当前位置： SCI文献检索 > ACTA OBSTETRICIA ET GYNECOLOGICA SCANDINAVICA期刊下所有文献 > Ability of intrauterine bacterial lipopolysaccharide to cause in situ uterine contractions in pregnant rabbits.

Ability of intrauterine bacterial lipopolysaccharide to cause in situ uterine contractions in pregnant rabbits.

Abstract：

BACKGROUND:To investigate the ability of bacterial lipopolysaccharide delivered by the intra-uterine route to cause uterine contractions in rabbits, and to assess the suppressive effect of urinary trypsin inhibitor on them. METHODS:Both pregnant and non-pregnant rabbits were chronically implanted with a force-transducer to make it possible to record isometric uterine contractions under unanesthetized and unrestrained conditions. Lipopolysaccharide (10 micrograms/animal) was administered via a catheter to their uteri; and then, after confirmation of lipopolysaccharide-induced uterine contractions, urinary trypsin inhibitor (3,000 or 10,000 units/animal/time) or saline solution was injected through the catheter, 5 times for pregnant animals or 3 times for non-pregnant animals at 1-hour intervals in both cases. Their uterine contractions were continuously recorded for 3 to 5 hours. Effects of lipopolysaccharide (10 micrograms/ml) and urinary trypsin inhibitor (100 and 1,000 units/ml) on the contraction of isolated uteri from pregnant mice were also measured, as was their production of prostaglandin E2 and prostaglandin F2 alpha by an enzyme immunoassay method. RESULTS:Lipopolysaccharide augmented the in situ uterine contractions in both pregnant and non-pregnant rabbits, as well as the in vitro contractions of isolated uteri from pregnant mice. Lipopolysaccharide also increased the uterine prostaglandin production. Urinary trypsin inhibitor inhibited significantly the lipopolysaccharide-induced uterine contractions and the prostaglandin production. CONCLUSIONS:Lipopolysaccharide enhanced uterine contractions through, at least partly, a direct mechanism via uterine prostaglandin production, which action could explain the onset of preterm delivery due to intrauterine bacterial infection. As urinary trypsin inhibitor suppressed the lipopolysaccharide-induced uterine contractions, this inhibitor may be a hopeful candidate of a drug for prevention of preterm delivery.

journal_name

Acta Obstet Gynecol Scand

journal_title

Acta obstetricia et gynecologica Scandinavica

authors

Katsuki Y,Kaga N,Kakinuma C,Takagaki K,Kajikawa S,Shibutani Y

doi

10.3109/00016349709047780

subject

Has Abstract

pub_date

1997-01-01 00:00:00

pages

26-32

issue

eissn

0001-6349

issn

1600-0412

journal_volume

pub_type

杂志文章

在线工具

Ability of intrauterine bacterial lipopolysaccharide to cause in situ uterine contractions in pregnant rabbits.