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Lipofection indirectly increases expression of endogenous major histocompatibility complex class I molecules on tumor cells.

Abstract：

:Direct intratumoral injection of a lipid/DNA complex encoding an allogeneic major histocompatibility complex (MHC) class I molecule leads to regression of both an immunogenic murine tumor and also melanoma lesions in some patients. We have sought to understand the mechanism(s) for this augmentation of antitumor activity. While optimizing parameters for in vitro gene transfer into the D5 subclone of B16BL6, it was noted that lipofected tumors not only expressed the new alloantigen but also exhibited increased expression of endogenous MHC class I, both H-2 Kb and H-2 Db. This increase in expression was not restricted to the small percentage of cells that expressed the transfected gene, but appeared to affect the majority of cells in culture. Class I expression was not increased by lipopolysaccharide, DNA alone, lipid, or lipid/lipopolysaccharide mixtures. Enhanced class I expression required a DNA/lipid complex and was greatest when parameters optimized for gene transfer of the alloantigen were used. All DNA plasmids tested had this effect, including one plasmid whose DNA was not transcribed because it lacked an expression cassette. Because of the critical role that MHC class I antigens play in immune recognition, we propose that lipid complex-mediated gene transfer may provide immunological advantages beyond those that are attributable to expression of the specific gene transferred.

journal_name

Cancer Gene Ther

journal_title

Cancer gene therapy

authors

Fox BA,Drury M,Hu HM,Cao Z,Huntzicker EG,Qie W,Urba WJ

subject

Has Abstract

pub_date

1998-09-01 00:00:00

pages

307-12

issue

5

eissn

0929-1903

issn

1476-5500

journal_volume

5

pub_type

杂志文章

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