当前位置： SCI文献检索 > ARCHIVES OF PATHOLOGY & LABORATORY MEDICINE期刊下所有文献 > Immunohistochemical analysis of proteins of the Bcl-2 family in pulmonary lymphangioleiomyomatosis: association of Bcl-2 expression with hormone receptor status.

Immunohistochemical analysis of proteins of the Bcl-2 family in pulmonary lymphangioleiomyomatosis: association of Bcl-2 expression with hormone receptor status.

Abstract：

BACKGROUND:Pulmonary lymphangioleiomyomatosis (LAM), a disease of young women, is characterized by proliferation of abnormal smooth muscle cells (LAM cells), which often differ from normal pulmonary smooth muscle cells by frequently having estrogen and progesterone receptors. OBJECTIVE:To evaluate the relationships among several factors related to proliferation and apoptosis in LAM cells, we employed immunohistochemical methods for the localization of Bcl-2 and Mcl-1 (inhibitors of apoptosis), Bax (a promoter of apoptosis), c-Myc (an apoptosis-related oncoprotein), proliferating cell nuclear antigen (an indicator of mitotic activity), and nick end labeling (to identify apoptotic cells) in lung tissues of 9 patients with LAM. RESULTS:In all patients, most LAM cells reacted positively for Bax. The LAM cells were positive for both Bcl-2 and estrogen receptor in 5 patients, positive for only Bcl-2 in 1 patient, positive for only estrogen receptor in another patient, and negative for both in 2 patients. More than 50% of the Bcl-2-positive LAM cells were also positive for estrogen receptor. The reaction for c-Myc was positive in all patients. The immunoreactivity for Bcl-2 and Mcl-1, which inhibit apoptosis, was more intense in LAM cells than in normal vascular and bronchial smooth muscle cells. In 6 patients, more than 50% of the LAM cells were positive for proliferating cell nuclear antigen. Apoptosis was infrequent in LAM cells. CONCLUSIONS:Our results suggest that the expression of Bcl-2 in LAM cells may be related to hormonal regulation, and that by decreasing apoptosis, Bcl-2 and related proteins contribute to the imbalance between proliferation and death of LAM cells.

journal_name

Arch Pathol Lab Med

journal_title

Archives of pathology & laboratory medicine

authors

Usuki J,Horiba K,Chu SC,Moss J,Ferrans VJ

subject

Has Abstract

pub_date

1998-10-01 00:00:00

pages

895-902

issue

eissn

0003-9985

issn

1543-2165

journal_volume

122

pub_type

临床试验,杂志文章

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Immunohistochemical analysis of proteins of the Bcl-2 family in pulmonary lymphangioleiomyomatosis: association of Bcl-2 expression with hormone receptor status.