Abstract：
:The catalytic subunit of human DNA polymerase (pol) delta, p125, was expressed in recombinant baculovirus-infected insect cells, separated from a baculovirus-encoded DNA polymerase, and was purified to homogeneity by affinity trapping with a histidine-octapeptide at the C-terminus of p125 as the ligand. Purified p125 showed DNA polymerase activity resembling conventionally purified calf thymus pol delta. However, the two differed in four ways: 1) the specific activity of recombinant p125 was one quarter of the calf thymus pol delta; 2) the recombinant p125 was relatively resistant to aphidicolin; 3) the apparent Km for dTTP of the recombinant p125 was estimated at 33 microM, 15-fold the value for calf thymus pol delta; and 4) the recombinant p125 was not stimulated by recombinant PCNA, while activity of calf thymus pol delta increased 150-fold in response. Furthermore, PCNA did not stimulate either the p125 incubated with p50, a small subunit of pol delta, or co-expressed with p50 in insect cells. The full length recombinant p125 migrated slightly faster than pol delta from human cell lines, Jurkat or HeLa, upon SDS-polyacrylamide gel electrophoresis, suggesting a post-translational modification. The results indicate that in vivo assembly of the fully active complex of pol delta requires factors in addition to p125 and p50 subunits, and/or a post-translational modification of p125.
journal_name
Nagoya J Med Scijournal_title
Nagoya journal of medical scienceauthors
Suzuki S,Suzuki M,Yoshida Skeywords:
subject
Has Abstractpub_date
2000-11-01 00:00:00pages
99-113issue
3-4eissn
0027-7622issn
2186-3326journal_volume
63pub_type
杂志文章abstract：:Accessibility to health care services in Thailand is generally good, but a few areas in the country are difficult to reach. The purpose of this study was to investigate the accessibility of health care services in rural and remote areas of Thailand. It was conducted in 16 remote villages within a catchment area with a...
journal_title：Nagoya journal of medical science
pub_type： 杂志文章
doi：
更新日期：2013-08-01 00:00:00
abstract：:Temporary threshold shifts (TSSs) in vibrotactile perception produced by continuous vibration and repetitive shocks having one complete cycle of a 100 Hz sine wave and exponential decays. The repetition rate of the cycles was 5, 25, 50, or 100/s, while the root-mean-square (r.m.s.) acceleration measured over exposure ...
journal_title：Nagoya journal of medical science
pub_type： 杂志文章
doi：
更新日期：1994-05-01 00:00:00
abstract：:To evaluate the circulatory disturbances, sensory disturbances and damage to muscles and joints by chain saw vibration exposure, the process of the deterioration of the symptoms with chain saw operating time was studied. Subjects were classified into eight groups according to TOT (Total Operating Time): Group 0, 46 co...
journal_title：Nagoya journal of medical science
pub_type： 杂志文章
doi：
更新日期：1994-05-01 00:00:00
abstract：:Central diabetes insipidus (CDI), characterized by polyuria and polydipsia, is caused by deficiency of arginine vasopressin (AVP), an antidiuretic hormone which acts on V2 receptors in kidney to promote reabsorption of free water. CDI is classified into three subtypes; idiopathic, secondary and familial. A previous st...
journal_title：Nagoya journal of medical science
pub_type： 杂志文章,评审
doi：10.18999/nagjms.78.4.349
更新日期：2016-12-01 00:00:00
abstract：:We report a patient with a multicystic kidney treated successfully by continuous percutaneous drainage. This method may be an alternative to surgical resection of a multicystic kidney that has not involved after one year of follow-up. ...
journal_title：Nagoya journal of medical science
pub_type： 杂志文章
doi：
更新日期：1993-11-01 00:00:00
abstract：:Dialysis-induced hypotension, the sharp decrease in blood pressure occurring during hemodialysis, remains one of the most difficult problems associated with hemodialysis even today. However, there is yet no established theory to explain the mechanism triggering dialysis-induced hypotension. This review attempts to off...
journal_title：Nagoya journal of medical science
pub_type： 杂志文章,评审
doi：
更新日期：1992-03-01 00:00:00
abstract：:We investigated the differences in Japanese and United States medical and legal professional opinions on ethical support for clinical ethical issues using the refusal of blood transfusions on the grounds of religious principles as an example of a clinical ethical issue. In ethical support systems for medical instituti...
journal_title：Nagoya journal of medical science
pub_type： 杂志文章
doi：10.18999/nagjms.82.2.193
更新日期：2020-05-01 00:00:00
abstract：:Little is known about the carbohydrate structure of the platelet von Willebrand factor (vWf). We have analyzed N-linked oligosaccharides from both plasma and platelet vWf by Fluorophore-Assisted-Carbohydrate Electrophoresis (FACE) and isoelectric focusing before and after digestion of the proteins with neuraminidase. ...
journal_title：Nagoya journal of medical science
pub_type： 杂志文章
doi：
更新日期：2000-05-01 00:00:00
abstract：:Prostate specific antigen (PSA) testing plays a major role in prostate cancer screening; however, the low positive predictive value of PSA testing leads to many unnecessary biopsies. Genetic background is one of factors that could cause it. That's why an association between genetic background and PSA levels should be ...
journal_title：Nagoya journal of medical science
pub_type： 杂志文章,多中心研究
doi：
更新日期：2013-02-01 00:00:00
abstract：:Over the last two decades, many attempts have been made to record electroretinograms (ERG) of human macula by several investigators. However, previous ERG responses were not informative enough, because each ERG component could not be evaluated independently. However, we have developed a new system for recording focal ...
journal_title：Nagoya journal of medical science
pub_type： 杂志文章,评审
doi：
更新日期：1998-10-01 00:00:00
abstract：:The Japan Multi-Institutional Collaborative Cohort Study (J-MICC Study) is a long-term cohort study to investigate the interactions among genotypes, lifestyles, and lifestyle-related diseases, especially cancer. This article reports the outline of the baseline survey of the Daiko Study, one site of the J-MICC Study. T...
journal_title：Nagoya journal of medical science
pub_type： 杂志文章
doi：
更新日期：2011-08-01 00:00:00
abstract：:Hyperperfusion syndrome occurs after treatment of a large or giant cerebral aneurysm. Recently, flow-diverter stent placement has emerged as an effective treatment method for a large cerebral aneurysm, but postoperative ipsilateral delayed intraparenchymal hemorrhage occurs in a minority of cases. The mechanism underl...
journal_title：Nagoya journal of medical science
pub_type： 杂志文章
doi：10.18999/nagjms.81.4.629
更新日期：2019-11-01 00:00:00
abstract：:Patients with intestinal ischemia associated with acute aortic dissection often require emergent bowel resection, which results in serious complications. We present a case of successful surgical management of extensive bowel necrosis caused by acute aortic dissection. A 42-year-old man underwent emergent subtotal rese...
journal_title：Nagoya journal of medical science
pub_type：
doi：10.18999/nagjms.81.4.711
更新日期：2019-11-01 00:00:00
abstract：:In the present study, we developed quantitative index for evaluating regional liver function using 99mTc diethylenetriamine-pentaacetic acid galactosyl human serum albumin (GSA). A three head SPECT equipped with a low energy high resolution parallel hole collimator was used. Used pre-filter was Butterworth and axial i...
journal_title：Nagoya journal of medical science
pub_type： 杂志文章
doi：
更新日期：2003-05-01 00:00:00
abstract：:The purpose of this matched case-control study was to investigate the social correlates of primary infertility among females aged 35 years or less. The study was conducted in the Clinics of Samarkand Medical Institute, Uzbekistan, among 120 infertile and 120 healthy women matched by age, residential area, and occupati...
journal_title：Nagoya journal of medical science
pub_type： 杂志文章
doi：
更新日期：2012-08-01 00:00:00
abstract：:A 67-year-old man underwent posterior cervical decompression surgery for ossification of the posterior longitudinal ligament (OPLL) with fixation using cervical pedicle screws (CPSs) guided by intraoperative 3D image-based navigation. Intraoperatively, while creating the screw hole using the navigation probe, the virt...
journal_title：Nagoya journal of medical science
pub_type：
doi：10.18999/nagjms.82.4.799
更新日期：2020-11-01 00:00:00
abstract：:As molecular biology has developed, several new diagnostic techniques have found application in the clinical setting. The use of the polymerase chain reaction (PCR) assay to study the molecular biology of microbial organisms has led to unparalleled advances, largely due to the rapidity with which results can be obtain...
journal_title：Nagoya journal of medical science
pub_type： 杂志文章,评审
doi：
更新日期：1999-11-01 00:00:00
abstract：:Being homebound has been reported to be associated with a number of conditions. In the current study, the incidence of homebound individuals was surveyed in an urban city area in Japan. The city office randomly enrolled 5,000 residents of Nagoya City aged 65 and over. A questionnaire was sent to their principal caregi...
journal_title：Nagoya journal of medical science
pub_type： 杂志文章
doi：
更新日期：2015-08-01 00:00:00
abstract：:Autologous nerve transplantation has been the gold standard in the treatment of facial nerve injury, however it has not been achieved satisfactory result and needs donor sacrifice. A polyglycolic acid collagen conduit (Nerbridge, Toyobo Co., Japan) has the potential to compare to or exceed autologous nerve grafts in p...
journal_title：Nagoya journal of medical science
pub_type：
doi：10.18999/nagjms.82.1.123
更新日期：2020-02-01 00:00:00
abstract：:The influence of total ischemia on the formation of products of anaerobic metabolism was investigated in canine hearts in vitro. Contents of organic acids were quantitatively analyzed by gas chromatography-mass spectrometry, and high energy phosphate compounds were determined using the luciferin-luciferase enzyme syst...
journal_title：Nagoya journal of medical science
pub_type： 杂志文章,评审
doi：
更新日期：1994-03-01 00:00:00
abstract：:A study was conducted to clarify the prognostic factors related to recurrence of craniopharyngioma and to improve the quality of life of patients by the treatment with intentional partial removal and gamma knife radiosurgery. One hundred cases of craniopharyngioma have been treated at Komaki City Hospital since 1991. ...
journal_title：Nagoya journal of medical science
pub_type： 杂志文章
doi：
更新日期：2012-02-01 00:00:00
abstract：:Surgical intervention for the treatment of acetabular fracture non-union is often challenging. Here, we present a case of acetabular fracture non-union with pelvic discontinuity in a patient who underwent cemented total hip arthroplasty (THA) after intra- and extra-articular plate fixation. The case was a 70-year-old ...
journal_title：Nagoya journal of medical science
pub_type： 杂志文章
doi：10.18999/nagjms.81.1.113
更新日期：2019-02-01 00:00:00
abstract：:In endovascular coil embolization for intracranial aneurysms, as coils are filled in the aneurysm and the stage of procedure is advanced, the force to push forward the coil delivery wire (insertion force) increases. However, the coil insertion force that interventionist's felt at his fingertips does not directly refle...
journal_title：Nagoya journal of medical science
pub_type： 杂志文章
doi：10.18999/nagjms.81.2.217
更新日期：2019-05-01 00:00:00
abstract：:Surgeons focus on the period of absence from work during the initial treatment of breast cancer. The aim of this study was to determine surgeons' perceptions and awareness regarding the necessary period of absence from work during breast cancer treatment. We created a questionnaire for all surgeons involved in breast ...
journal_title：Nagoya journal of medical science
pub_type： 杂志文章
doi：
更新日期：2014-08-01 00:00:00
abstract：:A total of 483 hips treated by primary total hip arthroplasty (THA) were investigated to evaluate the surgical skill of the performing surgeon. Surgical trainees operated on 259 hips and instructors on 224 hips. The average age of the patients at the time of THA in the trainee and instructor group was 61.9 and 60.8 ye...
journal_title：Nagoya journal of medical science
pub_type： 杂志文章
doi：
更新日期：2015-02-01 00:00:00
abstract：:A case treated with onlay bone grafting and simultaneous osseointegrated implant insertion is reported. The patient had an oronasal fistula in the center of the premaxillary region due to failed repair of a bilateral cleft palate. Four fixtures were inserted with onlay bone grafting of the maxillary alveolar ridge. Tw...
journal_title：Nagoya journal of medical science
pub_type： 杂志文章
doi：
更新日期：1993-03-01 00:00:00
abstract：:Aging is accelerated by metabolic and cardiovascular diseases, and the risk of these diseases increases with age. Obesity is an important risk factor for many age-related diseases and is linked to reduced telomere length in white blood cells. We investigated whether cardiac senescence might be enhanced in DahlS.Z-Lepr...
journal_title：Nagoya journal of medical science
pub_type： 杂志文章
doi：
更新日期：2014-02-01 00:00:00
abstract：:Mediastinal embryonal carcinoma is rare, and the life prognosis of this disease is assumed to be relatively short. We encountered a case of mediastinal embryonal carcinoma for which we could perform radical surgical resection. The patient was male, aged 16 years, and acutely aware of back pain. Because the pain increa...
journal_title：Nagoya journal of medical science
pub_type： 杂志文章
doi：
更新日期：2014-02-01 00:00:00
abstract：:Anastomotic leakage after esophagectomy is associated with prolonged hospitalization and increased medical cost. Additionally, it sometimes leads to a fatal condition and impaired postoperative quality of life. During the process of wound healing, β-hydroxy-β-methylbutyrate (HMB) is important for collagen biosynthesis...
journal_title：Nagoya journal of medical science
pub_type： 杂志文章
doi：10.18999/nagjms.82.1.33
更新日期：2020-02-01 00:00:00
abstract：:Thoracoscopic lobectomy has recently become a widely used surgical treatment for congenital cystic lung disease, but significant issues can arise in some cases, such as a limited working space in neonates, a limited view in cases involving large cystic lesions. We reviewed the treatment outcomes of neonates that under...
journal_title：Nagoya journal of medical science
pub_type： 杂志文章
doi：10.18999/nagjms.78.4.447
更新日期：2016-12-01 00:00:00
