Abstract:
:The proteasome activator protein PA28 or 11 S regulator may play an important role in facilitating the generation of peptides for presentation by the MHC class I system. PA28 is composed of two homologous subunits termed alpha and beta. Removal of the carboxyl terminal tyrosine of the alpha subunit of PA28 abolishes activity (X. Song et al., 1997, J. Biol. Chem. 272, 27994-28000). To explore the structural basis of this effect the des-tyrosyl analog of PA28alpha prepared by site-directed mutagenesis and PA28alpha were expressed at high levels in a baculovirus system and purified by FPLC. Des-tyrosyl-PA28alpha neither stimulated the proteasome nor competed with PA28alpha for binding to the proteasome. Hydrophobic interaction chromatography revealed that the hydrophobicity of the mutant protein was considerably greater than PA28alpha. When the mutant protein was chromatographed on a calibrated Superose 6 column a mixture of approximately 25% oligomer and 75% monomer was found. The oligomer weakly stimulated the proteasome but this molecule was labile. Very low concentrations of SDS (0.005%) dissociated PA28alpha and abolished its stimulatory activity. It is concluded that the lack of activity of des-tyrosyl-PA28alpha is due to conformational changes resulting in dissociation and that the oligomeric form of PA28alpha is required for activation.
journal_name
Arch Biochem Biophysjournal_title
Archives of biochemistry and biophysicsauthors
Wilk S,Chen WE,Magnusson RPdoi
10.1006/abbi.1998.0918subject
Has Abstractpub_date
1998-11-15 00:00:00pages
283-90issue
2eissn
0003-9861issn
1096-0384pii
S0003-9861(98)90918-3journal_volume
359pub_type
杂志文章abstract::B4-2-1 cells (Lec15 cells) are Chinese hamster ovary cells deficient in mannosylphosphoryldolichol synthase activity. They synthesize the truncated lipid intermediate Man5GlcNAc2-P-P-dolichol rather than the Glc3Man9GlcNAc2-P-P-dolichol synthesized by wild-type cells. In this report we present evidence that these cell...
journal_title:Archives of biochemistry and biophysics
pub_type: 杂志文章
doi:10.1016/0003-9861(92)90268-2
更新日期:1992-12-01 00:00:00
abstract::The binding of Toxin A isolated from Clostridium difficile to rabbit erythrocyte glycolipids has been studied. Total lipid extracts from rabbit erythrocytes were subjected to thin-layer chromatography and toxin-binding glycolipids detected by using 125I-labeled Toxin A in a direct binding overlay technique. Two major ...
journal_title:Archives of biochemistry and biophysics
pub_type: 杂志文章
doi:10.1016/0003-9861(87)90561-3
更新日期:1987-08-15 00:00:00
abstract::The vesicle-to-micelle structural transitions that occurred in the interaction of sodium dodecyl sulfate with phosphatidylcholine vesicles were studied at the equilibrium by means of dynamic light scattering (at different scattering angles) and freeze-fracture electron microscopy techniques. The incorporation of surfa...
journal_title:Archives of biochemistry and biophysics
pub_type: 杂志文章
doi:10.1006/abbi.1999.1267
更新日期:1999-07-15 00:00:00
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更新日期:2019-05-15 00:00:00
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更新日期:1994-11-01 00:00:00
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pub_type: 杂志文章
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更新日期:2008-02-01 00:00:00
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journal_title:Archives of biochemistry and biophysics
pub_type: 杂志文章
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更新日期:2005-05-15 00:00:00
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journal_title:Archives of biochemistry and biophysics
pub_type: 杂志文章
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更新日期:1992-08-01 00:00:00
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journal_title:Archives of biochemistry and biophysics
pub_type: 杂志文章
doi:10.1006/abbi.1993.1262
更新日期:1993-05-15 00:00:00
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journal_title:Archives of biochemistry and biophysics
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journal_title:Archives of biochemistry and biophysics
pub_type: 杂志文章
doi:10.1006/abbi.1993.1323
更新日期:1993-07-01 00:00:00
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journal_title:Archives of biochemistry and biophysics
pub_type: 杂志文章
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journal_title:Archives of biochemistry and biophysics
pub_type: 杂志文章
doi:10.1016/j.abb.2013.03.003
更新日期:2013-07-15 00:00:00
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pub_type: 杂志文章
doi:10.1016/0003-9861(89)90237-3
更新日期:1989-08-01 00:00:00
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journal_title:Archives of biochemistry and biophysics
pub_type: 杂志文章
doi:10.1016/j.abb.2019.05.003
更新日期:2019-06-15 00:00:00
abstract::Glucose oxidase reduces nitroblue tetrazolium, or ferricytochrome c, faster anaerobically than aerobically. This result is inconsistent with the conclusion that GO2 can reduce O2 to O2- which is then responsible for the reduction of NBT and of cytochrome c. Nevertheless, the aerobic reductions are partially inhibitabl...
journal_title:Archives of biochemistry and biophysics
pub_type: 杂志文章
doi:10.1006/abbi.1995.1247
更新日期:1995-04-20 00:00:00
abstract::Studies in vascular smooth muscle cells suggest that, angiotensin II (Ang II)-mediated cellular response requires transactivation of epidermal growth factor receptor (EGF-R), and involves tyrosine phosphorylation of caveolin-1. Here we demonstrate that, exposure of WB rat liver cells to Ang II does not cause transacti...
journal_title:Archives of biochemistry and biophysics
pub_type: 杂志文章
doi:10.1016/j.abb.2006.10.011
更新日期:2007-01-01 00:00:00
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pub_type: 杂志文章,评审
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journal_title:Archives of biochemistry and biophysics
pub_type: 杂志文章
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journal_title:Archives of biochemistry and biophysics
pub_type: 杂志文章
doi:10.1006/abbi.2000.1801
更新日期:2000-06-01 00:00:00
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journal_title:Archives of biochemistry and biophysics
pub_type: 杂志文章
doi:10.1016/j.abb.2011.07.013
更新日期:2011-10-01 00:00:00
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journal_title:Archives of biochemistry and biophysics
pub_type: 杂志文章
doi:10.1016/0003-9861(85)90816-1
更新日期:1985-05-15 00:00:00
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journal_title:Archives of biochemistry and biophysics
pub_type: 杂志文章
doi:10.1006/abbi.1994.1025
更新日期:1994-01-01 00:00:00
abstract::The quinoid pigments pthiocol, produced by Mycobacterium tuberculosis, and pyocyanine, produced by Pseudomonas aeruginosa, were examined for their effects on O2.- production in cultured human lung epithelial-like A549 cells. Intracellular O2.- levels were measured using the O2.-sensitive aconitase(s), and rates of O2....
journal_title:Archives of biochemistry and biophysics
pub_type: 杂志文章
doi:10.1006/abbi.1996.0390
更新日期:1996-09-01 00:00:00
abstract::Previous work has shown that catalytic function is associated exclusively with the C-terminal half of the Type I isozyme of mammalian hexokinase. In contrast, we now demonstrate that both halves of the Type II isozyme possess comparable catalytic activities. Mutation of a catalytically important Ser residue to Ala at ...
journal_title:Archives of biochemistry and biophysics
pub_type: 杂志文章
doi:10.1006/abbi.1996.0186
更新日期:1996-05-01 00:00:00
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journal_title:Archives of biochemistry and biophysics
pub_type: 杂志文章
doi:10.1016/0003-9861(85)90628-9
更新日期:1985-01-01 00:00:00
abstract::Ca(2+)-loaded rat liver mitochondria treated with 3,5,3'-triiodothyronine (T3) undergo nonspecific inner membrane permeabilization, as evidenced by mitochondrial swelling, a decrease in membrane potential (delta psi), and an increase in the rate of oxygen uptake. T3 analogues thyroxine (T4), 3',5'-diiodothyronine (T2)...
journal_title:Archives of biochemistry and biophysics
pub_type: 杂志文章
doi:10.1006/abbi.1998.0657
更新日期:1998-06-01 00:00:00
abstract::Diquat is a hepatotoxin whose toxicity in vivo and in vitro is mediated by redox cycling and greatly enhanced by pretreatment with 1,3-bis(2-chloroethyl)-1-nitrosourea (BCNU), an inhibitor of glutathione reductase. The mechanism by which redox cycling mediates diquat cytotoxicity is unclear, however. Here, we have att...
journal_title:Archives of biochemistry and biophysics
pub_type: 杂志文章
doi:10.1016/0003-9861(87)90466-8
更新日期:1987-11-15 00:00:00
abstract:BACKGROUND:Proteomics is expected to provide novel insights in the underlying pathophysiology of type 2 diabetes mellitus. In the present study, we aimed to identify and biochemically characterize proteins associated with diabetes mellitus in a Qatari population. METHODS:In a diabetes case-control study (175 cases, 16...
journal_title:Archives of biochemistry and biophysics
pub_type: 杂志文章
doi:10.1016/j.abb.2020.108476
更新日期:2020-08-15 00:00:00