Potential of electrospray mass spectrometry for quantifying glycohemoglobin.

Abstract:

:An electrospray ionization-mass spectrometric procedure has been developed for determining glycohemoglobin. Whole-blood samples from 78 diabetic and 50 nondiabetic subjects (glycation range 3-15%, as determined by electrospray mass spectrometry) were diluted 500-fold in an acidic denaturing solvent and introduced directly into a mass spectrometer. The resulting mass spectra were then processed to estimate the percentage of glycohemoglobin present in the sample. Total analysis time, including plotting the spectra and computing the percentage of glycation, was approximately 3 min. The imprecision (CV) of the method was < 5.1% for inter- and intrabatch analyses for total glycohemoglobin in the range 3.6-14%. Comparison of the mass spectrometric results with those from established affinity chromatographic procedures showed good overall agreement. The relative glycation of the alpha- and beta-chains was determined directly and was shown to be constant (0.64:1) over the glycation range measured. Only single glucose attachment to both the alpha- and beta-chains was observed.

journal_name

Clin Chem

journal_title

Clinical chemistry

authors

Roberts NB,Green BN,Morris M

subject

Has Abstract

pub_date

1997-05-01 00:00:00

pages

771-8

issue

5

eissn

0009-9147

issn

1530-8561

journal_volume

43

pub_type

杂志文章
  • Enzymatic determination of serum urea on the surface of silicone-rubber pads.

    abstract::An enzymatic, fluorometric method is described for determination of serum urea on silicone-rubber pads. In this method, the reagents are lyophilized on the surface of the pads, NADH on one side and a mixture of urease, glutamate dehydrogenase, and alpha-ketoglutarate on the other. The rate of disappearance of NADH flu...

    journal_title:Clinical chemistry

    pub_type: 杂志文章

    doi:

    authors: Kuan JC,Lau KY,Guilbault GG

    更新日期:1975-01-01 00:00:00

  • Improved liquid chromatographic/immunoassay of digoxin in serum.

    abstract::This HPLC/immunoassay procedure measures digoxin in serum with no interference from digoxin metabolites or digoxin-like factors. We used solid-phase (C18 and Diol) extraction, a C18 column with a tetrahydrofuran/water mobile phase, and final quantification by fluorescence polarization immunoassay. Deslanoside and gito...

    journal_title:Clinical chemistry

    pub_type: 杂志文章

    doi:

    authors: Stone JA,Soldin SJ

    更新日期:1988-12-01 00:00:00

  • Liquid-chromatographic measurement of amino acids in biological samples after formation of phenylthiohydantoin derivatives.

    abstract::In this method for measuring amino acids in urine, serum, and tissue, the amino acids to be assayed and the internal standard, L-norleucine, are converted to phenylthiohydantoins , isolated by organic solvent extraction, separated by reversed-phase "high-pressure" liquid chromatography, and detected by ultraviolet abs...

    journal_title:Clinical chemistry

    pub_type: 杂志文章

    doi:

    authors: Biggs HG,Gentilcore LJ

    更新日期:1984-06-01 00:00:00

  • Emergency gas-chromatographic assay of phenobarbital and phenytoin and liquid-chromatographic assay of theophylline.

    abstract::Phenobarbital and phenytoin are extracted and concentrated in a single step, without solvent evaporation, followed by on-column methylation and gas-chromatographic quanitation. A similar extraction step for the assay of theophylline by high-pressure liquid chromatography is described. The extraction step can be comple...

    journal_title:Clinical chemistry

    pub_type: 杂志文章

    doi:

    authors: Shihabi ZK

    更新日期:1978-09-01 00:00:00

  • C-reactive protein uptake by macrophage cell line via class-A scavenger receptor.

    abstract:BACKGROUND:C-reactive protein (CRP) increases in response to inflammation and is purported to be a risk factor for atherogenesis. We recently demonstrated that a scavenger receptor, lectin-like oxidized LDL receptor (LOX-1), is a receptor for CRP. In light of the overlapping ligand spectrum of scavenger receptors such ...

    journal_title:Clinical chemistry

    pub_type: 杂志文章

    doi:10.1373/clinchem.2009.140202

    authors: Fujita Y,Kakino A,Harada-Shiba M,Sato Y,Otsui K,Yoshimoto R,Sawamura T

    更新日期:2010-03-01 00:00:00

  • A technique for determining the probability of abnormality.

    abstract::Histograms of the results of quantitative clinical laboratory analyses are usually broadly skewed because some of the patients have normal values with respect to the analyte being measured while values for others are below or above normal. We describe a technique for calculating the maximum possible number of individu...

    journal_title:Clinical chemistry

    pub_type: 杂志文章

    doi:

    authors: Grannis GF,Lott JA

    更新日期:1978-04-01 00:00:00

  • Simultaneous determination of 6-thioguanine and methyl 6-mercaptopurine nucleotides of azathioprine in red blood cells by HPLC.

    abstract::6-thioguanine (6-TGN) and methyl 6-mercaptopurine nucleotides (Me6-MPNs) are the two major metabolites found in erythrocytes after administration of azathioprine. In an attempt to understand the role of these metabolites in the pharmacologic and toxic activity of thiopurines, we have developed a HPLC method for the si...

    journal_title:Clinical chemistry

    pub_type: 杂志文章

    doi:

    authors: Dervieux T,Boulieu R

    更新日期:1998-03-01 00:00:00

  • ISSAID/EMQN Best Practice Guidelines for the Genetic Diagnosis of Monogenic Autoinflammatory Diseases in the Next-Generation Sequencing Era.

    abstract:BACKGROUND:Monogenic autoinflammatory diseases are caused by pathogenic variants in genes that regulate innate immune responses, and are characterized by sterile systemic inflammatory episodes. Since symptoms can overlap within this rapidly expanding disease category, accurate genetic diagnosis is of the utmost importa...

    journal_title:Clinical chemistry

    pub_type: 杂志文章

    doi:10.1093/clinchem/hvaa024

    authors: Shinar Y,Ceccherini I,Rowczenio D,Aksentijevich I,Arostegui J,Ben-Chétrit E,Boursier G,Gattorno M,Hayrapetyan H,Ida H,Kanazawa N,Lachmann HJ,Mensa-Vilaro A,Nishikomori R,Oberkanins C,Obici L,Ohara O,Ozen S,Sarkisian T

    更新日期:2020-04-01 00:00:00

  • Flow-cytometric determination of high-density-lipoprotein binding sites on human leukocytes.

    abstract::In this method, leukocytes were isolated from 6 mL of EDTA-blood by density-gradient centrifugation and subsequently incubated with rhodamine isothiocyanate (RITC)-conjugated high-density lipoproteins (HDL). The receptor-bound conjugate particles were determined by fluorescent flow cytometry and compared with 125I-lab...

    journal_title:Clinical chemistry

    pub_type: 杂志文章

    doi:

    authors: Schmitz G,Wulf G,Brüning T,Assmann G

    更新日期:1987-12-01 00:00:00

  • Endothelial nitric oxide synthase gene polymorphisms and risk of coronary artery disease.

    abstract:BACKGROUND:Endothelial nitric oxide synthase (eNOS) could be a candidate gene for coronary artery disease (CAD). This study investigated the relationship of the eNOS Glu(298)-->Asp and T(786)-->C polymorphisms with the presence and severity of CAD in the Italian population. METHODS:We enrolled 415 unrelated individual...

    journal_title:Clinical chemistry

    pub_type: 杂志文章

    doi:10.1373/49.3.389

    authors: Colombo MG,Paradossi U,Andreassi MG,Botto N,Manfredi S,Masetti S,Biagini A,Clerico A

    更新日期:2003-03-01 00:00:00

  • Growth hormone (GH) receptor isoform in acromegaly: lower concentrations of GH but not insulin-like growth factor-1 in patients with a genomic deletion of exon 3 in the GH receptor gene.

    abstract:BACKGROUND:A genomic deletion of exon 3 (d3-GHR) of the growth hormone (GH) receptor (GHR) has been linked to the effectiveness of GH therapy in children with GH deficiency. Carriers of the d3-GHR genotype had higher GH-induced growth rates than children homozygous for the full-length (fl)-GHR. The aim of this study wa...

    journal_title:Clinical chemistry

    pub_type: 杂志文章

    doi:10.1373/clinchem.2007.085712

    authors: Schmid C,Krayenbuehl PA,Bernays RL,Zwimpfer C,Maly FE,Wiesli P

    更新日期:2007-08-01 00:00:00

  • Distinguishing Non-Small Cell Lung Cancer Subtypes in Fine Needle Aspiration Biopsies by Desorption Electrospray Ionization Mass Spectrometry Imaging.

    abstract:BACKGROUND:Distinguishing adenocarcinoma and squamous cell carcinoma subtypes of non-small cell lung cancers is critical to patient care. Preoperative minimally-invasive biopsy techniques, such as fine needle aspiration (FNA), are increasingly used for lung cancer diagnosis and subtyping. Yet, histologic distinction of...

    journal_title:Clinical chemistry

    pub_type: 杂志文章

    doi:10.1093/clinchem/hvaa207

    authors: Bensussan AV,Lin J,Guo C,Katz R,Krishnamurthy S,Cressman E,Eberlin LS

    更新日期:2020-11-01 00:00:00

  • Magnetizable solid-phase fluoroimmunoassay of thyroxine by a sequential addition technique.

    abstract::We describe a simple fluoroimmunoassay for the determination of thyroxine concentrations in serum. The method, "sequential addition, separation fluoroimmunoassay," involves both thyroxine labeled with fluorescein and magnetizable cellulose/iron oxide particles to which antibodies to thyroxine have been covalently link...

    journal_title:Clinical chemistry

    pub_type: 杂志文章

    doi:

    authors: Nargessi RD,Ackland J,Hassan M,Forrest GC,Smith DS,Landon J

    更新日期:1980-11-01 00:00:00

  • Detecting CFTR gene mutations by using primer oligo base extension and mass spectrometry.

    abstract::A new method for the reliable identification of localized variations in DNA by detection of associated diagnostic products with matrix-assisted laser desorption ionization time-of-flight mass spectrometry is described. The diagnostic products are generated by the primer oligo base extension (PROBE) reaction, which req...

    journal_title:Clinical chemistry

    pub_type: 杂志文章

    doi:

    authors: Braun A,Little DP,Köster H

    更新日期:1997-07-01 00:00:00

  • Simple method for the routine determination of betaine and N,N-dimethylglycine in blood and urine.

    abstract::A simple and convenient method using commercially available derivatization reagents is described for the measurement of betaine and N,N-dimethylglycine (DMG) in blood and urine. Precolumn derivatization of plasma or urine is performed directly in acetonitrile without extraction with p-bromophenacyl bromide and crown e...

    journal_title:Clinical chemistry

    pub_type: 杂志文章

    doi:

    authors: Laryea MD,Steinhagen F,Pawliczek S,Wendel U

    更新日期:1998-09-01 00:00:00

  • Identification of alpha(1)-antitrypsin variants in plasma with the use of proteomic technology.

    abstract:BACKGROUND:Proteomic technology permits the investigation of genetic metabolic diseases at the level of protein expression. Changes in the expression, polypeptide structure, and posttranslational modification of individual proteins can be detected in complex mixtures of proteins. METHODS:We used high-resolution two-di...

    journal_title:Clinical chemistry

    pub_type: 杂志文章

    doi:

    authors: Mills K,Mills PB,Clayton PT,Johnson AW,Whitehouse DB,Winchester BG

    更新日期:2001-11-01 00:00:00

  • Stabilized analysis of antidepressant drugs by solvent-recycled liquid chromatography: procedure and proposed resolution mechanisms for chromatography.

    abstract::In this efficient extraction and isocratic liquid-chromatographic procedure for measuring eight antidepressant drugs in serum (amoxapine, 8-hydroxyamoxapine, doxepin, desmethyldoxepin, imipramine, desipramine, amitriptyline, and nortriptyline), they are extracted from serum as a group by use of disposable solid-phase ...

    journal_title:Clinical chemistry

    pub_type: 杂志文章

    doi:

    authors: Lensmeyer GL,Evenson MA

    更新日期:1984-11-01 00:00:00

  • Use of precision profiles to evaluate precision of the automated leukocyte differential.

    abstract::The commonly used methods of assessing the precision of the automated leukocyte differential have certain drawbacks that affect the validity and comparability of results. In the present report, we introduce a procedure based on building precision profiles from a large number of within-run imprecision experiments. The ...

    journal_title:Clinical chemistry

    pub_type: 杂志文章

    doi:

    authors: Hübl W,Tlustos L,Bayer PM

    更新日期:1996-07-01 00:00:00

  • Inhibitory effects of micronized fenofibrate on carotid atherosclerosis in patients with essential hypertension.

    abstract:BACKGROUND:The coexistence of hypertension and dyslipidemia synergistically increases the risk of cardiovascular events. We investigated the effect of the lipid-lowering agent micronized fenofibrate on inhibition of carotid atherosclerosis in patients with essential hypertension and mild hyperlipidemia. METHODS:We mea...

    journal_title:Clinical chemistry

    pub_type: 杂志文章,随机对照试验

    doi:10.1373/clinchem.2006.074724

    authors: Zhu S,Su G,Meng QH

    更新日期:2006-11-01 00:00:00

  • Determination of shelf life and activation energy for tumor necrosis factor-alpha in cerebrospinal fluid samples.

    abstract::The cytokine rumor necrosis factor-alpha (TNF-alpha) plays a prominent role in the inflammatory response and has been quantified in several kinds of body fluids. A safe method for keeping samples under the best storage conditions for later studies is clearly needed. We estimated TNF-alpha shelf life by using an accele...

    journal_title:Clinical chemistry

    pub_type: 杂志文章

    doi:

    authors: Cruz Ruiz M,Recio Quijano F,López Cortés LF,Hebles Duvison M,Vázquez Rubio R

    更新日期:1996-05-01 00:00:00

  • A simple, direct radioimmunoassay for plasma cortisol, featuring a 125I radioligand and a solid-phase separation technique.

    abstract::A simple, direct radioimmunoassay for cortisol in human serum and plasma is described. An antiserum, raised in sheep to a cortisol-3-(O-carboxymethyl)oxime/bovine serum albumin conjugate, is coupled to microcellulose. No extraction is required because plasma samples and standards are incubated with the antiserum and a...

    journal_title:Clinical chemistry

    pub_type: 杂志文章

    doi:

    authors: Riad-Fahmy D,Read GF,Gaskell SJ,Dyas J,Hindawi R

    更新日期:1979-05-01 00:00:00

  • Method for testing antiserum titer and avidity in nephelometric systems.

    abstract::Antiserum performance in a nephelometric system can be characterized by parameters derived from measuring reaction rates. The characterization process is derived from a series of dose-response curves (elicited nephelometric response vs antigen concentration) generated from various dilutions of the antiserum being test...

    journal_title:Clinical chemistry

    pub_type: 杂志文章

    doi:

    authors: Hudson GA,Ritchie RF,Haddow JE

    更新日期:1981-11-01 00:00:00

  • Use of natriuretic peptides to guide and monitor heart failure therapy.

    abstract:BACKGROUND:Plasma B-type cardiac natriuretic peptides reflect cardiac structure and function and have proven roles in assisting in the diagnosis of acute heart failure. They are independent prognostic indicators across the full spectrum of cardiovascular disease. Serial changes in plasma B-type cardiac natriuretic pept...

    journal_title:Clinical chemistry

    pub_type: 杂志文章,评审

    doi:10.1373/clinchem.2011.165704

    authors: Richards AM,Troughton RW

    更新日期:2012-01-01 00:00:00

  • External quality assurance program for PCR amplification of genomic DNA: an Italian experience.

    abstract:BACKGROUND:External quality assurance (EQA) programs for diagnostic tests based on nucleic acid amplification have not been widely implemented in clinical laboratories and remain limited to few tests. Development of specific EQA programs based on application-based proficiency testing for any diagnostic molecular target...

    journal_title:Clinical chemistry

    pub_type: 杂志文章

    doi:10.1373/49.5.782

    authors: Raggi CC,Pinzani P,Paradiso A,Pazzagli M,Orlando C

    更新日期:2003-05-01 00:00:00

  • Combi scheme: new combined internal/external quality-assessment scheme in The Netherlands.

    abstract::The Dutch Foundation for Quality Assessment in Clinical Chemistry (SKZL) is the professional organization that conducts external quality-assessment schemes in The Netherlands. However, such schemes in fact assess the performance of the internal quality-control systems of the participating laboratories. In this paper w...

    journal_title:Clinical chemistry

    pub_type: 杂志文章

    doi:

    authors: Steigstra H,Jansen RT,Baadenhuijsen H

    更新日期:1991-07-01 00:00:00

  • Role of biomarkers in identifying and understanding environmentally induced disease.

    abstract::Establishing associations between environmental agents and disease presents challenges to both epidemiologists and toxicologists, particularly in cases of complex gene-environment interactions and when there is a long latency between exposure and disease. Biologic markers, physiological signals that reflect exposure, ...

    journal_title:Clinical chemistry

    pub_type: 杂志文章,评审

    doi:

    authors: Silbergeld EK,Davis DL

    更新日期:1994-07-01 00:00:00

  • Assessment of urea and other uremic markers for quantification of dialysis efficacy.

    abstract::To validate azotemic markers as an index for intradialytic changes in solute concentration, we compared eight solutes (pseudouridine, xanthine, hypoxanthine, peak 4, peak 5, p-hydroxyhippuric acid, indoxyl sulfate, and hippuric acid) with five classical azotemic markers (urea, creatinine, uric acid, phosphate, and pot...

    journal_title:Clinical chemistry

    pub_type: 杂志文章

    doi:

    authors: Vanholder RC,De Smet RV,Ringoir SM

    更新日期:1992-08-01 00:00:00

  • Automated enzymic measurement of total cholesterol in serum.

    abstract::We describe a completely automated enzymic system for measuring total cholesterol in serum. All reagents are contained in an analytical test pack and the test is performed on Du Pont's Automatic Clinical Analyzer (aca), which mixes the sample (20 microliter) and reagents and performs the necessary absorbance measureme...

    journal_title:Clinical chemistry

    pub_type: 杂志文章

    doi:

    authors: Rautela GS,Liedtke RJ

    更新日期:1978-01-01 00:00:00

  • Challenges in the diagnosis of the antiphospholipid syndrome.

    abstract:BACKGROUND:The antiphospholipid syndrome (APS) is an important cause of acquired thromboembolic complications and pregnancy morbidity. Its diagnosis is based on clinical and laboratory criteria, defined by strict guidelines. The original clinical and laboratory criteria for the identification of APS patients were publi...

    journal_title:Clinical chemistry

    pub_type: 杂志文章,评审

    doi:10.1373/clinchem.2009.133678

    authors: Devreese K,Hoylaerts MF

    更新日期:2010-06-01 00:00:00

  • Specific micro-radioimmunoassay for prednisolone in serum.

    abstract::Use of an 125I radioimmunoassay involving antiserum coupled to magnetizable cellulose, after prednisolone-21-phosphate interference is removed by dichloromethane extraction at pH 7.4 and endogenous glucocorticoid interference is removed by selective chemical derivatization with Girard's Reagent T [(carboxymethyl)trime...

    journal_title:Clinical chemistry

    pub_type: 杂志文章

    doi:

    authors: Olivesi A,Smith DS,White GW,Pourfarzaneh M

    更新日期:1983-07-01 00:00:00