Abstract:
:We have analyzed the distribution of type II collagen N- and C-propeptides in the cell layers and culture medium of bovine articular chondrocyte pellet cultures. Two splice variants of the type II collagen N-propeptide were detected by immunoblotting and immunoassay, using a new anti-peptide antibody, while the C-propeptide was detected using a monoclonal antibody. Type II collagen molecules containing the N-propeptide were detected weakly in cell layers, but not in tissue culture medium of chondrocyte pellet cultures, and both splice variants were observed. Free N-propeptide could not be detected in cell layers or medium. Type II procollagen molecules containing the C-propeptide were detected strongly in cell layers, but not in tissue culture medium, while the free C-propeptide was detected in both cell layers and medium. Since the N- and C-propeptides must be synthesized in a 1:1 molar ratio, we conclude that the N-propeptide is metabolized more quickly than the C-propeptide in this system. Our model can be used to study regulation of procollagen synthesis and propeptidase activity.
journal_name
J Cell Biochemjournal_title
Journal of cellular biochemistryauthors
Rebuck N,Croucher LJ,Hollander APdoi
10.1002/(sici)1097-4644(19991001)75:1<13::aid-jcb2subject
Has Abstractpub_date
1999-10-01 00:00:00pages
13-21issue
1eissn
0730-2312issn
1097-4644pii
10.1002/(SICI)1097-4644(19991001)75:1<13::AID-JCB2journal_volume
75pub_type
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