Slug down-regulation by RNA interference inhibits invasion growth in human esophageal squamous cell carcinoma.


BACKGROUND:Esophageal squamous cell carcinoma (ESCC) is one of the most aggressive carcinomas of the gastrointestinal tract. We assessed the relevance of Slug in measuring the invasive potential of ESCC cells in vitro and in vivo in immunodeficient mice. METHODS:We utilized RNA interference to knockdown Slug gene expression, and effects on survival and invasive carcinoma were evaluated using a Boyden chamber transwell assay in vitro. We evaluated the effect of Slug siRNA-transfection and Slug cDNA-transfection on E-cadherin and Bcl-2 expression in ESCC cells. A pseudometastatic model of ESCC in immunodeficient mice was used to assess the effects of Slug siRNA transfection on tumor metastasis development. RESULTS:The EC109 cell line was transfected with Slug-siRNA to knockdown Slug expression. The TE13 cell line was transfected with Slug-cDNA to increase Slug expression. EC109 and TE13 cell lines were tested for the expression of apoptosis-related genes bcl-2 and metastasis-related gene E-cadherin identified previously as Slug targets. Bcl-2 expression was increased and E-cadherin was decreased in Slug siRNA-transfected EC109 cells. Bcl-2 expression was increased and E-cadherin was decreased in Slug cDNA-transfected TE13 cells. Invasion of Slug siRNA-transfected EC109 cells was reduced and apoptosis was increased whereas invasion was greater in Slug cDNA-transfected cells. Animals injected with Slug siRNA-transfected EC109 cells exhibited fewer seeded nodes and demonstrated more apoptosis. CONCLUSIONS:Slug down-regulation promotes cell apoptosis and decreases invasion capability in vitro and in vivo. Slug inhibition may represent a novel strategy for treatment of metastatic ESCC.


BMC Gastroenterol


BMC gastroenterology


Tang P,Yu Z,Zhang K,Wang Y,Ma Z,Zhang S,Chen D,Zhou Y




Has Abstract


2011-05-20 00:00:00










  • Risk of inflammatory bowel disease following a diagnosis of irritable bowel syndrome.

    abstract:BACKGROUND:Irritable bowel syndrome (IBS) and inflammatory bowel disease (IBD) symptoms often overlap. In some IBS cases there are subtle inflammatory changes similar to the immune-mediated pathophysiology of IBD, and the risk of both increases after infectious gastroenteritis (IGE). METHODS:To evaluate the effect of ...

    journal_title:BMC gastroenterology

    pub_type: 杂志文章


    authors: Porter CK,Cash BD,Pimentel M,Akinseye A,Riddle MS

    更新日期:2012-05-28 00:00:00

  • Possible etiology of improvements in both quality of life and overlapping gastroesophageal reflux disease by proton pump inhibitor treatment in a prospective randomized controlled trial.

    abstract:BACKGROUND:Symptoms suggestive of functional dyspepsia (FD) and irritable bowel syndrome (IBS) frequently overlap with those of gastroesophageal reflux disease. Despite the high prevalence of symptomatic overlap, the underlying etiology remains poorly defined. We assessed the correlation of symptomatic relief and healt...

    journal_title:BMC gastroenterology

    pub_type: 杂志文章,多中心研究,随机对照试验


    authors: Mönnikes H,Schwan T,van Rensburg C,Straszak A,Theek C,Lühmann R,Sander P,Tholen A

    更新日期:2013-10-01 00:00:00

  • Unsedated peroral wireless pH capsule placement vs. standard pH testing: a randomized study and cost analysis.

    abstract:BACKGROUND:Wireless capsule pH-metry (WC) is better tolerated than standard nasal pH catheter (SC), but endoscopic placement is expensive. AIMS:to confirm that non-endoscopic peroral manometric placement of WC is as effective and better tolerated than SC and to perform a cost analysis of the available esophageal pH-me...

    journal_title:BMC gastroenterology

    pub_type: 杂志文章,多中心研究,随机对照试验


    authors: Andrews CN,Sadowski DC,Lazarescu A,Williams C,Neshev E,Storr M,Au F,Heitman SJ

    更新日期:2012-05-31 00:00:00

  • Effect of antecolic or retrocolic reconstruction of the gastro/duodenojejunostomy on delayed gastric emptying after pancreaticoduodenectomy: a meta-analysis.

    abstract:BACKGROUND:Delayed gastric emptying (DGE) is one of the most frequent complications after pancreaticoduodenectomy (PD). This meta-analysis aimed to evaluate the effect of antecolic versus retrocolic reconstruction of gastro/duodenojejunostomy on DGE after PD. METHODS:Randomized controlled trials (RCTs) comparing antec...

    journal_title:BMC gastroenterology

    pub_type: 杂志文章,meta分析


    authors: Zhou Y,Lin J,Wu L,Li B,Li H

    更新日期:2015-06-16 00:00:00

  • Are proton pump inhibitors the first choice for acute treatment of gastric ulcers? A meta analysis of randomized clinical trials.

    abstract:BACKGROUND:Gastric ulcers are a frequent problem in the United States. Proton pump inhibitors have been shown to increase healing rates and improve clinical symptoms. The objective of this study is to compare gastric ulcer healing rates for patients treated with a proton pump inhibitor (PPI) (omeprazole, rabeprazole, p...

    journal_title:BMC gastroenterology

    pub_type: 杂志文章,meta分析


    authors: Salas M,Ward A,Caro J

    更新日期:2002-07-15 00:00:00

  • Helicobacter pylori infection progresses proximally associated with pyloric metaplasia in age-dependent tendency: a cross-sectional study.

    abstract:BACKGROUND:The elderly population presents higher morbidity of H. pylori associated diseases in proximal stomach. The specific pathogenesis and mechanism have not been clearly addressed. The gastric environment for H. pylori colonization is dynamic with increasing age. The aim of present study is to investigate the cor...

    journal_title:BMC gastroenterology

    pub_type: 杂志文章


    authors: Shi H,Xiong H,Qian W,Lin R

    更新日期:2018-10-29 00:00:00

  • Diagnosis of celiac disease and applicability of ESPGHAN guidelines in Mediterranean countries: a real life prospective study.

    abstract:BACKGROUND:We assessed how the diagnosis of Celiac Disease (CD) is made and how the new ESPGHAN guidelines can be applied in children from countries with different resources. METHODS:A real life prospective study was performed in 14 centres of 13 different Mediterranean countries. Participants were asked to apply the ...

    journal_title:BMC gastroenterology

    pub_type: 杂志文章


    authors: Smarrazzo A,Misak Z,Costa S,Mičetić-Turk D,Abu-Zekry M,Kansu A,Abkari A,Bouziane-Nedjadi K,Ben Hariz M,Roma E,Velmishi V,Legarda Tamara M,Attard T,Djurisic V,Greco L,Magazzù G

    更新日期:2017-01-21 00:00:00

  • Oral versus intravenous proton pump inhibitors in preventing re-bleeding for patients with peptic ulcer bleeding after successful endoscopic therapy.

    abstract:BACKGROUND:High dose intravenous proton pump inhibitor after endoscopic therapy for peptic ulcer bleeding has been recommended as adjuvant therapy. Whether oral proton pump inhibitor can replace intravenous proton pump inhibitor in this setting is unknown. This study aims to compare the clinical efficacy of oral and in...

    journal_title:BMC gastroenterology

    pub_type: 杂志文章,随机对照试验


    authors: Yen HH,Yang CW,Su WW,Soon MS,Wu SS,Lin HJ

    更新日期:2012-06-08 00:00:00

  • Liver disease and 30-day mortality after colorectal cancer surgery: a Danish population-based cohort study.

    abstract:BACKGROUND:Colorectal cancer (CRC) is common, with surgery as the main curative treatment. The prevalence of chronic liver disease has increased, but knowledge is limited on postoperative mortality in patients with liver disease who undergo CRC surgery. Hence, we examined 30-day mortality after CRC surgery in patients ...

    journal_title:BMC gastroenterology

    pub_type: 杂志文章


    authors: Montomoli J,Erichsen R,Christiansen CF,Ulrichsen SP,Pedersen L,Nilsson T,Sørensen HT

    更新日期:2013-04-15 00:00:00

  • 25(OH) D3 alleviate liver NK cytotoxicity in acute but not in chronic fibrosis model of BALB/c mice due to modulations in vitamin D receptor.

    abstract:BACKGROUND:Low 25-Hydroxy-vitamin-D; "25(OH)-D3" serum and vitamin D receptor (VDR) levels were recently correlated to advanced fibrosis. However, VDR mechanism in liver fibrosis modulations is not well understood. In this study, we aimed to evaluate changes in liver NK cells cytotoxicity due to modulations in VDR in C...

    journal_title:BMC gastroenterology

    pub_type: 杂志文章


    authors: Salhab A,Amer J,Yinying L,Safadi R

    更新日期:2020-04-10 00:00:00

  • Gall bladder wall thickening as non-invasive screening parameter for esophageal varices - a comparative endoscopic - sonographic study.

    abstract:BACKGROUND:The mortality due to hemorrhage of esophageal varices (EV) is still high. The predominant cause for EV is liver cirrhosis, which has a high prevalence in Western Europe. Therefore, non-invasive screening markers for the presence of EV are of interest. Here, we aim to investigate whether non-inflammatory gall...

    journal_title:BMC gastroenterology

    pub_type: 杂志文章


    authors: Tsaknakis B,Masri R,Amanzada A,Petzold G,Ellenrieder V,Neesse A,Kunsch S

    更新日期:2018-08-02 00:00:00

  • Trends in gastroenteritis-associated mortality in the United States, 1985-2005: variations by ICD-9 and ICD-10 codes.

    abstract:BACKGROUND:Trends in gastroenteritis-associated mortality are changing over time with development of antibiotic resistant strains of certain pathogens, improved diagnostic methods, and changing healthcare. In 1999, ICD-10 coding was introduced for mortality records which can also affect trends. We assess trends in gast...

    journal_title:BMC gastroenterology

    pub_type: 杂志文章


    authors: Jagai JS,Smith GS,Schmid JE,Wade TJ

    更新日期:2014-12-10 00:00:00

  • Indirect costs associated with ulcerative colitis: a systematic literature review of real-world data.

    abstract:BACKGROUND:The economic burden of ulcerative colitis (UC), specifically related to indirect costs, is not extensively documented. Understanding and quantifying it is required by health care decision makers. AIM:To assess the impact of indirect costs of UC in observation studies. METHOD:A systematic literature search ...

    journal_title:BMC gastroenterology

    pub_type: 杂志文章


    authors: Constantin J,Atanasov P,Wirth D,Borsi A

    更新日期:2019-11-09 00:00:00

  • Increasing rate of inflammatory bowel disease: a 12-year retrospective study in NingXia, China.

    abstract:BACKGROUND:In China, the incidence of Inflammatory bowel disease (IBD) has shown a significant growth trend. Analysis of the epidemiology, clinical manifestations, diagnostic means, and treatment of IBD will further improve the clinician's understanding of IBD, improve knowledge and further enable early diagnosis and s...

    journal_title:BMC gastroenterology

    pub_type: 杂志文章


    authors: Zhai H,Liu A,Huang W,Liu X,Feng S,Wu J,Yao Y,Wang C,Li Q,Hao Q,Hu J,Zhang S

    更新日期:2016-01-12 00:00:00

  • "Sarcopenia and intramuscular fat deposition are associated with poor survival in Indonesian patients with hepatocellular carcinoma: a retrospective study".

    abstract:BACKGROUND:A large-scale Japanese study showed that low skeletal muscle index (SMI) and intramuscular fat (IMF) deposition are associated with hepatocellular carcinoma (HCC) survival. Here, we evaluated the effects of SMI and IMF on the survival of Indonesian HCC patients, whose characteristics differ from those of Jap...

    journal_title:BMC gastroenterology

    pub_type: 杂志文章


    authors: Mardian Y,Yano Y,Ratnasari N,Choridah L,Wasityastuti W,Setyawan NH,Hayashi Y

    更新日期:2019-12-30 00:00:00

  • Gynecomastia during imatinib mesylate treatment for gastrointestinal stromal tumor: a rare adverse event.

    abstract:BACKGROUND:Imatinib mesylate has been the standard therapeutic treatment for chronic myeloid leukemia, advanced and metastatic gastrointestinal stromal tumor (GIST). It is well tolerated with mild adverse effects. Gynecomastia development during the course of treatment has been rarely reported. METHODS:Ninety-eight pa...

    journal_title:BMC gastroenterology

    pub_type: 杂志文章


    authors: Liu H,Liao G,Yan Z

    更新日期:2011-11-02 00:00:00

  • Genetic factors associated with intestinal metaplasia in a high risk Singapore-Chinese population: a cohort study.

    abstract:BACKGROUND:Intestinal metaplasia (IM) is an important precursor lesion in the development of gastric cancer (GC). The aim of this study was to investigate genetic factors previously linked to GC risk for their possible association with IM. A total of 18 polymorphisms in 14 candidate genes were evaluated in a Singapore-...

    journal_title:BMC gastroenterology

    pub_type: 杂志文章


    authors: Zhu F,Loh M,Hill J,Lee S,Koh KX,Lai KW,Salto-Tellez M,Iacopetta B,Yeoh KG,Soong R,Singapore Gastric Cancer Consortium.

    更新日期:2009-10-13 00:00:00

  • Intraductal papillary mucinous carcinoma of the pancreas associated with pancreas divisum: a case report and review of the literature.

    abstract:BACKGROUND:Pancreas divisum, the most common congenital anomaly of the pancreas, is caused by failure of the fusion of the ventral and dorsal pancreatic duct systems during embryological development. Although various pancreatic tumors can occur in patients with pancreas divisum, intraductal papillary mucinous neoplasm ...

    journal_title:BMC gastroenterology

    pub_type: 杂志文章,评审


    authors: Nishi T,Kawabata Y,Ishikawa N,Araki A,Yano S,Maruyama R,Tajima Y

    更新日期:2015-07-08 00:00:00

  • Macro-AST: misleading finding in an adolescent with MCAD-deficiency.

    abstract:BACKGROUND:MCAD-deficiency is the most common inborn error of fatty acid oxidation now included in many newborn screening programms using MS/MS. During prolonged catabolic episodes, patients may suffer from metabolic decompensation with dysfunction of liver, skeletal- and heart muscle as well as brain. In anabolism, ne...

    journal_title:BMC gastroenterology

    pub_type: 杂志文章


    authors: Das AM,Drache S,Janzen N,Franke A

    更新日期:2012-08-30 00:00:00

  • A case report of prostate cancer metastasis to the stomach resembling undifferentiated-type early gastric cancer.

    abstract:BACKGROUND:Occurrence of metastatic cancer to the stomach is rare, particularly in patients with prostate cancer. Gastric metastasis generally presents as a solitary and submucosal lesion with a central depression. CASE PRESENTATION:We describe a case of gastric metastasis arising from prostate cancer, which is almost...

    journal_title:BMC gastroenterology

    pub_type: 杂志文章


    authors: Inagaki C,Suzuki T,Kitagawa Y,Hara T,Yamaguchi T

    更新日期:2017-08-07 00:00:00

  • Influence of cirrhosis on long-term prognosis after surgery in patients with combined hepatocellular-cholangiocarcinoma.

    abstract:BACKGROUND:Little is known about the prognostic impact of cirrhosis on long-term survival of patients with combined hepatocellular-cholangiocarcinoma (cHCC-CC) after hepatic resection. The aim of this study was to elucidate the long-term outcome of hepatectomy in cHCC-CC patients with cirrhosis. METHODS:A total of 144...

    journal_title:BMC gastroenterology

    pub_type: 杂志文章


    authors: Zhou YM,Sui CJ,Zhang XF,Li B,Yang JM

    更新日期:2017-02-10 00:00:00

  • Long intestinal tube splinting prevents postoperative adhesive small-bowel obstruction in sclerosing encapsulating peritonitis.

    abstract:BACKGROUND:Sclerosing encapsulating peritonitis (SEP) is a rare cause of small-bowel obstruction. The optimal treatment for this condition remains controversial. METHODS:In this study, we performed a retrospective analysis of the data of 44 patients who underwent surgery for SEP between December 2001 and 2008 at our h...

    journal_title:BMC gastroenterology

    pub_type: 杂志文章


    authors: Li M,Zhu W,Li Y,Jiang J,Li J,Li N

    更新日期:2014-11-25 00:00:00

  • Imbalanced mucosal microcirculation in the remission stage of ulcerative colitis using probe-based confocal laser endomicroscopy.

    abstract:BACKGROUND:Microcirculatory disturbance is an important factor in the pathogenesis of Inflammatory Bowel Disease (IBD) but there have been few studies in this field. Confocal Laser Endomicroscopy (CLE) has been used over the last 10 years and has made it possible to explore the changes in microcirculation of the coloni...

    journal_title:BMC gastroenterology

    pub_type: 杂志文章


    authors: Tian Y,Zheng Y,Teng G,Li J,Wang H

    更新日期:2019-07-01 00:00:00

  • Gene expression analysis of a Helicobacter pylori-infected and high-salt diet-treated mouse gastric tumor model: identification of CD177 as a novel prognostic factor in patients with gastric cancer.

    abstract:BACKGROUND:Helicobacter pylori (H. pylori) infection and excessive salt intake are known as important risk factors for stomach cancer in humans. However, interactions of these two factors with gene expression profiles during gastric carcinogenesis remain unclear. In the present study, we investigated the global gene ex...

    journal_title:BMC gastroenterology

    pub_type: 杂志文章


    authors: Toyoda T,Tsukamoto T,Yamamoto M,Ban H,Saito N,Takasu S,Shi L,Saito A,Ito S,Yamamura Y,Nishikawa A,Ogawa K,Tanaka T,Tatematsu M

    更新日期:2013-07-30 00:00:00

  • Implementation of population screening for colorectal cancer by repeated Fecal Immunochemical Test (FIT): third round.

    abstract:BACKGROUND:Colorectal cancer (CRC) is the most common cancer in Europe with a mortality rate of almost 50%. The prognosis of patients is largely determined by the clinical and pathological stage at the time of diagnosis. Population screening has been shown to reduce CRC-related mortality rate. Most screening programs w...

    journal_title:BMC gastroenterology

    pub_type: 杂志文章


    authors: Stegeman I,de Wijkerslooth TR,Mallant-Hent RC,de Groot K,Stroobants AK,Fockens P,Mundt M,Bossuyt PM,Dekker E

    更新日期:2012-06-19 00:00:00

  • Proteomics analysis of serum protein profiling in pancreatic cancer patients by DIGE: up-regulation of mannose-binding lectin 2 and myosin light chain kinase 2.

    abstract:BACKGROUND:Pancreatic cancer has significant morbidity and mortality worldwide. Good prognosis relies on an early diagnosis. The purpose of this study was to develop techniques for identifying cancer biomarkers in the serum of patients with pancreatic cancer. METHODS:Serum samples from five individuals with pancreatic...

    journal_title:BMC gastroenterology

    pub_type: 杂志文章


    authors: Rong Y,Jin D,Hou C,Hu J,Wu W,Ni X,Wang D,Lou W

    更新日期:2010-06-29 00:00:00

  • Narrow-band imaging and high-definition white-light endoscopy in patients with serrated lesions not fulfilling criteria for serrated polyposis syndrome: a randomized controlled trial with tandem colonoscopy.

    abstract:BACKGROUND:It is unknown whether narrow-band imaging (NBI) could be more effective than high-definition white-light endoscopy (HD-WLE) in detecting serrated lesions in patients with prior serrated lesions > 5 mm not completely fulfilling serrated polyposis syndrome (SPS) criteria. METHODS:We conducted a randomized, cr...

    journal_title:BMC gastroenterology

    pub_type: 杂志文章,随机对照试验


    authors: Riu Pons F,Andreu M,Naranjo D,Álvarez-González MA,Seoane A,Dedeu JM,Barranco L,Bessa X

    更新日期:2020-04-16 00:00:00

  • Transarterial chemoembolization plus sorafenib for the management of unresectable hepatocellular carcinoma: a systematic review and meta-analysis.

    abstract:BACKGROUND:Transarterial chemoembolization (TACE) is the recommended treatment for hepatocellular carcinoma (HCC) patients at Barcelona Clinic Liver Cancer (BCLC) B-stage, whereas sorafenib is an orally administered small molecule target drug for BCLC C-stage. This updated systemic review and meta-analysis focuses on i...

    journal_title:BMC gastroenterology

    pub_type: 杂志文章,meta分析,评审


    authors: Li L,Zhao W,Wang M,Hu J,Wang E,Zhao Y,Liu L

    更新日期:2018-09-04 00:00:00

  • Case report: an aortic aneurysm as cause of pseudoachalasia.

    abstract:BACKGROUND:Pseudoachalasia is a rare disorder which has clinical, radiographic, and manometric findings that are often indistinguishable from primary achalasia. It is usually associated with malignancy. Few reports describe vascular compression as a cause of pseudoachalasia. CASE PRESENTATION:Here we present a case of...

    journal_title:BMC gastroenterology

    pub_type: 杂志文章


    authors: Dejaeger M,Lormans M,Dejaeger E,Fagard K

    更新日期:2020-03-06 00:00:00

  • The association of hepatitis c virus infection status with serum glucose levels.

    abstract:BACKGROUND:Hepatitis C virus (HCV) infection is commonly associated with a disturbance of glucose metabolism. However, there have been conflicting reports on whether the clearance of the HCV may be followed by changes of serum blood glucose and insulin resistance. The aim of the present study was to evaluate the impact...

    journal_title:BMC gastroenterology

    pub_type: 杂志文章


    authors: Li Y,Wang X,Yu G,Sun H,Lv J,Chi X,Wu R,Gao X,Niu J

    更新日期:2019-06-13 00:00:00