当前位置： SCI文献检索 > GENES & DEVELOPMENT期刊下所有文献 > A tetramer of the Flp recombinase silences the trimers within it during resolution of a Holliday junction substrate.

A tetramer of the Flp recombinase silences the trimers within it during resolution of a Holliday junction substrate.

Abstract：

:Recombination catalyzed by the Flp site-specific recombinase involves breakage and joining of four DNA strands between two target substrates. The reaction is carried out in two steps of pairwise strand exchanges by a DNA-protein assembly in which four Flp monomers act cooperatively to execute strand cleavage and joining. Two models for recombination have been proposed. In the trimer model, the two active sites required for each step are assembled from three Flp monomers. In the tetramer (or dimer of asymmetric dimers) model, the two active sites are assembled from four Flp monomers, two monomers each contributing one active site. Experiments in which the two models challenge each other reveal that, within the Flp tetramer arranged on a Holliday junction, the two active sites required for its resolution are derived from all four, rather than three, Flp monomers. Thus, the relative protein subunit configuration of the tetramer silences the trimers within it by excluding them from assembling a functional active site pair.

journal_name

Genes Dev

journal_title

Genes & development

authors

Lee J,Jayaram M

doi

10.1101/gad.11.18.2438

subject

Has Abstract

pub_date

1997-09-15 00:00:00

pages

2438-47

issue

18

eissn

0890-9369

issn

1549-5477

journal_volume

11

pub_type

杂志文章

相关文献

GENES & DEVELOPMENT文献大全
  • The PRC1 Polycomb group complex interacts with PLZF/RARA to mediate leukemic transformation.

    abstract：:Ectopic repression of retinoic acid (RA) receptor target genes by PML/RARA and PLZF/RARA fusion proteins through aberrant recruitment of nuclear corepressor complexes drives cellular transformation and acute promyelocytic leukemia (APL) development. In the case of PML/RARA, this repression can be reversed through trea...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.512009

    authors： Boukarabila H,Saurin AJ,Batsché E,Mossadegh N,van Lohuizen M,Otte AP,Pradel J,Muchardt C,Sieweke M,Duprez E

    更新日期：2009-05-15 00:00:00

  • Nucleocytoplasmic transport: the influenza virus NS1 protein regulates the transport of spliced NS2 mRNA and its precursor NS1 mRNA.

    abstract：:Influenza virus unspliced NS1 mRNA, like retroviral pre-mRNAs, is efficiently exported from the nucleus and translated in the cytoplasm of infected cells. With human immunodeficiency virus (HIV), the transport of viral pre-mRNAs is facilitated by the viral Rev protein. We tested the possibility that the influenza viru...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.6.2.255

    authors： Alonso-Caplen FV,Nemeroff ME,Qiu Y,Krug RM

    更新日期：1992-02-01 00:00:00

  • Life on the edge: telomeres and persistent DNA breaks converge at the nuclear periphery.

    abstract：:Persistent DNA double-strand breaks and telomeres represent genomic hazards, as they can instigate inappropriate repair reactions. Two recent papers by Oza and colleagues (pp. 912-917) and Schober and colleagues (pp. 928-938) show that both types of DNA ends are sequestered from bulk DNA by Mps3, a SUN domain protein ...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.1805309

    authors： Gartenberg MR

    更新日期：2009-05-01 00:00:00

  • An inducible change in Fox-1/A2BP1 splicing modulates the alternative splicing of downstream neuronal target exons.

    abstract：:Neuronal depolarization and CaM kinase IV signaling alter the splicing of multiple exons in transcripts for ion channels, neurotransmitter receptors, and other synaptic proteins. These splicing changes are mediated in part by special CaM kinase-responsive RNA elements, within or adjacent to exons that are repressed in...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.1837009

    authors： Lee JA,Tang ZZ,Black DL

    更新日期：2009-10-01 00:00:00

  • Expression of a dominant-negative Wnt blocks induction of MyoD in Xenopus embryos.

    abstract：:During gastrulation of Xenopus embryos the prospective mesoderm is induced initially with domains of dorsal and ventral fate, then further patterned to generate somitic mesoderm by signals from the gastrula organizer. Although Xwnt-8 is expressed in future ventrolateral mesoderm and induces prospective epidermis to di...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.10.21.2805

    authors： Hoppler S,Brown JD,Moon RT

    更新日期：1996-11-01 00:00:00

  • sel-10, a negative regulator of lin-12 activity in Caenorhabditis elegans, encodes a member of the CDC4 family of proteins.

    abstract：:Mutations that influence lin-12 activity in Caenorhabditis elegans may identify conserved factors that regulate the activity of lin-12/Notch proteins. We describe genetic evidence indicating that sel-10 is a negative regulator of lin-12/Notch-mediated signaling in C. elegans. Sequence analysis shows that SEL-10 is a m...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.11.23.3182

    authors： Hubbard EJ,Wu G,Kitajewski J,Greenwald I

    更新日期：1997-12-01 00:00:00

  • The cAMP receptor CAR4 regulates axial patterning and cellular differentiation during late development of Dictyostelium.

    abstract：:Pseudoplasmodia of developing Dictyostelium are organized with anteroposterior polarity. We have isolated CAR4, the gene for a new cell-surface, G protein-linked cAMP receptor. CAR4 mRNA is initially expressed during tip elongation and continues to accumulate into culmination. CAR4 is maximally expressed in pseudoplas...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.8.17.2086

    authors： Louis JM,Ginsburg GT,Kimmel AR

    更新日期：1994-09-01 00:00:00

  • Maternal Eed knockout causes loss of H3K27me3 imprinting and random X inactivation in the extraembryonic cells.

    abstract：:Genomic imprinting is essential for mammalian development. Recent studies have revealed that maternal histone H3 Lys27 trimethylation (H3K27me3) can mediate DNA methylation-independent genomic imprinting. However, the regulatory mechanisms and functions of this new imprinting mechanism are largely unknown. Here we dem...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.318675.118

    authors： Inoue A,Chen Z,Yin Q,Zhang Y

    更新日期：2018-12-01 00:00:00

  • Phosphatidic acid mediates demyelination in Lpin1 mutant mice.

    abstract：:Lipids play crucial roles in many aspects of glial cell biology, affecting processes ranging from myelin membrane biosynthesis to axo-glial interactions. In order to study the role of lipid metabolism in myelinating glial cells, we specifically deleted in Schwann cells the Lpin1 gene, which encodes the Mg2+-dependent ...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.1638008

    authors： Nadra K,de Preux Charles AS,Médard JJ,Hendriks WT,Han GS,Grès S,Carman GM,Saulnier-Blache JS,Verheijen MH,Chrast R

    更新日期：2008-06-15 00:00:00

  • MVH in piRNA processing and gene silencing of retrotransposons.

    abstract：:VASA is an evolutionarily conserved RNA helicase essential for germ cell development. The mouse PIWI family proteins MILI and MIWI2 are involved in production of Piwi-interacting RNAs (piRNAs) in fetal male germ cells through a ping-pong amplification cycle. Expression of retrotransposons is elevated in MILI- and MIWI...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.1902110

    authors： Kuramochi-Miyagawa S,Watanabe T,Gotoh K,Takamatsu K,Chuma S,Kojima-Kita K,Shiromoto Y,Asada N,Toyoda A,Fujiyama A,Totoki Y,Shibata T,Kimura T,Nakatsuji N,Noce T,Sasaki H,Nakano T

    更新日期：2010-05-01 00:00:00

  • The Nf2 tumor suppressor gene product is essential for extraembryonic development immediately prior to gastrulation.

    abstract：:The neurofibromatosis type II (NF2) tumor suppressor encodes a putative cytoskeletal associated protein, the loss of which leads to the development of Schwann cell tumors associated with NF2 in humans. The NF2 protein merlin belongs to the band 4.1 family of proteins that link membrane proteins to the cytoskeleton and...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.11.10.1253

    authors： McClatchey AI,Saotome I,Ramesh V,Gusella JF,Jacks T

    更新日期：1997-05-15 00:00:00

  • Cas6 is an endoribonuclease that generates guide RNAs for invader defense in prokaryotes.

    abstract：:An RNA-based gene silencing pathway that protects bacteria and archaea from viruses and other genome invaders is hypothesized to arise from guide RNAs encoded by CRISPR loci and proteins encoded by the cas genes. CRISPR loci contain multiple short invader-derived sequences separated by short repeats. The presence of v...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.1742908

    authors： Carte J,Wang R,Li H,Terns RM,Terns MP

    更新日期：2008-12-15 00:00:00

  • BMP/SMAD1 signaling sets a threshold for the left/right pathway in lateral plate mesoderm and limits availability of SMAD4.

    abstract：:Bistability in developmental pathways refers to the generation of binary outputs from graded or noisy inputs. Signaling thresholds are critical for bistability. Specification of the left/right (LR) axis in vertebrate embryos involves bistable expression of transforming growth factor beta (TGFbeta) member NODAL in the ...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.1682108

    authors： Furtado MB,Solloway MJ,Jones VJ,Costa MW,Biben C,Wolstein O,Preis JI,Sparrow DB,Saga Y,Dunwoodie SL,Robertson EJ,Tam PP,Harvey RP

    更新日期：2008-11-01 00:00:00

  • Role of the rasGAP-associated docking protein p62(dok) in negative regulation of B cell receptor-mediated signaling.

    abstract：:Antigenic stimulation of the B-cell receptor (BCR) is a central event in the immune response. In contrast, antigen bound to IgG negatively regulates signals from the BCR by cross-linking it to the inhibitory receptor FcgammaRIIB. Here we show that upon cross-linking of BCR or BCR with FcgammaRIIB, the rasGAP-associate...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：

    authors： Yamanashi Y,Tamura T,Kanamori T,Yamane H,Nariuchi H,Yamamoto T,Baltimore D

    更新日期：2000-01-01 00:00:00

  • cAMP stimulates the C/EBP-related transcription factor rNFIL-6 to trans-locate to the nucleus and induce c-fos transcription.

    abstract：:The c-fos serum response element (SRE) is a multifunctional regulatory region of the c-fos promoter that responds to a variety of inducers. Recently, we have demonstrated that the SRE binds the C/EBP-related transcription factor rat NFIL-6 (rNFIL-6). In this study we show that rNFIL-6 is regulated by the cAMP second m...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.5.10.1754

    authors： Metz R,Ziff E

    更新日期：1991-10-01 00:00:00

  • The fasciated ear2 gene encodes a leucine-rich repeat receptor-like protein that regulates shoot meristem proliferation in maize.

    abstract：:The ability to initiate organs throughout the lifecycle is a unique feature of plant development that is executed by groups of stem cells called meristems. The balance between stem cell proliferation and organ initiation is carefully regulated and ensures that organs can be initiated in regular geometric patterns. To ...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.208501

    authors： Taguchi-Shiobara F,Yuan Z,Hake S,Jackson D

    更新日期：2001-10-15 00:00:00

  • Hedgehog signaling and the retina: insights into the mechanisms controlling the proliferative properties of neural precursors.

    abstract：:Hedgehog signaling has been linked to cell proliferation in a variety of systems; however, its effects on the cell cycle have not been closely studied. In the vertebrate retina, Hedgehog's effects are controversial, with some reports emphasizing increased proliferation and others pointing to a role in cell cycle exit....

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.391106

    authors： Locker M,Agathocleous M,Amato MA,Parain K,Harris WA,Perron M

    更新日期：2006-11-01 00:00:00

  • Snail blocks the cell cycle and confers resistance to cell death.

    abstract：:The Snail zinc-finger transcription factors trigger epithelial-mesenchymal transitions (EMTs), endowing epithelial cells with migratory and invasive properties during both embryonic development and tumor progression. During EMT, Snail provokes the loss of epithelial markers, as well as changes in cell shape and the ex...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.294104

    authors： Vega S,Morales AV,Ocaña OH,Valdés F,Fabregat I,Nieto MA

    更新日期：2004-05-15 00:00:00

  • OBP100 binds remarkably degenerate octamer motifs through specific interactions with flanking sequences.

    abstract：:We have used the 100-kD HeLa cell octamer-binding protein OBP100 as a model to study flexible DNA sequence recognition by promoter-binding proteins. OBP100 binds to the conserved octamer motif ATGCAAAT found in numerous promoters and additionally to two degenerate octamer motifs (sites I and II) within the SV40 enhanc...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.2.11.1400

    authors： Baumruker T,Sturm R,Herr W

    更新日期：1988-11-01 00:00:00

  • A novel role for the 3' region of introns in pre-mRNA splicing of Saccharomyces cerevisiae.

    abstract：:To investigate the importance of sequences between the yeast (Saccharomyces cerevisiae) branch point (TACTAAC box) and 3' splice site (AG), we generated a series of pre-mRNA substrates that differed in the length of RNA retained on the 3' side of the TACTAAC box. These pre-mRNAs were compared as substrates for the fir...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.1.3.238

    authors： Rymond BC,Torrey DD,Rosbash M

    更新日期：1987-05-01 00:00:00

  • A Drosophila rRNA gene located in euchromatin is active in transcription and nucleolus formation.

    abstract：:P-element transformants of a single rRNA gene (rDNA) were used to investigate the relationship between the organization of the nucleolus organizer (NO) and rDNA function in Drosophila melanogaster. In situ hybridization to rRNA in polytene nuclei of salivary glands demonstrated that an rRNA gene can be transcribed at ...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.2.12b.1745

    authors： Karpen GH,Schaefer JE,Laird CD

    更新日期：1988-12-01 00:00:00

  • Genome-wide RNAi screen reveals a specific sensitivity of IRES-containing RNA viruses to host translation inhibition.

    abstract：:The widespread class of RNA viruses that utilize internal ribosome entry sites (IRESs) for translation include poliovirus and Hepatitis C virus. To identify host factors required for IRES-dependent translation and viral replication, we performed a genome-wide RNAi screen in Drosophila cells infected with Drosophila C ...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.1267905

    authors： Cherry S,Doukas T,Armknecht S,Whelan S,Wang H,Sarnow P,Perrimon N

    更新日期：2005-02-15 00:00:00

  • Brachyury downstream notochord differentiation in the ascidian embryo.

    abstract：:The ascidian tadpole represents the most simplified chordate body plan. It contains a notochord composed of just 40 cells, but as in vertebrates Brachyury is essential for notochord differentiation. Here, we show that the misexpression of the Brachyury gene (Ci-Bra) of Ciona intestinalis is sufficient to transform end...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.13.12.1519

    authors： Takahashi H,Hotta K,Erives A,Di Gregorio A,Zeller RW,Levine M,Satoh N

    更新日期：1999-06-15 00:00:00

  • The role of histone H2Av variant replacement and histone H4 acetylation in the establishment of Drosophila heterochromatin.

    abstract：:Activation and repression of transcription in eukaryotes involve changes in the chromatin fiber that can be accomplished by covalent modification of the histone tails or the replacement of the canonical histones with other variants. Here we show that the histone H2A variant of Drosophila melanogaster, H2Av, localizes ...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.1259105

    authors： Swaminathan J,Baxter EM,Corces VG

    更新日期：2005-01-01 00:00:00

  • Integrative genome analysis of somatic p53 mutant osteosarcomas identifies Ets2-dependent regulation of small nucleolar RNAs by mutant p53 protein.

    abstract：:TP53 is the most frequently mutated gene in human cancer. Many mutant p53 proteins exert oncogenic gain-of-function (GOF) properties that contribute to metastasis, but the mechanisms mediating these functions remain poorly defined in vivo. To elucidate how mutant p53 GOF drives metastasis, we developed a traceable som...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.304972.117

    authors： Pourebrahim R,Zhang Y,Liu B,Gao R,Xiong S,Lin PP,McArthur MJ,Ostrowski MC,Lozano G

    更新日期：2017-09-15 00:00:00

  • Purification and characterization of mSin3A-containing Brg1 and hBrm chromatin remodeling complexes.

    abstract：:Alteration of nucleosomes by ATP-dependent remodeling complexes represents a critical step in the regulation of transcription. The human SWI/SNF (hSWI/SNF) family is composed of complexes that contain either Brg1 or hBrm as the central ATPase; however, these separate complexes have not been compared functionally. Here...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.872801

    authors： Sif S,Saurin AJ,Imbalzano AN,Kingston RE

    更新日期：2001-03-01 00:00:00

  • pX, the HBV-encoded coactivator, suppresses the phenotypes of TBP and TAFII250 mutants.

    abstract：:Hepatitis B virus (HBV) infects humans and causes a wide range of clinical manifestations, from acute hepatitis to hepatocellular carcinoma (HCC). The HBV genome contains multiple promoters with gene expression regulated predominantly by the cellular transcription initiation machinery. Accordingly, the HBV-encoded pX,...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.12.8.1217

    authors： Haviv I,Matza Y,Shaul Y

    更新日期：1998-04-15 00:00:00

  • Cytokine rescue of p53-dependent apoptosis and cell cycle arrest is mediated by distinct Jak kinase signaling pathways.

    abstract：:Exposure of hematopoietic progenitors to gamma-irradiation (IR) induces p53-dependent apoptosis and a p53-independent G2/M cell cycle arrest. These responses to DNA-damage can be inhibited by treatment with cytokine growth factors. Here we report that gamma-IR-induced apoptosis and cell cycle arrest are suppressed by ...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.12.8.1099

    authors： Quelle FW,Wang J,Feng J,Wang D,Cleveland JL,Ihle JN,Zambetti GP

    更新日期：1998-04-15 00:00:00

  • Genome-wide RNAi analysis of JAK/STAT signaling components in Drosophila.

    abstract：:The cytokine-activated Janus kinase (JAK)/signal transducer and activator of transcription (STAT) pathway plays an important role in the control of a wide variety of biological processes. When misregulated, JAK/STAT signaling is associated with various human diseases, such as immune disorders and tumorigenesis. To gai...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.1320705

    authors： Baeg GH,Zhou R,Perrimon N

    更新日期：2005-08-15 00:00:00

  • Tel2 mediates activation and localization of ATM/Tel1 kinase to a double-strand break.

    abstract：:The kinases ATM and ATR (Tel1 and Mec1 in the yeast Saccharomyces cerevisiae) control the response to DNA damage. We report that S. cerevisiae Tel2 acts at an early step of the TEL1/ATM pathway of DNA damage signaling. We show that Tel1 and Tel2 interact, and that even when Tel1 protein levels are high, this interacti...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.1646208

    authors： Anderson CM,Korkin D,Smith DL,Makovets S,Seidel JJ,Sali A,Blackburn EH

    更新日期：2008-04-01 00:00:00