当前位置： SCI文献检索 > DIABETOLOGIA期刊下所有文献 > Influence of protamine on adhesion, chemotaxis and proliferation of human vascular smooth muscle cells.

Influence of protamine on adhesion, chemotaxis and proliferation of human vascular smooth muscle cells.

Abstract：

:It has been shown that, in streptozotocin diabetic rats, protamine-retarded insulin administered in vivo stimulates intimal hyperplasia in balloon-injured carotid artery. The aim of this study was to evaluate the influence of protamine on cultured human vascular smooth muscle cells (h VSMC), by observing its effects on adhesion, chemotaxis and proliferation. hVSMC were isolated during abdominal surgery, cultured and utilized at passages 6-10. We observed that protamine stimulates: 1) cell adhesion in the concentration range 0.04-20 micrograms/ml (analysis of variance, ANOVA, p < 0.0001); 2) cell chemotaxis in the absence of fetal calf serum (FCS) in the concentration range 1-200 micrograms/ml (ANOVA, p < 0.0001) and in the presence of 1% FCS in the concentration range 5-200 micrograms/ml (ANOVA, p < 0.0001), further enhancing the chemotaxis induced by 10% FCS in the concentration range 20-200 micrograms/ml (ANOVA, p < 0.0001); 3) cell proliferation and 3H-thymidine incorporation from 1 to 5 micrograms/ml (ANOVA, p < 0.0001); 4) cell c-fos oncoprotein nuclear expression. We also observed that protamine effects on chemotaxis, proliferation and c-fos expression are inhibited by heparin that human insulin stimulates cell proliferation and 3H-thymidine incorporation (ANOVA, p < 0.0001) at concentrations equal to or greater than 480 pmol/l and that these effects of insulin persist in the presence of protamine. In conclusion, protamine influences hVSMC behaviour by interfering with biological functions involved in atherogenesis. The concentrations used in this short-term in vitro study were higher than those probably occurring in vivo in patients chronically treated by protamine-retarded insulin preparations: further studies, therefore, are needed to evaluate the safety of protamine as a retardant of insulin action in vivo.

journal_name

Diabetologia

journal_title

Diabetologia

authors

Cavalot F,Russo I,Mattiello L,Anfossi G,Massucco P,Mularoni E,Hahn AW,Trovati M

doi

10.1007/s001250050644

subject

Has Abstract

pub_date

1997-01-01 00:00:00

pages

67-75

issue

1

eissn

0012-186X

issn

1432-0428

journal_volume

40

pub_type

杂志文章

相关文献

DIABETOLOGIA文献大全
  • IRS1 degradation and increased serine phosphorylation cannot predict the degree of metabolic insulin resistance induced by oxidative stress.

    abstract：AIM/HYPOTHESIS:Oxidative stress was shown to selectively induce impaired metabolic response to insulin, raising the possible involvement of alterations in Insulin-Receptor-Substrate (IRS) proteins. This study was conducted to assess whether oxidative stress induced IRS protein degradation and enhanced serine phosphoryl...

    journal_title：Diabetologia

    pub_type： 杂志文章

    doi：10.1007/s00125-003-1097-5

    authors： Potashnik R,Bloch-Damti A,Bashan N,Rudich A

    更新日期：2003-05-01 00:00:00

  • SDF-1-CXCR4 differentially regulates autoimmune diabetogenic T cell adhesion through ROBO1-SLIT2 interactions in mice.

    abstract：AIMS/HYPOTHESIS:We had previously reported that stromal cell-derived factor 1 (SDF-1) mediates chemorepulsion of diabetogenic T cell adhesion to islet microvascular endothelium through unknown mechanisms in NOD mice. Here we report that SDF-1-mediated chemorepulsion occurs through slit homologue (SLIT)2-roundabout, axo...

    journal_title：Diabetologia

    pub_type： 杂志文章

    doi：10.1007/s00125-013-2978-x

    authors： Glawe JD,Mijalis EM,Davis WC,Barlow SC,Gungor N,McVie R,Kevil CG

    更新日期：2013-10-01 00:00:00

  • High heritability and genetic correlation of intravenous glucose- and tolbutamide-induced insulin secretion among non-diabetic family members of type 2 diabetic patients.

    abstract：AIMS/HYPOTHESIS:The aim of this study was to estimate the heritability of quantitative measures of glucose regulation obtained from a tolbutamide-modified frequently sampled IVGTT (t-FSIGT) and to correlate the heritability of the glucose-stimulated beta cell response to the tolbutamide-induced beta cell response. In a...

    journal_title：Diabetologia

    pub_type： 杂志文章

    doi：10.1007/s00125-014-3207-y

    authors： Gjesing AP,Hornbak M,Allin KH,Ekstrøm CT,Urhammer SA,Eiberg H,Pedersen O,Hansen T

    更新日期：2014-06-01 00:00:00

  • Higher circulating omentin is associated with increased risk of primary cardiovascular events in individuals with diabetes.

    abstract：AIMS/HYPOTHESIS:Higher concentrations of the adipokine omentin are associated with lower levels of cardiometabolic risk factors in experimental and cross-sectional studies, but with higher risk of type 2 diabetes and cardiovascular diseases in population-based cohort studies. However, it is unknown whether high omentin...

    journal_title：Diabetologia

    pub_type： 杂志文章

    doi：10.1007/s00125-019-05017-2

    authors： Niersmann C,Carstensen-Kirberg M,Maalmi H,Holleczek B,Roden M,Brenner H,Herder C,Schöttker B

    更新日期：2020-02-01 00:00:00

  • Hepatic glucose and lipid metabolism.

    abstract：:The liver has a central role in the regulation of systemic glucose and lipid fluxes during feeding and fasting and also relies on these substrates for its own energy needs. These parallel requirements are met by coordinated control of carbohydrate and lipid fluxes into and out of the Krebs cycle, which is highly tuned...

    journal_title：Diabetologia

    pub_type： 杂志文章,评审

    doi：10.1007/s00125-016-3940-5

    authors： Jones JG

    更新日期：2016-06-01 00:00:00

  • A systems view of type 2 diabetes-associated metabolic perturbations in saliva, blood and urine at different timescales of glycaemic control.

    abstract：AIMS/HYPOTHESIS:Metabolomics has opened new avenues for studying metabolic alterations in type 2 diabetes. While many urine and blood metabolites have been associated individually with diabetes, a complete systems view analysis of metabolic dysregulations across multiple biofluids and over varying timescales of glycaem...

    journal_title：Diabetologia

    pub_type： 杂志文章

    doi：10.1007/s00125-015-3636-2

    authors： Yousri NA,Mook-Kanamori DO,Selim MM,Takiddin AH,Al-Homsi H,Al-Mahmoud KA,Karoly ED,Krumsiek J,Do KT,Neumaier U,Mook-Kanamori MJ,Rowe J,Chidiac OM,McKeon C,Al Muftah WA,Kader SA,Kastenmüller G,Suhre K

    更新日期：2015-08-01 00:00:00

  • Collagen I induction by high glucose levels is mediated by epidermal growth factor receptor and phosphoinositide 3-kinase/Akt signalling in mesangial cells.

    abstract：AIMS/HYPOTHESIS:Glomerular matrix accumulation is a hallmark of diabetic nephropathy. Recent data have linked the serine/threonine kinase protein kinase B (Akt) to matrix modulation. Here, we studied its role in high glucose-induced collagen elaboration by mesangial cells. METHODS:Primary rat mesangial cells were trea...

    journal_title：Diabetologia

    pub_type： 杂志文章

    doi：10.1007/s00125-007-0721-1

    authors： Wu D,Peng F,Zhang B,Ingram AJ,Gao B,Krepinsky JC

    更新日期：2007-09-01 00:00:00

  • FGF21 improves glucose homeostasis in an obese diabetes-prone mouse model independent of body fat changes.

    abstract：AIMS/HYPOTHESIS:Fibroblast growth factor 21 (FGF21) is considered to be a promising therapeutic candidate for the treatment of type 2 diabetes. However, as FGF21 levels are elevated in obese and diabetic conditions we aimed to test if exogenous FGF21 is sufficient to prevent diabetes and beta cell loss in New Zealand o...

    journal_title：Diabetologia

    pub_type： 杂志文章

    doi：10.1007/s00125-017-4389-x

    authors： Laeger T,Baumeier C,Wilhelmi I,Würfel J,Kamitz A,Schürmann A

    更新日期：2017-11-01 00:00:00

  • Glycogen synthase kinase 3 inhibition improves insulin-stimulated glucose metabolism but not hypertension in high-fat-fed C57BL/6J mice.

    abstract：AIMS/HYPOTHESIS:In the current study, the effect of a highly specific peptide inhibitor of glycogen synthase kinase 3 (GSK3) (L803-mts) on glucose metabolism and BP was examined in a high-fat (HF) fed mouse model of diabetes. METHODS:C57/BL6J mice were placed on an HF diet for 3 months and treated with L803-mts for 20...

    journal_title：Diabetologia

    pub_type： 杂志文章

    doi：10.1007/s00125-006-0552-5

    authors： Rao R,Hao CM,Redha R,Wasserman DH,McGuinness OP,Breyer MD

    更新日期：2007-02-01 00:00:00

  • Correction to: Chronomedicine and type 2 diabetes: shining some light on melatonin.

    abstract：:The authors have been made aware that the following sentence is incorrect: 'Like IIK7, both ramelteon and tasimelteon have a greater affinity for the MT2 receptor [162].' ...

    journal_title：Diabetologia

    pub_type： 杂志文章,已发布勘误

    doi：10.1007/s00125-018-4577-3

    authors： Forrestel AC,Miedlich SU,Yurcheshen M,Wittlin SD,Sellix MT

    更新日期：2018-05-01 00:00:00

  • Low serum 25-hydroxyvitamin D level predicts progression to type 2 diabetes in individuals with prediabetes but not with normal glucose tolerance.

    abstract：AIMS/HYPOTHESIS:Vitamin D deficiency may increase the risk of type 2 diabetes. We therefore investigated whether serum concentrations of 25-hydroxyvitamin D [25(OH)D] would predict the development of prediabetes (impaired fasting glucose, impaired glucose tolerance or the two combined) and type 2 diabetes, either on th...

    journal_title：Diabetologia

    pub_type： 杂志文章

    doi：10.1007/s00125-012-2529-x

    authors： Deleskog A,Hilding A,Brismar K,Hamsten A,Efendic S,Östenson CG

    更新日期：2012-06-01 00:00:00

  • Altered pattern of insulin receptor isotypes in skeletal muscle membranes of type 2 (non-insulin-dependent) diabetic subjects.

    abstract：:The human insulin receptor exists in two isoforms (HIR-A alpha-subunit 719 amino acids and HIR-B alpha-subunit 731 amino acids) which are generated by alternative splicing of a small exon and display distinct patterns of tissue-specific expression. Using the polymerase chain reaction we have recently shown that skelet...

    journal_title：Diabetologia

    pub_type： 杂志文章

    doi：10.1007/BF00404072

    authors： Kellerer M,Sesti G,Seffer E,Obermaier-Kusser B,Pongratz DE,Mosthaf L,Häring HU

    更新日期：1993-07-01 00:00:00

  • Implantation of standardized beta-cell grafts in a liver segment of IDDM patients: graft and recipients characteristics in two cases of insulin-independence under maintenance immunosuppression for prior kidney graft.

    abstract：:Islet allografts in insulin-dependent diabetic (IDDM) patients exhibit variable survival lengths and low rates of insulin-independence despite treatment with anti-T-cell antibodies and maintenance immunosuppression. Use of poorly characterized freshly isolated preparations makes it difficult to determine whether failu...

    journal_title：Diabetologia

    pub_type： 临床试验,杂志文章,多中心研究

    doi：10.1007/s001250050929

    authors： Keymeulen B,Ling Z,Gorus FK,Delvaux G,Bouwens L,Grupping A,Hendrieckx C,Pipeleers-Marichal M,Van Schravendijk C,Salmela K,Pipeleers DG

    更新日期：1998-04-01 00:00:00

  • Reversal of type 2 diabetes: normalisation of beta cell function in association with decreased pancreas and liver triacylglycerol.

    abstract：AIMS/HYPOTHESIS:Type 2 diabetes is regarded as inevitably progressive, with irreversible beta cell failure. The hypothesis was tested that both beta cell failure and insulin resistance can be reversed by dietary restriction of energy intake. METHODS:Eleven people with type 2 diabetes (49.5 ± 2.5 years, BMI 33.6 ± 1.2 ...

    journal_title：Diabetologia

    pub_type： 临床试验,杂志文章

    doi：10.1007/s00125-011-2204-7

    authors： Lim EL,Hollingsworth KG,Aribisala BS,Chen MJ,Mathers JC,Taylor R

    更新日期：2011-10-01 00:00:00

  • A developmental nephron deficit in rats is associated with increased susceptibility to a secondary renal injury due to advanced glycation end-products.

    abstract：AIMS/HYPOTHESIS:The aim of this study was to investigate the effects of a secondary renal insult, due to chronic infusion of AGEs on renal function, and on early pathological markers in rats with a developmental nephron deficit. METHODS:Female Wistar-Kyoto rats were fed a low-protein diet (LPD; 8.7% casein) or a norma...

    journal_title：Diabetologia

    pub_type： 杂志文章

    doi：10.1007/s00125-006-0175-x

    authors： Zimanyi MA,Denton KM,Forbes JM,Thallas-Bonke V,Thomas MC,Poon F,Black MJ

    更新日期：2006-04-01 00:00:00

  • Prevalence of diabetes mellitus in north-western Tanzania.

    abstract：:The prevalence of diabetes mellitus in 3,145 Tanzanian Africans living in three different areas of the country was studied. Fasting capillary blood glucose concentrations were measured by Dextrometer and if the levels were greater than or equal to 5.5 mmol/l an oral glucose tolerance test was performed. When using the...

    journal_title：Diabetologia

    pub_type： 杂志文章

    doi：10.1007/BF00266032

    authors： Ahrén B,Corrigan CB

    更新日期：1984-05-01 00:00:00

  • Bivariate genome-wide scan for metabolic phenotypes in non-diabetic Chinese individuals from the Stanford, Asia and Pacific Program of Hypertension and Insulin Resistance Family Study.

    abstract：AIMS/HYPOTHESIS:Hypertension, obesity, impaired glucose tolerance and dyslipidaemia are metabolic abnormalities that often cluster together more often than expected by chance alone. Since these metabolic variables are highly heritable and are at least partially genetically determined, the clustering of defects in these...

    journal_title：Diabetologia

    pub_type： 杂志文章

    doi：10.1007/s00125-007-0720-2

    authors： Chiu YF,Chuang LM,Kao HY,Ho LT,Ting CT,Hung YJ,Chen YD,Donlon T,Curb JD,Quertermous T,Hsiung CA

    更新日期：2007-08-01 00:00:00

  • Influence of a transplantable insulinoma on the pancreatic status of insulin and pancreatic polypeptide in the rat.

    abstract：:The effect of endogenous hyperinsulinaemia, produced by syngeneic transplantation of rat insulinoma at an extrapancreatic site, on pancreatic insulin and pancreatic polypeptide has been examined by radioimmunoassay and immunohistochemistry. Twenty days after subcutaneous transplantation, tumour-bearing rats exhibited ...

    journal_title：Diabetologia

    pub_type： 杂志文章

    doi：10.1007/BF00273864

    authors： O'Hare MM,Shaw C,Swanston-Flatt SK,Marcelli M,Buchanan KD,Flatt PR

    更新日期：1985-03-01 00:00:00

  • The transcription factor STAT6 plays a critical role in promoting beta cell viability and is depleted in islets of individuals with type 1 diabetes.

    abstract：AIMS/HYPOTHESIS:In type 1 diabetes, selective beta cell loss occurs within the inflamed milieu of insulitic islets. This milieu is generated via the enhanced secretion of proinflammatory cytokines and by the loss of anti-inflammatory molecules such as IL-4 and IL-13. While the actions of proinflammatory cytokines have ...

    journal_title：Diabetologia

    pub_type： 杂志文章

    doi：10.1007/s00125-018-4750-8

    authors： Leslie KA,Russell MA,Taniguchi K,Richardson SJ,Morgan NG

    更新日期：2019-01-01 00:00:00

  • Effect of advanced glycation end products on oxidative stress in endothelial cells in culture: a warning on the use of cells studied in serum-free media.

    abstract：AIMS/HYPOTHESIS:Alterations in vascular permeability and oxidative stress are characteristics of endothelial dysfunction in diabetic vascular disease. Since AGE-proteins have been hypothesized to mediate these effects, we studied the effects of AGE-bovine serum albumin on endothelial monolayer permeability and intracel...

    journal_title：Diabetologia

    pub_type： 杂志文章

    doi：10.1007/s001250100646

    authors： Hui YY,McAmis WC,Baynes JW,Schaeffer RC Jr,Wolf MB

    更新日期：2001-10-01 00:00:00

  • A high concentration of prorenin in early pregnancy is associated with development of pre-eclampsia in women with type 1 diabetes.

    abstract：AIMS/HYPOTHESIS:The aim of this study was to investigate whether components of the renin-angiotensin system and semicarbazide-sensitive amine oxidase (SSAO) are associated with the development of pre-eclampsia in women with type 1 diabetes. METHODS:This was an observational study of 107 consecutive pregnant women with...

    journal_title：Diabetologia

    pub_type： 杂志文章

    doi：10.1007/s00125-011-2087-7

    authors： Ringholm L,Pedersen-Bjergaard U,Thorsteinsson B,Boomsma F,Damm P,Mathiesen ER

    更新日期：2011-07-01 00:00:00

  • Regulation by cytokines of the inducible nitric oxide synthase promoter in insulin-producing cells.

    abstract：:Cytokines could contribute to beta-cell damage in Type I diabetes mellitus. The radical nitric oxide, generated by the inducible form of nitric oxide synthase (iNOS), is a potential mediator of cytokine-induced beta-cell dysfunction. In rat pancreatic islets and insulin-producing cell lines, interleukin-1beta (IL-1bet...

    journal_title：Diabetologia

    pub_type： 杂志文章

    doi：10.1007/s001250051036

    authors： Darville MI,Eizirik DL

    更新日期：1998-09-01 00:00:00

  • Raised serum apolipoprotein (a) in active diabetic retinopathy.

    abstract：:Progressive capillary occlusion often leads to severe retinopathy within 15-20 years of the onset of Type 1 (insulin-dependent) diabetes mellitus. Lipoprotein(a), a complex formed by apolipoprotein(a), apo B-100 and lipids, is considered an independent, genetically determined, predictor of cardiovascular disease. It m...

    journal_title：Diabetologia

    pub_type： 杂志文章

    doi：10.1007/BF00399100

    authors： Maioli M,Tonolo G,Pacifico A,Ciccarese M,Brizzi P,Kohner EM,Porta M

    更新日期：1993-01-01 00:00:00

  • The insulin dilemma in resource-limited countries. A way forward?

    abstract：:The International Insulin Foundation (IIF) has developed and validated a needs-assessment instrument called the Rapid Assessment Protocol for Insulin Access (RAPIA) which has been used in seven countries in four continents to analyse the constraints to delivering effective continuing care for people with diabetes. One...

    journal_title：Diabetologia

    pub_type： 杂志文章

    doi：10.1007/s00125-010-1897-3

    authors： Gill GV,Yudkin JS,Keen H,Beran D

    更新日期：2011-01-01 00:00:00

  • Treatment of diabetes mellitus.

    abstract：:New treatment strategies for subjects with Type 1 (insulin-dependent) and Type 2 (non-insulin-dependent) diabetes mellitus are being developed. Pilot studies utilising insulin itself have been reported to prevent Type 1 diabetes in subjects likely, by immunogenetic and physiologic criteria, to develop clinically overt...

    journal_title：Diabetologia

    pub_type： 杂志文章,评审

    doi：10.1007/BF00586274

    authors： Robertson RP,Klein DJ

    更新日期：1992-12-01 00:00:00

  • Fatty liver and obesity: phenotypically correlated but genetically distinct traits in a mouse model of type 2 diabetes.

    abstract：AIMS/HYPOTHESIS:Obesity and fatty liver are commonly associated with type 2 diabetes, but the genetic and functional bases linking fatty liver with obesity and diabetes are largely unknown. Our aim was to investigate the association of fatty liver with obesity and other diabetes-related phenotypes and to define the gen...

    journal_title：Diabetologia

    pub_type： 杂志文章

    doi：10.1007/s00125-007-0700-6

    authors： Itoi-Babaya M,Ikegami H,Fujisawa T,Ueda H,Nojima K,Babaya N,Kobayashi M,Noso S,Kawaguchi Y,Yamaji K,Shibata M,Ogihara T

    更新日期：2007-08-01 00:00:00

  • A population-based study of the association between areca nut chewing and type 2 diabetes mellitus in men (Keelung Community-based Integrated Screening programme No. 2).

    abstract：AIMS/HYPOTHESIS:The aim of this study was to assess whether the diabetogenicity of areca nut (Areca catechu or 'betel-nut'), which has previously been demonstrated experimentally in mice, independently contributes to the risk of hyperglycaemia or type 2 diabetes in men in Taiwan, where the habit has become established ...

    journal_title：Diabetologia

    pub_type： 杂志文章

    doi：10.1007/s00125-004-1532-2

    authors： Tung TH,Chiu YH,Chen LS,Wu HM,Boucher BJ,Chen TH,Keelung Community-based Integrated Screening programme No. 2.

    更新日期：2004-10-01 00:00:00

  • Short-term moderate sodium restriction induces relative hyperfiltration in normotensive normoalbuminuric Type I diabetes mellitus.

    abstract：AIMS/HYPOTHESIS:Type I (insulin-dependent) diabetes mellitus is associated with an increased extracellular volume. Sodium restriction might seem a logical form of treatment but data on its renal effects is conflicting. We therefore studied the effects of sodium restriction on renal haemodynamics in uncomplicated Type I...

    journal_title：Diabetologia

    pub_type： 临床试验,杂志文章,随机对照试验

    doi：10.1007/s00125-001-0763-8

    authors： Luik PT,Hoogenberg K,Van Der Kleij FG,Beusekamp BJ,Kerstens MN,De Jong PE,Dullaart RP,Navis GJ

    更新日期：2002-04-01 00:00:00

  • Islet amyloid polypeptide (amylin) and insulin are differentially expressed in chronic diabetes induced by streptozotocin in rats.

    abstract：:Islet amyloid polypeptide (IAPP) is overexpressed relative to insulin under several experimental conditions relevant to diabetes mellitus, including the immediate phase (7 days) following induction of streptozotocin diabetes. In the present study, IAPP and insulin gene expression were examined in chronic streptozotoci...

    journal_title：Diabetologia

    pub_type： 杂志文章

    doi：10.1007/BF00418536

    authors： Mulder H,Ahrén B,Sundler F

    更新日期：1996-06-01 00:00:00

  • The role of the p75 neurotrophin receptor in the morphology of dorsal root ganglion cells in streptozotocin diabetic mice: effects of sciatic nerve crush.

    abstract：AIMS/HYPOTHESIS:Neuronal dorsal root ganglion (DRG) cells seem to be vulnerable in diabetes. The aim of this study was to determine whether the p75 neurotrophin receptor stimulates perikaryal shrinkage and neuronal death, and further accelerates neuronal DRG cell loss after axotomy in a mouse model of diabetes. METHOD...

    journal_title：Diabetologia

    pub_type： 杂志文章

    doi：10.1007/s00125-004-1504-6

    authors： Jiang Y,Jakobsen J

    更新日期：2004-09-01 00:00:00