Effects of resuscitation fluids on T cell immune responses.

Abstract:

:This study was designed to determine whether dextran, gelatin or hydroxyethyl starch-based colloidal resuscitation fluids (CRF) are inhibitory to T lymphocyte activation and mitogenesis in vitro. Isolated peripheral blood lymphocytes from normal donors were activated with mitogen (PHA) and cultured in up to 50% v:v CRF. Dual-label flow cytometry for CD69 and CD25 were used to assess early and full T cell activation responses, respectively, and thymidine incorporation was used to assess mitogenesis. T cell activation and mitogenic responses were not inhibited in the presence of CRF, implying that any systemic immunodepression associated with CRF infusion is not directly related to CRF-mediated impairment of T cell activation.

journal_name

Br J Anaesth

authors

Sillett HK,Whicher JT,Trejdosiewicz LK

doi

10.1093/bja/81.2.242

subject

Has Abstract

pub_date

1998-08-01 00:00:00

pages

242-3

issue

2

eissn

0007-0912

issn

1471-6771

pii

S0007-0912(17)39653-8

journal_volume

81

pub_type

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