upregulating FoxM1 have previously been demonstrated promoting lung cancer stemness. Isovitexin exhibits antitumor activities in various cancers. This study aimed to assess whether isovitexin inhibits hepatic carcinoma stem-like cells (HCSLCs) features via regulating Mn...

    journal_title：Journal of experimental & clinical cancer research : CR

    pub_type： 杂志文章

    doi：10.1186/s13046-019-1244-6

    authors： Cao X,Liu L,Yuan Q,Li X,Cui Y,Ren K,Zou C,Chen A,Xu C,Qiu Y,Quan M,Zhang J,Cao J,Chen X

    更新日期：2019-06-17 00:00:00

  • CT cystoscopy in the evaluation of bladder tumors.

    abstract：:The aim of the present study was to assess the role of virtual cystoscopy in the identification of bladder tumors. Fifteen patients (11 men and 4 women, median age: 61 years, range: 46-74 years) with a positive finding of bladder tumor at fiber-optic cystoscopy were studied by multislice-CT. Scans were downloaded to a...

    journal_title：Journal of experimental & clinical cancer research : CR

    pub_type： 杂志文章

    doi：

    authors： Marini M,Stasolla A,D'Aprile MR,Colaiacomo MC,Lo Mele L,Maccioni F,Iori S,Binda B,Izzo L

    更新日期：2003-03-01 00:00:00

  • Transcription factor Sp1 induces ADAM17 and contributes to tumor cell invasiveness under hypoxia.

    abstract：BACKGROUND:Expression of the Sp1 transcription factor is induced by hypoxia, and the ADAM17 promoter contains predicted Sp1 binding sites. ADAM17 contributes to hypoxic-induce invasiveness of glioma. In this study, we investigated whether Sp1 transcription factor induces ADAM17 and/or contributes to tumor cell invasive...

    journal_title：Journal of experimental & clinical cancer research : CR

    pub_type： 杂志文章

    doi：10.1186/1756-9966-28-129

    authors： Szalad A,Katakowski M,Zheng X,Jiang F,Chopp M

    更新日期：2009-09-22 00:00:00

  • Whole brain radiotherapy with radiosensitizer for brain metastases.

    abstract：PURPOSE:To study the efficacy of whole brain radiotherapy (WBRT) with radiosensitizer in comparison with WBRT alone for patients with brain metastases in terms of overall survival, disease progression, response to treatment and adverse effects of treatment. METHODS:A meta-analysis of randomized controlled trials (RCT)...

    journal_title：Journal of experimental & clinical cancer research : CR

    pub_type： 杂志文章,meta分析

    doi：10.1186/1756-9966-28-1

    authors： Viani GA,Manta GB,Fonseca EC,De Fendi LI,Afonso SL,Stefano EJ

    更新日期：2009-01-06 00:00:00

  • MicroRNA-31-5p regulates chemosensitivity by preventing the nuclear location of PARP1 in hepatocellular carcinoma.

    abstract：BACKGROUND:MicroRNAs (miRNAs) posttranscriptionally regulate gene expression and thereby contribute to the modulation of numerous complex and disease-relevant cellular processes, including cell proliferation, cell motility, apoptosis and stress response. miRNA-31-5p is encoded on a genomic fragile site, 9p21.3, which i...

    journal_title：Journal of experimental & clinical cancer research : CR

    pub_type： 杂志文章,收录出版

    doi：10.1186/s13046-018-0930-0

    authors： Que KT,Zhou Y,You Y,Zhang Z,Zhao XP,Gong JP,Liu ZJ

    更新日期：2018-11-06 00:00:00

  • Viscum album L. extracts in breast and gynaecological cancers: a systematic review of clinical and preclinical research.

    abstract：BACKGROUND:Viscum album L. extracts (VAE, European mistletoe) are a widely used medicinal plant extract in gynaecological and breast-cancer treatment. METHODS:Systematic review to evaluate clinical studies and preclinical research on the therapeutic effectiveness and biological effects of VAE on gynaecological and bre...

    journal_title：Journal of experimental & clinical cancer research : CR

    pub_type： 杂志文章,评审

    doi：10.1186/1756-9966-28-79

    authors： Kienle GS,Glockmann A,Schink M,Kiene H

    更新日期：2009-06-11 00:00:00

  • A synthetic cell-penetrating peptide derived from nuclear localization signal of EPS8 exerts anticancer activity against acute myeloid leukemia.

    abstract：BACKGROUND:Oncogenic roles of epidermal growth factor receptor pathway substrate no.8 (EPS8) have been widely reported in various tumors, making targeting of EPS8 an appealing prospect. Here, we describe the role of EPS8 in acute myeloid leukemia (AML) and consider the potential of EPS8 as an anti-AML target. Nuclear l...

    journal_title：Journal of experimental & clinical cancer research : CR

    pub_type： 杂志文章

    doi：10.1186/s13046-018-0682-x

    authors： Chen Y,Xie X,Wu A,Wang L,Hu Y,Zhang H,Li Y

    更新日期：2018-01-22 00:00:00

  • Complement C3 overexpression activates JAK2/STAT3 pathway and correlates with gastric cancer progression.

    abstract：BACKGROUND:Localized C3 deposition is a well-known factor of inflammation. However, its role in oncoprogression of gastric cancer (GC) remains obscured. This study aims to explore the prognostic value of C3 deposition and to elucidate the mechanism of C3-related oncoprogression for GC. METHODS:From August to December ...

    journal_title：Journal of experimental & clinical cancer research : CR

    pub_type： 临床试验,杂志文章

    doi：10.1186/s13046-019-1514-3

    authors： Yuan K,Ye J,Liu Z,Ren Y,He W,Xu J,He Y,Yuan Y

    更新日期：2020-01-13 00:00:00

  • Emerging roles of telomeric chromatin alterations in cancer.

    abstract：:Telomeres, the nucleoprotein structures that cap the ends of eukaryotic chromosomes, play important and multiple roles in tumorigenesis. Functional telomeres need the establishment of a protective chromatin structure based on the interplay between the specific complex named shelterin and a tight nucleosomal organizati...

    journal_title：Journal of experimental & clinical cancer research : CR

    pub_type： 杂志文章,评审

    doi：10.1186/s13046-019-1030-5

    authors： Cacchione S,Biroccio A,Rizzo A

    更新日期：2019-01-17 00:00:00

  • Cisplatin triggers cancer stem cell enrichment in platinum-resistant cells through NF-κB-TNFα-PIK3CA loop.

    abstract：BACKGROUND:Parallel to complex alteration in molecular and cellular events, enrichment of cancer stem cells (CSC) contributes significantly in deliberation and maintenance of cisplatin resistance. Cisplatin mediated CSC enrichment is well established in various cancers, yet the underlying mechanism is largely unknown. ...

    journal_title：Journal of experimental & clinical cancer research : CR

    pub_type： 杂志文章

    doi：10.1186/s13046-017-0636-8

    authors： Thakur B,Ray P

    更新日期：2017-11-23 00:00:00

  • First-line therapy in HER2 positive metastatic breast cancer: is the mosaic fully completed or are we missing additional pieces?

    abstract：:The discovery of human epidermal growth factor receptor 2 (HER2) and its role in the biology of breast cancer and the subsequent development of HER2-targeted therapies, have dramatically improved clinical outcomes for women with early-stage and advanced HER2-positive breast cancer (BC).HER-2 targeted therapies represe...

    journal_title：Journal of experimental & clinical cancer research : CR

    pub_type： 杂志文章,评审

    doi：10.1186/s13046-016-0380-5

    authors： Fabi A,Malaguti P,Vari S,Cognetti F

    更新日期：2016-06-30 00:00:00

  • Correction to: FAK-ERK activation in cell/matrix adhesion induced by the loss of apolipoprotein E stimulates the malignant progression of ovarian cancer.

    abstract：:In the original publication of this manuscript [1], Fig. 5E lower panel was incorrect due to an error in the preparation of these figures for publication. It was noticed that in the lower panel of Fig. 5E, one mouse image of ApoE-/- + PBS group (upper) was a photograph coming from ApoE-/- + BAPN pre-treatment group (l...

    journal_title：Journal of experimental & clinical cancer research : CR

    pub_type： 已发布勘误

    doi：10.1186/s13046-019-1422-6

    authors： Lai H,Zhao X,Qin Y,Ding Y,Chen R,Li G,Labrie M,Ding Z,Zhou J,Hu J,Ma D,Fang Y,Gao Q

    更新日期：2019-10-15 00:00:00

  • Circular RNA circLMO7 acts as a microRNA-30a-3p sponge to promote gastric cancer progression via the WNT2/β-catenin pathway.

    abstract：BACKGROUND:Gastric cancer (GC) is one of the most common malignant tumors worldwide. Currently, the overall survival rate of GC is still unsatisfactory despite progress in diagnosis and treatment. Therefore, studying the molecular mechanisms involved in GC is vital for diagnosis and treatment. CircRNAs, a type of nonco...

    journal_title：Journal of experimental & clinical cancer research : CR

    pub_type： 杂志文章

    doi：10.1186/s13046-020-01791-9

    authors： Cao J,Zhang X,Xu P,Wang H,Wang S,Zhang L,Li Z,Xie L,Sun G,Xia Y,Lv J,Yang J,Xu Z

    更新日期：2021-01-05 00:00:00

  • Retraction Note: Long noncoding RNA MLK7-AS1 promotes ovarian cancer cells progression by modulating miR-375/YAP1 axis.

    abstract：:An amendment to this paper has been published and can be accessed via the original article. ...

    journal_title：Journal of experimental & clinical cancer research : CR

    pub_type： 已发布勘误,撤回出版物

    doi：10.1186/s13046-020-01746-0

    authors： Yan H,Li H,Li P,Li X,Lin J,Zhu L,Silva MA,Wang X,Wang P,Zhang Z

    更新日期：2020-11-05 00:00:00

  • Ageing and the mechanisms of carcinogenesis: some practical implications.

    abstract：:Two-year protocols are commonly used for long-term assay of chemicals for carcinogenicity. However, in some cases, if animals have been exposed to tested agent from the young age and sacrificed at the age of 2, an underestimation of the carcinogenic potential of non-genotoxic agents and tumor promoters is possible. Da...

    journal_title：Journal of experimental & clinical cancer research : CR

    pub_type： 杂志文章,评审

    doi：

    authors： Anisimov VN

    更新日期：1998-09-01 00:00:00

  • Cell cycle proteins in laryngeal cancer: role in proliferation and prognosis.

    abstract：:A study of laryngeal carcinomas was performed in order to analyze (a) the expression of p53/p21, cyclin D1/cyclin E, p21/p27 (b) the relation of normal and abnormal protein expression, with the proliferation status, as determined by the expression of Ki67 and PCNA and (c) the correlation of our findings with prognosis...

    journal_title：Journal of experimental & clinical cancer research : CR

    pub_type： 杂志文章

    doi：

    authors： Peschos D,Stefanou D,Vougiouklakis T,Assimakopoulos DA,Agnantis NJ

    更新日期：2005-09-01 00:00:00