Anti-CD10 immunoperoxidase staining of paraffin-embedded acute leukemias: comparison with flow cytometric immunophenotyping.

Abstract:

:CD10 is common in B-precursor acute lymphoblastic leukemia (ALL) but is rare in acute myeloid leukemia (AML). However, until recently, analysis for CD10 has generally required fresh or frozen tissue. 56C6 is a monoclonal antibody that is now commercially available for the detection of CD10 in routinely processed paraffin-embedded tissue. Immunoperoxidase stains for CD10 on paraffin-embedded bone marrow core biopsy specimens (B5-fixed, decalcified) and marrow aspirate clots (formalin-fixed) were compared with flow cytometric immunophenotyping for CD10 on fresh cell suspensions in 20 cases of AML and in 30 cases of ALL. CD10 detection by immunohistochemistry agreed with CD10 by flow cytometry in 98% (49 of 50) of acute leukemias. The results matched in 100% (20 of 20) of AML. Five percent (1 of 20) of AMLs expressed CD10. Two of the AMLs with monocytoid differentiation were interpreted as negative for CD10 by flow cytometry, although these had nonspecific dim immunofluorescence for multiple markers, including CD10, and these cases were negative by immunohistochemistry. CD10 detection by immunohistochemistry agreed with CD10 by flow cytometry in 97% (29 of 30) of ALL. Eighty-four percent (21 of 25) of B-precursor ALL and 40% (2/5) of T-lineage ALL expressed CD10 by immunohistochemistry. In 1 case of B-precursor ALL, CD10 was dimly positive in 24% of the blasts by flow cytometry but negative by immunohistochemistry. We conclude that immunohistochemical staining of paraffin-embedded tissue, either B5- or formalin-fixed, is an effective method for the detection of CD10 in acute leukemia. This technique is useful in distinguishing AML from ALL.

journal_name

Hum Pathol

journal_title

Human pathology

authors

Bavikatty NR,Ross CW,Finn WG,Schnitzer B,Singleton TP

doi

10.1053/hupa.2000.6276

subject

Has Abstract

pub_date

2000-09-01 00:00:00

pages

1051-4

issue

9

eissn

0046-8177

issn

1532-8392

pii

S0046-8177(00)89760-9

journal_volume

31

pub_type

杂志文章
  • Exploration of BRAFV600E as a diagnostic adjuvant in the non-invasive follicular thyroid neoplasm with papillary-like nuclear features (NIFTP).

    abstract::The non-invasive follicular thyroid neoplasm with papillary-like nuclear features (NIFTP) and encapsulated follicular variant of papillary thyroid carcinoma (EFVPTC) are distinguished from classical papillary thyroid carcinoma with predominantly follicular architecture (CPTCPFA) by distinct histomorphologic and molecu...

    journal_title:Human pathology

    pub_type: 杂志文章

    doi:10.1016/j.humpath.2018.06.033

    authors: Johnson DN,Sadow PM

    更新日期:2018-12-01 00:00:00

  • HMB-45, S-100, NK1/C3, and MART-1 in metastatic melanoma.

    abstract::The diagnosis of melanoma metastatic to lymph node remains a difficult problem given its histological diversity. We examined the staining patterns of S-100, NK1/C3, HMB-45, and MART-1 (DC10) in melanoma metastases to lymph nodes. Immunohistochemical stains were performed on tissue sections of 126 formalin-fixed lymph ...

    journal_title:Human pathology

    pub_type: 杂志文章

    doi:10.1016/j.humpath.2003.09.019

    authors: Zubovits J,Buzney E,Yu L,Duncan LM

    更新日期:2004-02-01 00:00:00

  • NF2 and ATRX gene copy number losses on a case of ovarian ependymoma.

    abstract::Ovarian ependymomas are rare glial neoplasms that typically occur in women on their third to fourth decades of life. They are histologically similar to ependymomas of the central nervous system but may have a broader immunophenotype. We describe a 27-year-old woman who presented to the emergency department with a 3-we...

    journal_title:Human pathology

    pub_type: 杂志文章

    doi:10.1016/j.humpath.2018.06.019

    authors: Lopez LV,Bhattacharyya S,Carlsen ED,Bartlett D,Rao UNM

    更新日期:2019-01-01 00:00:00

  • Profiles of chemokine receptors in melanocytic lesions: de novo expression of CXCR6 in melanoma.

    abstract::Selective expression of certain chemokine receptors by melanoma cells and the presence of their ligands in tissues might govern organ site-specific metastasis. Because the expression profile of chemokine receptors in tissues of melanocytic origin is unknown, we performed a comprehensive study on melanocytic tissue sam...

    journal_title:Human pathology

    pub_type: 杂志文章

    doi:10.1016/j.humpath.2006.11.013

    authors: Seidl H,Richtig E,Tilz H,Stefan M,Schmidbauer U,Asslaber M,Zatloukal K,Herlyn M,Schaider H

    更新日期:2007-05-01 00:00:00

  • Extratubal secondary trophoblastic implants: clinicopathologic correlation and review of the literature.

    abstract::Extratubal secondary trophoblastic implants (ESTI) are a rare complication of conservative laparoscopic procedures for tubal ectopic pregnancies. These implants present with persistent beta-hCG titers postoperatively and are probably the result of disruption of the ectopic pregnancy at salpingostomy or morcellation of...

    journal_title:Human pathology

    pub_type: 杂志文章

    doi:10.1016/s0046-8177(98)90231-3

    authors: Doss BJ,Jacques SM,Qureshi F,Ramirez NC,Lawrence WD

    更新日期:1998-02-01 00:00:00

  • Simultaneous visualization of Helicobacter pylori and gastric morphology: a new stain.

    abstract::Although the histopathologic patterns associated with Helicobacter pylori infection have been described, details of the interaction between bacteria, epithelial cells, and inflammatory cells remain elusive. One of the limiting factors has been the lack of a staining technique that allows the simultaneous visualization...

    journal_title:Human pathology

    pub_type: 杂志文章

    doi:10.1016/0046-8177(94)90191-0

    authors: Genta RM,Robason GO,Graham DY

    更新日期:1994-03-01 00:00:00

  • Oncocytic variant of malignant gastrointestinal neuroectodermal tumor: a potential diagnostic pitfall.

    abstract::Malignant gastrointestinal neuroectodermal tumor (MGNET) is a very rare, aggressive malignant neoplasm that may occur in any location in the gastrointestinal tract. Malignant gastrointestinal neuroectodermal tumors typically consist of sheet-like to pseudopapillary proliferation of primitive-appearing epithelioid cell...

    journal_title:Human pathology

    pub_type: 杂志文章

    doi:10.1016/j.humpath.2016.05.026

    authors: Boland JM,Folpe AL

    更新日期:2016-11-01 00:00:00

  • Regulation of proliferation/apoptosis equilibrium by mitogen-activated protein kinases in normal, hyperplastic, and carcinomatous human prostate.

    abstract::This study investigate the expression of the mitogen-activated protein kinases (MAPKs) in normal prostate, benign prostatic hyperplasia (BPH), and prostatic cancer (PC), and also the possible relationship between the activity of these MAPKs and the apoptosis/proliferation index. Immunochemical techniques were carried ...

    journal_title:Human pathology

    pub_type: 杂志文章

    doi:10.1053/hupa.2002.32227

    authors: Royuela M,Arenas MI,Bethencourt FR,Sánchez-Chapado M,Fraile B,Paniagua R

    更新日期:2002-03-01 00:00:00

  • Viral genotypes and p53 expression in Epstein-Barr virus-associated primary malignant lymphomas of the intestines.

    abstract::A small number (4% to 6%) of primary malignant lymphomas arising in the intestines express the EBV genome. However, in these tumors, the viral genotype and the role of tumor suppressor gene p53 have not been investigated. We sought to determine what genotype of EBV is frequently involved and whether the expression of ...

    journal_title:Human pathology

    pub_type: 杂志文章

    doi:10.1016/s0046-8177(99)90030-8

    authors: Kim YS,Kim JB,Kang YK,Nam ES,Park SH,Kim I

    更新日期:1999-10-01 00:00:00

  • Expression of classic cadherins type I in urothelial neoplastic progression.

    abstract::Loss or reduced expression of E-cadherin has been shown to be associated with poor survival in patients with bladder cancer. In numerous cases, loss of E-cadherin expression in bladder tumors has been accompanied by continued association of catenins with the membrane, suggestive of the expression of an alternative cad...

    journal_title:Human pathology

    pub_type: 杂志文章

    doi:10.1053/hupa.2001.21140

    authors: Rieger-Christ KM,Cain JW,Braasch JW,Dugan JM,Silverman ML,Bouyounes B,Libertino JA,Summerhayes IC

    更新日期:2001-01-01 00:00:00

  • Expression and prognostic significance of matrix metalloproteinases and their tissue inhibitors in primary neuroendocrine carcinoma of the skin.

    abstract::Matrix metalloproteinases (MMPs) and their tissue inhibitors (TIMPs) have been implicated in the development and progression of many tumors, but data for primary neuroendocrine carcinoma (PNC) of the skin are lacking. The aim of the study was to assess the expression of MMPs and TIMPs in PNC and to evaluate their prog...

    journal_title:Human pathology

    pub_type: 杂志文章

    doi:10.1053/hupa.2003.51

    authors: Massi D,Franchi A,Ketabchi S,Paglierani M,Pimpinelli N,Santucci M

    更新日期:2003-01-01 00:00:00

  • T-cell subsets in sarcoidosis: an immunocytochemical investigation of blood, bronchoalveolar lavage fluid, and prescalenic lymph nodes from eight patients.

    abstract::T-lymphocyte subsets in peripheral blood, bronchoalveolar lavage fluid, and prescalenic lymph nodes from eight patients with sarcoidosis were evaluated with monoclonal antibodies. Both in lung and lymph nodes significant increases in helper T cells were demonstrated, except in patients with stage III pulmonary disease...

    journal_title:Human pathology

    pub_type: 杂志文章

    doi:10.1016/s0046-8177(86)80037-5

    authors: Viale G,Codecasa L,Bulgheroni P,Giobbi A,Madonini E,Dell'Orto P,Coggi G

    更新日期:1986-05-01 00:00:00

  • Primary pulmonary hyalinizing clear cell carcinoma of bronchial submucosal gland origin.

    abstract::Hyalinizing clear cell carcinoma (HCCC) has only been described in salivary glands of the head and neck. We report a 38-year-old man with a 2.6-cm lung tumor that was growing in a peribronchial location and had morphologic features of HCCC. The tumor cells expressed cytokeratin 7 and keratin AE1/AE3, and the vast majo...

    journal_title:Human pathology

    pub_type: 杂志文章

    doi:10.1016/j.humpath.2014.11.010

    authors: García JJ,Jin L,Jackson SB,Larsen BT,Lewis JE,Sukov WR,Roden AC

    更新日期:2015-03-01 00:00:00

  • A rosette-forming glioneuronal tumor of the spinal cord: the first case of a rosette-forming glioneuronal tumor originating from the spinal cord.

    abstract::Rosette-forming glioneuronal tumors of the fourth ventricle are rare brain tumors, and only 19 such lesions have been previously reported. This report presents the first case of a rosette-forming glioneuronal tumors arising from the spinal cord. A 44-year-old woman presented with a 15-year history of dissociated senso...

    journal_title:Human pathology

    pub_type: 杂志文章

    doi:10.1016/j.humpath.2008.11.010

    authors: Anan M,Inoue R,Ishii K,Abe T,Fujiki M,Kobayashi H,Goya T,Nakazato Y

    更新日期:2009-06-01 00:00:00

  • Silicone lymphadenopathy: a complication of silicone elastomer finger joint prostheses.

    abstract::Two cases of lymphadenopathy resulting from a lymph node foreign body reaction to silicone are presented. In each case lymph node biopsy was performed because of the clinical suspicion of neoplastic disease. Histologic study in both cases revealed no evidence of malignant disease, but rather demonstrated multinucleate...

    journal_title:Human pathology

    pub_type: 杂志文章

    doi:10.1016/s0046-8177(80)80005-0

    authors: Kircher T

    更新日期:1980-05-01 00:00:00

  • Ultrastructural features of cerebral amyloid angiopathy.

    abstract::Ultrastructural studies of the brain in three cases of cerebral amyloid angiopathy demonstrated that the vascular amyloid in this disease is composed of fibrils identical to those in systemic amyloid disease, mixed with a large amount of vesicular debris. Amyloid appears to be derived from circulating protein and is d...

    journal_title:Human pathology

    pub_type: 杂志文章

    doi:10.1016/s0046-8177(82)80251-7

    authors: Okoye MI,Watanabe I

    更新日期:1982-12-01 00:00:00

  • Identification of biomarkers to distinguish clear cell sarcoma from malignant melanoma.

    abstract::Clear cell sarcoma is a rare and malignant soft tissue tumor that shows phenotypic and immunohistochemical overlap with cutaneous malignant melanoma; identification of biomarkers that differentiate clear cell sarcoma from malignant melanoma is therefore needed. In this study, we performed mutation analysis of BRAF and...

    journal_title:Human pathology

    pub_type: 杂志文章

    doi:10.1016/j.humpath.2011.10.022

    authors: Yang L,Chen Y,Cui T,Knösel T,Zhang Q,Geier C,Katenkamp D,Petersen I

    更新日期:2012-09-01 00:00:00

  • Mucin antigen expression in gastric carcinomas of young and old adults.

    abstract::The striking differences in the histological features of gastric cancers in young and old adults have been thought to be related to differences in the biological behavior of these cancers. Recently, a new grading system (Goseki's classification) showed that the prognosis of the patient is particularly related to the m...

    journal_title:Human pathology

    pub_type: 杂志文章

    doi:10.1016/s0046-8177(97)90059-9

    authors: Sakamoto H,Yonezawa S,Utsunomiya T,Tanaka S,Kim YS,Sato E

    更新日期:1997-09-01 00:00:00

  • High proliferation rate and TNM stage but not histomorphological subtype are independent prognostic markers for overall survival in papillary renal cell carcinoma.

    abstract::Papillary renal cell carcinoma (PRCC) is currently divided in 2 subtypes. We reviewed a large cohort of PRCC and correlated subtype, morphological features and diagnostic marker expression with overall survival (OS) to uncover differences between the 2 subtypes. Three hundred seventy-six renal tumors initially diagnos...

    journal_title:Human pathology

    pub_type: 杂志文章

    doi:10.1016/j.humpath.2018.08.006

    authors: Polifka I,Agaimy A,Herrmann E,Spath V,Trojan L,Stöckle M,Becker F,Ströbel P,Wülfing C,Schrader AJ,Barth P,Staehler M,Stief C,Hohenfellner M,Macher-Göppinger S,Wullich B,Noldus J,Brenner W,Roos FC,Walter B,Otto W,

    更新日期:2019-01-01 00:00:00

  • Histological chorioamnionitis and neonatal leukemoid reaction in low-birth-weight infants.

    abstract::We investigated whether histological chorioamnionitis (HCA) is a risk factor predisposing to leukemoid reaction (LR) and whether LR is associated with the preterm parturition syndrome and the systemic fetal inflammation response syndrome. A prospective histological study on placentas was performed in preterm infants (...

    journal_title:Human pathology

    pub_type: 杂志文章

    doi:10.1016/j.humpath.2005.09.017

    authors: Zanardo V,Vedovato S,Trevisanuto DD,Suppiej A,Cosmi E,Fais GF,Chiarelli S

    更新日期:2006-01-01 00:00:00

  • Two case reports of childhood liver cell adenomas harboring beta-catenin abnormalities.

    abstract::The benign epithelial neoplasm liver cell adenoma is rare, especially in childhood. We report 2 such cases, 1 of which was associated with Prader-Willi syndrome. Differential diagnosis of the liver cell adenomas on the basis of histopathologic findings proved difficult and was based on the absence of cellular and nucl...

    journal_title:Human pathology

    pub_type: 杂志文章

    doi:10.1053/hupa.2002.125771

    authors: Takayasu H,Motoi T,Kanamori Y,Kitano Y,Nakanishi H,Tange T,Nakagawara A,Hashizume K

    更新日期:2002-08-01 00:00:00

  • Quantification of Leydig cells and stromal hyperplasia in the postmenopausal ovary of women with endometrial carcinoma.

    abstract::Endometrioid endometrial carcinomas (EECs) are correlated with high serum levels of androgens and estrogen. We hypothesized that Leydig cells and ovarian stromal hyperplasia contribute to postmenopausal ovarian androgen production and are observed more frequently in EEC patients. Ovaries of postmenopausal women with E...

    journal_title:Human pathology

    pub_type: 杂志文章

    doi:10.1016/j.humpath.2018.10.022

    authors: Reijnen C,Küsters-Vandevelde HVN,Abbink K,Zusterzeel PLM,van Herwaarden AE,van der Laak JAWM,Massuger LFAG,Snijders MPLM,Pijnenborg JMA,Bulten J

    更新日期:2019-03-01 00:00:00

  • Relation of neurological marker expression and EWS gene fusion types in MIC2/CD99-positive tumors of the Ewing family.

    abstract::The Ewing family of tumors (EFT) is characterized by high MIC2/CD99 expression and specific EWS/ETS gene rearrangements, resulting in different chimeric transcripts. Further division into peripheral primitive neuroectodermal tumors and Ewing's sarcoma is still debated and, in the absence of distinct morphological para...

    journal_title:Human pathology

    pub_type: 杂志文章

    doi:10.1016/s0046-8177(99)90223-x

    authors: Amann G,Zoubek A,Salzer-Kuntschik M,Windhager R,Kovar H

    更新日期:1999-09-01 00:00:00

  • Primary multipotential malignant neoplasm of bone: chondrosarcoma associated with squamous cell carcinoma.

    abstract::A primary neoplasm of the proximal humerus in a 68-year-old woman was unique histologically in that it contained both malignant cartilaginous and squamous cell components. The epithelial differentiation was confirmed by the demonstration of keratin by immunohistochemical techniques and of basement membrane, tonofilame...

    journal_title:Human pathology

    pub_type: 杂志文章

    doi:10.1016/s0046-8177(83)80227-5

    authors: Ling LL,Steiner GC

    更新日期:1986-03-01 00:00:00

  • Spindle cell squamous carcinoma of the esophagus: analysis of ploidy and tumor proliferative activity in a series of 13 cases.

    abstract::Spindle cell squamous esophageal carcinomas are distinctive polypoid "biphasic" tumors in which the sarcoma-like phenotype usually predominates over the epithelial component. To biologically assess both phenotypes, we compared the tumoral proliferative activity and DNA ploidy between the two histological components of...

    journal_title:Human pathology

    pub_type: 杂志文章

    doi:10.1016/s0046-8177(98)90458-0

    authors: Lauwers GY,Grant LD,Scott GV,Carr NJ,Sobin LH

    更新日期:1998-08-01 00:00:00

  • Mammary and vaginal myofibroblastomas are genetically related lesions: fluorescence in situ hybridization analysis shows deletion of 13q14 region.

    abstract::Partial monosomy 13q, a chromosomal alteration originally reported in spindle cell lipoma, has also been documented in a few cases of mammary myofibroblastoma. Subsequently, a monoallelic loss of RB1 and FOXO1, located on 13q14, was identified in some cases of cellular angiofibroma, a benign stromal tumor of the lower...

    journal_title:Human pathology

    pub_type: 杂志文章,多中心研究

    doi:10.1016/j.humpath.2012.01.015

    authors: Magro G,Righi A,Casorzo L,Antonietta T,Salvatorelli L,Kacerovská D,Kazakov D,Michal M

    更新日期:2012-11-01 00:00:00

  • Specimen collection and transport.

    abstract::The approach to the collection and transport of meaningful and accurate microbiologic specimens depends upon the nature of the suspected infectious entity and upon the location of the pathogenic process, as well as the possession of appropriate instrumentation and containers. New information and newly developed collec...

    journal_title:Human pathology

    pub_type: 杂志文章,评审

    doi:10.1016/s0046-8177(76)80040-8

    authors: Matsen JM,Ederer GM

    更新日期:1976-05-01 00:00:00

  • The ultrastructure of small-cell osteosarcoma, with a review of the light microscopy and differential diagnosis.

    abstract::Small-cell osteosarcoma is a rare form of osteosarcoma and often poses difficulty in differential diagnosis when tissue samples do not include any diagnostic osteoid. By light microscopy, it may be difficult to distinguish small-cell osteosarcoma from other small-cell neoplasms, especially Ewing's sarcoma and mesenchy...

    journal_title:Human pathology

    pub_type: 杂志文章

    doi:10.1016/0046-8177(91)90161-h

    authors: Dickersin GR,Rosenberg AE

    更新日期:1991-03-01 00:00:00

  • Pseudogynecomastia due to neurofibromatosis--a light microscopic and ultrastructural study.

    abstract::A six year old boy with bilateral breast enlargement was found to have a normal endocrine status. Resected tissue revealed the features of pseudogynecomastia due to a proliferation of fibrous tissue traversed by neuroid structures. Multinucleated giant cells were present within the fibrous tissue. Ultrastructural stud...

    journal_title:Human pathology

    pub_type: 杂志文章

    doi:10.1016/s0046-8177(81)80180-3

    authors: Lipper S,Willson CF,Copeland KC

    更新日期:1981-08-01 00:00:00

  • Prominent intrasinusoidal infiltration of the bone marrow by mantle cell lymphoma.

    abstract::Intrasinusoidal infiltration of bone marrow (BM) may accompany several malignant lymphoproliferative disorders. In small B-cell lymphomas, this pattern is considered specific for splenic marginal zone lymphoma (SMZL) when exclusive or prominent, although it may occur in other subtypes of non-Hodgkin's lymphomas (NHLs)...

    journal_title:Human pathology

    pub_type: 杂志文章

    doi:10.1016/s0046-8177(03)00286-7

    authors: Schenka AA,Gascoyne RD,Duchayne E,Delsol G,Brousset P

    更新日期:2003-08-01 00:00:00