Reduction of oxidative stress by oral N-acetyl-L-cysteine treatment decreases plasma soluble vascular cell adhesion molecule-1 concentrations in non-obese, non-dyslipidaemic, normotensive, patients with non-insulin-dependent diabetes.

Abstract:

:To assess in vivo effects of antioxidants on vascular cell adhesion molecule (VCAM)-1 expression, circulating soluble VCAM-1 and intraerythrocytic reduced glutathione (GSH) and GSH disulphide (GSSG) concentrations were evaluated in non-insulin-dependent diabetic patients without complications (9 men, 6 women, 48 +/- 6 years old) before and after 1 month of either oral N-acetyl-L-cysteine (1.200 mg/day) or placebo treatments, given in randomized, cross-over, double-blind fashion. Ten healthy subjects (7 men, 3 women, 52 +/- 4 years old) served as control subjects. Baseline plasma VCAM-1 concentrations were higher (p = 0.007) in non-insulin-dependent diabetic patients (707.9 +/- 52.5 ng/ml) than in control subjects (627.3 +/- 84.6 ng/ml). Intraerythrocytic GSSG content was higher (non-insulin dependent diabetic patients: 0.618 +/- 0.185 micromol/g Hb; control subjects: 0.352 +/- 0.04 micromol/g Hb, p = 0.0002), whereas intraerythrocytic GSH concentrations were lower (p = 0.001) in non-insulin dependent diabetic patients (6.0 +/- 0.7 micromol/g Hb) than in control subjects (7.1 +/- 0.5 micromol/g Hb). The mean GSH:GSSG ratio was also lower (p = 0.0001) in the first (10.9 +/- 4.5) than in the second group (20.2 +/- 1.4). Circulating VCAM-1 and intraerythrocytic GSH concentrations were negatively correlated in non-insulin diabetic patients (r = 0.605, p = 0.01). Treatment with N-acetyl-L-cysteine decreased plasma VCAM-1 (p = 0.01) and intraerythrocytic GSSG (p = 0.006) but increased GSH concentrations (p = 0.04) and the GSH:GSSG ratio (p = 0.004) in non-insulin dependent diabetic patients. Our data indicate that the vascular endothelium is activated in non-insulin dependent diabetes. Antioxidant treatment counterbalanced such endothelial activation. Thus, antioxidant agents might protect against oxidant-related upregulation of endothelial adhesion molecules and slow down the progression of vascular damage in non-insulin dependent diabetes.

journal_name

Diabetologia

journal_title

Diabetologia

authors

De Mattia G,Bravi MC,Laurenti O,Cassone-Faldetta M,Proietti A,De Luca O,Armiento A,Ferri C

doi

10.1007/s001250051082

subject

Has Abstract

pub_date

1998-11-01 00:00:00

pages

1392-6

issue

11

eissn

0012-186X

issn

1432-0428

journal_volume

41

pub_type

临床试验,杂志文章,随机对照试验
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    abstract:AIMS/HYPOTHESIS:Pancreatic islet transplantation (PIT) offers a physiological treatment for type 1 diabetes, but the failure of islet engraftment hinders its application. The female hormone 17β-oestradiol (E2) favours islet survival and stimulates angiogenesis, raising the possibility that E2 may enhance islet engraftm...

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    doi:10.1007/s00125-012-2764-1

    authors: Liu S,Kilic G,Meyers MS,Navarro G,Wang Y,Oberholzer J,Mauvais-Jarvis F

    更新日期:2013-02-01 00:00:00

  • Interleukin 1 dose-dependently affects the biosynthesis of (pro)insulin in isolated rat islets of Langerhans.

    abstract::Human crude and recombinant interleukin 1 (IL-1) was found to dose- and time-dependently affect the biosynthesis of (pro)insulin in isolated rat islets of Langerhans. Incubation of rat islets with either 0.5 U/ml or 5 U/ml of crude IL-1 for 1 h had no detectable effect on (pro)insulin biosynthesis. After 24 hours of e...

    journal_title:Diabetologia

    pub_type: 杂志文章

    doi:10.1007/BF00279615

    authors: Spinas GA,Hansen BS,Linde S,Kastern W,Mølvig J,Mandrup-Poulsen T,Dinarello CA,Nielsen JH,Nerup J

    更新日期:1987-07-01 00:00:00

  • Leptin resistance exacerbates diet-induced obesity and is associated with diminished maximal leptin signalling capacity in rats.

    abstract:AIMS/HYPOTHESIS:Leptin resistance is generally considered a consequence of obesity. We postulated that leptin resistance is associated with diminished hypothalamic leptin signalling capacity and that leptin resistance is causal to obesity. We assessed maximal leptin-mediated binding of the transcription factor signal t...

    journal_title:Diabetologia

    pub_type: 杂志文章

    doi:10.1007/s00125-005-1763-x

    authors: Scarpace PJ,Matheny M,Tümer N,Cheng KY,Zhang Y

    更新日期:2005-06-01 00:00:00

  • Down regulation of peroxisome proliferator-activated receptorgamma expression by inflammatory cytokines and its reversal by thiazolidinediones.

    abstract:AIMS/HYPOTHESIS:Previous studies show that inflammatory cytokines play a part in the development of insulin resistance. Thiazolidinediones were developed as insulin-sensitizing drugs and are ligands for the peroxisome proliferator-activated receptory (PPARgamma). We hypothesized that the anti-diabetic mechanism of thia...

    journal_title:Diabetologia

    pub_type: 杂志文章

    doi:10.1007/s001250051218

    authors: Tanaka T,Itoh H,Doi K,Fukunaga Y,Hosoda K,Shintani M,Yamashita J,Chun TH,Inoue M,Masatsugu K,Sawada N,Saito T,Inoue G,Nishimura H,Yoshimasa Y,Nakao K

    更新日期:1999-06-01 00:00:00

  • Detection of IgE insulin antibody with radioallergosorbent test.

    abstract::An in vitro method for detecting IgE insulin antibody using the principle of the radioallergosorbent test (RAST) is described. In six patients with insulin allergy, the RAST values were higher than in normal persons or insulin-treated diabetics without insulin-allergy. No differences were observed between normal perso...

    journal_title:Diabetologia

    pub_type: 杂志文章

    doi:10.1007/BF00429705

    authors: Nakagawa S,Saito N,Nakayama H,Sasaki T,Watanabe T,Aoki S

    更新日期:1978-01-14 00:00:00

  • 15th Golgi lecture: from hyperglycaemia to the dysregulation of vascular remodelling in diabetes.

    abstract::Hyperglycaemia has been shown to play a central part in diabetic vascular disease, which is also influenced by individual background. Hyperglycaemia initiates the pathogenetic sequence through a series of interrelated biochemical abnormalities, including increased flux through the polyol and hexosamine pathways, oxida...

    journal_title:Diabetologia

    pub_type:

    doi:10.1007/s001250051676

    authors: Di Mario U,Pugliese G

    更新日期:2001-06-01 00:00:00

  • Non-esterified fatty acids and the liver: why is insulin secreted into the portal vein?

    abstract:AIMS/HYPOTHESIS:Insulin control of glucose output is a major mechanism by which appropriate amounts of glucose are produced to supply energy to the central nervous system, without causing long-term increases of the plasma glucose concentration. It is hypothesised that the primary route by which insulin maintains contro...

    journal_title:Diabetologia

    pub_type: 杂志文章,评审

    doi:10.1007/s001250051474

    authors: Bergman RN

    更新日期:2000-07-01 00:00:00

  • Enhanced VEGF signalling mediates cerebral neovascularisation via downregulation of guidance protein ROBO4 in a rat model of diabetes.

    abstract:AIMS/HYPOTHESIS:Diabetes promotes cerebral neovascularisation via increased vascular endothelial growth factor (VEGF) angiogenic signalling. Roundabout-4 (ROBO4) protein is an endogenous inhibitor of VEGF signalling that stabilises the vasculature. Yet, how diabetes affects ROBO4 function remains unknown. We hypothesis...

    journal_title:Diabetologia

    pub_type: 杂志文章

    doi:10.1007/s00125-017-4214-6

    authors: Abdelsaid M,Coucha M,Hafez S,Yasir A,Johnson MH,Ergul A

    更新日期:2017-04-01 00:00:00

  • Transgenic overexpression of brain natriuretic peptide prevents the progression of diabetic nephropathy in mice.

    abstract:AIMS/HYPOTHESIS:Brain natriuretic peptide (BNP) is a potent vasorelaxing and natriuretic peptide that is secreted from the heart and has cardioprotective properties. We have previously generated hypotensive transgenic mice (BNP-Tg mice) that overproduce BNP in the liver, which is released into the circulation. Using th...

    journal_title:Diabetologia

    pub_type: 杂志文章

    doi:10.1007/s00125-006-0352-y

    authors: Makino H,Mukoyama M,Mori K,Suganami T,Kasahara M,Yahata K,Nagae T,Yokoi H,Sawai K,Ogawa Y,Suga S,Yoshimasa Y,Sugawara A,Tanaka I,Nakao K

    更新日期:2006-10-01 00:00:00

  • IGF-1 and cardiometabolic diseases: a Mendelian randomisation study.

    abstract:AIMS/HYPOTHESIS:Abnormal serum IGF-1 levels are associated with an increased risk of type 2 diabetes and cardiovascular disease. However, the causal role of IGF-1 levels within the normal range in cardiometabolic disease remains unclear. We employed Mendelian randomisation to explore the associations between geneticall...

    journal_title:Diabetologia

    pub_type: 杂志文章

    doi:10.1007/s00125-020-05190-9

    authors: Larsson SC,Michaëlsson K,Burgess S

    更新日期:2020-09-01 00:00:00

  • Genetic epidemiology of MODY in the Czech republic: new mutations in the MODY genes HNF-4alpha, GCK and HNF-1alpha.

    abstract:AIMS/HYPOTHESIS:The aim of this study was to examine the prevalence and nature of mutations in HNF4alpha/MODY1, GCK/MODY2 and HNF-1alpha/MODY3 genes in Czech subjects with clinical diagnosis of MODY. METHODS:We studied 61 unrelated index probands of Czech origin (28 males, 33 females) with a clinical diagnosis of MODY...

    journal_title:Diabetologia

    pub_type: 杂志文章

    doi:10.1007/s00125-002-1010-7

    authors: Pruhova S,Ek J,Lebl J,Sumnik Z,Saudek F,Andel M,Pedersen O,Hansen T

    更新日期:2003-02-01 00:00:00

  • Genes for systemic vascular complications are differentially expressed in the livers of type 2 diabetic patients.

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    journal_title:Diabetologia

    pub_type: 杂志文章

    doi:10.1007/s00125-004-1366-y

    authors: Takamura T,Sakurai M,Ota T,Ando H,Honda M,Kaneko S

    更新日期:2004-04-01 00:00:00

  • Studies of insulin and proinsulin in pancreas and serum support the existence of aetiopathological endotypes of type 1 diabetes associated with age at diagnosis.

    abstract:AIMS/HYPOTHESIS:It is unclear whether type 1 diabetes is a single disease or if endotypes exist. Our aim was to use a unique collection of pancreas samples recovered soon after disease onset to resolve this issue. METHODS:Immunohistological analysis was used to determine the distribution of proinsulin and insulin in t...

    journal_title:Diabetologia

    pub_type: 杂志文章

    doi:10.1007/s00125-020-05115-6

    authors: Leete P,Oram RA,McDonald TJ,Shields BM,Ziller C,TIGI study team.,Hattersley AT,Richardson SJ,Morgan NG

    更新日期:2020-06-01 00:00:00

  • A type 1 diabetes subgroup with a female bias is characterised by failure in tolerance to thyroid peroxidase at an early age and a strong association with the cytotoxic T-lymphocyte-associated antigen-4 gene.

    abstract:AIMS/HYPOTHESIS:HLA haplotypes DRB1*03_DQB1*02 and DRB1*04_DQB1*0302, and allelic variation of the T cell regulatory gene cytotoxic T-lymphocyte-associated antigen-4 (CTLA4) and of the T cell activation gene protein tyrosine phosphatase, non-receptor type 22 (lymphoid) (PTPN22) have been associated with type 1 diabetes...

    journal_title:Diabetologia

    pub_type: 杂志文章

    doi:10.1007/s00125-007-0603-6

    authors: Howson JM,Dunger DB,Nutland S,Stevens H,Wicker LS,Todd JA

    更新日期:2007-04-01 00:00:00

  • MST1 coordinately regulates autophagy and apoptosis in diabetic cardiomyopathy in mice.

    abstract:AIMS/HYPOTHESIS:Diabetic cardiomyopathy (DCM) is associated with suppressed autophagy and augmented apoptosis in the heart although the interplay between the two remains elusive. The ability of mammalian sterile 20-like kinase 1 to regulate both autophagy and apoptosis prompted us to investigate it as a possible candid...

    journal_title:Diabetologia

    pub_type: 杂志文章

    doi:10.1007/s00125-016-4070-9

    authors: Zhang M,Zhang L,Hu J,Lin J,Wang T,Duan Y,Man W,Feng J,Sun L,Jia H,Li C,Zhang R,Wang H,Sun D

    更新日期:2016-11-01 00:00:00

  • Glycaemic control is improved by 7 days of aerobic exercise training in patients with type 2 diabetes.

    abstract:AIMS/HYPOTHESIS:Cardiovascular events and death are better predicted by postprandial glucose (PPG) than by fasting blood glucose or HbA(1c). While chronic exercise reduces HbA(1c) in patients with type 2 diabetes, short-term exercise improves measures of insulin sensitivity but does not consistently alter responses to ...

    journal_title:Diabetologia

    pub_type: 临床试验,杂志文章

    doi:10.1007/s00125-012-2490-8

    authors: Mikus CR,Oberlin DJ,Libla J,Boyle LJ,Thyfault JP

    更新日期:2012-05-01 00:00:00

  • Lowering of postprandial lipids in individuals with type 2 diabetes treated with alogliptin and/or pioglitazone: a randomised double-blind placebo-controlled study.

    abstract:AIMS/HYPOTHESIS:Pharmacological augmentation of glucagon-like peptide 1 receptor signalling by dipeptidyl peptidase 4 (DPP-4) inhibition reduced intestinal lipoprotein secretion in experimental studies, suggesting that DPP-4 inhibitors may ameliorate dyslipidaemia and thus reduce cardiovascular risk in patients with ty...

    journal_title:Diabetologia

    pub_type: 杂志文章,多中心研究,随机对照试验

    doi:10.1007/s00125-011-2447-3

    authors: Eliasson B,Möller-Goede D,Eeg-Olofsson K,Wilson C,Cederholm J,Fleck P,Diamant M,Taskinen MR,Smith U

    更新日期:2012-04-01 00:00:00

  • The association of ideal cardiovascular health with incident type 2 diabetes mellitus: the Multi-Ethnic Study of Atherosclerosis.

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    journal_title:Diabetologia

    pub_type: 杂志文章

    doi:10.1007/s00125-016-4003-7

    authors: Joseph JJ,Echouffo-Tcheugui JB,Carnethon MR,Bertoni AG,Shay CM,Ahmed HM,Blumenthal RS,Cushman M,Golden SH

    更新日期:2016-09-01 00:00:00

  • Identification and analysis of murine pancreatic islet enhancers.

    abstract:AIMS/HYPOTHESIS:The paucity of information on the epigenetic barriers that are blocking reprogramming protocols, and on what makes a beta cell unique, has hampered efforts to develop novel beta cell sources. Here, we aimed to identify enhancers in pancreatic islets, to understand their developmental ontologies, and to ...

    journal_title:Diabetologia

    pub_type: 杂志文章

    doi:10.1007/s00125-012-2797-5

    authors: Tennant BR,Robertson AG,Kramer M,Li L,Zhang X,Beach M,Thiessen N,Chiu R,Mungall K,Whiting CJ,Sabatini PV,Kim A,Gottardo R,Marra MA,Lynn FC,Jones SJ,Hoodless PA,Hoffman BG

    更新日期:2013-03-01 00:00:00

  • Urinary sodium and potassium excretion and the risk of type 2 diabetes: a prospective study in Finland.

    abstract:AIMS/HYPOTHESIS:No previous studies on the association between salt intake and the risk of type 2 diabetes have been reported. The aim of this study was to assess whether high salt intake, measured by 24-h urinary sodium excretion, is an independent risk factor for type 2 diabetes. METHODS:We followed prospectively 93...

    journal_title:Diabetologia

    pub_type: 杂志文章

    doi:10.1007/s00125-005-1824-1

    authors: Hu G,Jousilahti P,Peltonen M,Lindström J,Tuomilehto J

    更新日期:2005-08-01 00:00:00

  • Genetic variation in UCP2 (uncoupling protein-2) is associated with energy metabolism in Pima Indians.

    abstract:AIMS/HYPOTHESIS:Uncoupling protein-2 (UCP2) is thought to play a role in insulin secretion and the development of obesity. In this study, we investigated the effects of genetic variation in UCP2 on type 2 diabetes and obesity, as well as on metabolic phenotypes related to these diseases, in Pima Indians. METHODS:The c...

    journal_title:Diabetologia

    pub_type: 杂志文章

    doi:10.1007/s00125-005-1934-9

    authors: Kovacs P,Ma L,Hanson RL,Franks P,Stumvoll M,Bogardus C,Baier LJ

    更新日期:2005-11-01 00:00:00

  • Changes in the factor VIII complex in diabetic ketoacidosis: evidence of endothelial cell damage?

    abstract::Factor VIII-related antigen and von Willebrand factor are synthesised by and released from vascular endothelium. Acute increases in the plasma concentration of these proteins may reflect endothelial cell damage. We have thus measured the plasma concentration of factor VIII-related antigen and von Willebrand factor, to...

    journal_title:Diabetologia

    pub_type: 杂志文章

    doi:10.1007/BF00274221

    authors: Greaves M,Pickering C,Knight G,Boulton AJ,Ball J,Ward JD,Preston FE

    更新日期:1987-03-01 00:00:00

  • Birthweight and early-onset type 2 diabetes in American Indians: differential effects in adolescents and young adults and additive effects of genotype, BMI and maternal diabetes.

    abstract:AIMS/HYPOTHESIS:The aim of this work was to estimate the impact of birthweight on early-onset (age <40 years) type 2 diabetes. METHODS:A longitudinal study of American Indians, aged ≥5 years, was conducted from 1965 to 2007. Participants who had a recorded birthweight were followed until they developed diabetes or the...

    journal_title:Diabetologia

    pub_type: 杂志文章

    doi:10.1007/s00125-019-4899-9

    authors: Olaiya MT,Wedekind LE,Hanson RL,Sinha M,Kobes S,Nelson RG,Baier LJ,Knowler WC

    更新日期:2019-09-01 00:00:00

  • Sulfate metabolism in the alloxan-diabetic rat: relationship of altered sulfate pools to proteoglycan sulfation in heart and other tissues.

    abstract::The incorporation of [35S]sulfate into heart proteoglycans has been studied in normal and alloxan-diabetic rats by perfusion and in vivo administration of the isotope; in the latter situation, comparison was also made of radiolabeled sulfate utilization by several other tissues (kidney, liver, lung, muscle, testes and...

    journal_title:Diabetologia

    pub_type: 杂志文章

    doi:10.1007/BF00270425

    authors: Spiro MJ

    更新日期:1987-04-01 00:00:00

  • A proposal for the use of uniform diagnostic criteria for gestational diabetes in Europe: an opinion paper by the European Board & College of Obstetrics and Gynaecology (EBCOG).

    abstract::Screening and diagnostic criteria for gestational diabetes (GDM) are inconsistent across Europe, and the development of a uniform GDM screening strategy is necessary. Such a strategy would create opportunities for more women to receive timely treatment for GDM. Developing a consensus on screening for GDM in Europe is ...

    journal_title:Diabetologia

    pub_type: 杂志文章

    doi:10.1007/s00125-015-3615-7

    authors: Benhalima K,Mathieu C,Damm P,Van Assche A,Devlieger R,Desoye G,Corcoy R,Mahmood T,Nizard J,Savona-Ventura C,Dunne F

    更新日期:2015-07-01 00:00:00

  • Favourable effects of fenofibrate on lipids and cardiovascular disease in women with type 2 diabetes: results from the Fenofibrate Intervention and Event Lowering in Diabetes (FIELD) study.

    abstract:AIMS/HYPOTHESIS:In the double-blind placebo-controlled Fenofibrate Intervention and Event Lowering in Diabetes trial (n = 9,795), fenofibrate reduced major cardiovascular events in type 2 diabetes. Sex-related differences in fenofibrate response could be clinically relevant and were pre-specified analyses. METHODS:Wom...

    journal_title:Diabetologia

    pub_type: 杂志文章,随机对照试验

    doi:10.1007/s00125-014-3344-3

    authors: d'Emden MC,Jenkins AJ,Li L,Zannino D,Mann KP,Best JD,Stuckey BG,Park K,Saltevo J,Keech AC,FIELD Study Investigators.

    更新日期:2014-11-01 00:00:00

  • Alterations of glomerular basement membrane charge and structure in diabetic nephropathy.

    abstract::We examined glomerular basement membrane anionic site distribution identified by cationic gold in seven patients with insulin-dependent and four patients with non-insulin-dependent diabetes mellitus, presenting a spectrum of clinical and glomerular changes. Anionic sites were investigated by pretreatment of tissue wit...

    journal_title:Diabetologia

    pub_type: 杂志文章

    doi:10.1007/BF00400607

    authors: Goode NP,Shires M,Crellin DM,Aparicio SR,Davison AM

    更新日期:1995-12-01 00:00:00

  • Genome-wide association study of type 2 diabetes in Africa.

    abstract:AIMS/HYPOTHESIS:Genome-wide association studies (GWAS) for type 2 diabetes have uncovered >400 risk loci, primarily in populations of European and Asian ancestry. Here, we aimed to discover additional type 2 diabetes risk loci (including African-specific variants) and fine-map association signals by performing genetic ...

    journal_title:Diabetologia

    pub_type: 杂志文章

    doi:10.1007/s00125-019-4880-7

    authors: Chen J,Sun M,Adeyemo A,Pirie F,Carstensen T,Pomilla C,Doumatey AP,Chen G,Young EH,Sandhu M,Morris AP,Barroso I,McCarthy MI,Mahajan A,Wheeler E,Rotimi CN,Motala AA

    更新日期:2019-07-01 00:00:00

  • Pre-diabetes in the spontaneously diabetic BB/E rat: lymphocyte subpopulations in the pancreatic infiltrate and expression of rat MHC class II molecules in endocrine cells.

    abstract::Use of monoclonal antibodies specific for rat lymphocyte subsets and an anti-insulin marker has allowed us to document the following sequence of events leading to the development of clinical diabetes in this animal model. The first change observed in the pancreas is increased expression of MHC class II molecules on va...

    journal_title:Diabetologia

    pub_type: 杂志文章

    doi:10.1007/BF00280892

    authors: Dean BM,Walker R,Bone AJ,Baird JD,Cooke A

    更新日期:1985-07-01 00:00:00

  • Axonal transport of noradrenaline and noradrenergic transmission in rats with streptozotocin-induced diabetes.

    abstract::The accumulation of noradrenaline in constricted sciatic nerves was measured in 6 month diabetic rats (streptozotocin 35 mg/kg) and 4 day diabetic rats (streptozotocin 70 mg/kg) together with two groups of age-matched control animals. There was no alteration in the amount of noradrenaline accumulated in the nerves of ...

    journal_title:Diabetologia

    pub_type: 杂志文章

    doi:10.1007/BF00283753

    authors: Tomlinson DR,Gillon KR,Smith MG

    更新日期:1982-03-01 00:00:00