Investigations on the in vitro metacyclogenesis of a visceral and a cutaneous human strain of Leishmania infantum.

Abstract:

:The in vitro metacyclogenesis of a visceral (VL) and cutaneous (CL) human strain of Leishmania infantum was monitored in order to find out the kinetics of this process and the in vitro infective capacity for macrophages of the metacyclic promastigotes developed. To identify, enumerate, and separate the metacyclic population, the complement-dependent lysis by normal serum and the agglutination by peanut agglutinin (PNA) were used, as they were shown to be useful for the purpose of this study. Maximum percentage of metacyclics was detected by both techniques on the 4th day of growth for VL and the 6th day for CL, and was higher for the VL strain. The in vitro infectivity for macrophages of two strains was assayed, and the high parasitization data obtained were transformed in order to determine the increase of the parasite burden for macrophages throughout the incubation time of the experiments (2-72 h post-infection (p.i.)). This parameter is denominated the infectivity ratio (%I) and calculated as follows: (number of intracellular parasites per infected macrophage at 'x' time p.i./number of intracellular parasites per infected macrophage at 2 h p.i.) x 100. When %I was calculated for promastigotes unagglutinated by PNA (PNA-)--metacyclic or infective promastigotes--at any time of culture, the %I at 72 h p.i. was always much higher than for agglutinated promastigotes (2.1-12.5 times)--non-infective promastigotes--and unfractionated promastigotes from culture (1.7-9.5 times), especially with VL parasites. Likewise, the %I for VL PNA- promastigotes from the 4th day of culture was 1.9 times higher than for CL PNA- promastigotes from the 6th day of culture. The higher resistance to lysis by serum, percentage of metacyclics (PNA-), and infectivity ratio of VL than CL could be related to a higher spreading capability into the host body associated with higher pathogenic effects of the visceral strain than the cutaneous one.

journal_name

Acta Trop

journal_title

Acta tropica

authors

Louassini M,Adroher FJ,Foulquié MR,Benítez R

doi

10.1016/s0001-706x(98)00041-2

subject

Has Abstract

pub_date

1998-07-30 00:00:00

pages

355-68

issue

3

eissn

0001-706X

issn

1873-6254

pii

S0001706X98000412

journal_volume

70

pub_type

杂志文章
  • The diagnosis and management of fever at household level in the Greater Accra Region, Ghana.

    abstract::> Qualitative research methods were used in rural and urban areas of the Greater Accra Region to generate data to describe the folk diagnosis, etiology and management of malaria. Respondents defined as fever a set of symptoms loosely concordant with clinical malaria. Primary cause of fever as heat and particularly in ...

    journal_title:Acta tropica

    pub_type: 杂志文章

    doi:10.1016/0001-706x(94)90025-6

    authors: Agyepong IA,Manderson L

    更新日期:1994-12-01 00:00:00

  • Triatoma infestans relies on salivary lysophosphatidylcholine to enhance Trypanosoma cruzi transmission.

    abstract::Triatoma infestans is a mandatory haematophagous vector of Chagas disease in Brazil. Despite a large number of studies on the anti-haemostatic molecules present in its saliva, the role of its salivary components on parasite transmission is poorly understood. Here, we show that the bioactive lipid molecule, lysophospha...

    journal_title:Acta tropica

    pub_type: 杂志文章

    doi:10.1016/j.actatropica.2017.10.022

    authors: Lima MS,Carneiro AB,Souto-Padron T,Jurberg J,Silva-Neto MAC,Atella GC

    更新日期:2018-02-01 00:00:00

  • Immune correlate study on human Schistosoma japonicum in a well-defined population in Leyte, Philippines: II. Cellular immune responses to S. japonicum recombinant and native antigens.

    abstract::Cellular immune responses to specific Schistosoma japonicum recombinant and native antigens were investigated in a defined study population of 155 individuals in the Philippines, where data collected from a 3-year observation of exposure, infection and reinfection pattern were used to categorically classify putative '...

    journal_title:Acta tropica

    pub_type: 杂志文章

    doi:10.1016/s0001-706x(02)00177-8

    authors: Acosta LP,Waine G,Aligui GD,Tiu WU,Olveda RM,McManus DP

    更新日期:2002-11-01 00:00:00

  • Closing in on 'perhaps the most dreadful of the remaining plagues': an independent view of the multidisciplinary alliance to optimize schistosomiasis control in Africa.

    abstract::The 4-year (2006-2010) alliance to optimize schistosomiasis control and transmission surveillance in sub-Saharan Africa (CONTRAST), funded by the European Commission, stands out as a 'game-changer' by successfully harnessing and combining new as well as previously available capabilities for schistosomiasis control in ...

    journal_title:Acta tropica

    pub_type: 杂志文章,评审

    doi:10.1016/j.actatropica.2013.08.016

    authors: Bergquist R

    更新日期:2013-11-01 00:00:00

  • Beyond the 'back yard': Lay knowledge about Aedes aegypti in northern Australia and its implications for policy and practice.

    abstract::Controlling dengue fever in Australia and internationally, relies heavily upon the actions of residents as well as community education and awareness of the risks. Although it has been well established in medical anthropology that the success of health interventions is highly dependent upon a clear grasp of lay knowled...

    journal_title:Acta tropica

    pub_type: 杂志文章

    doi:10.1016/j.actatropica.2010.05.012

    authors: McNaughton D,Clough A,Johnson P,Ritchie S,O'Neill S

    更新日期:2010-10-01 00:00:00

  • The Impact of Sociological and Environmental Factors for Dengue Infection in Kuala Lumpur, Malaysia.

    abstract:BACKGROUND:Dengue incidence has grown dramatically around the world in recent years. Vector control is the only method to reduce dengue incidence due to the lack of a vaccine available. By understanding the factors contributed to the vector densities such as environmental and sociological factors, dengue prevention and...

    journal_title:Acta tropica

    pub_type: 杂志文章

    doi:10.1016/j.actatropica.2021.105834

    authors: Adnan RA,Ramli MF,Othman HF,Asha'ri ZH,Ismail SNS,Samsudin S

    更新日期:2021-01-21 00:00:00

  • Daily activity patterns of movement and refuge use in Triatoma gerstaeckeri and Rhodnius prolixus (Hemiptera: Reduviidae), vectors of the Chagas disease parasite.

    abstract::Transmission risk for vector borne disease is greatest during periods of heightened vector activity. Triatomine bugs (Hemiptera: Reduviidae), which transmit Trypanosoma cruzi, the causative agent of Chagas disease, are generally considered nocturnal, but their patterns of activity in controlled settings have rarely be...

    journal_title:Acta tropica

    pub_type: 杂志文章

    doi:10.1016/j.actatropica.2018.06.012

    authors: Wormington JD,Gillum C,Meyers AC,Hamer GL,Hamer SA

    更新日期:2018-09-01 00:00:00

  • Progress on the development of rapid diagnostic tests for foodborne neglected zoonotic helminthiases: A systematic review.

    abstract:BACKGROUND:Foodborne Neglected Zoonotic Helminths (FNZH) are parasites of both economic and public health importance. They include Taenia solium, Echinococcus granulosus sensu lato, Echinococcus multilocularis and Foodborne trematodes (FBT). FNZH are earmarked for major interventions for control, elimination and eradic...

    journal_title:Acta tropica

    pub_type: 杂志文章

    doi:10.1016/j.actatropica.2019.03.030

    authors: Mubanga C,Mwape KE,Phiri IK,Trevisan C,Zulu G,Chabala C,van Damme I,Schmidt V,Dorny P,Gabriël S

    更新日期:2019-06-01 00:00:00

  • Molecular detection of Trypanosoma cruzi in acai pulp and sugarcane juice.

    abstract::Chagas disease, caused by Trypanosoma cruzi affects about 6-8 million people worldwide. Although transmission by triatomine insects has been controlled, other means of transmission maintain the infection. These forms of transmission are responsible for introducing Chagas disease in other non-endemic countries of the w...

    journal_title:Acta tropica

    pub_type: 杂志文章

    doi:10.1016/j.actatropica.2017.08.025

    authors: Mattos EC,Meira-Strejevitch CDS,Marciano MAM,Faccini CC,Lourenço AM,Pereira-Chioccola VL

    更新日期:2017-12-01 00:00:00

  • Geographic variation of Trypanosoma cruzi discrete typing units from Triatoma infestans at different spatial scales.

    abstract::We assessed the diversity and distribution of Trypanosoma cruzi discrete typing units (DTU) in Triatoma infestans populations and its association with local vector-borne transmission levels at various geographic scales. At a local scale, we found high predominance (92.4%) of TcVI over TcV in 68 microscope-positive T. ...

    journal_title:Acta tropica

    pub_type: 杂志文章

    doi:10.1016/j.actatropica.2014.07.014

    authors: Fernández Mdel P,Cecere MC,Lanati LA,Lauricella MA,Schijman AG,Gürtler RE,Cardinal MV

    更新日期:2014-12-01 00:00:00

  • Genotypic and phenotypic characterization of Trypanosoma brucei gambiense isolates from Ibba, South Sudan, an area of high melarsoprol treatment failure rate.

    abstract::Resistance of trypanosomes to melarsoprol is ascribed to reduced uptake of the drug via the P2 nucleoside transporter. The aim of this study was to look for evidence of drug resistance in Trypanosoma brucei gambiense isolates from sleeping sickness patients in Ibba, South Sudan, an area of high melarsoprol failure rat...

    journal_title:Acta tropica

    pub_type: 杂志文章

    doi:10.1016/j.actatropica.2007.07.007

    authors: Maina N,Maina KJ,Mäser P,Brun R

    更新日期:2007-11-01 00:00:00

  • First record of natural vertical transmission of dengue virus in Aedes aegypti from Cuba.

    abstract::While horizontal transmission (human-mosquito-human) of dengue viruses largely determines the epidemiology of the disease, vertical transmission (infected female mosquito- infected offspring) has been suggested as a mechanism that ensures maintenance of the virus during adverse conditions for horizontal transmission t...

    journal_title:Acta tropica

    pub_type: 杂志文章

    doi:10.1016/j.actatropica.2017.07.012

    authors: Gutiérrez-Bugallo G,Rodriguez-Roche R,Díaz G,Vázquez AA,Alvarez M,Rodríguez M,Bisset JA,Guzman MG

    更新日期:2017-10-01 00:00:00

  • Leptospira borgpetersenii from free-living white-eared opossum (Didelphis albiventris): first isolation in Brazil.

    abstract::Leptospirosis is a zoonotic disease that occurs all over the world, caused by bacteria of the genus Leptospira. Marsupial and didelphidae families are considered susceptible to infection caused by a wide range of Leptospira serovars for which they serve as reservoirs. Thirty-three free-living white-eared opossums (Did...

    journal_title:Acta tropica

    pub_type: 杂志文章

    doi:10.1016/j.actatropica.2012.07.009

    authors: Jorge S,Hartleben CP,Seixas FK,Coimbra MA,Stark CB,Larrondo AG,Amaral MG,Albano AP,Minello LF,Dellagostin OA,Brod CS

    更新日期:2012-11-01 00:00:00

  • Species co-occurrence and feeding behavior in sand fly transmission of American cutaneous leishmaniasis in western Venezuela.

    abstract::The structure of the Phlebotomine sand fly community from the Venezuelan Andes was studied using null-model tests. The analyses, at the living zones and altitudes scales, revealed C-scores larger than those expected by random, independently of the collection technique (P < 0.05). These results imply that sand fly spec...

    journal_title:Acta tropica

    pub_type: 杂志文章

    doi:10.1016/j.actatropica.2004.08.001

    authors: Chaves LF,Añez N

    更新日期:2004-11-01 00:00:00

  • In vitro anti-echinococcal activity of octreotide: Additive effect of metformin linked to autophagy.

    abstract::Cystic echinococcosis (CE) is a worldwide zoonosis caused by the Echinococcus granulosus larval stage. The currently available therapy for this disease is based on benzimidazoles, which are rarely curative and cause several adverse effects. Therefore, new treatment options are needed. Octreotide (Oct) is a somatostati...

    journal_title:Acta tropica

    pub_type: 杂志文章

    doi:10.1016/j.actatropica.2019.105312

    authors: Loos JA,Negro P,Cumino AC

    更新日期:2020-03-01 00:00:00

  • Anthelmintic activity of benzimidazole derivatives against Toxocara canis second-stage larvae and Hymenolepis nana adults.

    abstract::The anthelmintic activity of 11 benzimidazole derivatives (A1-A11) and 2 thioureides N,N'-disubstituted (B1-B2) was determined. Each compound and albendazole was tested in vitro against Toxocara canis larvae and in vivo against Hymenolepis nana adult. Compounds A1-A6 and B1-B2 were designed as albendazole prodrugs. Co...

    journal_title:Acta tropica

    pub_type: 杂志文章

    doi:10.1016/j.actatropica.2008.11.014

    authors: Márquez-Navarro A,Nogueda-Torres B,Hernández-Campos A,Soria-Arteche O,Castillo R,Rodríguez-Morales S,Yépez-Mulia L,Hernández-Luis F

    更新日期:2009-03-01 00:00:00

  • First report of equine Pegivirus in South America, Brazil.

    abstract::The human Pegivirus (HPgV, also known as GBV-C virus or hepatitis G virus) is a lymphotropic RNA-virus phylogenetically related to the Hepatitis C virus, which infects approximately 5% of the world's human population. Recently, two novel, presumably hepatotropic, pegiviruses, designated as equine Pegivirus (EPgV) and ...

    journal_title:Acta tropica

    pub_type: 杂志文章

    doi:10.1016/j.actatropica.2015.08.014

    authors: de Souza AJS,Malheiros AP,de Sousa ERP,Moreira ACN,Silva AL,das Chagas AAC,Freitas PEB,Gemaque BS,de Figueiredo HF,de Sá LRM,Dos Santos PDE,Soares MDCP

    更新日期:2015-12-01 00:00:00

  • Activity of azithromycin or erythromycin in combination with antimalarial drugs against multidrug-resistant Plasmodium falciparum in vitro.

    abstract::Azithromycin, an azalide analog of erythromycin was assayed for its in vitro activity against multidrug-resistant Plasmodium falciparum K1 strain by measuring the 3H-hypoxanthine incorporation. Azithromycin caused inhibitory effects on the parasite growth with IC50 and IC90 values of 8.4+/-1.2 microM and 26.0+/-0.9 mi...

    journal_title:Acta tropica

    pub_type: 杂志文章

    doi:10.1016/j.actatropica.2006.10.008

    authors: Nakornchai S,Konthiang P

    更新日期:2006-12-01 00:00:00

  • Fluke egg characteristics for the diagnosis of human and animal fascioliasis by Fasciola hepatica and F. gigantica.

    abstract::In trematodiases, shape and size of the fluke eggs shed with faeces are crucial diagnostic features because of their typically reduced intraspecific variability. In fascioliasis, the usual diagnosis during the biliary stage of infection is based on the classification of eggs found in stools, duodenal contents or bile....

    journal_title:Acta tropica

    pub_type: 杂志文章

    doi:10.1016/j.actatropica.2009.04.005

    authors: Valero MA,Perez-Crespo I,Periago MV,Khoubbane M,Mas-Coma S

    更新日期:2009-08-01 00:00:00

  • Influence of Brugia malayi life stages and BmAFII fraction on experimental Leishmania donovani infection in hamsters.

    abstract::The influence of live Brugia malayi parasites and a Sephadex G-200 fraction of the adult parasite extract (BmAFII) on the progression of Leishmania donovani infection was studied. Inbred hamsters were first infected with B. malayi infective 3rd stage larvae (L3), adult worms or microfilariae (mf), and then with L. don...

    journal_title:Acta tropica

    pub_type: 杂志文章

    doi:10.1016/j.actatropica.2008.01.007

    authors: Murthy PK,Dixit S,Gaur RL,Kumar R,Sahoo MK,Shakya N,Joseph SK,Palne S,Gupta S

    更新日期:2008-05-01 00:00:00

  • Imaging techniques in the evaluation of morbidity in schistosomiasis mansoni.

    abstract::Over the last 20 years a great advance has been observed in many aspects of medicine, and the advent of novel imaging techniques is certainly amongst the most important. In schistosomiasis these new methods caused a revolution in the definition of the clinical forms of the disease and in the evaluation of its complica...

    journal_title:Acta tropica

    pub_type: 杂志文章,评审

    doi:10.1016/j.actatropica.2008.07.009

    authors: Lambertucci JR,dos Santos Silva LC,Andrade LM,de Queiroz LC,Carvalho VT,Voieta I,Antunes CM

    更新日期:2008-11-01 00:00:00

  • Modeling and comparative study of the spread of zoonotic visceral leishmaniasis from Northern to Central Tunisia.

    abstract::During the last twenty years, zoonotic visceral leishmaniasis (ZVL) spread from the north characterized by humid, sub-humid, and semi-arid bio-climates to the arid areas located in Central Tunisia. In order to understand the eco-epidemiological factors involved in the distribution of ZVL, both a sero-epidemiological a...

    journal_title:Acta tropica

    pub_type: 杂志文章

    doi:10.1016/j.actatropica.2017.10.013

    authors: Kaabi B,Zhioua E

    更新日期:2018-02-01 00:00:00

  • Immunogenetic influences on tick resistance in African cattle with particular reference to trypanotolerant N'Dama (Bos taurus) and trypanosusceptible Gobra zebu (Bos indicus) cattle.

    abstract::In sub-Saharan Africa, tick infestation and tick-borne infections together with tsetse-transmitted trypanosomosis arguably constitute the main parasitological disease complex constraining livestock production. Resistance to tick attack and tick-borne micro-organisms (TBMs) varies among different breeds of cattle. The ...

    journal_title:Acta tropica

    pub_type: 杂志文章,评审

    doi:10.1016/s0001-706x(00)00063-2

    authors: Mattioli RC,Pandey VS,Murray M,Fitzpatrick JL

    更新日期:2000-05-31 00:00:00

  • Efficacy of ivermectin to control Strongyloides stercoralis infection in sheltered dogs.

    abstract::In dogs, information on treatments against S. stercoralis infection is rare and anecdotal. The aim of the present work was to evaluate the treatment outcome of S. stercoralis natural infection in sheltered dogs. Furthermore, based on the potential risk of infection, people working in the infected shelter were also tes...

    journal_title:Acta tropica

    pub_type: 杂志文章

    doi:10.1016/j.actatropica.2018.11.014

    authors: Paradies P,Buonfrate D,Iatta R,Iarussi F,Giorli G,Palmieri VO,Sasanelli M,Perandin F,Šlapeta J,Capogna A,Otranto D,Bisoffi Z

    更新日期:2019-02-01 00:00:00

  • Trypanosoma cruzi: effect of pH on in vitro formation of metacyclic trypomastigotes.

    abstract::The formation of metacyclic trypomastigotes of Trypanosoma cruzi in cultures initiated by freshly isolated bloodstream trypomastigotes was studied using three strains of parasites (Peru, CL, and Y) and two different media (L-15 and Grace's medium). Serum (10% newborn calf serum) and various sugars were required for in...

    journal_title:Acta tropica

    pub_type: 杂志文章

    doi:

    authors: Ucros H,Granger B,Krassner SM

    更新日期:1983-06-01 00:00:00

  • Natural Plasmodium infections in Brazilian wild monkeys: reservoirs for human infections?

    abstract::Four hundred and forty-eight samples of total blood from wild monkeys living in areas where human autochthonous malaria cases have been reported were screened for the presence of Plasmodium using microscopy and PCR analysis. Samples came from the following distinct ecological areas of Brazil: Atlantic forest (N=140), ...

    journal_title:Acta tropica

    pub_type: 杂志文章

    doi:10.1016/j.actatropica.2008.05.020

    authors: Duarte AM,Malafronte Rdos S,Cerutti C Jr,Curado I,de Paiva BR,Maeda AY,Yamasaki T,Summa ME,Neves Ddo V,de Oliveira SG,Gomes Ade C

    更新日期:2008-08-01 00:00:00

  • Trypanosoma cruzi persistence at oral inflammatory foci in chronic chagasic patients.

    abstract::The persistence of Trypanosoma cruzi in seropositive individuals, previously diagnosed as chronic chagasic patients (CCP), was detected for the first time in biopsies taken from gingival inflammatory foci processed by polymerase chain reaction (PCR). Seven out of 31 (22.5%) gum samples from selected unquestionably CCP...

    journal_title:Acta tropica

    pub_type: 杂志文章

    doi:10.1016/j.actatropica.2010.12.010

    authors: Añez N,Crisante G,Caraballo F,Delgado W,Parada H

    更新日期:2011-03-01 00:00:00

  • Polymerase chain reaction (PCR) is highly sensitive for diagnosis of mucosal leishmaniasis.

    abstract::We evaluated the use of polymerase chain reaction (PCR) for diagnosis of mucosal leishmaniasis (ML) in an endemic area in Acre, Brazil, where Leishmania braziliensis is present. Leishmania DNA was detected 34 of 35 cases, yielding a positivity rate of 97.1%, which was higher than the positivity rates for all of the ot...

    journal_title:Acta tropica

    pub_type: 杂志文章

    doi:10.1016/j.actatropica.2004.12.003

    authors: Oliveira JG,Novais FO,de Oliveira CI,da Cruz Junior AC,Campos LF,da Rocha AV,Boaventura V,Noronha A,Costa JM,Barral A

    更新日期:2005-04-01 00:00:00

  • Cytochrome P450 2C8*2 allele in Botswana: Human genetic diversity and public health implications.

    abstract::Human cytochrome P450 2C8 is a highly polymorphic gene and shows variation according to ethnicity. The CYP2C8*2 is a slow drug metabolism allele and shows 10-24% frequency in Black populations. The objective of this study was to assess the prevalence of CYP2C8*2 allele in Botswana among the San (or Bushmen) and the Ba...

    journal_title:Acta tropica

    pub_type: 杂志文章

    doi:10.1016/j.actatropica.2016.01.028

    authors: Motshoge T,Tawe L,Muthoga CW,Allotey J,Romano R,Quaye I,Paganotti GM

    更新日期:2016-05-01 00:00:00

  • Molecular detection of Trypanosoma (Kinetoplastida: Trypanosomatidae) in black flies (Diptera: Simuliidae) from Thailand.

    abstract::Trypanosomes are parasitic flagellate protozoans that are important disease causing agents in humans and animals including economically significant livestock. Many blood-sucking insects are known to be vectors of trypanosomes but there is no data for the haematophagous black fly species in Asia. In this study, a molec...

    journal_title:Acta tropica

    pub_type: 杂志文章

    doi:10.1016/j.actatropica.2019.105196

    authors: Thaijarern J,Tangkawanit U,Wongpakam K,Pramual P

    更新日期:2019-12-01 00:00:00