A new high resolution radioimager for the quantitative analysis of radiolabelled molecules in tissue section.

Abstract:

:We present a high-speed, high-resolution imager of beta particles. It is devoted to be used in autoradiography experiments such as receptor binding or in situ hybridization experiments, either instead of, or in complement with autoradiographic film and emulsions. It allows the user to locate and perform quantitative analyses of (3H, 14C, 35S, 33P, 32P, 125I) labelled molecules with a 15 microm spatial resolution on a 0.9 x 1.3 cm2 sensitive area. Combining recent techniques (specific scintillator thin sheets and intensified charge-coupled device (CCD)) this imager offers a wide dynamic range and real-time acquisition.

journal_name

J Neurosci Methods

authors

Laniece P,Charon Y,Cardona A,Pinot L,Maitrejean S,Mastrippolito R,Sandkamp B,Valentin L

doi

10.1016/s0165-0270(98)00128-9

subject

Has Abstract

pub_date

1998-12-31 00:00:00

pages

1-5

issue

1

eissn

0165-0270

issn

1872-678X

pii

S0165027098001289

journal_volume

86

pub_type

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