Mg2+- or Mn2+-dependent endonuclease activities of human myeloid leukemia cells capable of producing nucleosomal-size DNA fragmentation.

Abstract:

:The presence of at least two distinct Mg2+- or Mn2+-dependent, Ca2+-independent endonuclease activities was shown in the myeloid leukemia cell line P39. One of them was recovered from nuclear extracts and the other from a cytoplasmic fraction. The molecular size of the former was 30 kDa in both gel filtration and activity gel and that of the latter approximately 130-140 kDa in gel filtration and 65-70 kDa in activity gel. These two activities were almost completely inhibited by 0.1 mM ZnCl2 or 0.1 mM aurintricarboxylic acid, common inhibitors of apoptosis. Both could produce nucleosomal-size DNA fragmentation when incubated with diethyl-pyrocarbonate-treated nuclei as substrates, and the pattern of cleavage was 3'-OH and 5'-P. Taken together, either or both of these activities may be associated with apoptosis of myeloid leukemia cells.

authors

Kawabata H,Anzai N,Masutani H,Hirama T,Hishita T,Dodo M,Masuda T,Yoshida Y,Okuma M

doi

10.1006/bbrc.1997.6362

subject

Has Abstract

pub_date

1997-04-07 00:00:00

pages

133-8

issue

1

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(97)96362-2

journal_volume

233

pub_type

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