Abstract:
:Rats chronically exposed to acrylonitrile (ACN) have shown a dose-dependent increase in the incidence of astrocytomas in the brain. The mechanism(s) by which ACN induces cancer in rodents has not been established. ACN does not appear to be directly genotoxic in the brain and thus a nongenotoxic mode of action has been proposed. Inhibition of gap junctional intercellular communication (GJIC) has been shown to be a property of many nongenotoxic carcinogens. The present study examined the effects of ACN on GJIC in a rat astrocyte transformed cell line, DI TNC1 cells (a target cell for ACN carcinogenicity) and primary cultured hepatocytes (a nontarget cell for ACN carcinogenicity). ACN inhibited GJIC in rat astrocytes in a dose-dependent manner. Inhibition of GJIC was observed following 2 h treatment with 0.10 mmol/L and 1.00 mmol/L ACN. However, in primary cultured hepatocytes, ACN exposed did not result in inhibition of GJIC even after 48 h of continued treatment. In the astrocytes, GJIC inhibition plateaued after 4 h of treatment and remained blocked throughout the entire experimental period examined. Inhibition of GJIC in DI TNC1 cells was reversed by removal of ACN from the culture medium after 4 or 24 h of treatment. Cotreatment of astrocytes with vitamin E reduced the effect of ACN-induced inhibition of GJIC. Similarly, inhibition of GJIC was prevented by treatment with 2-oxothiazolidine-4-carboxylic acid (OTC), a precursor of glutathione synthesis. Decreasing cellular glutathione by treatment with buthionine sulfoxamine alone (without ACN) did not affect GJIC in astrocytes. Collectively, these results demonstrate that treatment with ACN caused a selective inhibition of GJIC in rat DI TNC1 astrocytes (the target cell type), but not in rat hepatocytes (a nontarget tissue). Inhibition of GJIC in astrocytes was reversed by treatment with antioxidants and suggests a potential role for oxidative stress in ACN-induced carcinogenesis.
journal_name
Cell Biol Toxicoljournal_title
Cell biology and toxicologyauthors
Kamendulis LM,Jiang J,Zhang H,deFeijter-Rupp H,Trosko JE,Klaunig JEdoi
10.1023/a:1007685504941subject
Has Abstractpub_date
1999-06-01 00:00:00pages
173-83issue
3eissn
0742-2091issn
1573-6822journal_volume
15pub_type
杂志文章abstract::Manganese exposure is among the many environmental risk factors linked to the progression of neurodegenerative diseases, such as manganese-induced parkinsonism. In animal models, chronic exposure to manganese causes loss of cell viability, neurodegeneration, and functional deficits. Polyamines, such as spermine, have ...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1007/s10565-018-09449-1
更新日期:2019-04-01 00:00:00
abstract::The use of normal adult liver hepatocytes in cell culture for biochemical, toxicological and pharmacological studies has been greatly limited owing to the loss of replicative capacity and differentiated liver function. This is contrary to the ability of the liver to regenerate following injury in vivo. This suggests t...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1007/BF00754467
更新日期:1993-10-01 00:00:00
abstract::Male albino rats with diabetes induced by the administration of streptozotocin (STZ) (45 mg/kg, i.v.) were treated with oral administration of diphenyl diselenide (DPDS) pre-dissolved in soya bean oil. A significant reduction in blood glucose levels was observed in STZ-induced diabetic rats treated with DPDS compared ...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1007/s10565-008-9095-5
更新日期:2009-08-01 00:00:00
abstract::Presently, a large effort is being made worldwide to increase the sustainability of industrial development, while preserving not only the quality of the environment but also that of animal and human life. In this work, sea urchin early developmental stages were used as a model to test the effects of the organophosphat...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1007/s10565-008-9061-2
更新日期:2008-12-01 00:00:00
abstract::In vitro directional migration of 10T1/2 fibroblasts is partially inhibited by TPA but not by its non-promoting analogues. Other tumor promoters, e.g., phenobarbital, saccharin, and benzoylperoxide had no measurable effect when added in concentrations known to affect in vitro two-step transformation or intercellular c...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1007/BF00120161
更新日期:1985-06-01 00:00:00
abstract::Drug-induced liver injury (DILI) is a leading cause of discontinuation of new drug approval or withdrawal of marketed medicine based on safety due to organ vulnerability. The aim of this research is to investigate the potential abilities of four different in vitro cell models (L-02, HepG2, HepaRG, and hiHeps cell line...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1007/s10565-016-9316-2
更新日期:2016-02-01 00:00:00
abstract::We present a simple, noninvasive, nondestructive all-purpose method for the quantitative evaluation of functional effects of xenobiotics with cultured cells and the work station for its routine, easy implementation. At present 1 to 150 cells growing in one to six dishes can be studied in parallel or otherwise at time ...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1007/BF00755775
更新日期:1994-12-01 00:00:00
abstract::One of the major objectives at the end of this century is the development of "alternative" tests for the evaluation of the pharmacological and/or toxicological activity of newly developed molecules. Contact allergy is no exception to the rule and many research programs have been started to develop in vitro techniques ...
journal_title:Cell biology and toxicology
pub_type: 杂志文章,评审
doi:10.1023/a:1007502723275
更新日期:1999-02-01 00:00:00
abstract::Mechanisms of cell death induced by toxins probably involve one or more processes such as inhibition of protein synthesis and impairment of plasma membrane integrity leading to an increase in membrane permeability. Since one of the possible actions of mastoparan, a cationic tetradecapeptide from wasp venom, is to pert...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1007/BF00135029
更新日期:1990-01-01 00:00:00
abstract::Cerebral infarction causes severe morbidity and mortality. Most patients with cerebral infarction should take antiplatelet drugs daily, so the effects of those drugs on the regeneration of the brain need to be investigated. Aspirin and clopidogrel are the most widely used antiplatelet drugs for the prevention of ische...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1007/s10565-017-9412-y
更新日期:2018-06-01 00:00:00
abstract::Various in vitro preparations have been utilized to study the cellular activity of vasoactive agents on renal cortical microvessels and on mesangial cells. The receptors and the transduction pathways of bradykinin and atrial natriuretic factor were characterized on cultured cortical vascular smooth muscle cells from t...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1007/BF00438157
更新日期:1996-12-01 00:00:00
abstract::Cellular accumulation of mono(2-ethylhexyl)phthalate (MEHP) has been recently demonstrated to disturb fat cell energy metabolism; however, the underlying mechanism remained unclear. The study aimed to determine how MEHP influenced fat cell transcriptome and how the changes might contribute to bioenergetics. Because of...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1007/s10565-016-9380-7
更新日期:2017-12-01 00:00:00
abstract::Inhibition of endosomal acidification disturbs insulin signaling in both liver and adipose cells. In this study we used MIN6 beta cells to determine whether bafilomycin, a potent inhibitor of the proton-translocating vacuolar ATPase, disrupts insulin signaling in islet beta cells. Pretreatment of MIN6 cells with varyi...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1007/s10565-006-0054-8
更新日期:2006-05-01 00:00:00
abstract::Harmful algal blooms (HABs) can have both lethal and sublethal impacts on shellfish. To understand the possible roles of haemocytes in bivalve immune responses to HABs and how the algae are affected by these cells (haemocytes), in vitro tests between cultured harmful algal species and haemocytes of the northern quahog...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1007/s10565-011-9186-6
更新日期:2011-08-01 00:00:00
abstract::The nephrotoxic fungal toxins ochratoxin A (OA), ochratoxin B (OB) and citrinin (CIT) are natural contaminants of foods and feeds. While cytotoxicity assays have proven useful for establishing relative toxicity and structure function relationships within groups of fungal toxins, a drawback of in vitro bioassays is the...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1023/a:1007581606944
更新日期:1998-10-01 00:00:00
abstract::Sister chromatid exchange rate was studied in 12 early diagnosed cases of nasopharyngeal carcinoma and in their paired controls. Exchange frequencies were also analyzed in 100 healthy subjects distributed in four regions of Hunan Province and correlated to nationality, age and sex. The incidence of sister chromatid ex...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1007/BF00117868
更新日期:1987-09-01 00:00:00
abstract::Based on the reduced expression of ethanol-oxidizing enzymes in human hepatocellular carcinoma (HepG2) cells, we analyzed the role of nonoxidative metabolites in ethanol-induced apoptosis in HepG2 cells. For this purpose, an analysis of volatile metabolites of ethanol using ion-mobility spectrometry and gas chromatogr...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1007/s10565-007-9009-y
更新日期:2007-11-01 00:00:00
abstract::The histopathological response and cell culture characteristics of liver cells from the R16 (grc-) strain of rats, which carries an MHC-linked deletion, were examined one week after a single intraperitoneal injection of 200 mg/kg body weight diethylnitrosamine (DEN) and were compared with the response of liver cells f...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1007/BF00249594
更新日期:1990-04-01 00:00:00
abstract::Echinoderms are valuable test species in marine ecotoxicology and offer a wide range of biological processes appropriate for this approach. Regenerating echinoderms can be regarded as amenable experimental models for testing the effects of exposure to contaminants, particularly endocrine disrupter compounds (EDCs). As...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1007/s10565-008-9057-y
更新日期:2008-12-01 00:00:00
abstract::The effects of aplysiatoxin and debromoaplysiatoxin on the clonal growth rate, cross-linked envelope formation and plasminogen activator secretion of normal human bronchial epithelial cells were studied. Neither compound was mitogenic over a wide range of concentrations (10(-13) to 10(-7)M). Both aplysiatoxin and debr...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1007/BF00125571
更新日期:1984-10-01 00:00:00
abstract::Although methylguanine-DNA-methyltransferase (MGMT) plays an important role in resistance to temozolomide (TMZ) in glioma, 40% of gliomas with MGMT inactivation are still resistant to TMZ. The underlying mechanism is not clear. Here, we report that forkhead box M1 (FoxM1) transcriptionally activates the expression of ...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1007/s10565-017-9381-1
更新日期:2017-12-01 00:00:00
abstract::During the last few years the increased presence of proinflammatory cytokines and oxidation products in Alzheimer's disease (AD) has been largely described. Oxysterols, formed by hydroxylation of cholesterol, occur naturally in the brain and are increased in the serum of AD patients. Of these oxysterols, 25-hydroxycho...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1023/a:1011996515328
更新日期:2001-01-01 00:00:00
abstract::Ten compounds representative of diverse classes of chemicals were evaluated for their cytotoxicity and transforming ability to human skin fibroblasts in vitro. Only five of the ten compounds were highly cytotoxic in the 0-100 micrograms/ml range and their order of cytotoxicity was: 2,5-bis(1-aziridinyl)-3,6-bis(carboe...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1007/BF00249592
更新日期:1990-04-01 00:00:00
abstract::The risk presented by mycotoxins is a toxicological problem. As the data given by physico-chemical analysis may be difficult to translate in terms of toxicity, however, especially when considering multiple contamination, we have developed a system for toxicological analysis of mycotoxins using cell cultures of differe...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1007/BF00122655
更新日期:1989-06-01 00:00:00
abstract::Oxidative cell injury could be induced by different reactive oxygen species (ROS) operating in multiple pathways. The present work is focused on three different models of oxidative stress: the xanthine/xanthine oxidase system (XXO), an extracellular superoxide anion generator; tert-butylhydroperoxide (TBHP), an analog...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1007/s10565-009-9120-3
更新日期:2010-04-01 00:00:00
abstract::Uranium is a natural radioactive metallic element; its effect on the organism is cumulative, and chronic exposure to this element can induce carcinogenesis. Three cities of the Amazon region-Monte Alegre, Prainha, and Alenquer-in North Brazil, are located in one of the largest uranium mineralization areas of the world...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1007/s10565-010-9152-8
更新日期:2010-10-01 00:00:00
abstract::Bisnaphthalimido compounds bis-intercalate to DNA via the major groove and are potentially potent cancer therapeutics. Previously, we incorporated natural polyamines as linkers connecting the two naphthalimido ring moieties to create a series of soluble bisnaphthalimidopropyl polyamines (BNIPPs). Here, extending earli...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1007/s10565-011-9199-1
更新日期:2011-12-01 00:00:00
abstract::Acute exacerbation of chronic obstructive pulmonary disease (AECOPD) as a serious event has high mortality and medical costs. Systemic inflammation and immune response are the major factors influencing the outcome and quality of patient with AECOPD. On basis of identification and validation of AECOPD-specific inflamma...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1007/s10565-017-9405-x
更新日期:2018-04-01 00:00:00
abstract::Sea urchin embryos and larvae represent suitable model systems on where to investigate the effects of heavy metals on development and cell viability. Here, we tested the toxic effects of low (10(-12 )M), medium (10(-9 )M), and high (10(-6 )M) cadmium chloride concentrations, mimicking unpolluted, moderately and highly...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1007/s10565-008-9066-x
更新日期:2008-12-01 00:00:00
abstract::Cationic lipids are widely used for gene transfer in vitro and show promise as vectors for in vivo gene therapy applications. However, there is limited understanding of the cellular mechanisms involved in nonviral gene transfer. We investigated two major steps that could be limiting barriers to cationic lipid-mediated...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1023/a:1007425803756
更新日期:1998-03-01 00:00:00