IL-6 antisense oligonucleotides inhibit IgE production in IL-4 and anti-CD40-stimulated human B-lymphocytes.

Abstract:

:Stimulation of tonsillar B-lymphocytes with CD40 antibodies and IL-4 leads to homotypic adhesion, proliferation, and differentiation into Ig-producing cells. It also leads to the production of IL-6, a pleiotropic cytokine involved in B-cell maturation and differentiation. To assess the importance of IL-6 in the differentiation process, an antisense oligonucleotide to IL-6 was added to tonsillar B-cells together with CD40 antibodies and IL-4. This led to clearly reduced levels of IL-6 as well as to a specific inhibition of IgE production. Also, IgG secretion was somewhat reduced while IgM appeared to be unaffected. The effects were not due to toxicity of the oligonucleotide since proliferation proceeded normally or was slightly enhanced in the presence of the antisense. The findings show that endogenous IL-6 is an important co-factor for the generation of B-cells secreting IgE and IgG but that it is not required for IgM production. They further indicate that IL-6 may not be necessary as a co-factor in CD40/IL-4 induced proliferation.

journal_name

Immunol Lett

journal_title

Immunology letters

authors

Björck P,Larsson S,Andäng M,Ahrlund-Richter L,Paulie S

doi

10.1016/s0165-2478(97)00140-5

subject

Has Abstract

pub_date

1998-03-01 00:00:00

pages

1-5

issue

1

eissn

0165-2478

issn

1879-0542

pii

S0165-2478(97)00140-5

journal_volume

61

pub_type

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