Abstract:
:Serial virus specimens rescued from rabbits, experimentally infected with bovine immunodeficiency (BIV) strain R29, were monitored for changes in quasispecies population, using the single-strand conformation polymorphism (SSCP) analysis. The generation of characteristic SSCP patterns enables the rapid differentiation of BIV variants derived from the conserved part on the env region of the BIV genome, reducing the need for expensive and time-consuming direct sequencing analyses. Our results showed genetic polymorphism among a number of sampled BIV population in experimentally infected rabbits. At least three SSCP patterns (BIV quasispecies) were detected. The SSCP analysis allows for an easy, sensitive, and rapid screening of genetic variants of the virus and the assessment of variation at a number of tissue target sites. These variations may relate to cell-type targets and/or disease progression, and could be significant to our understanding of lentiviral pathogenesis.
journal_name
Viral Immunoljournal_title
Viral immunologyauthors
Kalvatchev Z,Walder R,Garzaro D,Barrios Mdoi
10.1089/08828240050144680subject
Has Abstractpub_date
2000-01-01 00:00:00pages
373-81issue
3eissn
0882-8245issn
1557-8976journal_volume
13pub_type
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