Abstract：
OBJECTIVE:To determine the frequency of mutation and overexpression of the p53 tumor suppressor gene in human soft tissue sarcomas. DESIGN:A total of 31 soft tissue sarcomas were analyzed by immunohistochemistry for the expression of p53 protein and were subsequently investigated by the polymerase chain reaction technique and direct sequence analysis of exons 5 through 8 in the p53 gene. SETTING:The specimens were collected over a 3-year period in the laboratories at our large teaching hospital in Seoul, Republic of Korea. PATIENTS:Thirty-one patients with soft tissue tumor were surgically treated and diagnosed as having either malignant fibrous histiocytoma, rhabdomyosarcoma, or leiomyosarcoma. RESULTS:Overexpression of p53 was seen in 17 (55%) of 31 sarcomas, including 9 (64%) of 14 malignant fibrous histiocytomas, 4 (44%) of 9 rhabdomyosarcomas, and 4 (50%) of 8 leiomyosarcomas. Seven cases (23%) demonstrated mutations in the p53 gene. Six had a single mutation, whereas one showed triple mutations. There were seven mutations in exon 5, one in exon 6, and one in exon 7. All of the mutations were missense mutations, resulting in changes in the predicted amino acid sequence. Among the nine mutations, seven (78%) were transversions and two (22%) were transitions. CONCLUSIONS:Mutation of the p53 tumor suppressor gene, with resultant overexpression of p53 protein, frequently occurs in human soft tissue sarcomas, supporting the role of p53 mutations in the pathogenesis of soft tissue sarcoma and the possible usefulness of p53 immunolocalization as a screening method for p53 mutations.
journal_name
Arch Pathol Lab Medjournal_title
Archives of pathology & laboratory medicineauthors
Yoo J,Lee HK,Kang CS,Park WS,Lee JY,Shim SIsubject
Has Abstractpub_date
1997-04-01 00:00:00pages
395-9issue
4eissn
0003-9985issn
1543-2165journal_volume
121pub_type
杂志文章abstract：CONTEXT:Implementation of proficiency testing for gynecologic cytology was delayed 20 years because of challenges addressing the subjective nature of cytologic interpretation and replicating normal working conditions. Concern remains regarding test scoring, slide validation, test environment, and other issues. How thes...
journal_title：Archives of pathology & laboratory medicine
pub_type： 共识发展会议,杂志文章
doi：10.5858/arpa.2012-0094-OA
更新日期：2013-02-01 00:00:00
abstract：CONTEXT:- Current 2013 World Health Organization classification of tumors of soft tissue arranges these tumors into 12 groups according to their histogenesis. Tumor behavior is classified as benign, intermediate (locally aggressive), intermediate (rarely metastasizing), and malignant. In our practice, a general approac...
journal_title：Archives of pathology & laboratory medicine
pub_type： 杂志文章,评审
doi：10.5858/arpa.2016-0417-RA
更新日期：2017-08-01 00:00:00
abstract：CONTEXT:The uses of monoclonal antibodies via immunochemistry have been reported frequently within the literature using various methodologies with applications to cytology specimens. The direct application of immunochemistry to cytology may have a variety of pitfalls that the general pathologist familiar with its appli...
journal_title：Archives of pathology & laboratory medicine
pub_type： 杂志文章,评审
doi：10.1043/1543-2165(2008)132[373:AOITC]2.0.CO;2
更新日期：2008-03-01 00:00:00
abstract：:Calbindin-D28k is a highly conserved 28,000 (dalton) molecular-weight (Mr) calcium binding protein with broad tissue distribution, yet cell-type-specific expression predominantly in subpopulations of central and peripheral nervous system neurons, distal tubular cells of the kidney, and enteric neuroendocrine cells. A ...
journal_title：Archives of pathology & laboratory medicine
pub_type： 杂志文章
doi：
更新日期：1994-06-01 00:00:00
abstract：:Critical analysis of the evidence supporting the use of prognostic markers is needed for these to be used most appropriately in patient management. The College of American Pathologists recently sponsored a national conference to review the status of tumor markers for carcinomas of the breast, colon, and prostate gland...
journal_title：Archives of pathology & laboratory medicine
pub_type： 共识发展会议,杂志文章,评审
doi：
更新日期：1995-12-01 00:00:00
abstract：CONTEXT:Although the healthy esophageal mucosa contains no eosinophils, eosinophilic infiltration is observed in 2 major clinicopathologic settings: gastroesophageal reflux disease (GERD) and eosinophilic esophagitis (EE). The prevalence of EE is increasing in many countries, and this increase seems only partly to be d...
journal_title：Archives of pathology & laboratory medicine
pub_type： 杂志文章,评审
doi：10.1043/1543-2165-134.6.815
更新日期：2010-06-01 00:00:00
abstract：:Primary germinal tumors of the mediastinum are very rare. We studied a 67-year-old man with a primary embryonal carcinoma and choriocarcinoma of the mediastinum. To our knowledge, this is the oldest patient described as having this lesion. The diagnosis of primary germinal tumor of the mediastinum should not be made u...
journal_title：Archives of pathology & laboratory medicine
pub_type： 杂志文章
doi：
更新日期：1982-10-01 00:00:00
abstract：:Critical values in anatomic pathology are usually information sensitive, whereas most such values in laboratory medicine are time sensitive. However, there is an important time element in anatomic pathology as well. Pathologists should be aware that many medicolegal actions against radiologists are based on failure to...
journal_title：Archives of pathology & laboratory medicine
pub_type： 杂志文章
doi：10.1043/1543-2165(2006)130[641:CCVIAP]2.0.CO;2
更新日期：2006-05-01 00:00:00
abstract：:kappa Light chain deposits occurring in myocardium as a complication of multiple myeloma were identified ultrastructurally and immunohistochemically in a right ventricular endomyocardial biopsy specimen from a patient who presented with clinical and hemodynamic findings of restrictive cardiomyopathy. These deposits we...
journal_title：Archives of pathology & laboratory medicine
pub_type： 杂志文章
doi：
更新日期：1988-11-01 00:00:00
abstract：:Intracellular inclusions in lymphoproliferative disorders are not common. Multiple different types of inclusions have been reported in chronic lymphocytic leukemia (CLL), including vacuoles, crystals, and pseudocrystals. Most of the reported cases were seen in the bone marrow lymphocytes, and the majority of these on ...
journal_title：Archives of pathology & laboratory medicine
pub_type： 杂志文章
doi：10.1043/0003-9985(2003)127<0618:IFIITP>2.0.CO;2
更新日期：2003-05-01 00:00:00
abstract：CONTEXT:Inflicted traumatic brain injury of infants and young children results in a complex array of autopsy findings. In many cases, immunostains for beta-amyloid precursor protein are used to detect axonal injury. Interpretation of the gross, microscopic, and immunostaining results requires the integration of the man...
journal_title：Archives of pathology & laboratory medicine
pub_type： 杂志文章,评审
doi：10.1043/1543-2165(2006)130[712:AOOIHI]2.0.CO;2
更新日期：2006-05-01 00:00:00
abstract：:Herein is presented a detailed description of the materials and methods employed in the conduct of an ongoing system for the independent evaluation (or testing) of individual sophomore medical students, as part of a major sophomore course in anatomic pathology. The academic environment and the advantages and disadvant...
journal_title：Archives of pathology & laboratory medicine
pub_type： 杂志文章
doi：
更新日期：1986-11-01 00:00:00
abstract：OBJECTIVE:To determine the diagnostic accuracy of an investigational test for human immunodeficiency virus (HIV) envelope antibodies in urine. DESIGN:Matched blood and urine specimens were tested for HIV by two independent laboratories, both of which were blinded to all results at the other site. Duplicate positive en...
journal_title：Archives of pathology & laboratory medicine
pub_type： 杂志文章
doi：
更新日期：1995-02-01 00:00:00
abstract：:Hemangiopericytoma of soft tissue is a controversial pathologic entity. The relative nonspecificity of the characteristic branching capillary pattern and cytologic features of the constituent cells, in addition to the lack of a distinct immunohistochemical staining profile, has resulted in uncertainty and a lack of co...
journal_title：Archives of pathology & laboratory medicine
pub_type： 杂志文章
doi：10.1043/0003-9985(1999)123<0941:LH>2.0.CO;2
更新日期：1999-10-01 00:00:00
abstract：:Previous studies have reported on the pathologic spectrum of perinatal mortality; however, in our opinion, the problems pertaining to the term newborn have not been emphasized sufficiently. We reviewed the autopsies of all term infants up to 2 months of age in a ten-year period (July 1975 to July 1985). These 342 pati...
journal_title：Archives of pathology & laboratory medicine
pub_type： 杂志文章
doi：
更新日期：1988-02-01 00:00:00
abstract：:Two autopsy cases of sympodial monsters, sympus dipus, were studied. They are of interest not only because they show the characteristic malformations of the limbs and the viscera below the diaphragm, but because one case also shows Potter's face and an atrial septal defect of the heart. In addition, histologic determi...
journal_title：Archives of pathology & laboratory medicine
pub_type： 杂志文章
doi：
更新日期：1981-10-01 00:00:00
abstract：:A case of alveolar soft-part sarcoma was studied. It illustrates the use of touch imprint preparations on fresh tissue sections examined while frozen. The technique is simple to use and can be applied to a wide variety of neoplastic and inflammatory lesions. Touch imprints substantially contribute to accurate histopat...
journal_title：Archives of pathology & laboratory medicine
pub_type： 杂志文章
doi：
更新日期：1981-05-01 00:00:00
abstract：:Ten cases of gynecomastia were studied by electron microscopy. The ducts showed proliferation of both epithelial and myoepithelial cells. Intracytoplasmic lumina, previously thought to be a feature of malignant breast lesions, were seen. Squamous metaplasia was observed in some cases. The stroma showed fibroblasts, my...
journal_title：Archives of pathology & laboratory medicine
pub_type： 杂志文章
doi：
更新日期：1979-11-01 00:00:00
abstract：:Idiopathic nephrotic syndrome occurred in a patient with sarcoidosis. Typical features of focal segmental glomerulosclerosis were present on kidney biopsy. A unique finding was the occurrence of IgA in blood vessels of the skin and lymph node. Whereas the sarcoid hilar adenopathy responded to steroid therapy, the neph...
journal_title：Archives of pathology & laboratory medicine
pub_type： 杂志文章
doi：
更新日期：1978-11-01 00:00:00
abstract：:Inherited mutations in the genes BRCA1 and BRCA2 are associated with a significantly increased risk of breast cancer, particularly before the age of 50 years, as well as an increased risk of ovarian cancer. Patients with early-onset breast cancer or ovarian cancer at any age with a family history of either disease are...
journal_title：Archives of pathology & laboratory medicine
pub_type： 杂志文章,评审
doi：10.1043/0003-9985(1999)123<1023:LDOHST>2.0.CO;2
更新日期：1999-11-01 00:00:00
abstract：CONTEXT:Neuroendocrine lung neoplasms are a heterogeneous group of tumors with different clinical behavior and prognosis. OBJECTIVES:To evaluate the expression of p53, KLF4, and p21 in neuroendocrine lung neoplasms and to analyze the influence that expression has on the prognosis of those tumors. DESIGN:All neuroendo...
journal_title：Archives of pathology & laboratory medicine
pub_type： 杂志文章
doi：10.5858/arpa.2013-0119-OA
更新日期：2014-07-01 00:00:00
abstract：:Keratin and carcinoembryonic antigen immunoperoxidase staining were applied to lung cancer cells in paraffin sections of cell blocks from sputa and bronchial washings. The results correlate well with cytologic diagnosis. The large cell carcinomas are nonreactive with both antibodies. ...
journal_title：Archives of pathology & laboratory medicine
pub_type： 杂志文章
doi：
更新日期：1989-01-01 00:00:00
abstract：CONTEXT:-Whole-slide imaging technology offers promise for rapid, Internet-based telepathology consultations between institutions. Before implementation, technical issues, pathologist adaptability, and morphologic pitfalls must be well characterized. OBJECTIVE:-To determine whether interpretation of whole-slide images...
journal_title：Archives of pathology & laboratory medicine
pub_type： 杂志文章
doi：10.1043/1543-2165-133.12.1949
更新日期：2009-12-01 00:00:00
abstract：CONTEXT:Mutation status of the KRAS gene identifies a distinct disease subtype of metastatic colorectal carcinoma that does not respond to antibody therapeutics targeting the epidermal growth factor receptor. This is currently the only validated marker in metastatic colorectal carcinoma with a clear implication in trea...
journal_title：Archives of pathology & laboratory medicine
pub_type： 杂志文章,评审
doi：10.5858/arpa.2011-0478-RA
更新日期：2012-10-01 00:00:00
abstract：:We studied 16 cases of bile duct adenomas, lesions that are distinct from Meyenburg complex. The former lesion is made up of small tubular structures with little or no lumen, is usually single and subcapsular, and is unrelated to cystic disease. It must be differentiated from early carcinoma. Of the six cases encounte...
journal_title：Archives of pathology & laboratory medicine
pub_type： 杂志文章
doi：
更新日期：1984-11-01 00:00:00
abstract：:A rare angiomyxoma of umbilica cord occurred in a male infant. The presence of such a tumor with its vascular component arising from a hypoplastic umbilical artery supports the hypothesis that the lesions arose through improper placental vascularization. ...
journal_title：Archives of pathology & laboratory medicine
pub_type： 杂志文章
doi：
更新日期：1978-04-01 00:00:00
abstract：:Knowledge of Toxoplasma seroprevalence among human immunodeficiency virus-infected patients identifies candidates for potential prophylaxis and may influence management among patients who present with mass lesions of the central nervous system. Toxoplasma antibody seroprevalence among persons with human immunodeficien...
journal_title：Archives of pathology & laboratory medicine
pub_type： 杂志文章
doi：
更新日期：1994-08-01 00:00:00
abstract：:We report a case of fatal fibrosing peritonitis in a 28-year-old drug abuser. The extensive fibrosis, which was limited to the abdominal cavity, contained birefringent material. Silica was identified by spectrophotometric analysis. Silica is a component of talc (magnesium silicate) used as a drug filler. Relatively sm...
journal_title：Archives of pathology & laboratory medicine
pub_type： 杂志文章
doi：
更新日期：1985-08-01 00:00:00
abstract：OBJECTIVE:To summarize current knowledge and recent progress pertaining to platelet concentrate preparations, modifications, and future prospects for platelet substitutes. METHODS:Current publications identified through a search of an electronic literature database were evaluated and reviewed. Relevant data were abstr...
journal_title：Archives of pathology & laboratory medicine
pub_type： 杂志文章,评审
doi：10.1043/0003-9985(1999)123<0889:P>2.0.CO;2
更新日期：1999-10-01 00:00:00
abstract：:The pathologic correlate of the clinical terms subendocardial and transmural applied to myocardial infarcts is uncertain. To examine this question, we reviewed the morphology of 204 hearts with single myocardial infarcts studied at autopsy after coronary arteriography and fixation in distention. The thickness of survi...
journal_title：Archives of pathology & laboratory medicine
pub_type： 杂志文章
doi：
更新日期：1986-05-01 00:00:00
