Abstract:
:To assess in vivo effects of antioxidants on vascular cell adhesion molecule (VCAM)-1 expression, circulating soluble VCAM-1 and intraerythrocytic reduced glutathione (GSH) and GSH disulphide (GSSG) concentrations were evaluated in non-insulin-dependent diabetic patients without complications (9 men, 6 women, 48 +/- 6 years old) before and after 1 month of either oral N-acetyl-L-cysteine (1.200 mg/day) or placebo treatments, given in randomized, cross-over, double-blind fashion. Ten healthy subjects (7 men, 3 women, 52 +/- 4 years old) served as control subjects. Baseline plasma VCAM-1 concentrations were higher (p = 0.007) in non-insulin-dependent diabetic patients (707.9 +/- 52.5 ng/ml) than in control subjects (627.3 +/- 84.6 ng/ml). Intraerythrocytic GSSG content was higher (non-insulin dependent diabetic patients: 0.618 +/- 0.185 micromol/g Hb; control subjects: 0.352 +/- 0.04 micromol/g Hb, p = 0.0002), whereas intraerythrocytic GSH concentrations were lower (p = 0.001) in non-insulin dependent diabetic patients (6.0 +/- 0.7 micromol/g Hb) than in control subjects (7.1 +/- 0.5 micromol/g Hb). The mean GSH:GSSG ratio was also lower (p = 0.0001) in the first (10.9 +/- 4.5) than in the second group (20.2 +/- 1.4). Circulating VCAM-1 and intraerythrocytic GSH concentrations were negatively correlated in non-insulin diabetic patients (r = 0.605, p = 0.01). Treatment with N-acetyl-L-cysteine decreased plasma VCAM-1 (p = 0.01) and intraerythrocytic GSSG (p = 0.006) but increased GSH concentrations (p = 0.04) and the GSH:GSSG ratio (p = 0.004) in non-insulin dependent diabetic patients. Our data indicate that the vascular endothelium is activated in non-insulin dependent diabetes. Antioxidant treatment counterbalanced such endothelial activation. Thus, antioxidant agents might protect against oxidant-related upregulation of endothelial adhesion molecules and slow down the progression of vascular damage in non-insulin dependent diabetes.
journal_name
Diabetologiajournal_title
Diabetologiaauthors
De Mattia G,Bravi MC,Laurenti O,Cassone-Faldetta M,Proietti A,De Luca O,Armiento A,Ferri Cdoi
10.1007/s001250051082subject
Has Abstractpub_date
1998-11-01 00:00:00pages
1392-6issue
11eissn
0012-186Xissn
1432-0428journal_volume
41pub_type
临床试验,杂志文章,随机对照试验相关文献
DIABETOLOGIA文献大全abstract:AIMS/HYPOTHESIS:Pancreatic islet transplantation (PIT) offers a physiological treatment for type 1 diabetes, but the failure of islet engraftment hinders its application. The female hormone 17β-oestradiol (E2) favours islet survival and stimulates angiogenesis, raising the possibility that E2 may enhance islet engraftm...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s00125-012-2764-1
更新日期:2013-02-01 00:00:00
abstract::Human crude and recombinant interleukin 1 (IL-1) was found to dose- and time-dependently affect the biosynthesis of (pro)insulin in isolated rat islets of Langerhans. Incubation of rat islets with either 0.5 U/ml or 5 U/ml of crude IL-1 for 1 h had no detectable effect on (pro)insulin biosynthesis. After 24 hours of e...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/BF00279615
更新日期:1987-07-01 00:00:00
abstract:AIMS/HYPOTHESIS:Leptin resistance is generally considered a consequence of obesity. We postulated that leptin resistance is associated with diminished hypothalamic leptin signalling capacity and that leptin resistance is causal to obesity. We assessed maximal leptin-mediated binding of the transcription factor signal t...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s00125-005-1763-x
更新日期:2005-06-01 00:00:00
abstract:AIMS/HYPOTHESIS:Previous studies show that inflammatory cytokines play a part in the development of insulin resistance. Thiazolidinediones were developed as insulin-sensitizing drugs and are ligands for the peroxisome proliferator-activated receptory (PPARgamma). We hypothesized that the anti-diabetic mechanism of thia...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s001250051218
更新日期:1999-06-01 00:00:00
abstract::An in vitro method for detecting IgE insulin antibody using the principle of the radioallergosorbent test (RAST) is described. In six patients with insulin allergy, the RAST values were higher than in normal persons or insulin-treated diabetics without insulin-allergy. No differences were observed between normal perso...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/BF00429705
更新日期:1978-01-14 00:00:00
abstract::Hyperglycaemia has been shown to play a central part in diabetic vascular disease, which is also influenced by individual background. Hyperglycaemia initiates the pathogenetic sequence through a series of interrelated biochemical abnormalities, including increased flux through the polyol and hexosamine pathways, oxida...
journal_title:Diabetologia
pub_type:
doi:10.1007/s001250051676
更新日期:2001-06-01 00:00:00
abstract:AIMS/HYPOTHESIS:Insulin control of glucose output is a major mechanism by which appropriate amounts of glucose are produced to supply energy to the central nervous system, without causing long-term increases of the plasma glucose concentration. It is hypothesised that the primary route by which insulin maintains contro...
journal_title:Diabetologia
pub_type: 杂志文章,评审
doi:10.1007/s001250051474
更新日期:2000-07-01 00:00:00
abstract:AIMS/HYPOTHESIS:Diabetes promotes cerebral neovascularisation via increased vascular endothelial growth factor (VEGF) angiogenic signalling. Roundabout-4 (ROBO4) protein is an endogenous inhibitor of VEGF signalling that stabilises the vasculature. Yet, how diabetes affects ROBO4 function remains unknown. We hypothesis...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s00125-017-4214-6
更新日期:2017-04-01 00:00:00
abstract:AIMS/HYPOTHESIS:Brain natriuretic peptide (BNP) is a potent vasorelaxing and natriuretic peptide that is secreted from the heart and has cardioprotective properties. We have previously generated hypotensive transgenic mice (BNP-Tg mice) that overproduce BNP in the liver, which is released into the circulation. Using th...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s00125-006-0352-y
更新日期:2006-10-01 00:00:00
abstract:AIMS/HYPOTHESIS:Abnormal serum IGF-1 levels are associated with an increased risk of type 2 diabetes and cardiovascular disease. However, the causal role of IGF-1 levels within the normal range in cardiometabolic disease remains unclear. We employed Mendelian randomisation to explore the associations between geneticall...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s00125-020-05190-9
更新日期:2020-09-01 00:00:00
abstract:AIMS/HYPOTHESIS:The aim of this study was to examine the prevalence and nature of mutations in HNF4alpha/MODY1, GCK/MODY2 and HNF-1alpha/MODY3 genes in Czech subjects with clinical diagnosis of MODY. METHODS:We studied 61 unrelated index probands of Czech origin (28 males, 33 females) with a clinical diagnosis of MODY...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s00125-002-1010-7
更新日期:2003-02-01 00:00:00
abstract:AIMS/HYPOTHESIS:Type 2 diabetes is characterised by excessive hepatic glucose production and frequently leads to systemic vascular complications. We therefore analysed the relationship between the gene expression profile in the liver and the pathophysiology of Type 2 diabetes. METHODS:Liver biopsy samples were obtaine...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s00125-004-1366-y
更新日期:2004-04-01 00:00:00
abstract:AIMS/HYPOTHESIS:It is unclear whether type 1 diabetes is a single disease or if endotypes exist. Our aim was to use a unique collection of pancreas samples recovered soon after disease onset to resolve this issue. METHODS:Immunohistological analysis was used to determine the distribution of proinsulin and insulin in t...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s00125-020-05115-6
更新日期:2020-06-01 00:00:00
abstract:AIMS/HYPOTHESIS:HLA haplotypes DRB1*03_DQB1*02 and DRB1*04_DQB1*0302, and allelic variation of the T cell regulatory gene cytotoxic T-lymphocyte-associated antigen-4 (CTLA4) and of the T cell activation gene protein tyrosine phosphatase, non-receptor type 22 (lymphoid) (PTPN22) have been associated with type 1 diabetes...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s00125-007-0603-6
更新日期:2007-04-01 00:00:00
abstract:AIMS/HYPOTHESIS:Diabetic cardiomyopathy (DCM) is associated with suppressed autophagy and augmented apoptosis in the heart although the interplay between the two remains elusive. The ability of mammalian sterile 20-like kinase 1 to regulate both autophagy and apoptosis prompted us to investigate it as a possible candid...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s00125-016-4070-9
更新日期:2016-11-01 00:00:00
abstract:AIMS/HYPOTHESIS:Cardiovascular events and death are better predicted by postprandial glucose (PPG) than by fasting blood glucose or HbA(1c). While chronic exercise reduces HbA(1c) in patients with type 2 diabetes, short-term exercise improves measures of insulin sensitivity but does not consistently alter responses to ...
journal_title:Diabetologia
pub_type: 临床试验,杂志文章
doi:10.1007/s00125-012-2490-8
更新日期:2012-05-01 00:00:00
abstract:AIMS/HYPOTHESIS:Pharmacological augmentation of glucagon-like peptide 1 receptor signalling by dipeptidyl peptidase 4 (DPP-4) inhibition reduced intestinal lipoprotein secretion in experimental studies, suggesting that DPP-4 inhibitors may ameliorate dyslipidaemia and thus reduce cardiovascular risk in patients with ty...
journal_title:Diabetologia
pub_type: 杂志文章,多中心研究,随机对照试验
doi:10.1007/s00125-011-2447-3
更新日期:2012-04-01 00:00:00
abstract:AIMS/HYPOTHESIS:Levels of ideal cardiovascular health (ICH) and incident type 2 diabetes mellitus have not been examined in a multiethnic population. We assessed the total and race/ethnicity-specific incidence of diabetes based on American Heart Association (AHA) ICH components. METHODS:Incident diabetes was assessed ...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s00125-016-4003-7
更新日期:2016-09-01 00:00:00
abstract:AIMS/HYPOTHESIS:The paucity of information on the epigenetic barriers that are blocking reprogramming protocols, and on what makes a beta cell unique, has hampered efforts to develop novel beta cell sources. Here, we aimed to identify enhancers in pancreatic islets, to understand their developmental ontologies, and to ...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s00125-012-2797-5
更新日期:2013-03-01 00:00:00
abstract:AIMS/HYPOTHESIS:No previous studies on the association between salt intake and the risk of type 2 diabetes have been reported. The aim of this study was to assess whether high salt intake, measured by 24-h urinary sodium excretion, is an independent risk factor for type 2 diabetes. METHODS:We followed prospectively 93...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s00125-005-1824-1
更新日期:2005-08-01 00:00:00
abstract:AIMS/HYPOTHESIS:Uncoupling protein-2 (UCP2) is thought to play a role in insulin secretion and the development of obesity. In this study, we investigated the effects of genetic variation in UCP2 on type 2 diabetes and obesity, as well as on metabolic phenotypes related to these diseases, in Pima Indians. METHODS:The c...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s00125-005-1934-9
更新日期:2005-11-01 00:00:00
abstract::Factor VIII-related antigen and von Willebrand factor are synthesised by and released from vascular endothelium. Acute increases in the plasma concentration of these proteins may reflect endothelial cell damage. We have thus measured the plasma concentration of factor VIII-related antigen and von Willebrand factor, to...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/BF00274221
更新日期:1987-03-01 00:00:00
abstract:AIMS/HYPOTHESIS:The aim of this work was to estimate the impact of birthweight on early-onset (age <40 years) type 2 diabetes. METHODS:A longitudinal study of American Indians, aged ≥5 years, was conducted from 1965 to 2007. Participants who had a recorded birthweight were followed until they developed diabetes or the...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s00125-019-4899-9
更新日期:2019-09-01 00:00:00
abstract::The incorporation of [35S]sulfate into heart proteoglycans has been studied in normal and alloxan-diabetic rats by perfusion and in vivo administration of the isotope; in the latter situation, comparison was also made of radiolabeled sulfate utilization by several other tissues (kidney, liver, lung, muscle, testes and...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/BF00270425
更新日期:1987-04-01 00:00:00
abstract::Screening and diagnostic criteria for gestational diabetes (GDM) are inconsistent across Europe, and the development of a uniform GDM screening strategy is necessary. Such a strategy would create opportunities for more women to receive timely treatment for GDM. Developing a consensus on screening for GDM in Europe is ...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s00125-015-3615-7
更新日期:2015-07-01 00:00:00
abstract:AIMS/HYPOTHESIS:In the double-blind placebo-controlled Fenofibrate Intervention and Event Lowering in Diabetes trial (n = 9,795), fenofibrate reduced major cardiovascular events in type 2 diabetes. Sex-related differences in fenofibrate response could be clinically relevant and were pre-specified analyses. METHODS:Wom...
journal_title:Diabetologia
pub_type: 杂志文章,随机对照试验
doi:10.1007/s00125-014-3344-3
更新日期:2014-11-01 00:00:00
abstract::We examined glomerular basement membrane anionic site distribution identified by cationic gold in seven patients with insulin-dependent and four patients with non-insulin-dependent diabetes mellitus, presenting a spectrum of clinical and glomerular changes. Anionic sites were investigated by pretreatment of tissue wit...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/BF00400607
更新日期:1995-12-01 00:00:00
abstract:AIMS/HYPOTHESIS:Genome-wide association studies (GWAS) for type 2 diabetes have uncovered >400 risk loci, primarily in populations of European and Asian ancestry. Here, we aimed to discover additional type 2 diabetes risk loci (including African-specific variants) and fine-map association signals by performing genetic ...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s00125-019-4880-7
更新日期:2019-07-01 00:00:00
abstract::Use of monoclonal antibodies specific for rat lymphocyte subsets and an anti-insulin marker has allowed us to document the following sequence of events leading to the development of clinical diabetes in this animal model. The first change observed in the pancreas is increased expression of MHC class II molecules on va...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/BF00280892
更新日期:1985-07-01 00:00:00
abstract::The accumulation of noradrenaline in constricted sciatic nerves was measured in 6 month diabetic rats (streptozotocin 35 mg/kg) and 4 day diabetic rats (streptozotocin 70 mg/kg) together with two groups of age-matched control animals. There was no alteration in the amount of noradrenaline accumulated in the nerves of ...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/BF00283753
更新日期:1982-03-01 00:00:00