当前位置： SCI文献检索 > CLINICAL AND EXPERIMENTAL IMMUNOLOGY期刊下所有文献 > Cytokine responses to the native and recombinant forms of the major surface glycoprotein of Pneumocystis carinii.

Cytokine responses to the native and recombinant forms of the major surface glycoprotein of Pneumocystis carinii.

Abstract：

:Pneumocystis carinii is a major opportunistic pathogen and leading cause of morbidity in patients with AIDS. The major surface glycoprotein (MSG) of P. carinii, represented by a family of related proteins encoded by unique genes, is highly immunogenic and contains T cell-protective epitopes. We undertook the present study to define the CD4 T helper (Th) response by cytokine secretion to native MSG and a recombinant form of the protein, MSG-B. Spleen cells were collected from Lewis rats and restimulated with both native MSG and MSG-B. Within 24 h, the CD4 cells secreted high levels of interferon-gamma (IFN-gamma) in response to both types of antigen, indicative of a Th1 response; however, after 72h of incubation, only the native MSG stimulated secretion of IL-4 (Th2 response) from the cells. We then investigated whether the presence of IL-4 could alter the predominant Th1 phenotype by the CD4 cells in response to MSG and MSG-B. Cells cultured with native MSG and IL-4 produced low levels of IFN-gamma and elevated levels of IL-4. Interestingly, cells incubated with MSG-B and IL-4 reduced production of IFN-gamma, but were not stimulated to produce increased levels of IL-4. The presence of anti-IFN-gamma antibody in the MSG- or MSG-B-stimulated cultures did not effect the expression of IFN-gamma mRNA, suggesting that the generation of Th1 cells in response to MSG or MSG-B was not dependent on IFN-gamma. We conclude that native MSG, which contains multiple forms of this antigen, and recombinant MSG elicit different cytokine responses in vitro. These data are not only important to studies of MSG, but may also be relevant to the role of MSG in the immunopathogenesis of P. carinii infection in vivo.

journal_name

Clin Exp Immunol

journal_title

Clinical and experimental immunology

authors

Theus SA,Smulian AG,Sullivan DW,Walzer PD

doi

10.1046/j.1365-2249.1997.4501348.x

subject

Has Abstract

pub_date

1997-08-01 00:00:00

pages

255-60

issue

2

eissn

0009-9104

issn

1365-2249

journal_volume

109

pub_type

杂志文章

相关文献

CLINICAL AND EXPERIMENTAL IMMUNOLOGY文献大全
  • Toll-like receptor (TLR) 4 polymorphisms are associated with a chronic course of sarcoidosis.

    abstract：:The aetiology of sarcoidosis, an inflammatory granulomatous multi-system disorder, is unclear. It is thought to be the product of an unknown exogenous antigenic stimulus and an endogenous genetic susceptibility. Toll-like receptors (TLR) are signal molecules essential for the cellular response to bacterial cell wall c...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2249.2006.03008.x

    authors： Pabst S,Baumgarten G,Stremmel A,Lennarz M,Knüfermann P,Gillissen A,Vetter H,Grohé C

    更新日期：2006-03-01 00:00:00

  • T lymphocyte function in hairy cell leukaemia.

    abstract：:The high incidence of infections characteristic of impaired cell-mediated immunity in patients with hairy cell leukemia led us to study T lymphocyte function in sixteen patients with the lymphocyte transformation test. All patients showed imparied responses to mitogens, attributable to one or more of the following cau...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：

    authors： Sabbe LJ,Meijer CJ,Jansen J

    更新日期：1980-11-01 00:00:00

  • NK cells, displaying early activation, cytotoxicity and adhesion molecules, are associated with mild dengue disease.

    abstract：:During the innate immune response against infections, Natural Killer (NK) cells are as important effector cells as are Cytotoxic T lymphocytes (CTL) generated after antigenic stimulation in the adaptative response. NK cells increase in numbers, after viral infection or vaccination. We investigated the NK cell and CD8 ...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2249.2006.02996.x

    authors： Azeredo EL,De Oliveira-Pinto LM,Zagne SM,Cerqueira DI,Nogueira RM,Kubelka CF

    更新日期：2006-02-01 00:00:00

  • Impaired interleukin (IL)-4-associated generation of CCR4-expressing T cells in neonates with hereditary allergy risk.

    abstract：:Reduced microbial exposure in early life may contribute to the increase of atopic diseases in 'westernized' societies but the underlying mechanisms remain elusive. The objective of this study was to examine how exposure to bacterial lipopolysaccharide (LPS) during early antigen encounter might influence the maturation...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2249.2005.02706.x

    authors： Haddeland U,Sletten GB,Brandtzaeg P,Nakstad B

    更新日期：2005-02-01 00:00:00

  • Cytokine mRNA expression in the liver of patients with primary biliary cirrhosis (PBC) and chronic hepatitis B (CHB).

    abstract：:The expression of cytokine mRNA species was determined in liver biopsies from six normal subjects, 18 patients with PBC and 14 patients with hepatitis B e antigen (HBeAg)-positive CHB using a reverse transcriptase-polymerase chain reaction (RT-PCR) technique. cDNA, obtained by reverse transcription using oligo d(T) pr...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：10.1046/j.1365-2249.1996.d01-759.x

    authors： Shindo M,Mullin GE,Braun-Elwert L,Bergasa NV,Jones EA,James SP

    更新日期：1996-08-01 00:00:00

  • Amyloid fibril protein AA in Papua New Guinean amyloidosis.

    abstract：:In this study of protein composition of amyloid fibrils isolated from eight patients representative of the spectrum of amyloidosis found in Papua New Guinea has been investigated. All fibril preparations, including three from patients with amyloidosis secondary to lepromatous leprosy and one from an unusual juvenile c...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：

    authors： Anders RF,Price MA,Wilkey IS,Husby G,Takitaki F,Natvig JB,McAdam KP

    更新日期：1976-04-01 00:00:00

  • Humoral and cellular immune response of the rat to immunization with bee venom.

    abstract：:Lewis rats were immunized with bee venom allergen in Freund's complete adjuvant (FCA) or with FCA only. Animals immunized with bee venom developed specific IgG antibodies but no specific IgE antibodies were detected. Lymphocytes from lymph nodes when cultured with antigen in vitro showed an increased stimulation index...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：

    authors： Schneider H,Urbanek R

    更新日期：1984-08-01 00:00:00

  • Immune response modulation by recombinant peptides expressed in virus-like particles.

    abstract：:Aspergillus fumigatus, a ubiquitous fungus, is implicated in the pathogenesis of a number of clinically different allergic diseases in man, including allergic bronchopulmonary aspergillosis. Peptide-based immunotherapy may offer an alternative treatment strategy for the management of allergic disease. The objective of...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：10.1046/j.1365-2249.2002.01776.x

    authors： Svirshchevskaya EV,Alekseeva L,Marchenko A,Viskova N,Andronova TM,Benevolenskii SV,Kurup VP

    更新日期：2002-02-01 00:00:00

  • In vitro production of IgE by human peripheral blood mononuclear cells. IV. Modulation by allergen of the spontaneous IgE antibody biosynthesis.

    abstract：:Peripheral blood lymphocytes (PBL) from a proportion of grass-sensitive patients, studied during or immediately after the grass pollination period, showed spontaneous production in vitro of grass-specific IgE antibody, whereas PBL from atopic patients sensitive to allergens other than grass pollens or non-atopic indiv...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：

    authors： Romagnani S,Maggi E,Del Prete GF,Almerigogna F,Biagiotti R,Giudizi MG,Ricci M

    更新日期：1982-07-01 00:00:00

  • Tetrandrine, a plant alkaloid, inhibits the production of tumour necrosis factor-alpha (cachectin) hy human monocytes.

    abstract：:Human mononuclear leucocytes (MNL) or the adherent fraction (monocytes) produced tumour necrosis factor-alpha (TNF-alpha) (by ELISA) in culture when stimulated with killed Staphylococcus aureus. The bisbenzylisoquinoline alkaloid, tetrandrine inhibited the capacity of MNL and monocytes to produce TNF-alpha at a concen...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2249.1990.tb05239.x

    authors： Ferrante A,Seow WK,Rowan-Kelly B,Thong YH

    更新日期：1990-05-01 00:00:00

  • Increased chemotaxis and activity of circulatory myeloid progenitor cells may contribute to enhanced osteoclastogenesis and bone loss in the C57BL/6 mouse model of collagen-induced arthritis.

    abstract：:Our study aimed to determine the functional activity of different osteoclast progenitor (OCP) subpopulations and signals important for their migration to bone lesions, causing local and systemic bone resorption during the course of collagen-induced arthritis in C57BL/6 mice. Arthritis was induced with chicken type II ...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：10.1111/cei.12862

    authors： Ikić Matijašević M,Flegar D,Kovačić N,Katavić V,Kelava T,Šućur A,Ivčević S,Cvija H,Lazić Mosler E,Kalajzić I,Marušić A,Grčević D

    更新日期：2016-12-01 00:00:00

  • Antibodies to a non-repeat region of Plasmodium falciparum antigen Pf155/RESA in individuals from malaria-endemic areas.

    abstract：:Human antibodies to the repeat regions of the Plasmodium falciparum asexual blood stage antigen Pf155/RESA interfere with parasite growth in vitro, but the significance in this respect of antibodies to non-repetitive epitopes is less clear. In this study the levels of antibodies to a non-repetitive part of Pf155/RESA ...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：10.1046/j.1365-2249.1999.00911.x

    authors： Siddique AB,Ahlborg N,Warsame M,Perlmann P,Berzins K

    更新日期：1999-06-01 00:00:00

  • Quantitative intracellular cytokine measurement: age-related changes in proinflammatory cytokine production.

    abstract：:The proinflammatory cytokines play a central role in mediating cellular and physiological responses, and levels may reflect immune system effectiveness. In this study, the effect of ageing on the inflammatory response was examined using a novel method to detect production of the proinflammatory cytokines, i.e. tumour ...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：10.1046/j.1365-2249.1998.00641.x

    authors： O'Mahony L,Holland J,Jackson J,Feighery C,Hennessy TP,Mealy K

    更新日期：1998-08-01 00:00:00

  • Multiple sclerosis: II. Effects of prothymosin alpha on the autologous and allogeneic MLR in patients with multiple sclerosis.

    abstract：:We have recently demonstrated that peripheral blood monocytes from patients with multiple sclerosis (MS) have a defect in stimulating autologous and allogeneic T lymphocytes. This defect was found to correlate with disease activity. In this report we demonstrate that prothymosin alpha (ProT alpha), a rat thymus fracti...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：

    authors： Reclos GJ,Baxevanis CN,Sfagos C,Papageorgiou C,Tsokos GC,Papamichail M

    更新日期：1987-11-01 00:00:00

  • Apoptosis of Th1-like cells in experimental tuberculosis (TB).

    abstract：:Th1 cell-induced anti-mycobacterial immunity is lost during a progressive Mycobacterium tuberculosis infection in a susceptible host. This study was designed to test the mechanism of the loss of anti-mycobacterial cell-mediated immune response. We demonstrate that M. tuberculosis infection results in increased Fas exp...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：10.1046/j.1365-2249.1999.00755.x

    authors： Das G,Vohra H,Saha B,Agrewala JN,Mishra GC

    更新日期：1999-02-01 00:00:00

  • Neutrophil phagocytosis and killing in insulin-dependent diabetes.

    abstract：:Neutrophil phagocytosis and killing of Candida albicans were examined using a radiometric assay in 25 patients with insulin-dependent diabetes and 17 controls under various in vitro metabolic conditions. Glucose was present at 5, 10 and 50 mM, beta-hydroxybutyrate at 1, 5 and 20 mM and glucose with beta-hydroxybutyrat...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：

    authors： Wilson RM,Reeves WG

    更新日期：1986-02-01 00:00:00

  • Immune responses to fractionated cytomegalovirus (CMV) antigens after HIV infection. Loss of cellular and humoral reactivity to antigens recognized by HIV-, CMV+ individuals.

    abstract：:In order to delineate the molecular pathogenesis of the increased susceptibility to CMV disease in HIV infection, the patterns of antigen responsiveness in HIV-infected and non-infected individuals were investigated. CMV was fractionated by SDS-PAGE and electroblotted onto nitrocellulose. Lymphoproliferative responses...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2249.1990.tb05490.x

    authors： Converse PJ,Fehniger TE,Ehrnst A,Strannegård O,Britton S

    更新日期：1990-12-01 00:00:00

  • In vitro reversal of cellular unresponsiveness induced by levamisole.

    abstract：:Mononuclear cells from twenty-one patients with depressed cellular reactivity were assessed for the ability to produce leucocyte inhibitory factor (LIF) and to transform after PHA stimulation, in the presence or absence of levamisole. Cells from nineteen patients failed to produce significant lymphokines when stimulat...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：

    authors： Golding H,Golding B,Jacobson R,Lomnitzer R,Koornhof HJ,Rabson AR

    更新日期：1976-11-01 00:00:00

  • A prospective controlled crossover trial of a new heat-treated intravenous immunoglobulin.

    abstract：:Twenty-one patients with primary immunoglobulin deficiency were enrolled in a crossover study to test the efficacy and safety of Alphaglobin in comparison with the licensed preparations Sandoglobulin and Gamimune. There was no statistical difference in these parameters between Alphaglobin and Sandoglobulin/Gamimune. T...

    journal_title：Clinical and experimental immunology

    pub_type： 临床试验,杂志文章

    doi：10.1111/j.1365-2249.1995.tb03465.x

    authors： Zuhrie SR,Webster AD,Davies R,Fay AC,Wallington TB

    更新日期：1995-01-01 00:00:00

  • Rabbit polymorphonuclear granulocyte function during ethanol administration--migration and oxidative responses in a joint with immune complex synovitis.

    abstract：:Functional impairments of polymorphonuclear granulocytes (PMN) are believed to contribute to hampered inflammation and host defence in alcoholics. We studied effects of i.v. ethanol administration on PMN responses in rabbits during induction of a knee-joint synovitis. The synovitis conferred systemic effects, since ch...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2249.1995.tb06647.x

    authors： Nilsson E,Thomsen P,Ericson L,Palmblad J

    更新日期：1995-10-01 00:00:00

  • Effects of recombinant granulocyte-colony stimulating factor administration during Mycobacterium avium infection in mice.

    abstract：:Granulocyte colony-stimulating factor (G-CSF) administration in vivo has been shown to improve the defence mechanisms against infection by different microbes. Here we evaluated a possible protective role of this molecule in a mouse model of mycobacterial infection. The administration of recombinant G-CSF promoted an e...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：10.1046/j.1365-2249.2001.01552.x

    authors： Gonçalves AS,Appelberg R

    更新日期：2001-05-01 00:00:00

  • Exercise stress alters murine lymphocyte subset distribution in spleen, lymph nodes and thymus.

    abstract：:Our previous work indicated that exercise stress in mice was associated with reduced splenic lymphocyte proliferation to T cell mitogens. The purpose of this study was to determine the effect of acute exercise stress and exercise training on the phenotype distribution of murine lymphocytes in the spleen, lymph nodes a...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：

    authors： Hoffman-Goetz L,Thorne R,Simpson JA,Arumugam Y

    更新日期：1989-05-01 00:00:00

  • Human TSLP directly enhances expansion of CD8+ T cells.

    abstract：:Human thymic stromal lymphopoietin (TSLP) promotes CD4(+) T-cell proliferation both directly and indirectly through dendritic cell (DC) activation. Although human TSLP-activated DCs induce CD8(+) T-cell proliferation, it is not clear whether TSLP acts directly on CD8(+) T cells. In this study, we show that human CD8(+...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2249.2008.03731.x

    authors： Akamatsu T,Watanabe N,Kido M,Saga K,Tanaka J,Kuzushima K,Nishio A,Chiba T

    更新日期：2008-10-01 00:00:00

  • Cell-mediated immunity in patients with chronic fatigue syndrome, healthy control subjects and patients with major depression.

    abstract：:The chronic fatigue syndrome (CFS) is characterized by severe persistent fatigue and neuropsychiatric symptoms. It has been proposed that the abnormalities in cell-mediated immunity which have been documented in patients with CFS may be attributable to a clinical depression, prevalent in patients with this disorder. C...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2249.1992.tb06416.x

    authors： Lloyd A,Hickie I,Hickie C,Dwyer J,Wakefield D

    更新日期：1992-01-01 00:00:00

  • The immunopathogenesis of fibrosis in systemic sclerosis.

    abstract：:Systemic sclerosis (SSc) is an idiopathic systemic autoimmune disease. It is characterized by a triad of hallmarks: immune dysfunction, fibrosis and vasculopathy. Immune dysfunction in SSc is characterized by the activation and recruitment of immune cells and the production of autoantibodies and cytokines. How immune ...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章,评审

    doi：10.1111/cei.13238

    authors： Brown M,O'Reilly S

    更新日期：2019-03-01 00:00:00

  • What does the future hold for clinical studies in vasculitis?

    abstract：:The era prior to 1990 was a time of careful observation of disease presentation, course, outcomes and meticulous pathology studies. These mainly single-centre studies introduced new life-saving therapies for drugs still used effectively today. In the 1970-1980s, cyclophosphamide (CyP) added to glucocorticosteroids (GC...

    journal_title：Clinical and experimental immunology

    pub_type： 历史文章,杂志文章,评审

    doi：10.1111/j.1365-2249.2011.04365.x

    authors： Hoffman GS

    更新日期：2011-05-01 00:00:00

  • Mechanism of a lymphocyte abnormality associated with HLA-B8/DR3: role of interleukin-1.

    abstract：:Lymphocytes from normal individuals with the histocompatibility antigens HLA-B8 and DR3 have impaired proliferative responses when stimulated with suboptimal concentrations of mitogens. We have previously shown that an important factor in the impaired response is a failure to produce normal quantities of interleukin-2...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2249.1990.tb05183.x

    authors： Hashimoto S,Michalski JP,Berman MA,McCombs C

    更新日期：1990-02-01 00:00:00

  • Contrasting activity of cytosin-guanosin dinucleotide oligonucleotides in mice with experimental colitis.

    abstract：:Intestinal inflammation in inflammatory bowel disease (IBD) and experimental models of colitis is characterized by a dysregulated intestinal immune response with elevated levels of Th1 cytokines. The luminal flora has been implicated as a major factor contributing to the initiation and perpetuation of inflammation in ...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：10.1046/j.1365-2249.2003.02288.x

    authors： Obermeier F,Dunger N,Strauch UG,Grunwald N,Herfarth H,Schölmerich J,Falk W

    更新日期：2003-11-01 00:00:00

  • Molecular basis for antibody cross-reactivity between the hepatitis C virus core protein and the host-derived GOR protein.

    abstract：:The presence of antibodies reactive to a recently cloned host-derived antigen GOR is highly correlated with the presence of antibodies to the hepatitis C virus (HCV). We explored the molecular basis for this observation, and address the following question: are antibodies reactive with GOR19-27 (QKAKSNPNR) a result of ...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2249.1994.tb06042.x

    authors： Zhang ZX,Chen M,Wallhagen K,Trojnar J,Magnius LO,Wahren B,Sällberg M

    更新日期：1994-06-01 00:00:00

  • Expression of killer inhibitory receptors on cytotoxic cells from HIV-1-infected individuals.

    abstract：:Dysfunction of cytotoxic activity of T and natural killer (NK) lymphocytes is a main immunological feature in patients with AIDS, but its basis are not well understood. It has been recently described that T and NK cell-mediated cytotoxicity can be regulated by HLA killer inhibitory receptors (KIR). In this work, we ha...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：10.1046/j.1365-2249.1999.00833.x

    authors： Galiani MD,Aguado E,Tarazona R,Romero P,Molina I,Santamaria M,Solana R,Peña J

    更新日期：1999-03-01 00:00:00