Rapid chromosomal analysis of germ-line cells by FISH: an investigation of an infertile male with large-headed spermatozoa.

abstract：:Several studies have demonstrated an association between polycystic ovary syndrome (PCOS) and the dinucleotide repeat microsatellite marker D19S884, which is located in intron 55 of the fibrillin-3 (FBN3) gene. Fibrillins, including FBN1 and 2, interact with latent transforming growth factor (TGF)-beta-binding protein...

journal_title：Molecular human reproduction

authors： Prodoehl MJ,Hatzirodos N,Irving-Rodgers HF,Zhao ZZ,Painter JN,Hickey TE,Gibson MA,Rainey WE,Carr BR,Mason HD,Norman RJ,Montgomery GW,Rodgers RJ

更新日期：2009-12-01 00:00:00

abstract：:Tenascin-C is an extracellular matrix glycoprotein whose monomers include eight consecutive fibronectin type III-like repeats, encoded by exons 10-16, and which are subject to alternative splicing. Transcripts containing these exons are expressed during tissue wounding and active tissue remodelling. Human fetal membra...

journal_title：Molecular human reproduction

authors： Bell SC,Pringle JH,Taylor DJ,Malak TM

更新日期：1999-11-01 00:00:00

abstract：:Successful pregnancy in humans depends on deep invasion of the maternal decidua by extravillous trophoblast cells (EVTs), a process regulated by autocrine and paracrine signals in the decidual-trophoblast microenvironment. Here we examined whether trophoblast invasion is affected by decidual differentiation of endomet...

journal_title：Molecular human reproduction

authors： Gonzalez M,Neufeld J,Reimann K,Wittmann S,Samalecos A,Wolf A,Bamberger AM,Gellersen B

更新日期：2011-07-01 00:00:00

abstract：:We have investigated the possible roles of oncostatin M (OSM), which is a member of the interleukin-6 family of cytokines, in endometrial and endometriotic stromal cell growth. Endometrial and endometriotic stromal cells were collected from the uterus or ovarian chocolate cysts. We observed the expression of mRNA tran...

journal_title：Molecular human reproduction

authors： Ohata Y,Harada T,Fujii A,Yoshida S,Iwabe T,Terakawa N

更新日期：2001-07-01 00:00:00

abstract：:Fetal membranes overlying the cervix in patients prior to and during labour, and within the rupture tear after spontaneous delivery at term, exhibit altered morphology. In this study we report that in comparison to mid-zone fetal membranes biopsies, these regions are characterized by increased expression of the matric...

journal_title：Molecular human reproduction

authors： McParland PC,Bell SC,Pringle JH,Taylor DJ

更新日期：2001-05-01 00:00:00

abstract：:To mimic post-ovulatory ageing, we have extended the in vitro maturation (IVM) phase to 48 h and examined effects on (i) developmental potential, (ii) expression of a panel of developmentally important genes and (iii) gene-specific epigenetic marks. Results were compared with the 24 h IVM protocol (control) usually em...

journal_title：Molecular human reproduction

authors： Heinzmann J,Mattern F,Aldag P,Bernal-Ulloa SM,Schneider T,Haaf T,Niemann H

更新日期：2015-10-01 00:00:00

abstract：:During early gestation, a considerable increase in different leukocyte subsets can be observed in the decidualized endometrium concomitantly to the invasion of cytotrophoblast cells (CTB). To date, it is still in question which factors induce this accumulation of immune cells and whether it is evoked by an in situ pro...

journal_title：Molecular human reproduction

authors： Segerer SE,Martignoni F,Bogdan A,Müller N,Kapp M,Dietl J,Rieger L,Kämmerer U

更新日期：2013-06-01 00:00:00

abstract：:Ovaries surgically removed for fertility preservation from a total of 24 women served as a source of human small antral follicles, including the follicular fluid (FF) and the corresponding granulosa cells (GC). The FF was used to evaluate the intrafollicular concentrations of anti-Müllerian hormone (AMH), inhibin-B, e...

journal_title：Molecular human reproduction

authors： Eilsø Nielsen M,Rasmussen IA,Fukuda M,Westergaard LG,Yding Andersen C

更新日期：2010-09-01 00:00:00

abstract：:Oxidative damage by reactive oxygen species (ROS) is a major cause of sperm dysfunction. Excessive ROS generation reduces fertilization and enhances DNA damage of spermatozoa. Interaction between spermatozoa and oviductal epithelial cells improves the fertilizing ability of and reduces chromatin damage in spermatozoa....

journal_title：Molecular human reproduction

authors： Huang VW,Lee CL,Lee YL,Lam KK,Ko JK,Yeung WS,Ho PC,Chiu PC

更新日期：2015-06-01 00:00:00

abstract：:Etoposide is widely used in the treatment of patients with testicular cancer. The mechanism underlying apoptosis induction in cancer cells has been studied in different cell types, but it is not known whether the same factors participate in viable germ cells undergoing programmed cell death. Since testicular cancer pr...

journal_title：Molecular human reproduction

authors： Ortiz RJ,Lizama C,Codelia VA,Moreno RD

更新日期：2009-06-01 00:00:00

abstract：:We describe a rapid and specific differential amplification system which can detect five of the most common cystic fibrosis mutations from a single cell. In the first round of the polymerase chain reaction (PCR), regions of exons 4, 10 and 11 of the cystic fibrosis transmembrane conductance regulator (CFTR) gene conta...

journal_title：Molecular human reproduction

authors： Scobie G,Woodroffe B,Fishel S,Kalsheker N

更新日期：1996-03-01 00:00:00

abstract：:In male mice heterozygous for a null apolipoprotein B (apoB), allele infertility was noticed. These data led us to investigate a possible role of APOB gene polymorphism and male infertility in humans. In this case-control study, we searched for an association between the insertion/deletion (I/D) polymorphism of the AP...

journal_title：Molecular human reproduction

authors： Peterlin B,Zorn B,Volk M,Kunej T

更新日期：2006-12-01 00:00:00

abstract：:We have previously demonstrated that the reduced form of vitamin C (l-ascorbic acid, AA) is able to induce the production of both steroid and peptide hormones in human choriocarcinoma cells. Here, we attempted to investigate the role and underlying mechanism of the oxidized form of vitamin C, dehydroascorbic acid (DHA...

journal_title：Molecular human reproduction

authors： Wang Y,Tang C,Wu M,Pan Y,Ruan H,Chen L,Yao H,Zhu H,Wu X

更新日期：2014-08-01 00:00:00

abstract：:Ascorbic acid serves a vital role as a pre-eminent antioxidant. In animals, it has been shown to be concentrated in granulosa and theca cells of the follicle, in luteal cells of the corpus luteum, and in the peripheral cytoplasm of the oocyte. We have previously identified hormonally-regulated ascorbic acid transporte...

journal_title：Molecular human reproduction

authors： Zreik TG,Kodaman PH,Jones EE,Olive DL,Behrman H

更新日期：1999-04-01 00:00:00

abstract：:The use of interphase fluorescent in situ hybridization (FISH) has shown that a large number of human embryos exhibit chromosomal abnormalities in vitro. The most common abnormality is mosaicism which is seen in up to 50% of preimplantation embryos at all stages of development. In this study, comparative genomic hybri...

journal_title：Molecular human reproduction

authors： Daphnis DD,Fragouli E,Economou K,Jerkovic S,Craft IL,Delhanty JD,Harper JC

更新日期：2008-02-01 00:00:00

abstract：STUDY QUESTION:Could a more detailed evaluation of marmoset spermatogonial morphology, kinetics and niches using high-resolution light microscopy (HRLM) lead to new findings? SUMMARY ANSWER:Three subtypes of marmoset undifferentiated spermatogonia, which were not evenly distributed in terms of number and position alon...

journal_title：Molecular human reproduction

authors： Caldeira-Brant AL,Eras-Garcia L,Alves-Freitas D,Almeida FRCL,Chiarini-Garcia H

更新日期：2018-06-01 00:00:00

abstract：:Acrosome status in human spermatozoa from 20 normozoospermic men was evaluated by flow cytometry following the induction of the acrosome reaction with the ionophore A23187. Dual fluorescence staining of methanol fixed spermatozoa incubated with and without (control) the ionophore A23187 was performed with probes which...

journal_title：Molecular human reproduction

authors： Nikolaeva MA,Golubeva EL,Kulakov VI,Sukhikh GT

更新日期：1998-03-01 00:00:00

abstract：:A number of placental cytokines participate in the feto-maternal defence mechanism. Monocyte chemotactic and activating factor (MCAF) is one of these chemokines. We investigated the pattern of placental MCAF production, the localization of MCAF-producing cells in the placenta, and alterations in its expression in chor...

journal_title：Molecular human reproduction

authors： Shimoya K,Matsuzaki N,Sawai K,Saji F,Murata Y,Yasumoto K,Su S,Mukaida N,Matsushima K

更新日期：1998-04-01 00:00:00

abstract：:Ever since work on pluripotency induction was originally published, reporting the reprogramming of somatic cells to induced pluripotent stem cells (iPS cells) by the ectopic expression of the four transcription factors Oct4, Sox2, Klf4 and c-Myc, high expectations regarding their potential use for regenerative medicin...

journal_title：Molecular human reproduction

authors： Masip M,Veiga A,Izpisúa Belmonte JC,Simón C

更新日期：2010-11-01 00:00:00

abstract：STUDY QUESTION:Which different pathways and functions are altered in rhesus monkey oocytes that fail to mature after an ovulatory stimulus? SUMMARY ANSWER:Failed to mature (FTM) oocytes complete a large portion of the transition in transcriptome composition associated with normal maturation, but also manifest numerous...

journal_title：Molecular human reproduction

authors： Ruebel ML,Schall PZ,Midic U,Vincent KA,Goheen B,VandeVoort CA,Latham KE

更新日期：2018-10-01 00:00:00

abstract：:G12 monoclonal antibody (mAb), one of a library of constructed mAb directed against human sperm proteins, was found by immunoperoxidase staining to label the post-acrosomal and neck regions of fixed human cauda epididymal and ejaculated spermatozoa. Epithelium and fluid of caput epididymis were strongly labelled while...

journal_title：Molecular human reproduction

authors： Lefèvre A,Martin Ruiz C,Chokomian S,Duquenne C,Finaz C

更新日期：1997-06-01 00:00:00

abstract：:The reproductive lifespan of a woman is determined by the gradual recruitment of quiescent follicles into the growing pool. In humans, ovarian tissue removal from its in vivo environment induces spontaneous activation of resting follicles. Similarly, pharmacological activation of the PI3K/Akt pathway leads to accelera...

journal_title：Molecular human reproduction

authors： Grosbois J,Vermeersch M,Devos M,Clarke HJ,Demeestere I

更新日期：2019-11-30 00:00:00

abstract：:Follicular development and ovulation are major processes in the reproductive system. Understanding their complexity is important to female fertility treatments and the control of reproductive processes. Wnt signaling pathway components were shown to be involved in reproduction in animal models. The secreted frizzled-r...

journal_title：Molecular human reproduction

authors： Maman E,Yung Y,Cohen B,Konopnicki S,Dal Canto M,Fadini R,Kanety H,Kedem A,Dor J,Hourvitz A

更新日期：2011-07-01 00:00:00

abstract：:Recurrent pregnancy loss (RPL) is a common and distressing disorder. Chromosomal errors in the embryo are the single most common cause, whereas uterine factors are invariably invoked to explain non-chromosomal miscarriages. These uterine factors are, however, poorly defined. The ability of a conceptus to implant in th...

journal_title：Molecular human reproduction

authors： Teklenburg G,Salker M,Heijnen C,Macklon NS,Brosens JJ

更新日期：2010-12-01 00:00:00

abstract：:Mammalian sperm undergo a series of maturation steps before acquiring fertilization competence. Our previous work demonstrated the importance of binder of sperm (BSP) proteins in bovine sperm capacitation. Recent studies identified a BSP-homologous DNA sequence in the human genome (BSPH1) and mRNA expression in the ep...

journal_title：Molecular human reproduction

authors： Lefebvre J,Boileau G,Manjunath P

更新日期：2009-02-01 00:00:00

abstract：:The present study was undertaken to investigate the effect of decidualization on superoxide dismutase (SOD) expression in human endometrial stromal cells (ESC). To induce decidualization, isolated ESC were incubated with medroxyprogesterone acetate (MPA, 10(-6) mol/l) and oestradiol (10(-8) mol/l) for 23 days. Insulin...

journal_title：Molecular human reproduction

authors： Sugino N,Kashida S,Takiguchi S,Nakamura Y,Kato H

更新日期：2000-02-01 00:00:00

abstract：:Polar body emission is a specialized cell division throughout the animal kingdom, serving to reduce chromosome ploidy while preserving the egg cytoplasm. Critical to polar body emission are the asymmetric positioning of the meiotic spindle prior to anaphase, with one pole attached to the oocyte cortex, and the simulta...

journal_title：Molecular human reproduction

authors： Leblanc J,Zhang X,McKee D,Wang ZB,Li R,Ma C,Sun QY,Liu XJ

更新日期：2011-05-01 00:00:00

abstract：:The clinical application of quantitative fluorescent polymerase chain reaction (QF-PCR) for rapid prenatal detection of chromosome aneuploidies has been limited in most studies to the detection of autosomal trisomies. Recently it has been shown that a newly identified highly polymorphic marker, termed X22, which maps ...

journal_title：Molecular human reproduction

authors： Cirigliano V,Ejarque M,Cañadas MP,Lloveras E,Plaja A,Perez MM,Fuster C,Egozcue J

更新日期：2001-10-01 00:00:00

abstract：:The identification of polymorphisms in genes encoding proinflammatory cytokines that affect transcription or the secretion rate has opened new ways to understand the variation in responses to infection during pregnancy. In this study, human amniochorion carrying hyper-responsive alleles of tumour necrosis factor-alpha...

journal_title：Molecular human reproduction

authors： Hernandez-Guerrero C,Monzon-Bordonaba F,Jimenez-Zamudio L,Ahued-Ahued R,Arechavaleta-Velasco F,Strauss JF 3rd,Vadillo-Ortega F

更新日期：2003-10-01 00:00:00

abstract：:Chemokines are implicated in the implantation process. The aim of this study was to investigate mRNA expression and protein levels of chemokine receptors CXCR1, CXCR4, CCR5 and CCR2B in human endometrium throughout the menstrual cycle, during HRT and in the human blastocyst. The regulation of chemokine receptors in th...

journal_title：Molecular human reproduction

authors： Dominguez F,Galan A,Martin JJ,Remohi J,Pellicer A,Simón C

更新日期：2003-04-01 00:00:00