Abstract:
:N-type calcium channels both generate the initial calcium signal to trigger neurotransmitter release and also interact with synaptic release proteins at many mammalian central nervous system synapses. Two isoforms of the alpha 1B N-type channel from rat brain (alpha 1B-I and alpha 1B-II) were found to differ in four regions: (1) a glutamate (Glu) to glycine (Gly) substitution in domain I S3; (2) a Gly to Glu substitution in the domain I-II linker; (3) the insertion or deletion of an alanine (Ala) in the domain I-II linker; and (4) the presence or absence of serine/phenylalanine/methionine/glycine (SFMG) in the linker between domain III S3-S4. Comparison of the electrophysiological properties of the alpha 1B-I and alpha 1B-II N-type channels shows that they exhibit distinct kinetics as well as altered current-voltage relations. Utilizing chimeric alpha 1B-I and alpha 1B-II cDNAs, we show that: (1) the Glu 177 to Gly substitution in domain I S3 increases the rate of activation by approximately 15-fold; (2) the presence or absence of Ala 415 in the domain I-II linker alters current-voltage relations by approximately 10 mV but does not affect channel kinetics; (3) the substitution of Gly 387 to Glu in the domain I-II linker also has no effect on kinetics; and (4) the presence or absence of SFMG (1236-1239) in domain III S3-S4 did not significantly affect channel current-voltage relations, kinetics, or steady state inactivation. We conclude that molecularly distinct alpha 1B isoforms are expressed in rat brain and may account for some of the functional diversity of N-type currents in native cells.
journal_name
Ann N Y Acad Scijournal_title
Annals of the New York Academy of Sciencesauthors
Stea A,Dubel SJ,Snutch TPdoi
10.1111/j.1749-6632.1999.tb11282.xsubject
Has Abstractpub_date
1999-04-30 00:00:00pages
118-30eissn
0077-8923issn
1749-6632journal_volume
868pub_type
杂志文章abstract::Coccidioides causes coccidioidomycosis in the southwestern United States. Its clinical manifestations range from the primary asymptomatic to progressive pulmonary and extrapulmonary disease. Because of endemicity, frequent relapse, and virulent nature of Coccidioides, there is an urgent need for the development of eff...
journal_title:Annals of the New York Academy of Sciences
pub_type: 杂志文章,评审
doi:10.1196/annals.1406.013
更新日期:2007-09-01 00:00:00
abstract::The presence of R. prowazekii in lice using R. prowazekii-specific qPCR cassettes showed the changes of the rickettsial burden in the lice post-infection. ...
journal_title:Annals of the New York Academy of Sciences
pub_type: 杂志文章
doi:10.1196/annals.1374.036
更新日期:2006-10-01 00:00:00
abstract::We demonstrate that task relevance dissociates between visual awareness and knowledge activation to create a state of seeing without knowing-visual awareness of familiar stimuli without recognizing them. We rely on the fact that in order to experience a Kanizsa illusion, participants must be aware of its inducers. Whi...
journal_title:Annals of the New York Academy of Sciences
pub_type: 杂志文章,随机对照试验
doi:10.1111/nyas.12673
更新日期:2015-03-01 00:00:00
abstract::Pain is a subjective symptom whose prevalence can be grossly underestimated. The high proportion of adults with thalassemia who experience chronic pain is evident from recent surveys. However, pain has not received enough attention in the overall management of thalassemia. The association of pain with the type and tre...
journal_title:Annals of the New York Academy of Sciences
pub_type: 杂志文章,评审
doi:10.1111/nyas.13061
更新日期:2016-03-01 00:00:00
abstract::Lesion, stimulation, and pharmacological studies point to separate hypothalamic control of pulsatile FSH and LH secretion. LH release is controlled by a region extending from the preoptic area to the anterior and mid-median eminence, whereas FSH release is controlled by a region extending from the dorsal anterior hypo...
journal_title:Annals of the New York Academy of Sciences
pub_type: 杂志文章,评审
doi:10.1111/j.1749-6632.1993.tb43853.x
更新日期:1993-05-28 00:00:00
abstract::In adult Lurcher mice virtually all cerebellar Purkinje cells have degenerated as a direct consequence of mutant gene action, providing a natural model for studying the effect of cerebellar cortical lesions on the generation of compensatory eye movements. Lurcher mice possess both optokinetic (OKR) and vestibular (VOR...
journal_title:Annals of the New York Academy of Sciences
pub_type: 杂志文章
doi:10.1111/j.1749-6632.2002.tb07584.x
更新日期:2002-12-01 00:00:00
abstract::The first evidence for the role of the protein elongation factor eEF1A2 in tumorigenesis was reported by Anand and colleagues who demonstrated that eEF1A2 is overexpressed in about 30% of ovarian tumors and some established ovarian cancer cells. This abnormal expression correlates with a poor prognosis. Since this dis...
journal_title:Annals of the New York Academy of Sciences
pub_type: 杂志文章,评审
doi:10.1111/j.1749-6632.2009.04909.x
更新日期:2009-08-01 00:00:00
abstract::Clostridium difficile infection, also known as C. difficile-associated diarrhea (CDAD), is the most common cause of nosocomial diarrhea, typically initiated by the use of broad-spectrum antibiotics that disrupt gut flora, thereby allowing C. difficile to proliferate. It is an increasing cause of morbidity and mortalit...
journal_title:Annals of the New York Academy of Sciences
pub_type: 杂志文章,评审
doi:10.1111/nyas.12135
更新日期:2013-07-01 00:00:00
abstract::The aim of this study was to examine changes in muscle cell injury and antioxidant capacity of weightlifters following a 1-week intensive resistance-training regimen. Thirty-six female subjects participated in this study, and their ages ranged from 18 to 25 years. The sample group included 19 elite weightlifters with ...
journal_title:Annals of the New York Academy of Sciences
pub_type: 杂志文章
doi:10.1196/annals.1338.029
更新日期:2005-05-01 00:00:00
abstract::Altered tissue-specific processing or production of the amyloid precursor protein (APP) is thought to be central to amyloid deposition in cerebrovascular and the neocortical tissues in Alzheimer's disease (AD). We demonstrate that A beta peptide(s) is readily detectable and increased in cerebral vessels, meninges and ...
journal_title:Annals of the New York Academy of Sciences
pub_type: 杂志文章
doi:10.1111/j.1749-6632.1996.tb34434.x
更新日期:1996-01-17 00:00:00
abstract::The physiological status of presynaptic receptors, regulating sympathetic neurotransmitter release, remains subject to debate. Nevertheless, pharmacological techniques have shown presynaptic alpha-adrenoceptors, mediating a negative feedback inhibition of neuronal noradrenaline (NA) release, and presynaptic beta-adren...
journal_title:Annals of the New York Academy of Sciences
pub_type: 杂志文章,评审
doi:10.1111/j.1749-6632.1990.tb32007.x
更新日期:1990-01-01 00:00:00
abstract::The Balb/c mice were infected with Coxiella burnetii and samples of blood and major organs from the infected mice were collected on days 1 to 14 after infection. The DNAs extracted from the samples were detected by a developed real-time quantitative PCR specific for C. burnetii and high loads of C. burnetii were found...
journal_title:Annals of the New York Academy of Sciences
pub_type: 杂志文章
doi:10.1196/annals.1355.027
更新日期:2005-12-01 00:00:00
abstract::Internuclear ophthalmoparesis (INO) is the most common saccadic eye movement disorder observed in patients with multiple sclerosis (MS). It is characterized by slowing of the adducting eye during horizontal saccades, and most commonly results from a demyelinating lesion in the medial longitudinal fasciculus (MLF) with...
journal_title:Annals of the New York Academy of Sciences
pub_type: 杂志文章
doi:10.1111/j.1749-6632.2011.06125.x
更新日期:2011-09-01 00:00:00
abstract::Nicergoline (NE)--a cerebral vasodilator with nicotinic acid esterified in its molecule--and Antagonic-Stress (AS) composition--a neurometabolic nootropic, also containing nicotinic acid but with fast and prolonged release--were evaluated in senile dementia of Alzheimer's type (SDAT), mild to moderate intensity (DSM-I...
journal_title:Annals of the New York Academy of Sciences
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1111/j.1749-6632.1994.tb12102.x
更新日期:1994-06-30 00:00:00
abstract::In summary, the exon/intron structure of the chicken type II collagen gene is identical with that of the chicken alpha 2(I) collagen gene and differs at only one known position from the human and mouse alpha 1(I) genes. However, the chicken type II gene is different from the chicken alpha 2(I) gene in that it is consi...
journal_title:Annals of the New York Academy of Sciences
pub_type: 杂志文章
doi:10.1111/j.1749-6632.1985.tb51161.x
更新日期:1985-01-01 00:00:00
abstract::1 alpha,25-Dihydroxyvitamin D3, the active form of vitamin D3, induced maturation of circulating monocytes to form macrophage-epithelioid cells and multinucleated giant cells in vitro. Calcitriol not only promoted the differentiation of monocytes, as shown by the marked morphological changes and enhanced secretion of ...
journal_title:Annals of the New York Academy of Sciences
pub_type: 杂志文章
doi:10.1111/j.1749-6632.1986.tb18497.x
更新日期:1986-01-01 00:00:00
abstract::The screwworm fly, Cochliomyia hominivorax (Coquerel), is a parasite that attacks all warm-blooded animals including humans. This parasite has caused significant losses to the livestock industries of the Americas. Since the screwworm eradication program was initiated in the Southeastern United States in 1957, the erad...
journal_title:Annals of the New York Academy of Sciences
pub_type: 杂志文章
doi:10.1111/j.1749-6632.2000.tb05289.x
更新日期:2000-01-01 00:00:00
abstract::Emerging infectious diseases are of international concern because of the potential for, and impact of, pandemics; however, they are difficult to predict. To identify the drivers of disease emergence, we analyzed infectious disease threat events (IDTEs) detected through epidemic intelligence collected at the European C...
journal_title:Annals of the New York Academy of Sciences
pub_type: 杂志文章,评审
doi:10.1111/nyas.13132
更新日期:2016-10-01 00:00:00
abstract::The data presented above suggest that one possible clinical use of TGF-beta would be to protect the bone marrow from the effects of myelosuppressive chemotherapeutic drugs by preventing entry or removing primitive stem cells from the cell cycle. It may also have the additional benefit of reducing the drug-induced neut...
journal_title:Annals of the New York Academy of Sciences
pub_type: 杂志文章,评审
doi:10.1111/j.1749-6632.1991.tb17220.x
更新日期:1991-01-01 00:00:00
abstract:UNLABELLED:This article suggests eight high-level concepts that can promote convergence by identifying analogies across fields of science and engineering. CONSERVATION: Many properties are conserved, through symmetries, parity laws, and feedback-regulated stabilities in complex adaptive systems. INDECISION: Inconsisten...
journal_title:Annals of the New York Academy of Sciences
pub_type: 杂志文章,评审
doi:10.1196/annals.1382.003
更新日期:2006-12-01 00:00:00
abstract::We review the effects of two transcription factor decoy oligonucleotides on apoptosis of human osteoclasts (OCs). The first decoy molecule was designed to inhibit nuclear factor kappa-B (NF-kappaB) binding to target sequence, the second to increase estrogen receptor (ER) alpha expression. We found that both decoy mole...
journal_title:Annals of the New York Academy of Sciences
pub_type: 杂志文章,评审
doi:10.1196/annals.1378.092
更新日期:2006-12-01 00:00:00
abstract::The receptive field of a direction-selective unit of any type consists of an excitatory center 3 degrees -5 degrees in size, a near inhibitory surround and a far surround upon which illumination exerts an influence for both spontaneous activity and specific cell response. ...
journal_title:Annals of the New York Academy of Sciences
pub_type: 杂志文章
doi:10.1196/annals.1342.057
更新日期:2005-06-01 00:00:00
abstract::Trichostatin A (TSA), an inhibitor of the eukaryotic cell cycle and an inducer of morphological reversion of transformed cells, inhibits histone deacetylase (HDAC) at nanomolar concentrations. Recently, trapoxin, oxamflatin, and FR901228, antitumor agents structurally unrelated to TSA, were found to be potent HDAC inh...
journal_title:Annals of the New York Academy of Sciences
pub_type: 杂志文章,评审
doi:10.1111/j.1749-6632.1999.tb09397.x
更新日期:1999-01-01 00:00:00
abstract::A hallmark of success for early career biomedical researchers is the acquisition of research funding. There are marked disparities among principal investigators who submit grants and the likelihood of receiving national funding. The National Research Mentoring Network was funded by the National Institutes of Health to...
journal_title:Annals of the New York Academy of Sciences
pub_type: 杂志文章
doi:10.1111/nyas.13995
更新日期:2019-06-01 00:00:00
abstract::Gene therapy as an immunomodulatory approach has the potential to treat various inherited and acquired immune-based human diseases. However, its clinical application has several challenges, varying from the efficiency of gene transfer, control of gene expression, cell and tissue targeting, and safety concerns associat...
journal_title:Annals of the New York Academy of Sciences
pub_type: 杂志文章
doi:10.1196/annals.1352.036
更新日期:2005-11-01 00:00:00
abstract::In the inflammatory synovium production of collagenase is probably responsible for the degradation of collagen in the extracellular matrix and distortion of the architecture and function of the joints. Major collagenase-producing cells are mesenchymal cells such as fibroblasts and chondrocytes, which synthesize and se...
journal_title:Annals of the New York Academy of Sciences
pub_type: 杂志文章,评审
doi:10.1111/j.1749-6632.1990.tb17943.x
更新日期:1990-01-01 00:00:00
abstract::Over seventy percent of unexpected infant deaths are registered as SIDS. Over 85,000 infants have been screened at birth and one month of age for risk of unexpected death using the Sheffield Score system. Scores range from below 400 to over 800 points. Infants with scores over 800 are at more than 16 times greater ris...
journal_title:Annals of the New York Academy of Sciences
pub_type: 杂志文章
doi:10.1111/j.1749-6632.1988.tb37237.x
更新日期:1988-01-01 00:00:00
abstract::While dopamine (DA) receptors mediate acute effects of amphetamine and cocaine, chronic drug administration produces many glutamate-dependent adaptations, including LTP in reward-related neuronal circuits. An important question presents itself: How do DA receptors influence glutamate-dependent synaptic plasticity? Alt...
journal_title:Annals of the New York Academy of Sciences
pub_type: 杂志文章,评审
doi:10.1196/annals.1300.015
更新日期:2003-11-01 00:00:00
abstract::The entry of resting T cells into the G1 phase of the cell cycle after stimulation by mitogens is controlled by a series of biochemical events that are independent of growth factors. These events follow the initial signals stimulated through the engagement of the T-cell receptor and include activation of the cyclin-de...
journal_title:Annals of the New York Academy of Sciences
pub_type: 杂志文章
doi:10.1111/j.1749-6632.1995.tb26657.x
更新日期:1995-09-07 00:00:00
abstract::Human epidemiologic studies provide convincing evidence that dietary patterns practiced during adulthood are important contributors to age-related cognitive decline and dementia risk. Diets high in fat, especially trans and saturated fats, adversely affect cognition, while those high in fruits, vegetables, cereals, an...
journal_title:Annals of the New York Academy of Sciences
pub_type: 杂志文章,评审
doi:10.1196/annals.1396.028
更新日期:2007-10-01 00:00:00