Abstract:
:Hypoparathyroidism cannot be cured by oral or intravenous calcium supplements. Using molecular biology techniques to induce the production of PTH is an ideal option to treat hypoparathyroidism. In this study, we established a recombinant plasmid encoding a mutant preproPTH with a skeletal muscle creatine kinase promoter (pCKM-mPTH). The sequence of the chimeric pCKM-mPTH gene was fully consistent with the DNA sequence reported previously and the site-directed mutagenesis was completed. Overlapping and nested PCR showed that PTH was highly expressed in and secreted from skeletal muscle cells transfected with the pCKM-mPTH plasmid: the PTH concentration in the culture medium 24 h after transfection was 26.37 pg/l. In the rat hypoparathyroidism model, serum PTH level significantly increased after injection with the pCKM-mPTH plasmid, compared with control groups (p<0.01). The effect lasted for about 30 days. Our results indicated that the recombinant mutant pCKM-mPTH plasmid was successfully constructed and was highly expressed in skeletal muscle cells. In vivo, the plasmid was introduced successfully into rat muscles and could express PTH for a decent period of time.
journal_name
J Endocrinol Investjournal_title
Journal of endocrinological investigationauthors
Liu D,Zhu Y,Yang C,Long J,Yao C,Li J,Wang Sdoi
10.3275/7966subject
Has Abstractpub_date
2012-05-01 00:00:00pages
479-84issue
5eissn
0391-4097issn
1720-8386pii
7966journal_volume
35pub_type
杂志文章abstract:PURPOSE:Remission of acromegaly has been reported after somatostatin analogs withdrawal, but not after withdrawal of combination therapy with cabergoline, and only in case reports of patients controlled by cabergoline alone. METHODS:To establish the remission rates (normal IGF-1 for age/sex: IGF-1 ≤ 1.00 xULN) after w...
journal_title:Journal of endocrinological investigation
pub_type: 杂志文章,多中心研究
doi:10.1007/s40618-016-0595-5
更新日期:2017-05-01 00:00:00
abstract::This report describes the results of the second part of the collaborative study organized by a working group sponsored by the Community Bureau of Reference of the European Community Commission. The whole study was designed to understand the causes of discrepancy among LH immunoassay methods. In the parent report we de...
journal_title:Journal of endocrinological investigation
pub_type: 临床试验,杂志文章
doi:10.1007/BF03347727
更新日期:1994-06-01 00:00:00
abstract:AIM:Serum uric acid is associated with the metabolic syndrome and its components, while its relationship with cardiovascular disease is controversial. The aim of the study was to evaluate the association between uric acid and adipokines, markers of inflammation, oxidative stress, and endothelial dysfunction, which are ...
journal_title:Journal of endocrinological investigation
pub_type: 杂志文章
doi:10.1007/BF03346397
更新日期:2008-06-01 00:00:00
abstract::A 33-year-old female presented in 1966 with striking pigmentation, typical of Addison's disease, and amenorrhea. Endocrine assessment then showed normal basal serum cortisol and urinary hydroxysteroid levels, but serum cortisol did not respond to stimulation with either exogenous ACTH or lysine vasopressin. Steroid re...
journal_title:Journal of endocrinological investigation
pub_type: 杂志文章
doi:10.1007/BF03349957
更新日期:1989-03-01 00:00:00
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journal_title:Journal of endocrinological investigation
pub_type: 杂志文章
doi:10.1007/BF03349173
更新日期:2006-01-01 00:00:00
abstract:BACKGROUND:Cadmium (Cd) is a widespread environmental pollutant that causes alterations in human health acting as endocrine disruptor. Recent data suggest that cardiovascular system might be a contamination target tissue, since Cd is found in atheromatic plaques. Thus, the purpose of this study was to evaluate the cons...
journal_title:Journal of endocrinological investigation
pub_type: 杂志文章
doi:10.1007/s40618-018-0982-1
更新日期:2019-06-01 00:00:00
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journal_title:Journal of endocrinological investigation
pub_type: 杂志文章
doi:10.1007/s40618-014-0100-y
更新日期:2014-09-01 00:00:00
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journal_title:Journal of endocrinological investigation
pub_type: 临床试验,杂志文章
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更新日期:2003-04-01 00:00:00
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journal_title:Journal of endocrinological investigation
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更新日期:2013-12-01 00:00:00
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journal_title:Journal of endocrinological investigation
pub_type: 杂志文章
doi:10.1007/BF03346365
更新日期:2008-04-01 00:00:00
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journal_title:Journal of endocrinological investigation
pub_type: 杂志文章
doi:10.1007/BF03348681
更新日期:1992-02-01 00:00:00
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journal_title:Journal of endocrinological investigation
pub_type: 杂志文章
doi:10.1007/BF03351046
更新日期:2001-06-01 00:00:00
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journal_title:Journal of endocrinological investigation
pub_type: 杂志文章
doi:10.1007/BF03343983
更新日期:2002-02-01 00:00:00
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journal_title:Journal of endocrinological investigation
pub_type: 杂志文章,评审
doi:
更新日期:2008-09-01 00:00:00
abstract::Prolactin, growth hormone and thyroid stimulating hormone responses to thyrotropin releasing hormone (TRH) and metoclopramide were determined in 12 alcoholic men with biopsy proven liver disease and were compared to those of 8 age matched normal controls. All subjects were challenged with TRH (400 micrograms) and meto...
journal_title:Journal of endocrinological investigation
pub_type: 杂志文章
doi:10.1007/BF03350515
更新日期:1982-09-01 00:00:00
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journal_title:Journal of endocrinological investigation
pub_type: 杂志文章
doi:10.3275/7332
更新日期:2011-10-01 00:00:00
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journal_title:Journal of endocrinological investigation
pub_type: 杂志文章
doi:10.1007/s40618-014-0112-7
更新日期:2014-09-01 00:00:00
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journal_title:Journal of endocrinological investigation
pub_type: 临床试验,杂志文章
doi:10.1007/BF03347358
更新日期:2003-08-01 00:00:00
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journal_title:Journal of endocrinological investigation
pub_type: 杂志文章
doi:10.1007/BF03349524
更新日期:1990-02-01 00:00:00
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journal_title:Journal of endocrinological investigation
pub_type: 杂志文章
doi:10.1007/BF03346965
更新日期:1986-12-01 00:00:00
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journal_title:Journal of endocrinological investigation
pub_type: 杂志文章
doi:10.1007/s40618-018-0875-3
更新日期:2019-01-01 00:00:00
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journal_title:Journal of endocrinological investigation
pub_type: 杂志文章,评审
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更新日期:1992-10-01 00:00:00
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journal_title:Journal of endocrinological investigation
pub_type: 杂志文章
doi:10.1007/BF03349691
更新日期:1995-01-01 00:00:00
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journal_title:Journal of endocrinological investigation
pub_type: 杂志文章
doi:10.1007/BF03348421
更新日期:1984-06-01 00:00:00
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journal_title:Journal of endocrinological investigation
pub_type: 杂志文章
doi:10.1007/BF03348184
更新日期:1987-10-01 00:00:00
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journal_title:Journal of endocrinological investigation
pub_type: 杂志文章
doi:10.1007/BF03350116
更新日期:1988-02-01 00:00:00
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journal_title:Journal of endocrinological investigation
pub_type: 杂志文章
doi:10.1007/BF03349339
更新日期:1979-10-01 00:00:00
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journal_title:Journal of endocrinological investigation
pub_type: 杂志文章
doi:10.1007/BF03346892
更新日期:1997-03-01 00:00:00
abstract::Male to female sex reversal results from failure of testis development. Mutations in the SRY gene or in other genes involved in the sexual differentiation pathway are considered to cause XY gonadal dysgenesis. The majority of the mutations in the SRY described so far are located within the SRY coding region, mainly in...
journal_title:Journal of endocrinological investigation
pub_type: 杂志文章
doi:10.1007/BF03347266
更新日期:2005-07-01 00:00:00
abstract::Differently from other metabolic conditions, most of calcium metabolism disorders are diagnosed through simple detection of both serum and urinary excretion (24-h urine collection), levels of calcium, total and ionized form, and phosphate, and of calciotropic hormone serum levels, such as calcitonin, PTH and vitamin D...
journal_title:Journal of endocrinological investigation
pub_type: 杂志文章,评审
doi:
更新日期:2003-01-01 00:00:00