CD11b/CD18-coated microspheres attach to E-selectin under flow.

Abstract:

:Neutrophils can attach to E-selectin under flow. Proposed ligands for E-selectin carry SLe(x)-type glycans. The leukocyte beta2 integrins are glycosylated with SLe(x). Thus, we speculated that beta2 integrins could support attachment to E-selectin. To test this hypothesis, we coated 10-microm-diameter microspheres with purified CD11b/CD18 (alphaMbeta2) and investigated the adhesion of the resulting alphaMbeta2 microspheres to E-selectin. Under in vitro flow conditions, the alphaMbeta2 microspheres attached to Chinese hamster ovary cells expressing E-selectin (CHO-E) and 4-h interleukin-1beta-activated human umbilical vein endothelial cells (HUVEC). At a shear stress of 1.8 dynes/cm2, the attachment events were eliminated by pretreatment of the cellular monolayers with a mAb to E-selectin. alphaMbeta2 microspheres did not attach to untransfected CHO cells or unactivated HUVEC at 1.8 dynes/cm2. Taken together, the results strongly suggest that the CD11b/CD18-E-selectin bond has sufficient biophysical properties to mediate attachment of neutrophil-sized particles to E-selectin under flow.

journal_name

J Leukoc Biol

authors

Crutchfield KL,Shinde Patil VR,Campbell CJ,Parkos CA,Allport JR,Goetz DJ

doi

10.1002/jlb.67.2.196

subject

Has Abstract

pub_date

2000-02-01 00:00:00

pages

196-205

issue

2

eissn

0741-5400

issn

1938-3673

journal_volume

67

pub_type

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