Purification and molecular cloning of a novel calcium-binding protein, p26olf, in the frog olfactory epithelium.

Abstract:

:Olfactory adaptation requires the change of intracellular calcium concentration during stimuli. To contribute in the study of the molecular mechanism of calcium-dependent regulations in olfactory receptor cells, we isolated a novel 26-kDa Ca2+-binding protein named p26olf from the frog olfactory epithelium after four chromatographical steps. Based on the partial amino acid sequences of the proteolysed fragments of p26olf, we obtained a cDNA clone that encodes p26olf. The analysis of its amino acid sequence revealed that p26olf consists of two S-100-like regions aligned sequentially with a calculated molecular mass of 24,493. Northern blot analysis showed that p26olf is expressed in the frog olfactory epithelium and also in other tissues. Immunoreactivity against p26olf was detected in the cilia layer of the olfactory epithelium. These results suggest that p26olf is a dimeric form of S-100 proteins and is involved in the olfactory transduction or adaptation.

authors

Miwa N,Kobayashi M,Takamatsu K,Kawamura S

doi

10.1006/bbrc.1998.9571

subject

Has Abstract

pub_date

1998-10-29 00:00:00

pages

860-7

issue

3

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(98)99571-7

journal_volume

251

pub_type

杂志文章
  • CREB DNA binding activation by a 50-Hz magnetic field in HL60 cells is dependent on extra- and intracellular Ca(2+) but not PKA, PKC, ERK, or p38 MAPK.

    abstract::To investigate the possible mechanism of gene transcription changes induced by magnetic field (MF), we examined the DNA binding behavior of the transcription factor cyclic-AMP responsive element binding protein (CREB) in HL60 cells after exposure to a 0.1mT 50-Hz extremely low frequency (ELF) sinusoidal MF by a gel sh...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/s0006-291x(02)02022-3

    authors: Zhou J,Yao G,Zhang J,Chang Z

    更新日期:2002-08-30 00:00:00

  • Binding sites for inositol trisphosphate in the bovine adrenal cortex.

    abstract::Binding sites for inositol trisphosphate (IP3) have been identified in bovine adrenal cortex, employing [32P]IP3 prepared from human erythrocytes radiolabeled with [32P]ATP. IP3 was bound to adrenal microsomes with high affinity (Kd = 5 nM) and low capacity (186 fmol/mg protein). During kinetic studies, half-maximal b...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/0006-291x(85)90939-8

    authors: Baukal AJ,Guillemette G,Rubin R,Spät A,Catt KJ

    更新日期:1985-12-17 00:00:00

  • The binding cavity of mouse major urinary protein is optimised for a variety of ligand binding modes.

    abstract::(15)N and (1)HN chemical shift data and (15)N relaxation studies have been used to characterise the binding of N-phenyl-naphthylamine (NPN) to mouse major urinary protein (MUP). NPN binds in the beta-barrel cavity of MUP, hydrogen bonding to Tyr120 and making extensive non-bonded contacts with hydrophobic side chains....

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2009.10.133

    authors: Pertinhez TA,Ferrari E,Casali E,Patel JA,Spisni A,Smith LJ

    更新日期:2009-12-25 00:00:00

  • A secondary structure prediction of the hemorrhagic metalloprotease family.

    abstract::A secondary structure has been predicted for the hemorrhagic metalloproteases using a method developed in Zurich that extracts structural information from patterns of conservation and variation in homologous protein sequences. This prediction tests the limits of the method when applied to a small number of homologous ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1006/bbrc.1993.1856

    authors: Gerloff DL,Jenny TF,Knecht LJ,Benner SA

    更新日期:1993-07-15 00:00:00

  • Divergent effects of filamin and tropomyosin on actin filaments bundling.

    abstract::Filamin increases and tropomyosin decreases the susceptibility of F-actin to form bundles of filaments in the presence of polyethylene glycol 6000. The two proteins, which are located in the leading edge and in the internal part of the cell, respectively, are thus likely to display divergent effects on the microfilame...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/0006-291x(90)90637-3

    authors: Grazi E,Trombetta G,Guidoboni M

    更新日期:1990-03-30 00:00:00

  • Fission yeast cdc25 is a cell-cycle regulated protein.

    abstract::Fission yeast cell division is initiated by the cdc2/cdc13-cyclin protein kinase which in its catalytically active state comprises the mitotic inducer. During interphase the cdc2/cyclin complex is assembled in an inactive state that requires cdc25+ gene function for M-phase activation. The cdc25+ product, a 76 kd phos...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/0006-291x(90)91765-k

    authors: Ducommun B,Draetta G,Young P,Beach D

    更新日期:1990-02-28 00:00:00

  • Synthesis of a nitric oxide-like factor from L-arginine by rat serosal mast cells: stimulation of guanylate cyclase and inhibition of platelet aggregation.

    abstract::Rat serosal mast cells were tested for their ability to generate a nitric oxide-like factor by two bioassay systems: inhibition of platelet aggregation and stimulation of mast cell guanylate cyclase. Incubation of rat serosal mast cells with human washed platelets resulted in an inhibition of thrombin-induced platelet...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/0006-291x(90)90372-t

    authors: Salvemini D,Masini E,Anggard E,Mannaioni PF,Vane J

    更新日期:1990-06-15 00:00:00

  • Characterization and expression of a novel human fatty acid-binding protein: the epidermal type (E-FABP).

    abstract::Using PAGE--Autoradioblotting technique we have characterized an E--FABP in human epidermal cells that is distinct from liver-, heart-, intestine- and adipose tissue-FABPs. FABP radiobinding analysis was performed directly on protein extracts without prior partial purification. E-FABP has a Mr of approximately 15 kDa ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1006/bbrc.1993.1073

    authors: Siegenthaler G,Hotz R,Chatellard-Gruaz D,Jaconi S,Saurat JH

    更新日期:1993-01-29 00:00:00

  • MicroRNA isolation and stability in stored RNA samples.

    abstract::MicroRNAs (miRNAs) are small RNA molecules, which act as post-transcriptional regulators of a gene expression, with important functions within the cell physiology. Whilst many authors have focused on the study of miRNA expression in physiological and pathological processes, various technical variables related to miRNA...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章,评审

    doi:10.1016/j.bbrc.2009.09.061

    authors: Mraz M,Malinova K,Mayer J,Pospisilova S

    更新日期:2009-12-04 00:00:00

  • Fludrocortisone stimulates erythropoietin production in the intercalated cells of the collecting ducts.

    abstract::Erythropoietin has been thought to be secreted to plasma soon after the production because of the difficulty of Western blot analysis and immunohistochemistry. We established the new methods of Western blot analysis and immunohistochemistry. Using the new methods, we investigated the effects of aldosterone and fludroc...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2018.08.102

    authors: Yasuoka Y,Izumi Y,Nagai T,Fukuyama T,Nakayama Y,Inoue H,Horikawa K,Kimura M,Nanami M,Yanagita K,Oshima T,Yamazaki T,Uematsu T,Yamamura R,Kobayashi N,Shimada Y,Nagaba Y,Nakanishi T,Yamashita T,Mukoyama M,Sato Y,K

    更新日期:2018-09-18 00:00:00

  • The Foxc2 transcription factor regulates tumor angiogenesis.

    abstract::The Forkhead/Fox transcription factor Foxc2 is a critical regulator of vascular development. However, the role of Foxc2 in pathological angiogenesis in cancer remains unknown. Here we show that FoxC2 is highly expressed in human breast and colonic tumors and in the tumor endothelium in human and mouse melanomas. Using...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2010.01.015

    authors: Sano H,Leboeuf JP,Novitskiy SV,Seo S,Zaja-Milatovic S,Dikov MM,Kume T

    更新日期:2010-02-05 00:00:00

  • Characterization of rodent epidermal growth factor receptor transcripts using a mouse genomic probe.

    abstract::Two genomic DNA clones encoding part of the mouse epidermal growth factor (EGF) receptor were isolated: an 8.0 kb insert that hybridized with the human EGF receptor kinase domain; and a 2.4 kb insert that mapped between the kinase and 3' autophosphorylation domains of the human EGF receptor gene. The 2.4 kb insert was...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/s0006-291x(86)80158-9

    authors: Hung MC,Thompson KL,Chiu IM,Rosner MR

    更新日期:1986-12-30 00:00:00

  • A knock-in mouse model of N-terminal R420W mutation of cardiac ryanodine receptor exhibits arrhythmogenesis with abnormal calcium dynamics in cardiomyocytes.

    abstract::Cardiac ryanodine receptor gene (RyR2) mutations cause fatal arrhythmogenic diseases such as catecholaminergic polymorphic ventricular tachycardia and arrhythmogenic right ventricular cardiomyopathy. The N-terminal region of RyR2 is one of the hot spots for mutations. In this study, we investigated cardiac phenotypes ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2014.08.132

    authors: Okudaira N,Kuwahara M,Hirata Y,Oku Y,Nishio H

    更新日期:2014-09-26 00:00:00

  • Global cerebral ischemia induces increased expression of multiple retrotransposons.

    abstract::Retrotransposons (RTs) account for ~45% of the mammalian genome. They are capable of inserting into new genomic locations, which can result in deleterious outcomes. We examined the response of nine RTs to global cerebral ischemia (GCI) and explored the DNA methylation status of the two significantly altered RTs. Seven...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2013.03.116

    authors: Wang S,Kelly S

    更新日期:2013-05-10 00:00:00

  • Fluorescence polarization study of lipids and membranes prepared from brain hemispheres of a hibernating mammal.

    abstract::The physical behavior of total lipids, microsomes and microsomal lipids prepared from brain hemispheres of European Hamsters (Cricetus cricetus) was approached by the measure of the fluorescence polarization of the probe 1,6-diphenyl 1,3,5-hexatriene. We compare in this study the results obtained for two critical peri...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/0006-291x(84)91665-6

    authors: Montaudon D,Robert J,Canguilhem B

    更新日期:1984-02-29 00:00:00

  • The relation between two molecular species of P-450 in adult testis and 17 alpha-hydroxylase and 17,20-lyase activities.

    abstract::Two P-450s from adult pig testis were purified to specific contents of 11.2 and 12.0 nmol P-450/mg protein and shown to have minimum molecular weights of 45,000 and 46,000, respectively. The absorption spectra were typical of P-450s. The P-450s were separated from the two fractions by CM-C50 Sephadex column chromatogr...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1006/bbrc.1996.0094

    authors: Kuwada M,Takizawa N,Sone Y

    更新日期:1996-01-17 00:00:00

  • Genetic deletion of miR-204 improves glycemic control despite obesity in db/db mice.

    abstract::MicroRNAs (miRs) are small non-coding RNAs that regulate the target gene expression. A change in miR profile in the pancreatic islets during diabetes is known, and multiple studies have demonstrated that miRs influence the pancreatic β-cell function. The miR-204 is highly expressed in the β-cells and reported to regul...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2020.08.077

    authors: Gaddam RR,Kim YR,Li Q,Jacobs JS,Gabani M,Mishra A,Promes JA,Imai Y,Irani K,Vikram A

    更新日期:2020-11-05 00:00:00

  • Effect of arginine and glucagon on perifused purified beta cells.

    abstract::The secretory response of purified beta cells was studied employing a newly developed microperifusion technique. In control experiments, initial high insulin release up to 50 minutes followed by a steady state basal level was observed up to five hours. The changes in hydrodynamic pressure of perifusion produced small,...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/s0006-291x(88)80373-5

    authors: Bandisode MS

    更新日期:1988-03-15 00:00:00

  • Biophysical characterization of the breast cancer-related BIG3-PHB2 interaction: Effect of non-conserved loop region of BIG3 on the structure and the interaction.

    abstract::Brefeldin A-inhibited guanine nucleotide-exchange protein 3 (BIG3) interacts with and inhibits the tumor suppressor function of prohibitin-2 (PHB2), and recent in vivo studies have demonstrated that the BIG3-PHB2 interaction is a promising target for breast cancer therapy. However, little biophysical characterization ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2019.08.028

    authors: Chigira T,Nagatoishi S,Takeda H,Yoshimaru T,Katagiri T,Tsumoto K

    更新日期:2019-10-08 00:00:00

  • Effect of cysteamine on redox-sensitive thiol-containing proteins in the duodenal mucosa.

    abstract::Recent studies from our laboratory demonstrated that Egr-1 is upregulated in the rat duodenal mucosa during cysteamine-induced duodenal ulceration and that antisense egr-1 oligonucleotide aggravates the duodenal ulcers. This study was aimed to determine the effects of cysteamine on redox-sensitive Egr-1 transcriptiona...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2003.08.092

    authors: Khomenko T,Deng X,Jadus MR,Szabo S

    更新日期:2003-10-03 00:00:00

  • SNHG11 promotes cell proliferation in colorectal cancer by forming a positive regulatory loop with c-Myc.

    abstract::Dysregulation of long non-coding RNAs (lncRNA) have long been linked to the onset and development of colorectal cancer (CRC), yet the underlying mechanisms remain elusive. Small nucleolar RNA host gene 11 (SNHG11) is a novel lncRNA with few information about its role in development and progression of CRC. Here, we fou...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2020.05.002

    authors: Huang W,Dong S,Cha Y,Yuan X

    更新日期:2020-07-05 00:00:00

  • Reaction of 5'-p-fluorosulfonylbenzoyladenosine with the catalytic and AMP allosteric sites of microsomal HMG-CoA reductase kinase.

    abstract::The nucleotide analogue 5-p-fluorosulfonylbenzoyladenosine reacts with rat liver microsomal 3-hydroxy-3-methylglutaryl-CoA reductase kinase, causing a rapid loss of the AMP activation capacity and a slower inactivation of the catalytic activity. The rate constant for loss of AMP activation is eleven times higher (K1 =...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/s0006-291x(87)80232-2

    authors: Ferrer A,Caelles C,Hegardt FG

    更新日期:1987-11-13 00:00:00

  • Decrease of NADH in HeLa cells in the presence of transferrin or ferricyanide.

    abstract::The short-term incubation of HeLa cells in the presence of diferric transferrin or ferricyanide, which are reduced externally by the transplasma membrane reductase, produces a stoichiometric decrease in NADH and increase in NAD+, which is stimulated by insulin. The NADP/NADPH ratio does not change during 15 min incuba...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/0006-291x(86)90949-6

    authors: Navas P,Sun IL,Morré DJ,Crane FL

    更新日期:1986-02-26 00:00:00

  • A severe de novo methylation of episomal vectors by human ES cells.

    abstract::Episomal vectors can allow efficient genetic modification of cells and have the potential advantage of avoiding chromosomal position of integration effects. Here we explore the use of an Epstein-Barr virus-based episomal vector with human embryonic stem (ES) cells, and find high initial transfection rates, but a rapid...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2006.08.175

    authors: Kameda T,Smuga-Otto K,Thomson JA

    更新日期:2006-11-03 00:00:00

  • Conserved signal peptide of Notch3 inhibits interaction with proteasome.

    abstract::The Notch3 N-terminal sequence is conserved across several mammalian species but diverges from the three other Notch proteins. We determined the significance of the N-terminal sequence using deletion mutants. The first 39 amino acids are required for Notch3 receptor expression, processing, and functional activity. In ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2007.01.151

    authors: Zhang Y,Jia L,Lee SJ,Wang MM

    更新日期:2007-03-30 00:00:00

  • Pre-pore oligomer formation by Vibrio cholerae cytolysin: insights from a truncated variant lacking the pore-forming pre-stem loop.

    abstract::Vibrio cholerae cytolysin (VCC), a β-barrel pore-forming toxin (β-PFT), induces killing of the target eukaryotic cells by forming heptameric transmembrane β-barrel pores. Consistent with the β-PFT mode of action, binding of the VCC toxin monomers with the target cell membrane triggers formation of pre-pore oligomeric ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2013.11.078

    authors: Paul K,Chattopadhyay K

    更新日期:2014-01-03 00:00:00

  • Insulin-dependent phosphatidylinositol 3'-kinase activity co-precipitates with insulin receptor in human circulating mononuclear cells.

    abstract::In order to establish a working cellular model for the study of post-receptor signaling events, insulin-dependent phosphatidylinositol 3' (PtdIns 3')-kinase activity was investigated in circulating mononuclear cells from normal subjects. The p85 alpha regulatory subunit of PtdIns 3'-kinase co-precipitated with activat...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1006/bbrc.1995.1494

    authors: Renard E,Grigorescu F,Lavabre C,Kahn CR

    更新日期:1995-04-06 00:00:00

  • LAMP-2 mediates oxidative stress-dependent cell death in Zn2+-treated lung epithelium cells.

    abstract::Zinc is an essential element for the biological system. However, excessive exogenous Zn2+ would disrupt cellular Zn2+ homeostasis and cause toxicity. In particular, Zinc salts or ZnO nanoparticles exposure could induce respiratory injury. Although previous studies have indicated that organelle damage (including mitoch...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2017.05.030

    authors: Qin X,Zhang J,Wang B,Xu G,Zou Z

    更新日期:2017-06-17 00:00:00

  • Transcriptional activity of DNMT3B in pancreatic cancer cells: effects of -149 (C→T) promoter polymorphism.

    abstract::Polymorphic C-to-T change in the promoter region of DNA-methyltransferase-3B (DNMT3B) gene is associated with risk of several cancers. The aim of this study was to investigate the effect of DNMT3B promoter genetic variant on its transcriptional activity and to compare activity in several pancreatic cell lines. DNMT3B ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2011.07.115

    authors: Xiao Y,Word B,Hammons G,Lyn-Cook B

    更新日期:2011-11-18 00:00:00

  • Reversal of new-onset type 1 diabetes in mice by syngeneic bone marrow transplantation.

    abstract::Autologous hematopoietic stem cell transplantation (HSCT) has recently been performed as a novel strategy to treat patients with new-onset type 1 diabetes (T1D). However, the mechanism of autologous HSCT-induced remission of diabetes remains unknown. In order to help clarify the mechanism of remission-induction follow...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2008.07.016

    authors: Wen Y,Ouyang J,Yang R,Chen J,Liu Y,Zhou X,Burt RK

    更新日期:2008-09-19 00:00:00