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Identification of H. pylori in saliva by a nested PCR assay derived from a newly cloned DNA probe.

Abstract：

:A novel probe was developed from genomic DNA of Helicobacter pylori ATCC type strain 43629. It hybridized with all 73 H. pylori clinical isolates tested but not with any of 183 non-H. pylori DNAs in dot blot hybridization. Typing tests revealed 41 different HaeIII-digestion patterns from 57 H. pylori strains tested. Based on the sequence of the probe, a nested PCR was developed that detected as little as 2 fg of H. pylori DNA or approximately equivalent to one cell. No PCR products were amplified from any of 21 non-H. pylori strains tested. Using this nested PCR, H. pylori DNA was detected in 33 of 45 (73%) saliva samples collected from patients with gastric H. pylori infection. These data suggest that the probe is useful for typing H. pylori and that the nested PCR is a valuable tool for detecting H. pylori DNA in saliva.

journal_name

Dig Dis Sci

journal_title

Digestive diseases and sciences

authors

Jiang C,Li C,Ha T,Ferguson DA Jr,Chi DS,Laffan JJ,Thomas E

doi

10.1023/a:1018847522200

subject

Has Abstract

pub_date

1998-06-01 00:00:00

pages

1211-8

issue

6

eissn

0163-2116

issn

1573-2568

journal_volume

43

pub_type

杂志文章

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