Abstract:
:Both humoral and cellular immune responses are inducible by inoculation of naked plasmid DNA encoding a polypeptide antigen. This new vaccination protocol, known as genetic immunization, has been used to initiate protective immunity against a variety of infectious pathogens and tumors in experimental animals. Dendritic cells (DC) are thought to play at least three distinct roles in genetic immunization: (1) MHC class II-restricted presentation of antigens secreted by neighboring, transfected cells, (2) MHC class I-restricted "cross" presentation of antigens released by neighboring, transfected cells, and (3) direct presentation of antigens by transfected DC themselves. Several new technologies have been developed recently in an attempt to improve the overall efficacy of genetic vaccination, as well as to regulate the type and class of resulting immune responses. These technologies include modification of plasmid DNA, co-delivery of genes encoding immunoregulatory molecules, and DC targeting. We will overview some of these new technologies in genetic immunization.
journal_name
J Leukoc Bioljournal_title
Journal of leukocyte biologyauthors
Takashima A,Morita Adoi
10.1002/jlb.66.2.350subject
Has Abstractpub_date
1999-08-01 00:00:00pages
350-6issue
2eissn
0741-5400issn
1938-3673journal_volume
66pub_type
杂志文章,评审abstract::Dendritic cell (DC) and DC-derived exosomes (EXO) have been used extensively for tumor vaccination. However, its therapeutic efficiency is limited to only production of prophylactic immunity against tumors. T cells can uptake DC-released EXO. However, the functional effect of transferred exosomal molecules on T cells ...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1189/jlb.0407249
更新日期:2007-10-01 00:00:00
abstract::The Fanconi anemia proteins participate in a canonical pathway that repairs cross-linking agent-induced DNA damage. Cells with inactivated Fanconi anemia genes are universally hypersensitive to such agents. Fanconi anemia-deficient hematopoietic stem cells are also hypersensitive to inflammatory cytokines, and, as imp...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1189/jlb.3A0515-201R
更新日期:2016-03-01 00:00:00
abstract::Tissue macrophages produce several proteins of the complement system. The mechanisms that regulate this process are poorly understood. The established ability of certain prostaglandins to influence macrophage secretory activity suggests that these lipid mediators may also modulate complement production (CP). Using the...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1002/jlb.37.5.651
更新日期:1985-05-01 00:00:00
abstract::Eosinophils were isolated from peritoneal lavages of repeated horse serum-injected guinea pigs or rhesus monkeys and from peripheral blood of normal human donors. Eicosanoid metabolism and chemotaxis by these cells were studied by in vitro techniques. Upon calcium ionophore stimulation guinea pig eosinophils released ...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1002/jlb.50.2.140
更新日期:1991-08-01 00:00:00
abstract::Neutrophils are quickly recruited to tissues in response to proinflammatory cues; however, little is known about tissue neutrophil phenotypes in health. We employ a multicolor flow cytometric approach to assess surface markers of activation on neutrophils from the bone marrow, blood, peritoneum, spleen, liver, fat, co...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1002/JLB.4AB1020-248RRR
更新日期:2020-11-04 00:00:00
abstract::Alum, aluminum-hydroxide-containing compounds, long used as adjuvants in human vaccinations, functions by ill-defined, immunostimulatory mechanisms. Antigen-free alum has been shown to act via a previously unidentified, splenic Gr1(+), IL-4-expressing myeloid cell population to stimulate early B cell priming. We demon...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1189/jlb.0607392
更新日期:2008-04-01 00:00:00
abstract::In vivo, dendritic cells (DC) form a network comprising different populations. In particular, Langerhans cells (LC) appear as a unique population of cells dependent on transforming growth factor beta(TGF-beta) for its development. In this study, we show that endogenous TGF-beta is required for the development of both ...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1002/jlb.66.5.781
更新日期:1999-11-01 00:00:00
abstract::Mast cells and MDSCs are increased by parasitic infection and tumor growth. We previously demonstrated that enhanced MDSC development in ADAM10 transgenic mice yielded resistance to Nb infection and that coculturing MDSCs and mast cells enhanced cytokine production. In the current work, we show that MDSC-mast cell coc...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1189/jlb.0913510
更新日期:2014-04-01 00:00:00
abstract::Polyclonal B cell activation is not a peculiar characteristic to a particular infection, as many viruses, bacteria, and parasites induce a strong polyclonal B cell response resulting in hyper-gamma-globulinemia. Here, we discuss the different roles proposed for polyclonal B cell activation, which can be crucial for ea...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章,评审
doi:10.1189/jlb.0407214
更新日期:2007-11-01 00:00:00
abstract::The human lysozyme (LZM) gene is highly methylated in LZM-nonexpressor immature myeloid and in nonmyeloid cells and unmethylated only in LZM-expressing cells. Extended methylation analyses of the CpG-poor 5' flanking region and of the exon 4 CpG island (both containing Alu elements) of the LZM gene were now performed....
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1189/jlb.0106005
更新日期:2006-12-01 00:00:00
abstract::Dendritic cells (DCs) are specialized APCs present in all tissues, including tumors. They play a major role in orchestrating immune responses and were shown to occur in various functional states in tumors. In this respect, immunogenic tumor-associated DCs (TADCs) are required to initiate and sustain T cell-dependent a...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章,评审
doi:10.1189/jlb.4MR1116-466R
更新日期:2017-08-01 00:00:00
abstract::Attempts to analyze bone marrow-derived macrophages (BMDM) by flow cytometry have been prohibited because of their high autofluorescence. Using an autofluorescence reduction method of Steinkamp and Stewart to reduce the autofluorescence of BMDM, we were able to examine several macrophage populations for their expressi...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1002/jlb.43.6.557
更新日期:1988-06-01 00:00:00
abstract::The last 10-15 years have witnessed a revolution in treating multiple myeloma, an incurable cancer of Ab-producing plasma cells. Advances in myeloma therapy were ushered in by novel agents that remodel the myeloma immune microenvironment. The first generation of novel agents included immunomodulatory drugs (thalidomid...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章,评审
doi:10.1189/jlb.3MR1116-468R
更新日期:2017-08-01 00:00:00
abstract::Asthma is a chronic inflammatory disease of the airways that afflicts over 30 million individuals in the United States and over 300 million individuals worldwide. The inflammatory response in the airways is often characterized by the analysis of sputum, which contains multiple types of cells including neutrophils, mac...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1002/JLB.5MA0720-770RR
更新日期:2020-11-01 00:00:00
abstract::The specific binding of human polymorphonuclear leukocytes (PMN) to antibody-coated target cells was characterized by flow cytometry. PMN were labeled with phycoerythrin-E (PE) via a granulocyte-specific monoclonal antibody (leu-M1) and mixed with fluorescein isothiocyanate-labeled K562 tumor cells sensitized with rab...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1002/jlb.46.5.467
更新日期:1989-11-01 00:00:00
abstract::The role of JNK in neutrophil chemotaxis and killing of microbial pathogens remains unclear. Using a recently described cell-permeable peptide inhibitor of the JNK pathway, based on the JBD of JIP-1, coupled to the protein transduction domain of HIV-TAT (TAT-JIP), in association with control peptides, we demonstrate t...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1189/jlb.0609399
更新日期:2010-05-01 00:00:00
abstract::THP-1 cells are a monocyte-like cell line derived from a patient with acute monocytic leukemia and unlike other leukemic cell lines has a normal diploid karyotype. We have characterized Fc gamma R expression on this cell line by flow cytometry, radiolabeled IgG1 and monoclonal antibody (mAb) binding assays, and bioche...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1002/jlb.49.6.556
更新日期:1991-06-01 00:00:00
abstract::The objective of this study was to find the role of LncRNA SNHG12 in the regulation of hypertensive vascular endothelial injury. LncRNA SNHG12 and miR-25-3p expression were detected by quantitative RT-PCR. Protein levels of Sirtuin 6 (SIRT6), endothelial cell (EC) senescence markers p16 and p21, and EC marker CD31 wer...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1002/JLB.1A0820-501R
更新日期:2021-01-19 00:00:00
abstract::Mucosa represents the main site of pathogen/cell interactions. The two main types of cells forming the epithelial structure [epithelial cells and Langerhans cells (LC)] coordinate the first defense responses to avoid infection. To evaluate the involvement of epithelial cells in the early steps leading to a specific ad...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1189/jlb.0305147
更新日期:2005-07-01 00:00:00
abstract::Mer signaling participates in a novel inhibitory pathway in TLR activation. The purpose of the present study was to examine the role of Mer signaling in the down-regulation of TLR4 activation-driven immune responses in mice, i.t.-treated with LPS, using the specific Mer-blocking antibody. At 4 h and 24 h after LPS tre...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1189/jlb.0611289
更新日期:2012-06-01 00:00:00
abstract::To assess the effect of hypoxemia on the responses of polymorphonuclear neutrophils (PMN) during an inflammatory response, rats were maintained in a low F1O2 atmosphere (9% O2) or room air for 12 h before intrathoracic injection of carrageenin or intradermal injections of agonists. After 4 h, hypoxemic rats had 50% mo...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1002/jlb.67.6.785
更新日期:2000-06-01 00:00:00
abstract::Several alterations in the expression of immune-related transcripts were identified recently in the degenerating retina of the retinoschisin knockout (Rs1h(-/Y)) mouse, including the strong expression of the adaptor protein Dap12. As Dap12 is found in leukocytes, we hypothesized that its disease-related expression may...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1189/jlb.0707447
更新日期:2007-12-01 00:00:00
abstract::We investigated the ability of the sPLA(2), known as MT-III, isolated from the viperid snake Bothrops asper, to induce LB formation in macrophages and the major cellular signaling pathways involved in this process. The effects of MT-III on ADRP localization and expression and macrophage ultrastructure were assessed. O...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1189/jlb.0510263
更新日期:2011-07-01 00:00:00
abstract::That monocytes can differentiate into macrophages or dendritic cells (DCs) makes them an essential link between innate and adaptive immunity. However, little is known about how interactions with pathogens or T cells influence monocyte engagement toward DCs. We approached this point in cultures where granulocyte macrop...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:
更新日期:2002-12-01 00:00:00
abstract::We have shown previously that homotypic interaction of resting memory CD4 T cells with activated T cells induces the production of cytokines with immunoregulatory potential (IL-10, IL-4) from the former. Here, we analyzed the effector functions of these T cells stimulated by homotypic T cell interaction. T cells induc...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1189/jlb.0310122
更新日期:2010-11-01 00:00:00
abstract::The Hedgehog (Hh) family of signaling molecules functions in the development of numerous tissues during embryogenesis and has also been involved in adult self-renewing tissues. Recent results have demonstrated that the different components of the Hh signaling pathway are expressed in the human thymus. In this study, w...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1189/jlb.1107792
更新日期:2008-06-01 00:00:00
abstract::Macrophage-like tumor cells can be obtained in large quantities as rather homogeneous populations, making these cells useful for chemotaxis assays. Therefore, macrophage-like cells J774A, WEHI-3, P388D1, IC-21, and NCTC 1469, all of murine origin, and U937 of human origin, were tested for chemotactic activity to a num...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1002/jlb.38.6.747
更新日期:1985-12-01 00:00:00
abstract::Tumor necrosis factor alpha (TNF-alpha) has been shown to induce the production of interstitial collagenase by fibroblasts and chondrocytes. We investigated the role of TNF-alpha in collagenase gene expression by U937 monocyte/macrophage cells. Transcription of the TNF-alpha gene was observed after 0.5 h of phorbol my...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
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更新日期:1996-01-01 00:00:00
abstract::Interferons (IFN) and lipopolysaccharide (LPS) cause multiple changes in isoprenoid-modified proteins in murine macrophages, the most dramatic being the expression of a prenyl protein of 65 kDa. The guanylate binding proteins (GBPs) are IFN-inducible GTP-binding proteins of approximately 65 kDa that possess a CaaX mot...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1002/jlb.60.3.423
更新日期:1996-09-01 00:00:00
abstract::Glucocorticoids, such as dexamethasone (Dex), are used clinically in the treatment of various inflammatory diseases. Dex acts by inhibiting the expression of inflammatory mediators, such as tumor necrosis factor alpha (TNF-alpha) and monocyte chemoattractant protein-1 (MCP-1). It is surprising that Dex enhances bacter...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1189/jlb.0204068
更新日期:2004-07-01 00:00:00