GABA(A) receptor-mediated miniature postsynaptic currents and alpha-subunit expression in developing cortical neurons.

Abstract:

:Previous studies have described maturational changes in GABAergic inhibitory synaptic transmission in the rodent somatosensory cortex during the early postnatal period. To determine whether alterations in the functional properties of synaptically localized GABA(A) receptors (GABA(A)Rs) contribute to development of inhibitory transmission, we used the whole cell recording technique to examine GABAergic miniature postsynaptic currents (mPSCs) in developing cortical neurons. Neurons harvested from somatosensory cortices of newborn mice showed a progressive, eightfold increase in GABAergic mPSC frequency during the first 4 wk of development in dissociated cell culture. A twofold decrease in the decay time of the GABAergic mPSCs, between 1 and 4 wk, demonstrates a functional change in the properties of GABA(A)Rs mediating synaptic transmission in cortical neurons during development in culture. A similar maturational profile observed in GABAergic mPSC frequency and decay time in cortical neurons developing in vivo (assessed in slices), suggests that these changes in synaptically localized GABA(A)Rs contribute to development of inhibition in the rodent neocortex. Pharmacological and reverse transcription-polymerase chain reaction (RT-PCR) studies were conducted to determine whether changes in subunit expression might contribute to the observed developmental alterations in synaptic GABA(A)Rs. Zolpidem (300 nM), a subunit-selective benzodiazepine agonist with high affinity for alpha1-subunits, caused a reversible slowing of the mPSC decay kinetics in cultured cortical neurons. Development was characterized by an increase in the potency of zolpidem in modulating the mPSC decay, suggesting a maturational increase in percentage of functionally active GABA(A)Rs containing alpha1 subunits. The relative expression of alpha1 versus alpha5 GABA(A)R subunit mRNA in cortical tissue, both in vivo and in vitro, also increased during this same period. Furthermore, single-cell RT-multiplex PCR analysis revealed more rapidly decaying mPSCs in individual neurons in which alpha1 versus alpha5 mRNA was amplified. Together these data suggest that changes in alpha-subunit composition of GABA(A)Rs contribute to the maturation of GABAergic mPSCs mediating inhibition in developing cortical neurons.

journal_name

J Neurophysiol

authors

Dunning DD,Hoover CL,Soltesz I,Smith MA,O'Dowd DK

doi

10.1152/jn.1999.82.6.3286

subject

Has Abstract

pub_date

1999-12-01 00:00:00

pages

3286-97

issue

6

eissn

0022-3077

issn

1522-1598

journal_volume

82

pub_type

杂志文章
  • Response properties of neurons in primary somatosensory cortex of owl monkeys reflect widespread spatiotemporal integration.

    abstract::Receptive fields of neurons in somatosensory area 3b of monkeys are typically described as restricted to part of a single digit or palm pad. However, such neurons are likely involved in integrating stimulus information from across the hand. To evaluate this possibility, we recorded from area 3b neurons in anesthetized...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.00709.2009

    authors: Reed JL,Qi HX,Zhou Z,Bernard MR,Burish MJ,Bonds AB,Kaas JH

    更新日期:2010-04-01 00:00:00

  • Hippocampal interneurons are excited via serotonin-gated ion channels.

    abstract::Hippocampal interneurons are excited via serotonin-gated ion channels. J. Neurophysiol. 78: 2493-2502, 1997. Serotonergic neurons of the median raphe nucleus heavily innervate hippocampal GABAergic interneurons located in stratum radiatum of area CA1, suggesting that this strong subcortical projection may modulate int...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.1997.78.5.2493

    authors: McMahon LL,Kauer JA

    更新日期:1997-11-01 00:00:00

  • Electrotonic length estimates in neurons with dendritic tapering or somatic shunt.

    abstract::1. Compartmental models were used to compute the time constants and coefficients of voltage and current transients in hypothetical neurons having tapering dendrites or soma shunt and in a serially reconstructed motoneuron with soma shunt. These time constants and coefficients were used in equivalent cylinder formulas ...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.1992.68.4.1421

    authors: Holmes WR,Rall W

    更新日期:1992-10-01 00:00:00

  • β2-Adrenergic receptor supports prolonged theta tetanus-induced LTP.

    abstract::The widespread noradrenergic innervation in the brain promotes arousal and learning by molecular mechanisms that remain largely undefined. Recent work shows that the β(2)-adrenergic receptor (β(2)AR) is linked to the AMPA-type glutamate receptor subunit GluA1 via stargazin and PSD-95 (Joiner ML, Lise MF, Yuen EY, Kam ...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.00374.2011

    authors: Qian H,Matt L,Zhang M,Nguyen M,Patriarchi T,Koval OM,Anderson ME,He K,Lee HK,Hell JW

    更新日期:2012-05-01 00:00:00

  • Behavioral inspiratory inhibition: inactivated and activated respiratory cells.

    abstract::1. Eleven adult cats were trained to stop inspiration in response to a conditioning stimulus. The conditioning stimuli were presented at the onset of inspiration at intervals of approximately 20-30 s. Intratracheal pressures, diaphragmatic activity, and the extracellular activity of single medullary respiratory neuron...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.1989.62.5.1069

    authors: Orem J

    更新日期:1989-11-01 00:00:00

  • Population encoding of spatial frequency, orientation, and color in macaque V1.

    abstract::1. We recorded local field potentials in the parafoveal representation in the primary visual cortex of anesthetized and paralyzed macaque monkeys with a multicontact electrode that provided for sampling of neural activity at 16 sites along a vertical penetration. Differential recordings at adjacent contacts were trans...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.1994.72.5.2151

    authors: Victor JD,Purpura K,Katz E,Mao B

    更新日期:1994-11-01 00:00:00

  • The two-dimensional spatial structure of simple receptive fields in cat striate cortex.

    abstract::1. A reverse correlation (6, 8, 25, 35) method is developed that allows quantitative determination of visual receptive-field structure in two spatial dimensions. This method is applied to simple cells in the cat striate cortex. 2. It is demonstrated that the reverse correlation method yields results with several desir...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.1987.58.6.1187

    authors: Jones JP,Palmer LA

    更新日期:1987-12-01 00:00:00

  • GIRK channel-mediated inhibition of melanin-concentrating hormone neurons by nociceptin/orphanin FQ.

    abstract::Targeting the melanin-concentrating hormone (MCH) system has been suggested as a potential treatment for obesity, anxiety disorders, as well as addiction. Despite the therapeutic potential of MCH agonists and antagonists, the endogenous factors regulating MCH activity, in particular those implicated in anxiety and rew...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.00791.2010

    authors: Parsons MP,Hirasawa M

    更新日期:2011-03-01 00:00:00

  • Motor unit control properties in constant-force isometric contractions.

    abstract::1. The purpose of this study was 1) to characterize the decrease observed in mean firing rates of motor units in the first 8-15 s of isometric constant-force contractions and 2) to investigate possible mechanisms that could account for the ability to maintain force output in the presence of decreasing motor unit firin...

    journal_title:Journal of neurophysiology

    pub_type: 临床试验,杂志文章

    doi:10.1152/jn.1996.76.3.1503

    authors: de Luca CJ,Foley PJ,Erim Z

    更新日期:1996-09-01 00:00:00

  • Regional specification of threshold sensitivity and response time in CBA/CaJ mouse spiral ganglion neurons.

    abstract::Previous studies of spiral ganglion neuron electrophysiology have shown that specific parameters differ according to cochlear location, with apical neurons being distinctly different from basal neurons. To align these features more precisely along the tonotopic axis of the cochlea, we developed a novel spiral ganglion...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.00284.2007

    authors: Liu Q,Davis RL

    更新日期:2007-10-01 00:00:00

  • Rapid adaptation to scaled changes of the mechanical environment.

    abstract::We investigated adaptation to simple force field scaling to determine whether the same strategy is used as during adaptation to more complex changes in the mechanical environment. Subjects initially trained in a force field, consisting of a rightward lateral force with a parabolic spatial profile (PF). The field stren...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.00269.2007

    authors: Hinder MR,Milner TE

    更新日期:2007-11-01 00:00:00

  • Linking express saccade occurance to stimulus properties and sensorimotor integration in the superior colliculus.

    abstract::Express saccades represent the fastest possible eye movements to visual targets with reaction times that approach minimum sensory-motor conduction delays. Previous work in monkeys has identified two specific neural signals in the superior colliculus (SC: a midbrain sensorimotor integration structure involved in gaze c...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.00047.2015

    authors: Marino RA,Levy R,Munoz DP

    更新日期:2015-08-01 00:00:00

  • Mechanisms within the spinal cord are involved in the movement-induced attenuation of an H reflex in the dog.

    abstract::1. H reflexes were elicited in the second interosseous muscle of the hindpaw of the anesthetized dog during passive rotation of the shank about the ipsilateral or contralateral knee. Reflexes sampled at four points in the cycle of movement were compared with stationary controls. For both the ipsilateral and contralate...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.1996.76.5.3589

    authors: Misiaszek JE,Barclay JK,Brooke JD

    更新日期:1996-11-01 00:00:00

  • Two-dimensional saccade-related population activity in superior colliculus in monkey.

    abstract::The two-dimensional distribution of population activity in the superior colliculus (SC) during saccadic eye movements in the monkey was estimated using radial basis functions. To make these ensemble activity estimates, cells in the deeper layers of the SC were recorded over much of the rostrocaudal (caudal to 3.8 mm f...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.1998.80.2.798

    authors: Anderson RW,Keller EL,Gandhi NJ,Das S

    更新日期:1998-08-01 00:00:00

  • Beside the point: motor adaptation without feedback-based error correction in task-irrelevant conditions.

    abstract::Adaptation of movement may be driven by the difference between planned and actual motor performance, or the difference between expected and actual sensory consequences of movement. To identify how the nervous system differentially uses these signals, we asked: does motor adaptation occur when movement errors are irrel...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.00273.2011

    authors: Schaefer SY,Shelly IL,Thoroughman KA

    更新日期:2012-02-01 00:00:00

  • Neural responses elicited by stimuli associated with masking of low-frequency, monaural tonal signals by noise.

    abstract::The responses of low-frequency, primary-like neurons of the ventral cochlear nucleus were measured as a function of signal-to-noise ratio for gated tonal signals and noise maskers that were varied in bandwidth and duration. For a given signal-to-noise ratio, each of the maskers is known to produce the same amount of m...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.1983.50.6.1516

    authors: Langford TL

    更新日期:1983-12-01 00:00:00

  • Motion information is spatially localized in a visual working-memory task.

    abstract::We asked if the information about stimulus motion used in a visual working-memory task is localized in space. Monkeys compared the directions of two moving random-dot stimuli, sample and test, separated by a temporal delay and reported whether the stimuli moved in the same or in different directions. By presenting the...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.2001.86.2.912

    authors: Zaksas D,Bisley JW,Pasternak T

    更新日期:2001-08-01 00:00:00

  • Development, form, and function of the mouse visual thalamus.

    abstract::The dorsal lateral geniculate nucleus (dLGN) of the thalamus is the exclusive relay of retinal information en route to the visual cortex. Although much of our understanding about dLGN comes from studies done in higher mammals, such as the cat and primate, the mouse as a model organism has moved to the forefront as a t...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章,评审

    doi:10.1152/jn.00651.2017

    authors: Guido W

    更新日期:2018-07-01 00:00:00

  • Histaminergic modulation of nonspecific plasticity of the auditory system and differential gating.

    abstract::In the auditory system of the big brown bat (Eptesicus fuscus), paired conditioned tonal (CS) and unconditioned leg stimuli (US) for auditory fear conditioning elicit tone-specific plasticity represented by best-frequency (BF) shifts that are augmented by acetylcholine, whereas unpaired CS and US for pseudoconditionin...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.00930.2011

    authors: Ji W,Suga N

    更新日期:2013-02-01 00:00:00

  • LTD at mossy fiber synapses onto stratum lucidum interneurons requires TrkB and retrograde endocannabinoid signaling.

    abstract::Hippocampal mossy fiber axons simultaneously activate CA3 pyramidal cells and stratum lucidum interneurons (SLINs), the latter providing feedforward inhibition to control CA3 pyramidal cell excitability. Filopodial extensions of giant boutons of mossy fibers provide excitatory synaptic input to the SLIN. These filopod...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.00669.2018

    authors: Pan E,Zhao Z,McNamara JO

    更新日期:2019-02-01 00:00:00

  • A cGMP-dependent protein kinase (PKG) controls synaptic transmission tolerance to acute oxidative stress at the Drosophila larval neuromuscular junction.

    abstract::Increasing evidence demonstrates that modulating the cGMP-dependent protein kinase G (PKG) pathway produces an array of behavioral phenotypes in the fruit fly, Drosophila melanogaster. Altering PKG activity, either genetically via the foraging (for) gene or using pharmacology modifies tolerance to acute abiotic stress...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.00784.2011

    authors: Caplan SL,Milton SL,Dawson-Scully K

    更新日期:2013-02-01 00:00:00

  • Defective sensorimotor integration in preparation for reaction time tasks in patients with multiple sclerosis.

    abstract::Slowness of voluntary movements in patients with multiple sclerosis (MS) may be due to various factors, including attentional and cognitive deficits, delays in motor conduction time, and impairment of specific central nervous system circuits. In 13 healthy volunteers and 20 mildly disabled, relapsing-remitting MS pati...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.00591.2014

    authors: Cabib C,Llufriu S,Casanova-Molla J,Saiz A,Valls-Solé J

    更新日期:2015-03-01 00:00:00

  • Human Na(v)1.8: enhanced persistent and ramp currents contribute to distinct firing properties of human DRG neurons.

    abstract::Although species-specific differences in ion channel properties are well-documented, little has been known about the properties of the human Nav1.8 channel, an important contributor to pain signaling. Here we show, using techniques that include voltage clamp, current clamp, and dynamic clamp in dorsal root ganglion (D...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.00113.2015

    authors: Han C,Estacion M,Huang J,Vasylyev D,Zhao P,Dib-Hajj SD,Waxman SG

    更新日期:2015-05-01 00:00:00

  • Thermoregulatory vasomotor tone of the rat tail and paws in thermoneutral conditions and its impact on a behavioral model of acute pain.

    abstract::The tail and paws in rodents are heat exchangers involved in the maintenance of core body temperature (T(core)). They are also the most widely used target organs to study acute or chronic "models" of pain. We describe the fluctuations of vasomotor tone in the tail and paws in conditions of thermal neutrality and the c...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.00721.2013

    authors: El Bitar N,Pollin B,Karroum E,Pincedé I,Mouraux A,Le Bars D

    更新日期:2014-11-01 00:00:00

  • Glycinergic miniature synaptic currents and receptor cluster sizes differ between spinal cord interneurons.

    abstract::The structural features of a synaptic connection between central neurons play an important role in determining the strength of the connection. In the present study, we have examined the relationship between the structural and functional properties of glycinergic synapses in the rat spinal cord. We have analyzed the st...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.1999.82.1.312

    authors: Oleskevich S,Alvarez FJ,Walmsley B

    更新日期:1999-07-01 00:00:00

  • Neurotransmitter regulation of the heart in the nudibranch Archidoris montereyensis.

    abstract::1. The heart of the nudibranch mollusc Archidoris montereyensis is uniquely responsive to regulation by identifiable cardiac motor neurons. The neurotransmitters mediating the strong excitatory and inhibitory actions of the neurons are unknown. 2. In this study we developed an infused, in vitro preparation of the Arch...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.1995.74.4.1639

    authors: Wiens BL,Brownell PH

    更新日期:1995-10-01 00:00:00

  • The inhibition of cat lateral superior olive unit excitatory responses to binaural tone bursts. II. The sustained discharges.

    abstract::1. Preliminary to extending a point process model of lateral superior olive (LSO) unit activity to describe the units' binaural responses, the statistical properties of their discharges to binaural tone bursts were studied. The hypothesis that stimulation of the contralateral ear results in the simple reduction of the...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.1988.59.1.184

    authors: Tsuchitani C

    更新日期:1988-01-01 00:00:00

  • Pain-facilitating medullary neurons contribute to opioid-induced respiratory depression.

    abstract::Respiratory depression is a therapy-limiting side effect of opioid analgesics, yet our understanding of the brain circuits mediating this potentially lethal outcome remains incomplete. Here we studied the contribution of the rostral ventromedial medulla (RVM), a region long implicated in pain modulation and homeostati...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.00563.2012

    authors: Phillips RS,Cleary DR,Nalwalk JW,Arttamangkul S,Hough LB,Heinricher MM

    更新日期:2012-11-01 00:00:00

  • Stance- and locomotion-dependent processing of vibration-induced proprioceptive inflow from multiple muscles in humans.

    abstract::We performed a whole-body mapping study of the effect of unilateral muscle vibration, eliciting spindle Ia firing, on the control of standing and walking in humans. During quiet stance, vibration applied to various muscles of the trunk-neck system and of the lower limb elicited a significant tilt in whole body postura...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.00764.2006

    authors: Courtine G,De Nunzio AM,Schmid M,Beretta MV,Schieppati M

    更新日期:2007-01-01 00:00:00

  • Both visual and idiothetic cues contribute to head direction cell stability during navigation along complex routes.

    abstract::Successful navigation requires a constantly updated neural representation of directional heading, which is conveyed by head direction (HD) cells. The HD signal is predominantly controlled by visual landmarks, but when familiar landmarks are unavailable, self-motion cues are able to control the HD signal via path integ...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.01041.2010

    authors: Yoder RM,Clark BJ,Brown JE,Lamia MV,Valerio S,Shinder ME,Taube JS

    更新日期:2011-06-01 00:00:00