Diagnosis of peripheral T-cell lymphoma by fine-needle aspiration biopsy: a cytomorphologic and immunophenotypic approach.

Abstract:

:The diagnosis of T-cell lymphomas by fine-needle aspiration biopsies (FNAB) is extremely difficult. This is mainly due to the rarity of the disease, the morphologic similarity to reactive lymphadenopathy, and the difficulty in identifying abnormal T-cell antigen expression. We studied FNAB of histologically proven T-cell lymphomas in an attempt to identify the salient cytomorphologic features as well as the surface marker attributes of the disease. Twenty cases were reviewed. The smears were evaluated for overall cytologic pattern and percentage of abnormal cells. A critical review of flow cytometric (FCM) antigen expression of the lymphomas was also performed. There were 6 female and 14 male patients, with an age range of 9-84 yr (median, 36 yr). Fourteen cases (70%) showed polymorphous smears, and 6 cases (30%) showed monomorphous smears. Abnormal cells ranged from 10-100% (median, 60%). Abnormal T-cell antigen expression by FCM analysis was seen in 17 cases (85%). The most common aberrant T-cell antigen pattern was loss of 3 or more pan-T-cell antigens (n = 10). The most common individual T-cell antigen loss was that of CD7 (n = 10), followed by loss of CD5 (n = 5). There was also loss of CD4 and CD8 (n = 5), loss of CD5 and CD7 (n = 5), complete loss of CD3 (n = 4), coexpression of CD4 and CD8 (n = 1), and partial loss of CD3 (n = 1). CD56 was expressed in 2 cases. CD1a was tested in one case and was positive. CD4/CD8 ratio was elevated (>2.5) in 9 cases (53%), with a range of 3/1-57/1 (median, 12/1). TCR gene rearrangement using PCR was positive in 7 of 9 tested cases. Our findings suggest that the diagnosis of peripheral T-cell lymphomas can be achieved by FNAB in the majority of cases through close analysis of the morphology. This can be supported by a critical analysis of the phenotype using two or three-color flow cytometry with an attempt at identification of one or more abnormal T-cell antigen expression and/or loss. This can be supplemented by CD4/CD8 ratios and T-cell receptor gene rearrangement analysis.

journal_name

Diagn Cytopathol

journal_title

Diagnostic cytopathology

authors

Al Shanqeety O,Mourad WA

doi

10.1002/1097-0339(200012)23:6<375::aid-dc2>3.0.co;

subject

Has Abstract

pub_date

2000-12-01 00:00:00

pages

375-9

issue

6

eissn

8755-1039

issn

1097-0339

pii

10.1002/1097-0339(200012)23:6<375::AID-DC2>3.0.CO;

journal_volume

23

pub_type

杂志文章
  • Intranuclear peudoinclusions: morphology, pathogenesis, and significance.

    abstract::Intranuclear pseudoinclusions represent invaginations of the cytoplasm into the nucleus. The nuclear pseudoinclusions are generally easier to appreciate in cytological preparations than frozen sections or histopathology thus forming an important cytological feature. In the present article we have reviewed the morpholo...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章,评审

    doi:10.1002/dc.21714

    authors: Arora SK,Dey P

    更新日期:2012-08-01 00:00:00

  • Cardiac angiosarcoma: report of a case diagnosed by echocardiographic-guided fine-needle aspiration.

    abstract::The authors present a case of cardiac angiosarcoma diagnosed by fine-needle aspiration (FNA) in a 33-year-old male. A percutaneous right atrial mass biopsy was performed via a right internal jugular venous approach and under echocardiographic guidance. FNA showed pleomorphic spindle-shaped cells present singly and in ...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.20532

    authors: Bhalla R,Nassar A

    更新日期:2007-03-01 00:00:00

  • Pancreatic adenocarcinoma: regression analysis to identify improved cytologic criteria.

    abstract::The incidence of pancreatic adenocarcinoma is increasing and it is usually unresectable at the time of diagnosis. Consequently, fine-needle aspiration biopsy (FNAB) is being used more frequently for diagnosis. The reported sensitivity of diagnosing pancreatic adenocarcinoma by FNAB has varied between 50% and 100%. In ...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.2840070404

    authors: Cohen MB,Egerter DP,Holly EA,Ahn DK,Miller TR

    更新日期:1991-01-01 00:00:00

  • Cytodiagnosis of nipple discharge: a study of 602 samples from 484 cases.

    abstract::To find out the gross and microscopic differentiating features between nipple discharges (ND) due to various breast lesions, smears of 602 ND samples from 484 cases were reviewed by one of the investigators (D.K.D.). The reviewed cytodiagnoses were as follows: benign nipple discharge (59.1%), inflammatory ND (6.5%), ?...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.1098

    authors: Das DK,Al-Ayadhy B,Ajrawi MT,Shaheen AA,Sheikh ZA,Malik M,Pathan SK,Ebrahim B,Francis IM,Satar SA,Abdulla MA,Luthra UK,Junaid TA

    更新日期:2001-07-01 00:00:00

  • Aspiration cytology of clear-cell lesions of the parotid gland: morphologic features and differential diagnosis.

    abstract::Clear-cell lesions of the parotid gland are uncommon but when studied by fine-needle aspiration may result in a clinically important but cytologically difficult differential diagnosis. Clear-cell lesions involving the parotid include acinic cell neoplasm, clear cell oncocytoma, mucoepidermoid carcinoma, primary clear-...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.2840090621

    authors: Layfield LJ,Glasgow BJ

    更新日期:1993-12-01 00:00:00

  • Fine-needle aspiration biopsy of a case of breast carcinoma with choriocarcinomatous features.

    abstract::Breast disease is of great concern to patients and health care providers alike. Malignancies of the breast and their various presentations can pose tremendous challenges with respect to early diagnosis and effective treatment. Carcinoma of the breast may appear in several forms and produce one or more ectopic substanc...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章,评审

    doi:10.1002/dc.20485

    authors: Siddiqui NH,Cabay RJ,Salem F

    更新日期:2006-10-01 00:00:00

  • Cytodiagnostic patterns of metaplastic breast carcinoma in aspiration samples: a study of 14 cases.

    abstract::Metaplastic breast carcinomas (MBCs) are not only uncommon neoplasms but also show a myriad of cytomorphologic patterns in aspiration samples. These tumors comprise less than 5% of breast malignancies and have an uncertain prognosis. In this study, the fine-needle aspiration cytologic features of 14 MBCs with the iden...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/(sici)1097-0339(199901)20:1<10::aid-dc3>3.

    authors: Gupta RK

    更新日期:1999-01-01 00:00:00

  • Flow cytometric analysis of fine needle aspirates is affected by tumor subtype, but not by anatomic location nor technique.

    abstract:BACKGROUND:Current clinical practices are shifting towards utilizing less invasive biopsy techniques, including fine needle aspiration (FNA) and needle core biopsies. If a patient has a suspected hematologic malignancy, a portion of the FNA sample is typically submitted for flow cytometry (FC) analysis, providing valua...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.24417

    authors: Wake LM,VandenBussche CJ,Ali SZ,Wagner-Johnston ND,Burns KH,Gocke CD,Vuica-Ross M,Borowitz MJ,Duffield AS

    更新日期:2020-06-01 00:00:00

  • Fine-needle aspiration cytology of malignant nerve sheath tumors.

    abstract::Precise preoperative diagnosis of three malignant nerve sheath tumors (MNST) was based on their remarkably uniform and highly characteristic cytologic appearance. The differential diagnosis with benign nerve sheath tumors and other spindle cell sarcomas is addressed, and the possibility of achieving confident diagnosi...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.2840080603

    authors: Vendraminelli R,Cavazzana AO,Poletti A,Galligioni A,Pennelli N

    更新日期:1992-01-01 00:00:00

  • Mesothelioma: you do not have to work for it.

    abstract::Asbestos pollution is a global problem. Asbestos exposure induced mesothelioma does not require an 'occupational' type of exposure. Bystander exposures may result in earlier age of disease onset and more aggressive disease progression as described in the following 3 case reports. ...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.20766

    authors: Ampleford EJ,Ohar J

    更新日期:2007-12-01 00:00:00

  • Giant adrenal cortical carcinoma, clinically "nonfunctional": report of a case containing cytoplasmic hyaline globules of vimentin.

    abstract::Adrenal cortical carcinoma (ACC) is a rare neoplasm that affects all age groups, with a bimodal peak of incidence, in young individuals in the first decade or two of life and in older subjects in the fifth to seventh decades. It may be clinically "functional" with Cushing's syndrome, virilization, or feminization, or ...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/(sici)1097-0339(199912)21:6<394::aid-dc5>3

    authors: Laforga JB,Aranda FI

    更新日期:1999-12-01 00:00:00

  • Aspiration biopsy of nodular pseudoangiomatous stromal hyperplasia of the breast: clinicopathologic correlates in 10 cases.

    abstract::Nodular pseudoangiomatous stromal hyperplasia (PASH) of the breast is rare and often indistinguishable from fibroadenoma, clinically and on aspiration biopsy smears. We report our observations in 10 patients with PASH, evaluated by fine-needle aspiration (FNA) biopsy and core biopsy. We retrospectively reviewed the cl...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.20270

    authors: Levine PH,Nimeh D,Guth AA,Cangiarella JF

    更新日期:2005-06-01 00:00:00

  • Fine-needle aspiration cytology of adenoid (acantholytic) squamous-cell carcinoma.

    abstract::Adenoid squamous-cell carcinoma (ASCCa) is an uncommon cancer which occurs most frequently in the skin of the head and neck region of elderly, sun-exposed individuals. Histologically, ASCCa is characterized by gland-like, "adenoid" cell groups with a central, detached acantholytic cellular component and an intact peri...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.2840120218

    authors: Dodd LG

    更新日期:1995-03-01 00:00:00

  • Fine needle aspiration cytology in sclerosing stromal tumor of the ovary: a series of three cases.

    abstract::Sclerosing stromal tumor (SST) of the ovary is a distinct subtype of sex cord-stromal tumor, which is derived from the ovarian stroma and, in turn, from the sex cords of the embryonic gonads. It is a benign tumor, predominantly, unilateral in nature and mostly involves the right side of the ovary. Histopathological fe...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.21639

    authors: Banik T,Gupta N,Dey P,Rajwanshi A,Dhaliwal LK

    更新日期:2012-04-01 00:00:00

  • Leiomyosarcoma metastatic to the orbit: diagnosis of fine-needle aspiration.

    abstract::A 47-yr-old man with history of metastatic low-grade rectal leiomyosarcoma presented with progressive protrusion of his left eye due to an enlarging orbital mass. The differential diagnosis included tumor metastasis or orbital infection due to an unknown infectious agent. Diagnostic fine-needle aspiration (FNA) of the...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/(sici)1097-0339(199711)17:5<369::aid-dc10>

    authors: Logrono R,Inhorn SL,Dortzbach RK,Kurtycz DF

    更新日期:1997-11-01 00:00:00

  • Diagnostic value of fine-needle aspiration in supraclavicular lymphadenopathy: a study of 106 patients and review of literature.

    abstract::The simplicity and safety of the fine-needle aspiration (FNA) make it a useful procedure for the initial evaluation of supraclavicular lymphadenopathy. We analyzed data (106 patients) in combination with the data of three recently published studies (total 301 patients) to make the observations more meaningful. The use...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章,评审

    doi:10.1002/dc.10002

    authors: Nasuti JF,Mehrotra R,Gupta PK

    更新日期:2001-12-01 00:00:00

  • Evolution of Pap testing at a community hospital: a ten year experience.

    abstract::Over a 10-year period the Pap program at our community hospital laboratory progressed from the use of 100% conventional smears to predominantly ThinPrep (TP) and then to nearly 100% SurePath (SP). During this period the annual Pap volume grew from less than 20,000 to nearly 50,000. This retrospective review focuses on...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.20607

    authors: Nance KV

    更新日期:2007-03-01 00:00:00

  • Metastatic small cell osteosarcoma to the liver: a diagnostic pitfall for fine-needle aspiration cytology.

    abstract::We report the fine-needle aspiration cytology of a case of metastatic small cell osteosarcoma to the liver with rosette formation, originating from distal femur, in a 36-year-old female. The aspirate of the liver metastases revealed a relatively monomorphic population of mitotically active small blue round cells arran...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.22894

    authors: Ali RH,Lee CH,Hayes MM

    更新日期:2014-02-01 00:00:00

  • Simultaneous cytological diagnosis of herpes simplex virus infection and primary lung cancer: report of two cases.

    abstract::Herpes simplex is an uncommon cause of lower respiratory tract infection that requires prompt diagnosis and treatment to prevent late complications. We report two cases with simultaneous herpes simplex virus infection of the lower respiratory tract and lung carcinoma. Cytology of bronchial brushing and washing fluids ...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.20921

    authors: Maounis N,Blana A,Ellina E,Chorti M,Lekkakou A,Mermigkis D,Spanakis N,Emmanouilidou A

    更新日期:2008-11-01 00:00:00

  • PREPmate automated processor: comparison of automated and manual methods of liquid-based gynecologic sample preparation.

    abstract::The development of procedures for fully automated processing of liquid-based gynecologic samples has been the focus of considerable interest to the cytology laboratory. Liquid-based collection and processing technology has been shown to improve sample adequacy, resulting in an overall improvement in quality of sample ...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.10193

    authors: Rowe LR,Marshall CJ,Bentz JS

    更新日期:2002-11-01 00:00:00

  • Extranodal NK/T-cell lymphoma, nasal type of the uterine cervix: A case report.

    abstract::We report a rare case of extranodal NK/T-cell lymphoma, nasal type of the uterine cervix that showed cytologic features mimicking cervical cancer. A 65-year-old woman presented with vaginal bleeding. Gynecological examination revealed a bulky tumor of the cervix. A conventional Papanicolaou-stained cervical smear show...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.23439

    authors: Omori M,Oishi N,Nakazawa T,Nakazawa K,Mitsumori T,Yuminamochi T,Kirito K,Hirata S

    更新日期:2016-05-01 00:00:00

  • Reproducibility of atypia of undetermined significance/follicular lesion of undetermined significance category using the bethesda system for reporting thyroid cytology when reviewing slides from different institutions: A study of interobserver variability

    abstract:BACKGROUND:The Bethesda System for Reporting Thyroid Cytopathology (TBSRTC) offers a six-tiered diagnostic scheme for thyroid Fine Needle Aspiration (FNA): Benign, Atypia of Undetermined Significance/Follicular Lesion of Undetermined Significance (AUS/FLUS), suspicious for follicular neoplasm, suspicious for malignancy...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.23681

    authors: Padmanabhan V,Marshall CB,Akdas Barkan G,Ghofrani M,Laser A,Tolgay Ocal I,David Sturgis C,Souers R,Kurtycz DF

    更新日期:2017-05-01 00:00:00

  • Pleomorphic adenoma with predominant plasmocytoid myoepithelial cells: a diagnostic pitfall in aspiration cytology. Case report and review of the literature.

    abstract::Fine-needle aspiration (FNA) biopsy of the salivary gland is a sensitive and specific diagnostic tool. However, diagnostic problems are sometimes encountered in interpreting some cases, not only in differentiating benign from malignant cases but also in the specific classification of these neoplasms. We report a case ...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章,评审

    doi:10.1002/dc.20954

    authors: Pusztaszeri M,Braunschweig R,Mihaescu A

    更新日期:2009-01-01 00:00:00

  • Pitfalls in the diagnosis of hepatic epithelioid hemangioendothelioma by FNA and needle core biopsy.

    abstract::Epithelioid hemangioendothelioma (EHE) of the liver is an extremely rare tumor that masquerades as an epithelial neoplasm and poses significant diagnostic pitfalls for the cytopathologist. This report describes a 64-year-old woman whom on computerized tomography was found to have multiple peripherally calcified hypode...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.22943

    authors: Jurczyk M,Zhu B,Laskin W,Lin X

    更新日期:2014-06-01 00:00:00

  • Diffuse large B-cell lymphoma with fibrillary matrix.

    abstract::Diffuse large B-cell lymphoma (DLBCL) with fibrillary matrix is an extremely rare variant of non-Hodgkin's lymphoma (NHL) whose pathological features are poorly known. Here, we report on our experience with such a condition arising in a 71-yr-old woman. Our findings not only demonstrate that DLBCL with fibrillary matr...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.10172

    authors: Dargent JL,Meiers I,Lespagnard L,Ma Y,Dehou MF,Verhest A

    更新日期:2002-10-01 00:00:00

  • Post-thyroid FNA testing and treatment options: a synopsis of the National Cancer Institute Thyroid Fine Needle Aspiration State of the Science Conference.

    abstract::The National Cancer Institute (NCI) sponsored the NCI Thyroid Fine Needle Aspiration (FNA) State of the Science Conference on October 22-23, 2007 in Bethesda, MD. The 2-day meeting was accompanied by a permanent informational Web site and several on-line discussion periods between May 1 and December 15, 2007 (http://t...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.20832

    authors: Layfield LJ,Abrams J,Cochand-Priollet B,Evans D,Gharib H,Greenspan F,Henry M,LiVolsi V,Merino M,Michael CW,Wang H,Wells SA

    更新日期:2008-06-01 00:00:00

  • Fine-needle aspiration of cytomegalovirus sialadenitis in a patient with acquired immunodeficiency syndrome: pitfalls of diff-quik staining.

    abstract::A case of cytomegalovirus (CMV) sialadenitis in a patient with acquired immunodeficiency syndrome (AIDS) diagnosed by fine-needle aspiration biopsy (FNAB) is reported. Although a diagnosis of CMV sialadenitis can be established on Diff-Quik-stained slides, the characteristic viral changes are the best appreciated on s...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/(sici)1097-0339(200002)22:2<101::aid-dc8>3

    authors: Santiago K,Rivera A,Cabaniss D,Dhurhar N,Moroz K

    更新日期:2000-02-01 00:00:00

  • Unusual presentation of disseminated histoplasmosis in an immunocompetent patient.

    abstract::Disseminated histoplasmosis (DH) is a systemic granulomatous disease caused by the fungus Histoplasma capsulatum. This disease occurs mainly in immunocompromised individuals with CD 4 counts less than 200 cell/µL and is rarely noted in immunocompetent person. In India, this disease has been reported from several parts...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.23742

    authors: Kumari M,Udayakumar M,Kaushal M,Madaan GB

    更新日期:2017-09-01 00:00:00

  • Primary malignant deciduoid peritoneal mesothelioma: a report of the cytohistological and immunohistochemical appearances.

    abstract::Malignant deciduoid mesothelioma (MDM) is a rare variant of epithelioid mesothelioma. This type of tumor might be associated with the asbestos exposure and carries a poor prognosis in general. MDM was first described by Nascimento et al. in 1994 in a peritoneal lesion of a young woman. And its diagnosis is frequently ...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.21400

    authors: Ustun H,Astarci HM,Sungu N,Ozdemir A,Ekinci C

    更新日期:2011-06-01 00:00:00

  • Fine-needle aspiration cytology in azoospermic males.

    abstract::Bilateral testicular fine-needle aspiration cytology (FNAC) was performed in 100 males, including 10 controls with normal fertility and 90 infertile azoospermic males. The various seminiferous tubular cells were easily identified in smears. It was possible to give a cytological diagnosis in histologic terms by analysi...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.2840070103

    authors: Rajwanshi A,Indudhara R,Goswami AK,Radhika S,Das A,Sharma SK,Vaidyanathan S,Datta BN

    更新日期:1991-01-01 00:00:00