Abstract：
:The experimental evidence linking glutamate to ischemic neuronal injury is derived from in vitro or in vivo animal stroke models. We, therefore, developed an in vitro preparation to determine whether glutamate contributes to early neuronal swelling in oxygen and glucose deprived (OGD) human neocortical slices. In order to monitor neuronal swelling, we measured extracellular tissue resistance in brain slices by passing constant current pulses through two electrodes and recording the voltage drop between them. We verified that NMDA (30 microM) or OGD induced a rise in tissue resistance in rat neocortical slices. We then examined human neocortical slices from 11 patients undergoing resections for intractable epilepsy. Both the rodent and human neocortical slices swelled within 10 min of OGD. In both, the glutamate antagonist dizocilpine (MK-801) reduced the swelling. In the rats, MK-801 (5 microM) prolonged the latency to onset of neuronal swelling following OGD from 7.6 +/- 0.6 min (mean +/- S.E.M., n = 16) to 17.4 +/- 2.6 min (n = 6; p < 0.01). Other putative neuroprotective agents were much less effective in this paradigm. In the human slices, MK-801 again prolonged the latency to resistance increase from 8.6 +/- 0.4 min (n = 8) to 17.2 +/- 1.7 min (n = 9, p < 0.01). This is the direct demonstration that glutamate receptor activation leads to neuronal swelling in substrate deficient human brain. These results, which are similar to those obtained in the rodent brain slices, help validate the animal slices as appropriate models for the study of OGD in human brain.
journal_name
Brain Resjournal_title
Brain researchauthors
Werth JL,Park TS,Silbergeld DL,Rothman SMdoi
10.1016/s0006-8993(97)01286-9subject
Has Abstractpub_date
1998-01-26 00:00:00pages
248-54issue
1-2eissn
0006-8993issn
1872-6240pii
S0006-8993(97)01286-9journal_volume
782pub_type
杂志文章相关文献
BRAIN RESEARCH文献大全abstract：:The influence of extracellular sinusoidal electric fields on the amplitude of population spikes evoked by single test pulses in excitatory pathways to CA1 pyramidal neurons was studied in rat hippocampal slices. The fields in the tissue were of the order of EEG gradients. Stimulation at 5 Hz, a frequency representativ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(84)90293-2
更新日期：1984-12-10 00:00:00
abstract：:Changes in GABA(A) receptor alpha(1) subunit gene expression occur in the globus pallidus and substantia nigra pars reticulata following lesions of the nigrostriatal tract. To determine whether these changes are translated at the protein level, we performed quantitative autoradiography with the alpha(1) selective liga...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(00)02081-3
更新日期：2000-04-17 00:00:00
abstract：:The incidence of amyotrophic lateral sclerosis (ALS) is higher among men than women but rises in women after the menopause. Estrogens may play a protective role. Treatment with estrogens has been shown to be neuroprotective in models of several neurodegenerative diseases. We therefore determined the effect of ovariect...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2004.06.024
更新日期：2004-09-17 00:00:00
abstract：:Adenine and guanine nucleotides have been shown to exert multiple roles in central and peripheral nervous systems, and the sequential breakdown of these nucleotides by enzymatic systems is an important step in the modulation of their extracellular effects. The aim of this study was to investigate whether nucleotide hy...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(02)02987-6
更新日期：2002-09-20 00:00:00
abstract：:The nucleus basalis of Meynert (nbM) was lesioned by injection of ibotenic acid, in 200 g male Wistar rats. The rats were killed 1, 3, 7 or 21 days after surgery, the brains were removed and the prefrontal cortices were subjected to immunohistochemical and Western blot analysis for the expression of glial fibrillary a...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(92)90800-o
更新日期：1992-03-06 00:00:00
abstract：:The activity of the septo-hippocampal and nucleus basalis-cortical cholinergic pathways was investigated by measuring changes in the extracellular acetylcholine levels in the hippocampus and parietal cortex, by means of transversal microdialysis, during the acquisition and recall of a positively reinforced operant beh...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(96)00292-2
更新日期：1996-06-10 00:00:00
abstract：:The effect of high concentrations of L-ascorbic acid on the in vivo and in vitro growth of human neuroblastoma has been investigated. Directly implemented into cell culture it decreased the DNA, RNA and protein synthesis, and mitosis of neuroblastoma cells, without affecting normal neuronal cells. In vivo treatment of...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(85)91139-4
更新日期：1985-09-09 00:00:00
abstract：:We examined the distribution of choline acetyltransferase (ChAT) mRNA in the brain of six autopsied individuals by in situ hybridization with 35S-labeled human ChAT riboprobes. Neurons containing hybridization signal for ChAT mRNA were observed in the nucleus of the diagonal band of Broca, the basal nucleus of Meynert...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(98)00677-5
更新日期：1998-09-21 00:00:00
abstract：:A new compound, 3-((+/-)-2-carboxypiperazin-4-yl)-propyl-1-phosphonic acid (CPP), has been evaluated as an excitatory amino acid receptor antagonist using electrophysiological assays and radioligand binding. In autoradiographic preparations, CPP reduces L-[3H]glutamate binding in regions of the hippocampus rich in N-m...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(86)90128-9
更新日期：1986-09-10 00:00:00
abstract：:The effects of substantia nigra (SN) stimulation on the activity of the nucleus ventralis lateralis of the thalamus (VL) have been studied. The VL cells were identified as relay or non-relay cells among the cerebellothalamocortical pathway on the basis of orthodromical activation from the cerebellum and antidromical a...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(78)90793-x
更新日期：1978-04-21 00:00:00
abstract：:Stimulation of spinal serotonin (5-HT)(2A/2C) receptors has previously been reported to lead to either a pro-nociceptive or an anti-nociceptive response. Behavioral data have indicated that the pro-nociceptive effect is related to the release of substance P (SP). The aim of this in vivo microdialysis study was to inve...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(03)03216-5
更新日期：2003-10-10 00:00:00
abstract：:Studies of the subcellular localization of neuroreceptors in the rat brain have shown that most of them are associated with light and low density subcellular fractions. In two human brain areas, quite different subcellular distributions were observed. After fractionation by differential centrifugation of frontal corte...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(95)00488-c
更新日期：1995-07-31 00:00:00
abstract：:How does attention interact with learning? Kruschke [Kruschke, J.K. (2001). Toward a unified Model of Attention in Associative Learning. J. Math. Psychol. 45, 812-863.] proposed a model (EXIT) that captures Mackintosh's [Mackintosh, N.J. (1975). A theory of attention: Variations in the associability of stimuli with re...
journal_title：Brain research
pub_type： 杂志文章,评审
doi：10.1016/j.brainres.2007.06.097
更新日期：2008-04-02 00:00:00
abstract：:The petrosal ganglia contain the somata of the sensory fibers of the glossopharyngeal nerves, innervating structures of the tongue, pharynx, carotid sinus and carotid body. Petrosal ganglia were excised from adult cats and their neurons were dissociated and kept in tissue culture for 7-12 days. Intracellular recording...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(00)02817-1
更新日期：2000-11-03 00:00:00
abstract：:Somatostatin receptors have been identified in different brain areas but the characterization of their postreceptor effect is still lacking. In this study we analyze the somatostatin effect on adenylate cyclase activity in different brain regions, namely frontal cortex, striatum, hypothalamus and hippocampus. Somatost...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(89)90889-5
更新日期：1989-07-17 00:00:00
abstract：:Ventilated, chloralose anesthetized cats were subjected to 3.2 atmosphere fluid percussion injury and compared with sham operated controls. In both groups, global cerebral blood flow (CBF), cerebral metabolic rate of oxygen utilization (CMRO2), cerebral metabolic rate of glucose utilization (CMRG1), and 31P magnetic r...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(92)91205-s
更新日期：1992-07-10 00:00:00
abstract：:Hypoxia-inducible transcription factors (HIFs) are crucially involved in brain development and cellular adaptation to hypoxia and ischemia. Degradation of HIF is regulated under normoxia by oxygen-dependent hydroxylation of specific prolyl residues on the labile alpha-subunit by HIF prolyl hydroxylases (PHD). Prolyl-4...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2009.05.023
更新日期：2009-07-14 00:00:00
abstract：:Natural food sources constitute a promising source of new compounds with neuroprotective properties, once they have the ability to reach the brain. Our aim was to evaluate the brain accessibility of quercetin, epigallocatechin gallate (EGCG) and cyanidin-3-glucoside (C3G) in relation to their neuroprotective capabilit...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2016.09.020
更新日期：2016-11-15 00:00:00
abstract：:Stroke is the major cause of acquired epilepsy in the adult population. The mechanisms of ischemia-induced epileptogenesis are not completely understood, but glutamate is associated with both ischemia-induced injury and epileptogenesis. The objective of this study was to develop an in vitro model of epileptogenesis in...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2010.11.065
更新日期：2011-01-31 00:00:00
abstract：:The effect of monocular enucleation on basophilic and metabolic staining in primary (striate) visual cortex has been compared in Old and New World monkeys. Both species show a 30-40% shrinkage of neurons in the layers of dorsal lateral geniculate nucleus receiving axons from the enucleated eye. In striate cortex Old W...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(85)91589-6
更新日期：1985-05-06 00:00:00
abstract：:Entopeduncular nucleus (EPN) cells which project to thalamic and non-thalamic sites were identified by antidromic discharge. Each population of cells, projecting to the ventral anterior nucleus of the thalamus (VA), centrum medianum (CM), the lateral habenula (LHB), and the pedunculopontine nucleus (PP), was distribut...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(78)90993-9
更新日期：1978-11-17 00:00:00
abstract：:The response properties of neurons in the region of striate cortex subserving central retina (0 degrees-2 degrees) and in a region of representation of parafoveal retina (4 degrees-7 degrees) were studied in unanesthetized paralyzed macaque monkeys. Neurons sensitive to the orientation of the stimulus in the visual fi...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(75)90240-1
更新日期：1975-12-12 00:00:00
abstract：:There is evidence that D1 and D2 dopamine receptor subtypes coexist at the cellular level in the striatum and act synergistically to mediate the effects of dopamine. Other data suggest that these receptor subtypes are largely segregated in different striatal projection pathways. We used in situ hybridization in serial...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(93)90303-5
更新日期：1993-09-03 00:00:00
abstract：:Immunohistochemical studies have suggested that corticotropin-releasing factor (CRF) was a transmitter of the olivocerebellar projection. We used in situ hybridization histochemistry with a 35S-labeled oligodeoxyribonucleotide probe for CRF mRNA to show that inferior olivary neurons of rats, baboons, and humans synthe...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0169-328x(86)90010-0
更新日期：1986-11-01 00:00:00
abstract：:Kappa opioid receptors (KORs) were immunocytochemically localized at four different levels of the spinal cord of normally-cycling female rats in estrus or diestrus. KOR labeling was primarily observed in fine processes and a few neuronal cell bodies in the superficial dorsal horn and the dorsolateral funiculus. Quanti...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(01)02515-x
更新日期：2001-06-29 00:00:00
abstract：:Deacetylation is a rapid clearance mechanism for ocular melatonin. We have studied the distribution of retinal melatonin deacetylase activity among vertebrate classes. Exogenous radiolabeled melatonin is metabolized by ocular tissue prepared from the amphibian Xenopus laevis, the reptile Anolis carolinensis, the teleo...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(91)90286-5
更新日期：1991-09-13 00:00:00
abstract：:The effects of selective axotomy of serotoninergic neurons produced by an intracerebroventricular injection of 5,7-dihydroxytryptamine (200 micrograms free base) on 5-HT1B binding sites labeled with S-CM-G-[125I]TNH2 were investigated by quantitative autoradiography in the rat brain. Results show, 21 days after surger...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(93)91452-x
更新日期：1993-10-01 00:00:00
abstract：:Neurointermediate lobes of rats comprise elements which, when excited in vitro, bring about an inhibition of the release of melanocyte stimulating hormone (MSH). Superfusion of neurointermediate lobes of intact donor rats with medium containing 45 mM K+ induced a stimulation of the release of oxytocin, arginine-vasopr...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(79)91047-3
更新日期：1979-08-10 00:00:00
abstract：:Horseradish peroxidase was injected intracellularly into motoneurons responding to cutaneous stimulation of the central foot pad of the hind limb in cats. Three motoneurons were selected for detailed analysis: two excited by foot pad stimulation, and one postsynaptically inhibited by such stimulation. The overall leng...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(82)90669-2
更新日期：1982-12-16 00:00:00
abstract：:Perfusion of artificial CSF from a lateral ventricle to the cerebral cortical subarachnoid space in anaesthetized cats was used to study the contribution of the cerebral cortex to the concentration of 5-hydroxyindoleacetic acid (5-HIAA) in CSF. The technique did not cause rupture of the meninges. The use of [14C]5-HIA...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(81)91187-2
更新日期：1981-03-23 00:00:00
