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The detection of viral genomes by polymerase chain reaction in the myocardium of pediatric patients with advanced HIV disease.

Abstract：

OBJECTIVES:The aim of this study was to investigate the frequency of viral nucleic acid detection in the myocardium of human immunodeficiency virus (HIV)-infected children to determine whether an association exists with the development of heart disease. BACKGROUND:As improved medical interventions increase the life expectancy of HIV-infected patients, increased incidences of myocarditis and dilated cardiomyopathy (DCM) are becoming more apparent, even in patients without clinical symptoms. METHODS:Myocardial samples were obtained from the postmortem hearts of 32 HIV-infected children and from 32 age-matched controls consisting of patients with structural congenital heart disease and no myocardial inflammation and no cardiac or systemic viral infection. The hearts were examined histologically and analyzed for the presence of viral sequences by polymerase chain reaction (PCR) or reverse transcription-PCR. RESULTS:Myocarditis was detected histologically in 11 of the 32 HIV-infected patients, and borderline myocarditis was diagnosed in another 13 cases. Infiltrates were confined to the epicardium in two additional hearts. Virus sequences were detected by PCR in 11 of these 26 cases (42.3%); adenovirus in 6, CMV in 3 and both adenovirus and CMV in 2. Two cases without infiltrates were also positive for adenovirus: one had congestive heart failure (CHF) and the other adenoviral pneumonia. No other viruses were detected by PCR, including HIV proviral DNA. All control samples were negative for all viruses tested. CONCLUSIONS:These data suggest that the presence of viral nucleic acid in the myocardium is common in HIV-infected children, and may relate to the development of myocarditis, DCM or CHF and may contribute to the rapid progression of HIV disease.

journal_name

J Am Coll Cardiol

journal_title

Journal of the American College of Cardiology

authors

Bowles NE,Kearney DL,Ni J,Perez-Atayde AR,Kline MW,Bricker JT,Ayres NA,Lipshultz SE,Shearer WT,Towbin JA

doi

10.1016/s0735-1097(99)00264-8

subject

Has Abstract

pub_date

1999-09-01 00:00:00

pages

857-65

issue

eissn

0735-1097

issn

1558-3597

pii

S0735-1097(99)00264-8

journal_volume

pub_type

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The detection of viral genomes by polymerase chain reaction in the myocardium of pediatric patients with advanced HIV disease.