"Cut and combine": an easy membrane-supported combinatorial synthesis technique.

Abstract:

:A combinatorial synthesis process involving sequential cycles of cutting a membrane support into pieces and combining these into groups and subjecting the groups to simultaneous solid-phase chemical reactions is demonstrated by the rapid assembly of four hundred N-terminally biotinylated, soluble, octameric peptide pools. Index patterns printed onto the synthesis membrane allowed a direct identification of the compounds. These were used to study protein kinase substrate selection in a parallel microplate adapted 32P-phosphorylation assay with subsequent spotting on a biotin-capture membrane.

journal_name

Bioorg Med Chem Lett

authors

Dittrich F,Tegge W,Frank R

doi

10.1016/s0960-894x(98)00425-9

subject

Has Abstract

pub_date

1998-09-08 00:00:00

pages

2351-6

issue

17

eissn

0960-894X

issn

1464-3405

pii

S0960894X98004259

journal_volume

8

pub_type

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