Distinct roles for nitric oxide in resistant C57BL/6 and susceptible BALB/c mice to control Burkholderia pseudomallei infection.

Abstract:

BACKGROUND:Burkholderia pseudomallei is the causative agent of melioidosis, an emerging bacterial infectious disease in tropical and subtropical areas. We recently showed that NADPH oxidase but not nitric oxide (NO) contributes to resistance in innately resistant C57BL/6 mice in a B. pseudomallei respiratory infection model. However, the function of NO for resistance was shown to differ among distinct strains of mice and proved also to be stage dependent in various infection models. The present study therefore aimed to examine the role of NO in a systemic infection model of melioidosis and to test whether the function of NO differs among innately resistant C57BL/6 and susceptible BALB/c mice after B. pseudomallei infection. RESULTS:C57BL/6 iNOS-/- mice that were intravenously infected with B. pseudomallei survived several weeks, whereas most of the wild type animals succumbed during this period. The bacterial burden in liver and spleen was significantly higher in wild type animals compared to iNOS-/- mice 13 days after challenge. In contrast, BALB/c mice that were treated with amminoguanidine to inhibit NO expression in vivo showed significantly enhanced mortality rates and higher bacterial loads in liver and spleen compared to control animals. The bactericidal function of IFN-γ stimulated C57BL/6 iNOS-/- macrophages were not altered after B. pseudomallei infection, but BALB/c macrophages exhibited reduced killing activity against the pathogen when NO was inhibited. CONCLUSION:Our present data indicate a dual role of NO among resistant and susceptible mouse strains after B. pseudomallei infection. NO mediated mechanisms are an essential component to control the infection in susceptible BALB/c mice. In contrast, NO production in B. pseudomallei infected C57BL/6 mice rather harmed the host likely due to its detrimental effects.

journal_name

BMC Immunol

journal_title

BMC immunology

authors

Breitbach K,Wongprompitak P,Steinmetz I

doi

10.1186/1471-2172-12-20

subject

Has Abstract

pub_date

2011-03-16 00:00:00

pages

20

issn

1471-2172

pii

1471-2172-12-20

journal_volume

12

pub_type

杂志文章
  • Selection of reliable reference genes for the normalisation of gene expression levels following time course LPS stimulation of murine bone marrow derived macrophages.

    abstract:BACKGROUND:Macrophages are key players in the initiation, perpetuation and regulation of both innate and adaptive immune responses. They largely perform these roles through modulation of the expression of genes, especially those encoding cytokines. Murine bone marrow derived macrophages (BMDMs) are commonly used as a m...

    journal_title:BMC immunology

    pub_type: 杂志文章

    doi:10.1186/s12865-017-0223-y

    authors: Tanaka A,To J,O'Brien B,Donnelly S,Lund M

    更新日期:2017-10-03 00:00:00

  • The role of CXCR3 and its ligands expression in Brucellar spondylitis.

    abstract:AIM:Brucellar spondylitis (BS) is one of the most serious complications of brucellosis. CXCR3 is closely related to the severity of disease infection. This research aimed to study the degree of BS inflammatory damage through analyzing the expression levels of CXCR3 and its ligands (CXCL9 and CXCL10) in patients with BS...

    journal_title:BMC immunology

    pub_type: 杂志文章

    doi:10.1186/s12865-020-00390-9

    authors: Hu X,Shang X,Wang L,Fan J,Wang Y,Lv J,Nazierhan S,Wang H,Wang J,Ma X

    更新日期:2020-11-18 00:00:00

  • Immunome knowledge base (IKB): an integrated service for immunome research.

    abstract:BACKGROUND:Functioning of the immune system requires the coordinated expression and action of many genes and proteins. With the emergence of high-throughput technologies, a great amount of molecular data is available for the genes and proteins of the immune system. However, these data are scattered into several databas...

    journal_title:BMC immunology

    pub_type: 杂志文章

    doi:10.1186/1471-2172-10-3

    authors: Ortutay C,Vihinen M

    更新日期:2009-01-09 00:00:00

  • Inhibitory effects of the JAK inhibitor CP690,550 on human CD4(+) T lymphocyte cytokine production.

    abstract:BACKGROUND:The new JAK3 inhibitor, CP690,550, has shown efficacy in the treatment of rheumatoid arthritis. The present study was undertaken to assess the effects of CP690,550 on cytokine production and cellular signaling in human CD4(+) T cells. RESULTS:CD4(+) T cells produced IL-2, IL-4, IL-17, IL-22 and IFN-γ in fol...

    journal_title:BMC immunology

    pub_type: 杂志文章

    doi:10.1186/1471-2172-12-51

    authors: Migita K,Miyashita T,Izumi Y,Koga T,Komori A,Maeda Y,Jiuchi Y,Aiba Y,Yamasaki S,Kawakami A,Nakamura M,Ishibashi H

    更新日期:2011-08-31 00:00:00

  • Differential expression of CD300a/c on human TH1 and TH17 cells.

    abstract:BACKGROUND:Human memory CD4+ T cells can be either CD300a/c+ or CD300a/c- and subsequent analyses showed that CD4+ effector memory T (T(EM)) cells are mostly CD300a/c+, whereas CD4+ central memory T (T(CM)) cells have similar frequencies of CD300a/c+ and CD300a/c- cells. RESULTS:Extensive phenotypical and functional c...

    journal_title:BMC immunology

    pub_type: 杂志文章

    doi:10.1186/1471-2172-12-62

    authors: Simhadri VR,Mariano JL,Zhou Q,DeBell KE,Borrego F

    更新日期:2011-11-02 00:00:00

  • Differential utilization of NF-kappaB RELA and RELB in response to extracellular versus intracellular polyIC stimulation in HT1080 cells.

    abstract:BACKGROUND:Pattern recognition receptors (PRRs) for double-stranded RNA (dsRNA) are components of innate immunity that recognize the presence of viral infection and initiate efficient defense mechanisms. In addition to previously well-characterized signaling pathways that are mediated by PKR and TLR3, new intracellular...

    journal_title:BMC immunology

    pub_type: 杂志文章

    doi:10.1186/1471-2172-12-15

    authors: Yun JJ,Tsao MS,Der SD

    更新日期:2011-02-10 00:00:00

  • Interleukin-10 production by tumor infiltrating macrophages plays a role in Human Papillomavirus 16 tumor growth.

    abstract:BACKGROUND:Human Papillomavirus, HPV, is the main etiological factor for cervical cancer. Different studies show that in women infected with HPV there is a positive correlation between lesion grade and number of infiltrating macrophages, as well as with IL-10 higher expression. Using a HPV16 associated tumor model in m...

    journal_title:BMC immunology

    pub_type: 杂志文章

    doi:10.1186/1471-2172-11-27

    authors: Bolpetti A,Silva JS,Villa LL,Lepique AP

    更新日期:2010-06-07 00:00:00

  • Streptococcus pneumoniae stabilizes tumor necrosis factor alpha mRNA through a pathway dependent on p38 MAPK but independent of Toll-like receptors.

    abstract:BACKGROUND:Streptococcus pneumoniae is a human pathogenic bacteria and a major cause of severe invasive diseases, including pneumonia, bacteremia, and meningitis. Infections with S. pneumoniae evoke a strong inflammatory response, which plays a major role in the pathogenesis of pneumococcal disease. RESULTS:In this st...

    journal_title:BMC immunology

    pub_type: 杂志文章

    doi:10.1186/1471-2172-9-52

    authors: Mogensen TH,Berg RS,Ostergaard L,Paludan SR

    更新日期:2008-09-16 00:00:00

  • The DNA methyltransferase inhibitor, guadecitabine, targets tumor-induced myelopoiesis and recovers T cell activity to slow tumor growth in combination with adoptive immunotherapy in a mouse model of breast cancer.

    abstract:BACKGROUND:Myeloid derived suppressor cells (MDSCs) present a significant obstacle to cancer immunotherapy because they dampen anti-tumor cytotoxic T cell responses. Previous groups, including our own, have reported on the myelo-depletive effects of certain chemotherapy agents. We have shown previously that decitabine ...

    journal_title:BMC immunology

    pub_type: 杂志文章

    doi:10.1186/s12865-020-0337-5

    authors: Luker AJ,Graham LJ,Smith TM Jr,Camarena C,Zellner MP,Gilmer JS,Damle SR,Conrad DH,Bear HD,Martin RK

    更新日期:2020-02-27 00:00:00

  • The discriminative capacity of soluble Toll-like receptor (sTLR)2 and sTLR4 in inflammatory diseases.

    abstract:BACKGROUND:The extracellular domains of cytokine receptors are released during inflammation, but little is known about the shedding of Toll-like receptors (TLR) and whether they can be used as diagnostic biomarkers. METHODS:The release of sTLR2 and sTLR4 was studied in in-vitro stimulations, as well as in-vivo during ...

    journal_title:BMC immunology

    pub_type: 杂志文章

    doi:10.1186/s12865-014-0055-y

    authors: Ten Oever J,Kox M,van de Veerdonk FL,Mothapo KM,Slavcovici A,Jansen TL,Tweehuysen L,Giamarellos-Bourboulis EJ,Schneeberger PM,Wever PC,Stoffels M,Simon A,van der Meer JW,Johnson MD,Kullberg BJ,Pickkers P,Pachot A,Jooste

    更新日期:2014-11-19 00:00:00

  • Streptococcus sanguinis-induced cytokine and matrix metalloproteinase-1 release from platelets.

    abstract:BACKGROUND:Streptococcus sanguinis (S.sanguinis), a predominant bacterium in the human oral cavity, has been widely associated with the development of infective endocarditis. Platelets play both a haemostatic function and can influence both innate and adaptive immune responses. Previous studies have shown that S.sangui...

    journal_title:BMC immunology

    pub_type: 杂志文章

    doi:10.1186/1471-2172-15-15

    authors: Cognasse F,Hamzeh-Cognasse H,Chabert A,Jackson E,Arthaud CA,Garraud O,McNicol A

    更新日期:2014-04-22 00:00:00

  • Longitudinal immunosequencing in healthy people reveals persistent T cell receptors rich in highly public receptors.

    abstract:BACKGROUND:The adaptive immune system maintains a diversity of T cells capable of recognizing a broad array of antigens. Each T cell's specificity for antigens is determined by its T cell receptors (TCRs), which together across all T cells form a repertoire of millions of unique receptors in each individual. Although m...

    journal_title:BMC immunology

    pub_type: 杂志文章

    doi:10.1186/s12865-019-0300-5

    authors: Chu ND,Bi HS,Emerson RO,Sherwood AM,Birnbaum ME,Robins HS,Alm EJ

    更新日期:2019-06-21 00:00:00

  • Multi-layered Gag-specific immunodominant responses contribute to improved viral control in the CRF01_AE subtype of HIV-1-infected MSM subjects.

    abstract:BACKGROUND:The purpose of this study was to characterize specific cytotoxic T-cell (CTL) responses in men who have sex with men (MSM) subjects infected with the human immunodeficiency virus type 1 (HIV-1) CRF01_AE subtype during the first year of infection and impacts on viral control and evolution. RESULTS:Fifteen HI...

    journal_title:BMC immunology

    pub_type: 杂志文章

    doi:10.1186/s12865-016-0166-8

    authors: Jiang F,Han X,Zhang H,Zhao B,An M,Xu J,Chu Z,Dong T,Shang H

    更新日期:2016-08-30 00:00:00

  • Ten-color flow cytometry reveals distinct patterns of expression of CD124 and CD126 by developing thymocytes.

    abstract:BACKGROUND:We have developed a 12-parameter/10-color flow cytometric staining method for the simultaneous detection and characterization of 21 mouse thymocyte subpopulations that represent discreet stages of T cell development. To demonstrate the utility of this method, we assessed cytokine receptor expression on mouse...

    journal_title:BMC immunology

    pub_type: 杂志文章

    doi:10.1186/1471-2172-12-36

    authors: Tan C,Taylor AA,Coburn MZ,Marino JH,Van De Wiele CJ,Teague TK

    更新日期:2011-06-20 00:00:00

  • The innate interferon gamma response of BALB/c and C57BL/6 mice to in vitro Burkholderia pseudomallei infection.

    abstract:BACKGROUND:Burkholderia pseudomallei is the causative agent for melioidosis. For many bacterial infections, cytokine dysregulation is one of the contributing factors to the severe clinical outcomes in the susceptible hosts. The C57BL/6 and BALB/c mice have been established as a differential model of susceptibility in m...

    journal_title:BMC immunology

    pub_type: 杂志文章

    doi:10.1186/1471-2172-7-19

    authors: Koo GC,Gan YH

    更新日期:2006-08-18 00:00:00

  • Modulation of neutrophil function by the tripeptide feG.

    abstract:BACKGROUND:Neutrophils are critical in the defense against potentially harmful microorganisms, but their excessive and inappropriate activation can contribute significantly to tissue damage and a worsening pathology. Through the release of endocrine factors submandibular glands contribute to achieving a balance in neut...

    journal_title:BMC immunology

    pub_type: 杂志文章

    doi:10.1186/1471-2172-4-3

    authors: Mathison RD,Befus AD,Davison JS,Woodman RC

    更新日期:2003-03-04 00:00:00

  • Subsets of mononuclear phagocytes are enriched in the inflamed colons of patients with IBD.

    abstract:BACKGROUND:Myeloid cells, especially mononuclear phagocytes, which include monocytes, macrophages and dendritic cells (DC), play vital roles in innate immunity, and in the initiation and maintenance of adaptive immunity. While T cell-associated activation pathways and cytokines have been identified and evaluated in inf...

    journal_title:BMC immunology

    pub_type: 杂志文章

    doi:10.1186/s12865-019-0322-z

    authors: Liu H,Dasgupta S,Fu Y,Bailey B,Roy C,Lightcap E,Faustin B

    更新日期:2019-11-12 00:00:00

  • The role of Toll-like receptor-4 in pertussis vaccine-induced immunity.

    abstract:BACKGROUND:The gram-negative bacterium Bordetella pertussis is an important causative agent of pertussis, an infectious disease of the respiratory tract. After introduction of whole-cell vaccines (wP) in the 1950's, pertussis incidence has decreased significantly. Because wP were found to be reactogenic, in most develo...

    journal_title:BMC immunology

    pub_type: 杂志文章

    doi:10.1186/1471-2172-9-21

    authors: Banus S,Stenger RM,Gremmer ER,Dormans JA,Mooi FR,Kimman TG,Vandebriel RJ

    更新日期:2008-05-22 00:00:00

  • Residue analysis of a CTL epitope of SARS-CoV spike protein by IFN-gamma production and bioinformatics prediction.

    abstract:BACKGROUND:Severe acute respiratory syndrome (SARS) is an emerging infectious disease caused by the novel coronavirus SARS-CoV. The T cell epitopes of the SARS CoV spike protein are well known, but no systematic evaluation of the functional and structural roles of each residue has been reported for these antigenic epit...

    journal_title:BMC immunology

    pub_type: 杂志文章

    doi:10.1186/1471-2172-13-50

    authors: Huang J,Cao Y,Bu X,Wu C

    更新日期:2012-09-10 00:00:00

  • A selective and potent CXCR3 antagonist SCH 546738 attenuates the development of autoimmune diseases and delays graft rejection.

    abstract:BACKGROUND:The CXCR3 receptor and its three interferon-inducible ligands (CXCL9, CXCL10 and CXCL11) have been implicated as playing a central role in directing a Th1 inflammatory response. Recent studies strongly support that the CXCR3 receptor is a very attractive therapeutic target for treating autoimmune diseases, s...

    journal_title:BMC immunology

    pub_type: 杂志文章

    doi:10.1186/1471-2172-13-2

    authors: Jenh CH,Cox MA,Cui L,Reich EP,Sullivan L,Chen SC,Kinsley D,Qian S,Kim SH,Rosenblum S,Kozlowski J,Fine JS,Zavodny PJ,Lundell D

    更新日期:2012-01-10 00:00:00

  • In vitro and in vivo protective efficacies of antibodies that neutralize the RNA N-glycosidase activity of Shiga toxin 2.

    abstract:BACKGROUND:Shiga toxin 2 (Stx2), one of two Stx liberated by Stx-producing Escherichia coli, is composed of an A subunit monomer and a B subunit pentamer, and is directly linked with hemolytic uremic syndrome in children. The pentameric B subunit binds to its cell surface receptor Gb3 for toxin internalization, and the...

    journal_title:BMC immunology

    pub_type: 杂志文章

    doi:10.1186/1471-2172-11-16

    authors: Jeong KI,Chapman-Bonofiglio S,Singh P,Lee J,Tzipori S,Sheoran AS

    更新日期:2010-03-24 00:00:00

  • Activation of tonsil dendritic cells with immuno-adjuvants.

    abstract:BACKGROUND:Dendritic cells (DC) play the key role in directing antigen-specific immune responses and manipulating their function may be a useful tool for immunotherapy. The balance between immune stimulation and tolerance is particularly important at mucosal interfaces, where discrimination between dangerous pathogens ...

    journal_title:BMC immunology

    pub_type: 杂志文章

    doi:10.1186/1471-2172-9-10

    authors: Polak ME,Borthwick NJ,Gabriel FG,Jager MJ,Cree IA

    更新日期:2008-03-18 00:00:00

  • Mycobacterial glycolipids di-O-acylated trehalose and tri-O-acylated trehalose downregulate inducible nitric oxide synthase and nitric oxide production in macrophages.

    abstract:BACKGROUND:Tuberculosis (TB) remains a serious human health problem that affects millions of people in the world. Understanding the biology of Mycobacterium tuberculosis (Mtb) is essential for tackling this devastating disease. Mtb possesses a very complex cell envelope containing a variety of lipid components that par...

    journal_title:BMC immunology

    pub_type: 杂志文章

    doi:10.1186/s12865-015-0102-3

    authors: Espinosa-Cueto P,Escalera-Zamudio M,Magallanes-Puebla A,López-Marín LM,Segura-Salinas E,Mancilla R

    更新日期:2015-06-23 00:00:00

  • Insulin-like growth factor I promotes cord blood T cell maturation through monocytes and inhibits their apoptosis in part through interleukin-6.

    abstract:BACKGROUND:The functional immaturity of T cells contributes to the susceptibility of neonates to infections and the less severe graft-versus-host disease associated with cord blood (CB) transplantation. We have previously reported that insulin-like growth factor - I (IGF-I) promotes the phytohaemagglutinin (PHA)-induce...

    journal_title:BMC immunology

    pub_type: 杂志文章

    doi:10.1186/1471-2172-9-74

    authors: Law HK,Tu W,Liu E,Lau YL

    更新日期:2008-12-17 00:00:00

  • Tumor necrosis factor-alpha induced expression of matrix metalloproteinase-9 through p21-activated kinase-1.

    abstract:BACKGROUND:Expressed in embryonic development, matrix metalloprotein-9 (MMP-9) is absent in most of developed adult tissues, but recurs in inflammation during tissue injury, wound healing, tumor formation and metastasis. Expression of MMP-9 is tightly controlled by extracellular cues including pro-inflammatory cytokine...

    journal_title:BMC immunology

    pub_type: 杂志文章

    doi:10.1186/1471-2172-10-15

    authors: Zhou L,Yan C,Gieling RG,Kida Y,Garner W,Li W,Han YP

    更新日期:2009-03-19 00:00:00

  • Characteristics and reference ranges of CD4+T cell subpopulations among healthy adult Han Chinese in Shanxi Province, North China.

    abstract:BACKGROUND:Immunophenotyping of blood lymphocytes is an essential tool to evaluate the immune function of patients with immunodeficiency or autoimmunity. Predominately identified CD4+T cell subsets, Th1, Th2, Th17, as well as regulatory T (Treg) cells, play crucial roles in several immunological and pathological condit...

    journal_title:BMC immunology

    pub_type: 杂志文章

    doi:10.1186/s12865-020-00374-9

    authors: Niu HQ,Zhao XC,Li W,Xie JF,Liu XQ,Luo J,Zhao WP,Li XF

    更新日期:2020-08-03 00:00:00

  • Bacillus anthracis' lethal toxin induces broad transcriptional responses in human peripheral monocytes.

    abstract:BACKGROUND:Anthrax lethal toxin (LT), produced by the Gram-positive bacterium Bacillus anthracis, is a highly effective zinc dependent metalloprotease that cleaves the N-terminus of mitogen-activated protein kinase kinases (MAPKK or MEKs) and is known to play a role in impairing the host immune system during an inhalat...

    journal_title:BMC immunology

    pub_type: 杂志文章

    doi:10.1186/1471-2172-13-33

    authors: Chauncey KM,Lopez MC,Sidhu G,Szarowicz SE,Baker HV,Quinn C,Southwick FS

    更新日期:2012-07-02 00:00:00

  • Streptococcus pneumoniae synergizes with nontypeable Haemophilus influenzae to induce inflammation via upregulating TLR2.

    abstract:BACKGROUND:Toll-like receptor 2 (TLR2) plays a critical role in mediating inflammatory/immune responses against bacterial pathogens in lung. Streptococcus pneumoniae (S. pneumoniae) and nontypeable Haemophilus influenzae (NTHi) were previously reported to synergize with each other to induce inflammatory responses. Desp...

    journal_title:BMC immunology

    pub_type: 杂志文章

    doi:10.1186/1471-2172-9-40

    authors: Lim JH,Ha U,Sakai A,Woo CH,Kweon SM,Xu H,Li JD

    更新日期:2008-07-29 00:00:00

  • Association of proinflammatory genes expression with serum interleukin 1β and free fatty acids in metabolically healthy and unhealthy abdominally obese individuals: a case-control study.

    abstract:BACKGROUND:Proinflammatory genes are highly expressed in several metabolic disorders associated with obesity. But it is not clarified whether gene expression levels and downstream inflammatory markers are related to the metabolic state or the presence of obesity. Hence, the present study aimed to compare Toll-Like Rece...

    journal_title:BMC immunology

    pub_type: 杂志文章

    doi:10.1186/s12865-019-0303-2

    authors: Amiri P,Baradaran B,Saghafi-Asl M,Naghizadeh M,Shanehbandi D,Karamzad N,Vahed SZ

    更新日期:2019-07-04 00:00:00

  • Low-dose plasmid DNA treatment increases plasma vasopressin and regulates blood pressure in experimental endotoxemia.

    abstract:BACKGROUND:Although plasmid DNA encoding an antigen from pathogens or tumor cells has been widely studied as vaccine, the use of plasmid vector (without insert) as therapeutic agent requires further investigation. RESULTS:Here, we showed that plasmid DNA (pcDNA3) at low doses inhibits the production of IL-6 and TNF-α ...

    journal_title:BMC immunology

    pub_type: 杂志文章

    doi:10.1186/1471-2172-13-59

    authors: Malardo T,Batalhão ME,Panunto-Castelo A,Almeida LP,Padilha E,Fontoura IC,Silva CL,Carnio EC,Coelho-Castelo AA

    更新日期:2012-11-08 00:00:00