Host cells of Toxoplasma gondii encystation in infected primary culture from mouse brain.

Abstract:

:In order to identify brain cell types that serve as host cells of Toxoplasma gondii encystation primary cultures from murine brain were infected and stained for neural and parasite stage-specific markers. In mixed culture inoculated with T. gondii tachyzoites, MAP2+ neurons, GFAP+ astrocytes, F4/80+ microglia, and O1+ oligodendrocytes proved to be infected as detected by parallel labeling of SAG1. At 4 days following infection with bradyzoites, cysts developed in neuronal, astroglial, and microglial host cells as clarified using bradyzoite-specific antibody 4F8. Additional staining of SAG1 revealed that astrocytes in bradyzoite-infected brain cell culture can also harbor tachyzoite-containing vacuoles. Stage conversion was observed shortly after inoculation and was accompanied by an increase in] parasite proliferation. However, tachyzoites became rare in prolonged culture. By contrast, the numbers of cysts and of the bradyzoites isolated multiplied during long-term culture. These findings demonstrate that both glial and neuronal host cells allow T. gondii encystation in the absence of T cell-derived cytokines and imply that a brain-internal spreading of bradyzoites may sustain chronic infection.

journal_name

Parasitol Res

journal_title

Parasitology research

authors

Fischer HG,Nitzgen B,Reichmann G,Gross U,Hadding U

doi

10.1007/s004360050311

subject

Has Abstract

pub_date

1997-01-01 00:00:00

pages

637-41

issue

7

eissn

0932-0113

issn

1432-1955

journal_volume

83

pub_type

杂志文章