Two opposite effects of ATP on the apparent sensitivity of the cGMP-gated channel of the carp retinal cone.

Abstract:

:Effects of ATP on the activity of cGMP-gated channels from carp cone photoreceptors were studied. In 29% of the patches examined (N = 45), ATP (1 mM) enhanced a current evoked by cGMP (20 microM, up to about 100%), in 33%. ATP suppressed it by up to about 90%, and in the remaining 38%, ATP had no effect. ATP showed similar effects on a current evoked by 8-bromoguanosine 3',5'-cyclic monophosphate (2 microM, enhancing in 42% of the patches, suppressing in 25%, no effect in 33%, N = 12), suggesting that the effects were not through modulation of the phosphodiesterase. Both of the effects, enhancement and suppression, were produced by a change in apparent affinity for cGMP, since (1) the maximum current evoked by cGMP of the saturating concentration (> or = 1 mM) was not affected, and (2) the K1/2 value decreased by approximately 45% (N = 2) or increased by approximately 25% (N = 2). A lower pH (approximately 6) facilitated the enhancing effect. ATP-gamma-S (1 mM) showed a suppressing effect in 80% of the patches and no effect in 20% of the patches (N = 10). However, ATP-gamma-S did not show an enhancing effect. Thus, ATP had two opposite effects through different mechanisms on the apparent sensitivity of the channel to cGMP; increasing and decreasing.

journal_name

Vis Neurosci

journal_title

Visual neuroscience

authors

Watanabe S,Shen J

doi

10.1017/s0952523800012578

subject

Has Abstract

pub_date

1997-07-01 00:00:00

pages

609-15

issue

4

eissn

0952-5238

issn

1469-8714

pii

S0952523800012578

journal_volume

14

pub_type

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