Modifications of oxido-reductase activities in adriamycin-resistant leukaemia K562 cells.

Abstract:

:Adriamycin (ADR), a well-known antitumoral drug, interacts with DNA (nuclear and mitochondrial) and cardiolipin. Moreover, ADR induces numerous mitochondrial modifications in sensitive cells. However, no results have yet been obtained as to the repercussions of drug effects on oxido-reductase activities in ADR-resistant cells. To analyze mitochondrial damage induced by ADR treatment, we investigated lactate content, oxygen consumption, respiratory chain activities, and cytochrome content in ADR-sensitive K562 cells and two ADR-resistant variants (K562/R0.2 and K562/R0.5 cells). Biochemical investigations in ADR-resistant cells showed several mitochondrial modifications (in comparison to the parental cell line) according to the variant line and the physiologic state. More particularly, in K562/R0.5 cells cytochrome c (cyt c) oxidase (COX; EC 1.9.3.1) activity and cytochrome aa3 content dramatically decreased since cells enter into the stationary phase. Regardless of the number of multidrug-resistant cell subcultures in ADR-free medium, the cytochrome c oxidase activity in the stationary phase remained unchanged, indicating an irreversible effect of the drug. These alterations could correspond to several modifications of the nuclear and/or mitochondrial genome(s) following acquisition of the ADR resistance phenotype by K562 cells.

journal_name

Biochem Pharmacol

journal_title

Biochemical pharmacology

authors

Denis-Gay M,Petit JM,Mazat JP,Ratinaud MH

doi

10.1016/s0006-2952(98)00084-7

subject

Has Abstract

pub_date

1998-08-15 00:00:00

pages

451-7

issue

4

eissn

0006-2952

issn

1873-2968

pii

S0006-2952(98)00084-7

journal_volume

56

pub_type

杂志文章
  • Nicotinic ligands as multifunctional agents for the treatment of neuropsychiatric disorders.

    abstract::The challenges associated with developing more effective treatments for neurologic and psychiatric illness such as Alzheimer's disease and schizophrenia are considerable. Both the symptoms and the pathophysiology of these conditions are complex and poorly understood and the clinical presentations across different pati...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章,评审

    doi:10.1016/j.bcp.2015.07.027

    authors: Terry AV Jr,Callahan PM,Hernandez CM

    更新日期:2015-10-15 00:00:00

  • Menadione: spectrum of anticancer activity and effects on nucleotide metabolism in human neoplastic cell lines.

    abstract::The spectrum of cytotoxicity of menadione (MD) was examined in a panel of human cancer cell lines. MD was equipotent against multidrug-resistant and parental leukemia cell lines with IC50 values of 13.5 +/- 3.6 and 18 +/- 2.4 microM respectively. A cervical carcinoma cell line resistant to the antimetabolite, methotre...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章

    doi:10.1016/0006-2952(91)90099-q

    authors: Nutter LM,Cheng AL,Hung HL,Hsieh RK,Ngo EO,Liu TW

    更新日期:1991-05-01 00:00:00

  • Nitric oxide-independent suppression of P450 2C11 expression by interleukin-1beta and endotoxin in primary rat hepatocytes.

    abstract::Hepatic expression of multiple cytochrome P450 genes is suppressed in the livers of rats undergoing an inflammatory response. Nitric oxide (NO) released during inflammation has been implicated in the decreased activities and expression of several cytochrome P450 isozymes. We examined the role of cytokine-mediated NO r...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章

    doi:10.1016/s0006-2952(97)00226-8

    authors: Sewer MB,Morgan ET

    更新日期:1997-09-15 00:00:00

  • Clavilactones, a novel class of tyrosine kinase inhibitors of fungal origin.

    abstract::Targeting of deregulated protein tyrosine kinases has been proposed as a new approach in the therapeutic intervention against pathological processes including proliferative disorders and cancer. Using a screening approach based on a comparative evaluation of antiproliferative effects in a panel of tumor cells with dif...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章

    doi:10.1016/s0006-2952(00)00278-1

    authors: Cassinelli G,Lanzi C,Pensa T,Gambetta RA,Nasini G,Cuccuru G,Cassinis M,Pratesi G,Polizzi D,Tortoreto M,Zunino F

    更新日期:2000-06-15 00:00:00

  • Pharmacokinetics relevant to the anti-carcinogenic and anti-tumor activities of glucarate and the synergistic combination of glucarate:retinoid in the rat.

    abstract::Alone and in synergistic combination with retinoids, dietary glucarate inhibits both the chemical induction and growth of rat mammary tumors. To investigate the pharmacokinetics of glucarate, [14C]glucarate was synthesized, converted to the calcium salt, and administered to rats bearing primary mammary tumors. When gi...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章

    doi:10.1016/0006-2952(94)90544-4

    authors: Webb TE,Pham-Nguyen MH,Darby M,Hamme AT 2nd

    更新日期:1994-04-29 00:00:00

  • Enhancement of the functional stability of solubilized nucleoside transporters by substrates and inhibitors.

    abstract::Purification of functional nucleoside transporters has been hampered by the instability of detergent-solubilized proteins. The present study was undertaken to determine if the presence of specific transporter ligands in the solubilization medium could enhance the functional stability of the isolated proteins. Ehrlich ...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章

    doi:10.1016/s0006-2952(96)00857-x

    authors: Hammond JR

    更新日期:1997-03-07 00:00:00

  • Lipids, LXRs and prostate cancer: are HDACs a new link?

    abstract::Lipids play a complex role in prostate cancer (PCa). Increased de novo synthesis of fatty acids and/or cholesterol is associated with the development of prostate tumors. Liver X Receptors (LXRs) are members of the nuclear receptor family that regulates intracellular lipid homeostasis. Targeting the transcriptional act...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章,评审

    doi:10.1016/j.bcp.2013.04.005

    authors: Hoang JJ,Baron S,Volle DH,Lobaccaro JM,Trousson A

    更新日期:2013-07-01 00:00:00

  • Selective inhibition of human mast cell tryptase by gabexate mesylate, an antiproteinase drug.

    abstract::Gabexate mesylate is a non-antigenic synthetic inhibitor of trypsin-like serine proteinases that is therapeutically used in the treatment of pancreatitis and disseminated intravascular coagulation and as a regional anticoagulant for hemodialysis. Considering the structural similarity between gabexate mesylate and argi...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章

    doi:10.1016/s0006-2952(00)00550-5

    authors: Erba F,Fiorucci L,Pascarella S,Menegatti E,Ascenzi P,Ascoli F

    更新日期:2001-02-01 00:00:00

  • Rat CYP24A1 acts on 20-hydroxyvitamin D(3) producing hydroxylated products with increased biological activity.

    abstract::20-Hydroxyvitamin D(3) (20(OH)D(3)), the major product of CYP11A1 action on vitamin D(3), is biologically active and is produced in vivo. As well as potentially having important physiological actions, it is of interest as a therapeutic agent due to its lack of calcemic activity. In the current study we have examined t...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章

    doi:10.1016/j.bcp.2012.09.032

    authors: Tieu EW,Tang EK,Chen J,Li W,Nguyen MN,Janjetovic Z,Slominski A,Tuckey RC

    更新日期:2012-12-15 00:00:00

  • Novel modified adenosine 5'-triphosphate analogues pharmacologically characterized in human embryonic kidney 293 cells highly expressing rat brain P2Y(1) receptor: Biotinylated analogue potentially suitable for specific P2Y(1) receptor isolation.

    abstract::Rat brain P2Y(1) (rP2Y(1)) receptor-transfected human embryonic kidney cells (HEK 293) were recently shown to have enhanced reactivity to both ATP and ADP (Vöhringer C, Schäfer R, Reiser G. Biochem Pharmacol 2000;59:791-800). Here, we demonstrated the usefulness of this cell line as a system for further studying novel...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章

    doi:10.1016/s0006-2952(01)00593-7

    authors: Zündorf G,Schäfer R,Vöhringer C,Halbfinger E,Fischer B,Reiser G

    更新日期:2001-05-15 00:00:00

  • Metabolism of diethylstilbestrol in hamster hepatocytes.

    abstract::Under certain modulating conditions the liver of the male Syrian golden hamster is a target organ for the carcinogenic effect of the synthetic estrogen diethylstilbestrol (DES). As a basis for mechanistic studies aimed at elucidating the role of metabolic activation in the process of DES-induced neoplasia, the metabol...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章

    doi:10.1016/0006-2952(87)90623-x

    authors: Blaich G,Pfaff E,Metzler M

    更新日期:1987-10-01 00:00:00

  • Evidence for involvement of tyrosine phosphorylation in taxol-induced apoptosis in a human ovarian tumor cell line.

    abstract::Taxol is an antineoplastic agent with significant activity against ovarian as well as breast cancer. To investigate mechanisms by which taxol exerts its cytotoxic action, taxol-induced apoptosis, characterized by morphologic changes and internucleosomal DNA fragmentation, was examined in a human ovarian tumor cell lin...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章

    doi:10.1016/0006-2952(94)90164-3

    authors: Liu Y,Bhalla K,Hill C,Priest DG

    更新日期:1994-09-15 00:00:00

  • Effects of calcium depletion on norepinephrine- and A23187-induced stimulation of inositol phosphate formation.

    abstract::The role of calcium in the stimulation of phosphoinositide (PIn) hydrolysis by norepinephrine and the calcium ionophore A23187 was investigated in chopped cerebral cortex in which the PIns had been labeled previously with ([3H]myo-inositol. The accumulation of the newly formed [3H]inositol phosphate ([3H]IPs) was used...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章

    doi:10.1016/0006-2952(87)90222-x

    authors: Knepper SM,Rutledge CO

    更新日期:1987-09-15 00:00:00

  • Biological activity and molecular interaction of a netropsin-acridine hybrid ligand with chromatin and topoisomerase II.

    abstract::A hybrid molecule, which combines an anilinoacridine chromophore related to the antitumour drug amsacrine (m-AMSA) and a bispyrrole moiety analogous to the antiviral agent netropsin, has been examined for its ability to bind chromatin and to modulate the activity of topoisomerase II. The results show that the presence...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章

    doi:10.1016/0006-2952(92)90564-y

    authors: Bailly C,Collyn-d'Hooghe M,Lantoine D,Fournier C,Hecquet B,Fosse P,Saucier JM,Colson P,Houssier C,Hénichart JP

    更新日期:1992-02-04 00:00:00

  • DIF-1 inhibits tumor growth in vivo reducing phosphorylation of GSK-3β and expressions of cyclin D1 and TCF7L2 in cancer model mice.

    abstract::We reported that differentiation-inducing factor-1 (DIF-1), synthesized by Dictyostelium discoideum, inhibited proliferation of various tumor cell lines in vitro by suppressing the Wnt/β-catenin signaling pathway. However, it remained unexplored whether DIF-1 also inhibits tumor growth in vivo. In the present study, t...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章

    doi:10.1016/j.bcp.2014.03.006

    authors: Takahashi-Yanaga F,Yoshihara T,Jingushi K,Igawa K,Tomooka K,Watanabe Y,Morimoto S,Nakatsu Y,Tsuzuki T,Nakabeppu Y,Sasaguri T

    更新日期:2014-06-01 00:00:00

  • Clinical translation of liver regeneration therapies: A conceptual road map.

    abstract::The increasing incidence of severe liver diseases worldwide has resulted in a high demand for curative liver transplantation. Unfortunately, the need for transplants by far eclipses the availability of suitable grafts leaving many waitlisted patients to face liver failure and often death. Routine use of smaller grafts...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章,评审

    doi:10.1016/j.bcp.2020.113847

    authors: Greenbaum LE,Ukomadu C,Tchorz JS

    更新日期:2020-05-01 00:00:00

  • Prostaglandin synthetase catalyzed activation of paracetamol.

    abstract::Prostaglandin synthetase has the ability to catalyze the metabolism of paracetamol to a reactive metabolite, which binds to protein and reduced glutathione (GSH). This was demonstrated with microsomes isolated from both sheep seminal vesicles (SSV) and rabbit kidney medulla. The activation of paracetamol occurred thro...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章

    doi:10.1016/0006-2952(82)90029-6

    authors: Moldéus P,Andersson B,Rahimtula A,Berggren M

    更新日期:1982-04-01 00:00:00

  • Acute and chronic in vivo therapeutic resistance.

    abstract::The response and engagement of host normal tissues in malignant disease are major factors in therapeutic resistance. Physically, solid tumors have regions of hypoxia and acidosis. These physical stresses can lead to a more aggressive malignant phenotype through activation of HIF, GLUT-1, carbonic anhydrase IX, and sub...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章,评审

    doi:10.1016/j.bcp.2009.01.006

    authors: Teicher BA

    更新日期:2009-06-01 00:00:00

  • Binding of [3H]perhydrohistrionicotoxin and [3H]phencyclidine to the nicotinic receptor-ion channel complex of Torpedo electroplax. Inhibition by histrionicotoxins and derivatives.

    abstract::Histrionicotoxin, a spiropiperidine alkaloid, and twenty-two analogs inhibited binding of [3H]perhydrohistrionicotoxin [( 3H]H12-HTX) and of [3H]phencyclidine [( 3H]PCP) to sites on the acetylcholine receptor-ion complex of Torpedo electroplax membranes. Structural alterations to the nitrogen (secondary amine) or oxyg...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章

    doi:10.1016/0006-2952(85)90145-5

    authors: Aronstam RS,King CT Jr,Albuquerque EX,Daly JW,Feigl DM

    更新日期:1985-09-01 00:00:00

  • Serotonin glucuronidation by Ah receptor- and oxidative stress-inducible human UDP-glucuronosyltransferase (UGT) 1A6 in Caco-2 cells.

    abstract::Caco-2 cells are a widely used model in drug development to study intestinal drug transport and metabolism. Recently, serotonin (5-hydroxytryptamine, 5-HT) has been characterized as a highly selective substrate of human UDP-glucuronosyltransferase UGT1A6 [Krishnaswamy S, Duan SX, von Moltke LL, Greenblatt DJ, Court MH...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章

    doi:10.1016/j.bcp.2005.02.010

    authors: Köhle C,Badary OA,Nill K,Bock-Hennig BS,Bock KW

    更新日期:2005-05-01 00:00:00

  • Degradation of endomorphin-2 at the supraspinal level in mice is initiated by dipeptidyl peptidase IV: an in vitro and in vivo study.

    abstract::Endomorphin-2 (Tyr-Pro-Phe-PheNH(2)) was discovered as an endogenous ligand for the mu-opioid receptor. The physiological function of endomorphin-2 as a neurotransmitter or neuromodulator may cease through the rapid enzymatic process in the synapse of brain, as for other neuropeptides. The present study was conducted ...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章

    doi:10.1016/s0006-2952(03)00391-5

    authors: Sakurada C,Sakurada S,Hayashi T,Katsuyama S,Tan-No K,Sakurada T

    更新日期:2003-08-15 00:00:00

  • Distamycin inhibits the binding of a nuclear factor to the -278/-256 upstream sequence of the human HLA-DR alpha gene.

    abstract::In this study we analyse the effects of the anti-tumor compound distamycin on the binding of nuclear factor(s) to a synthetic oligonucleotide (GTATA/IFN-gamma) mimicking a putative regulatory region of the human HLA-DR alpha gene. This region contains the sequence (GTATA), that is required for nuclear protein binding ...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章

    doi:10.1016/0006-2952(91)90620-k

    authors: Gambari R,Barbieri R,Nastruzzi C,Chiorboli V,Feriotto G,Natali PG,Giacomini P,Arcamone F

    更新日期:1991-02-15 00:00:00

  • Pharmacological characterization of novel adenosine ligands in recombinant and native human A2B receptors.

    abstract::The present study was designed to evaluate the effects of novel and recognised compounds at human recombinant A(2B) adenosine receptors expressed in Chinese hamster ovary (hA(2B)CHO), in human embryonic kidney 293 (hA(2B)HEK-293) and at endogenous A(2B) receptors in human mast cells (HMC-1). Saturation binding experim...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章

    doi:10.1016/j.bcp.2005.08.018

    authors: Varani K,Gessi S,Merighi S,Vincenzi F,Cattabriga E,Benini A,Klotz KN,Baraldi PG,Tabrizi MA,Lennan SM,Leung E,Borea PA

    更新日期:2005-11-25 00:00:00

  • Molecular characterization of EP6--a novel imidazo[1,2-a]pyridine based direct 5-lipoxygenase inhibitor.

    abstract::5-Lipoxygenase (5-LO) is a crucial enzyme of the arachidonic acid (AA) cascade and catalyzes the formation of bioactive leukotrienes (LTs) which are involved in inflammatory diseases and allergic reactions. The pathophysiological effects of LTs are considered to be prevented by 5-LO inhibitors. In this study we presen...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章

    doi:10.1016/j.bcp.2011.10.012

    authors: Wisniewska JM,Rödl CB,Kahnt AS,Buscató El,Ulrich S,Tanrikulu Y,Achenbach J,Rörsch F,Grösch S,Schneider G,Cinatl J Jr,Proschak E,Steinhilber D,Hofmann B

    更新日期:2012-01-15 00:00:00

  • Subcellular localization of the camptothecin analogues, topotecan and gimatecan.

    abstract::Lipophilicity of camptothecins derivatives has been reported to improve the stability of the lactone ring and to favor rapid uptake and intracellular accumulation. Recently, a novel series of lipophilic camptothecins substituted at position 7 was developed, and gimatecan (ST1481) was selected for clinical development ...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章

    doi:10.1016/j.bcp.2003.10.034

    authors: Croce AC,Bottiroli G,Supino R,Favini E,Zuco V,Zunino F

    更新日期:2004-03-15 00:00:00

  • Down-regulation of the HGF/MET autocrine loop induced by celecoxib and mediated by P-gp in MDR-positive human hepatocellular carcinoma cell line.

    abstract::Many tumors are resistant to drug-induced cell-cycle arrest and apoptosis. We have reported that apoptosis can be restored in human multidrug-resistant (MDR) hepatocellular carcinoma cell lines by celecoxib. Here we show that P-glycoprotein (P-gp) mediates cell-cycle arrest and autophagy induced by celecoxib in human ...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章

    doi:10.1016/j.bcp.2009.03.013

    authors: Mazzanti R,Platini F,Bottini C,Fantappiè O,Solazzo M,Tessitore L

    更新日期:2009-07-01 00:00:00

  • Dietary compounds prevent oxidative damage and nitric oxide production by cells involved in demyelinating disease.

    abstract::Oligodendrocytes and activated macrophages are involved in the immunopathology of demyelinating disease. In this study, we investigated the in vitro effect of dietary compounds, in particular flavonoids, on oxidative damage in OLN-93 oligodendrocytes and on nitric oxide (NO) production by NR8383 macrophages. Using a c...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章

    doi:10.1016/j.bcp.2003.10.018

    authors: van Meeteren ME,Hendriks JJ,Dijkstra CD,van Tol EA

    更新日期:2004-03-01 00:00:00

  • Indomethacin-induced renal damage: role of oxygen free radicals.

    abstract::Nonsteroidal anti-inflammatory drugs are used extensively in clinical medicine. In spite of their therapeutic utility, however, they are known to cause significant gastrointestinal and renal toxicities, circumstances that limit their use. The side effects produced in these organs have been attributed mainly to the inh...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章

    doi:10.1016/j.bcp.2003.09.023

    authors: Basivireddy J,Jacob M,Pulimood AB,Balasubramanian KA

    更新日期:2004-02-01 00:00:00

  • Neratinib decreases pro-survival responses of [sorafenib + vorinostat] in pancreatic cancer.

    abstract::The combination of the multi-kinase and chaperone inhibitor sorafenib and the histone deacetylase inhibitor vorinostat in pancreatic cancer patients has proven to be a safe and efficacious modality (NCT02349867). We determined the evolutionary mechanisms by with pancreatic tumors become resistant to [sorafenib + vorin...

    journal_title:Biochemical pharmacology

    pub_type: 临床试验,杂志文章

    doi:10.1016/j.bcp.2020.114067

    authors: Booth L,Poklepovic A,Dent P

    更新日期:2020-08-01 00:00:00

  • Insight into tartrate inhibition patterns in vitro and in vivo based on cocrystal structure with UDP-glucuronosyltransferase 2B15.

    abstract::Glucuronidation, catalyzed by UDP-glucuronosyltransferases (UGTs), is a crucial substance metabolism and elimination process that mostly occurs in the liver to protect the body from toxic substances and maintain homeostasis. The reaction functions well in a uridine diphosphate glucuronic acid (UDPGA) -dependent manner...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章

    doi:10.1016/j.bcp.2019.113753

    authors: Zhang L,Zhu L,Qu W,Wu F,Hu M,Xie W,Liu Z,Wang C

    更新日期:2020-02-01 00:00:00