In vitro amoebicidal activity of propamidine and pentamidine isethionate against Acanthamoeba species and toxicity to corneal tissues.

Abstract:

:Effective chemotherapy for Acanthamoeba keratitis has been hampered because of the marked resistance of the parasites to a variety of antimicrobial agents. In view of the fact that topical Brolene (propamidine isethionate) and neosporin are currently considered to be the medical treatment of choice in Europe, we sought to determine whether pentamidine may be equally effective, because the drug is more readily available to ophthalmologists in the United States. In this study, we compared the amoebicidal activity of the Brolene (commercial product), propamidine isethionate and pentamidine isethionate (Pentam) in vitro against three different species of Acanthamoeba, and the drugs' corresponding biocompatibility with rabbit corneal epithelial and endothelial cell cultures. The results indicated that there were significant species differences in drug sensitivity. Propamidine (> 1,000 micrograms/ml) was clearly less effective than pentamidine (> 125 micrograms/ml) against A. castellanii, although equivalent potency (> 250 micrograms/ml) was observed against A. polyphaga. On the other hand, propamidine (> 31.25 micrograms/ml) was slightly more effective than pentamidine (> 62.5 micrograms/ml) against A. hatchetti. Both drugs were also relatively nontoxic after short-term contact with cell cultures, even though the highest concentration of pentamidine caused low-grade injury to the superficial epithelium and reversible membrane damage to the endothelium. Steady-state levels of propamidine at effective amoebicidal concentrations, however, were much more toxic than pentamidine, which indicated that the drug has a much lower therapeutic index. Our data suggest that pentamidine may be an effective therapeutic option because of its potency and low toxicity.

journal_name

Cornea

journal_title

Cornea

authors

Alizadeh H,Silvany RE,Meyer DR,Dougherty JM,McCulley JP

subject

Has Abstract

pub_date

1997-01-01 00:00:00

pages

94-100

issue

1

eissn

0277-3740

issn

1536-4798

journal_volume

16

pub_type

杂志文章

相关文献

CORNEA文献大全
  • Selective Endothelial Removal for Peters Anomaly.

    abstract:PURPOSE:Peters anomaly is the most common cause of congenital corneal opacities. Although conservative management is often ineffective except in mild cases, surgical intervention in the form of penetrating keratoplasty is fraught with unpredictability and often has unacceptable postoperative outcomes. As such, there is...

    journal_title:Cornea

    pub_type: 杂志文章,评审

    doi:10.1097/ICO.0000000000001472

    authors: Soh YQ,Mehta JS

    更新日期:2018-03-01 00:00:00

  • Corneal transplant rejection rate and severity after endothelial keratoplasty.

    abstract:PURPOSE:To examine the corneal transplant rejection rate and the rate of graft failure subsequent to rejection in the first 2 years after endothelial keratoplasty (EK) and to compare this to background data for similar cases of penetrating keratoplasty (PK). METHODS:Anonymized data from consecutive cases of EK [deep l...

    journal_title:Cornea

    pub_type: 杂志文章,多中心研究

    doi:10.1097/ICO.0b013e31812f66e5

    authors: Allan BD,Terry MA,Price FW Jr,Price MO,Griffin NB,Claesson M

    更新日期:2007-10-01 00:00:00

  • Use of a Purpose-Built Impression Cytology Device for Gene Expression Quantification at the Ocular Surface Using Quantitative PCR and Droplet Digital PCR.

    abstract:PURPOSE:To describe an impression cytology (IC) technique using a purpose-built, sterile, EYEPRIM IC device that can be coupled with a TRIzol reagent-based RNA extraction protocol to yield sufficient RNA for gene expression analysis. METHODS:IC samples using the EYEPRIM device were collected from the bulbar conjunctiv...

    journal_title:Cornea

    pub_type: 杂志文章,随机对照试验

    doi:10.1097/ICO.0000000000001792

    authors: Ganesalingam K,Ismail S,Craig JP,Sherwin T

    更新日期:2019-01-01 00:00:00

  • The BioMask for treatment of corneal surface irregularities with excimer laser phototherapeutic keratectomy.

    abstract:PURPOSE:To assess the characteristics of BioMask as a potential masking agent for use with the excimer laser. METHOD:We addressed ablation rate, smoothness, ease of use, dioptric shift, treatment of standardized irregular topography, and ability of BioMask to induce dioptric change in vivo. RESULTS:BioMask ablates at...

    journal_title:Cornea

    pub_type: 杂志文章

    doi:10.1097/00003226-199903000-00003

    authors: Stevens SX,Bowyer BL,Sanchez-Thorin JC,Rocha G,Young DA,Rowsey JJ

    更新日期:1999-03-01 00:00:00

  • Temporary suture tarsorrhaphy after penetrating keratoplasty.

    abstract::Persistent donor corneal epithelial defects following penetrating keratoplasty may lead to graft failure. We herein describe a method for providing corneal protection using a temporary suture tarsorrhaphy. This technique is simple and completely reversible, and it allows continued observation and instillation of topic...

    journal_title:Cornea

    pub_type: 杂志文章

    doi:10.1097/00003226-199103000-00005

    authors: Koenig SB,Harris GJ

    更新日期:1991-03-01 00:00:00

  • Estimating Total Corneal Astigmatism From Anterior Corneal Data.

    abstract:PURPOSE:To determine keratometric astigmatism (KA), posterior corneal astigmatism (PCA), and total corneal astigmatism (TCA) in 951 normal eyes, to establish a model for estimating TCA from anterior corneal data, and to test this method in a new group of eyes with toric intraocular lenses (TIOLs). METHODS:We used a Pe...

    journal_title:Cornea

    pub_type: 杂志文章

    doi:10.1097/ICO.0000000000001224

    authors: Næser K,Savini G,Bregnhøj JF

    更新日期:2017-07-01 00:00:00

  • Randomized controlled trial of subconjunctival bevacizumab injection in impending recurrent pterygium: a pilot study.

    abstract:PURPOSE:To investigate the efficacy and safety of subconjunctival bevacizumab injection for the treatment of impending recurrent pterygium. METHODS:A prospective, randomized, single-masked, controlled trial was conducted in 80 patients with impending recurrent pterygium. Patients were randomized into 4 groups using ra...

    journal_title:Cornea

    pub_type: 杂志文章,随机对照试验

    doi:10.1097/ICO.0b013e3182151e0e

    authors: Lekhanont K,Patarakittam T,Thongphiew P,Suwan-apichon O,Hanutsaha P

    更新日期:2012-02-01 00:00:00

  • Cell-based approach for treatment of corneal endothelial dysfunction.

    abstract::Decompensation of the corneal endothelium causes severe visual impairments that lead to blindness. Although corneal transplantation is a well-known effective therapy for corneal endothelial dysfunction, many patients are not afforded that therapeutic opportunity owing to the worldwide shortage of donor corneas. Thus, ...

    journal_title:Cornea

    pub_type: 杂志文章,评审

    doi:10.1097/ICO.0000000000000229

    authors: Okumura N,Kinoshita S,Koizumi N

    更新日期:2014-11-01 00:00:00

  • Combination topical therapy for conjunctival primary acquired melanosis with atypia and periocular lentigo maligna.

    abstract:PURPOSE:To report the case of a patient who initially presented with primary acquired melanosis (PAM) without atypia of the conjunctiva and later developed lentigo maligna of the eyelids and PAM with atypia of the conjunctiva. We illustrate the utility of combination topical therapy with adjunctive cryotherapy to treat...

    journal_title:Cornea

    pub_type: 杂志文章

    doi:10.1097/ICO.0000000000000277

    authors: Bratton EM,Knutsen-Larson S,Durairaj VD,Gregory DG,Mellette JR

    更新日期:2015-01-01 00:00:00

  • Moderately Long-Term Safety and Efficacy of Repeat Penetrating Keratoplasty.

    abstract:PURPOSE:To investigate the moderately long-term safety and efficacy of repeat penetrating keratoplasty (PK) for treatment of a failed PK graft. METHODS:Retrospective consecutive clinical case series. Of 992 consecutive cases that underwent PK at Baptist Eye Institute, Kyoto, Japan, between April 1998 and September 201...

    journal_title:Cornea

    pub_type: 杂志文章

    doi:10.1097/ICO.0000000000001694

    authors: Kitazawa K,Wakimasu K,Kayukawa K,Yokota I,Inatomi T,Hieda O,Sotozono C,Kinoshita S

    更新日期:2018-10-01 00:00:00

  • Ofloxacin in human serum, urine, and tear film after topical application.

    abstract::We evaluated the systemic absorption of ofloxacin eyedrops in humans and their availability in the tear film. Serum, urine, and tear film concentrations of ofloxacin were measured in 30 healthy women topically treated with 0.3% ofloxacin, in both eyes, four times daily for 10 1/2 days. Serum was collected before the f...

    journal_title:Cornea

    pub_type: 杂志文章

    doi:

    authors: Borrmann L,Tang-Liu DD,Kann J,Nista J,Lin ET,Frank J

    更新日期:1992-05-01 00:00:00

  • Topical anesthetic abuse ring keratitis: report of four cases.

    abstract:PURPOSE:We present the clinicopathologic correlations of two case and two other clinical cases of topical anesthetic abuse keratopathy that were originally diagnosed as Acanthamoeba keratitis because of ring keratitis presentation and characteristic history. METHODS:Four patients who were referred to us with suspected...

    journal_title:Cornea

    pub_type: 杂志文章

    doi:

    authors: Varga JH,Rubinfeld RS,Wolf TC,Stutzman RD,Peele KA,Clifford WS,Madigan W

    更新日期:1997-07-01 00:00:00

  • The association of keratoconus with immune disorders.

    abstract:PURPOSE:To evaluate the association between keratoconus (KC) and immune disorders (IDs). METHODS:A retrospective observational case-control study of all the members in the Central District of Clalit Health Services in Israel who were diagnosed to have KC (years 2000-2007; n = 426) and 1704 age- and gender-matched cont...

    journal_title:Cornea

    pub_type: 杂志文章

    doi:10.1097/ICO.0b013e3181cb410b

    authors: Nemet AY,Vinker S,Bahar I,Kaiserman I

    更新日期:2010-11-01 00:00:00

  • Evaluation of granular corneal dystrophy with optical coherent tomography.

    abstract:OBJECTIVE:To determine the intrastromal layer of corneal deposits in granular corneal dystrophy (GCD). DESIGN:Investigative case report. METHODS:A 32-year-old woman with bilateral decreased vision because of corneal deposits was examined with cross-sectional scans using optical coherence tomography (OCT). RESULTS:Vi...

    journal_title:Cornea

    pub_type: 杂志文章

    doi:10.1097/00003226-200404000-00009

    authors: Meyer CH,Sekundo W

    更新日期:2004-04-01 00:00:00

  • Iris cavernous hemangioma associated with multiple cavernous hemangiomas in the kidney, brain, and skin.

    abstract:PURPOSE:To document the relationship of iris cavernous hemangiomas with multiorgan hemangiomas in a child. METHODS:A 7-year-old girl with kidney hemangiomas, brain hemangiomas, and skin hemangiomas developed blurred vision and darkening of the right iris. She had undergone 6 previous brain surgeries and multiple revis...

    journal_title:Cornea

    pub_type: 杂志文章

    doi:10.1097/ICO.0b013e3180303b46

    authors: Thangappan A,Shields CL,Dinowitz M,Shields JA

    更新日期:2007-05-01 00:00:00

  • Acute Idiopathic Endotheliitis Early After Corneal Cross-linking With Riboflavin and Ultraviolet-A.

    abstract:PURPOSE:To present a case of acute idiopathic endotheliitis occurred early after corneal collagen cross-linking (CXL) for progressive keratoconus, and to discuss possible risk factors, causes, and other similar complications in the literature. METHODS:A 24-year-old woman with bilateral keratoconus underwent a corneal ...

    journal_title:Cornea

    pub_type: 杂志文章

    doi:10.1097/ICO.0000000000000110

    authors: Gumus K

    更新日期:2014-06-01 00:00:00

  • Fungal keratitis caused by Scedosporium apiospermum: report of two cases and review of treatment.

    abstract:PURPOSE:We report our experience in treating two cases of Scedosporium apiospermum keratitis and provide a review of basic scientific and clinical data regarding the treatment of this visually devastating disease. METHOD:We present a case report and literature review. RESULTS:A 35-year-old woman and a 73-year-old man...

    journal_title:Cornea

    pub_type: 杂志文章,评审

    doi:10.1097/00003226-200207000-00016

    authors: Wu Z,Ying H,Yiu S,Irvine J,Smith R

    更新日期:2002-07-01 00:00:00

  • Corneal pathology in microphthalmia with linear skin defects syndrome.

    abstract:PURPOSE:To describe the histopathology of the cornea in microphthalmia with linear streaks (MLS) syndrome. METHODS:Two patients with MLS syndrome underwent penetrating keratoplasty. This study describes the histopathology and investigates immunophenotype of the corneal extracellular matrix by using keratan sulfate and...

    journal_title:Cornea

    pub_type: 杂志文章

    doi:10.1097/01.ico.0000611352.03660.a7

    authors: Kapur R,Tu EY,Toyran S,Shah P,Vangveeravong S,Lloyd WC 3rd,Edward DP

    更新日期:2008-07-01 00:00:00

  • Combined use of subconjunctival and intracorneal bevacizumab injection for corneal neovascularization.

    abstract:PURPOSE:To report on the safety and clinical use of combined subconjunctival and intracorneal bevacizumab for corneal neovascularization. METHODS:The charts of 12 consecutive patients with corneal neovascularization who received combined subconjunctival and intracorneal injections of bevacizumab (2.5 mg/0.1 mL) were r...

    journal_title:Cornea

    pub_type: 杂志文章

    doi:10.1097/ICO.0b013e31821379aa

    authors: Yeung SN,Lichtinger A,Kim P,Amiran MD,Slomovic AR

    更新日期:2011-10-01 00:00:00

  • A review of relative risks associated with four types of contact lenses.

    abstract::This paper reviews the data of Frank et al. in the light of increasing concern over the safety of contact lenses used for extended wear. Relative risks were calculated for four types of contact lenses (CLs): extended wear soft, daily wear soft, rigid gas-permeable (RGP), and polymethylmethacrylate (PMMA) lenses--worn ...

    journal_title:Cornea

    pub_type: 杂志文章

    doi:10.1097/00003226-199010001-00024

    authors: Bailey CS

    更新日期:1990-01-01 00:00:00

  • Evaluation of Human Corneal Lenticule Quality After SMILE With Different Cap Thicknesses Using Scanning Electron Microscopy.

    abstract:PURPOSE:To evaluate the surface characteristics of lenticules created by small-incision lenticule extraction (SMILE) with different cap thicknesses. METHODS:This prospective study included 20 consecutive patients who underwent bilateral SMILE. Surface regularity of the extracted corneal lenticule was analyzed using sc...

    journal_title:Cornea

    pub_type: 杂志文章

    doi:10.1097/ICO.0000000000001404

    authors: Weng S,Liu M,Yang X,Liu F,Zhou Y,Lin H,Liu Q

    更新日期:2018-01-01 00:00:00

  • Double-Line Reflection Pattern as a Simple Method to Determine Graft Orientation of Descemet Membrane Endothelial Keratoplasty.

    abstract:PURPOSE:To describe a simple finding, consisting of a double-line reflection from the graft inside the anterior chamber, that can be used to determine the correct donor tissue orientation in Descemet membrane endothelial keratoplasty: which we would like to call Berrospi's sign. METHODS:Evaluation of the presence of l...

    journal_title:Cornea

    pub_type: 杂志文章

    doi:10.1097/ICO.0000000000001889

    authors: Berrospi RD,Galvis V,Bhogal M,Tello A

    更新日期:2019-06-01 00:00:00

  • Tear film osmolarity in patients treated for glaucoma or ocular hypertension.

    abstract:PURPOSE:To evaluate tear film osmolarity in patients treated with intraocular pressure-lowering medications. METHODS:Forty patients treated for glaucoma or ocular hypertension (OHT) were consecutively recruited for the study. Each patient was asked to complete an evaluation of ocular surface disease symptoms, the Ocul...

    journal_title:Cornea

    pub_type: 杂志文章

    doi:10.1097/ICO.0b013e31823f8cb6

    authors: Labbé A,Terry O,Brasnu E,Van Went C,Baudouin C

    更新日期:2012-09-01 00:00:00

  • Modified deep anterior lamellar keratoplasty in the management of small and large epithelialized descemetoceles.

    abstract:PURPOSE:Descemetocele is a severe complication of corneal ulceration associated with a high risk of perforation. We describe a modified air-assisted deep anterior lamellar keratoplasty (DALK) technique for the surgical management of small and large epithelialized descemetoceles. METHODS:Two representative cases are pr...

    journal_title:Cornea

    pub_type: 杂志文章

    doi:10.1097/ICO.0b013e3182031c81

    authors: Gabison EE,Doan S,Catanese M,Chastang P,Ben M'hamed M,Cochereau I

    更新日期:2011-10-01 00:00:00

  • Comparison of the Center and Flex-Center Methods of Corneal Endothelial Cell Analysis in the Presence of Guttae.

    abstract:PURPOSE:To compare endothelial cell analysis by the center and flex-center methods in corneas with guttae of differing severity and to determine the minimum countable cell number for using only the flex-center method. METHODS:Forty-seven eyes with corneal guttae and 50 age-matched normal controls were enrolled in the ...

    journal_title:Cornea

    pub_type: 杂志文章

    doi:10.1097/ICO.0000000000001334

    authors: Huang J,Liu X,Tepelus TC,Nazikyan T,Chopra V,Sadda SR,Lee OL

    更新日期:2017-12-01 00:00:00

  • Morphology of the Corneal Limbus Following Standard and Accelerated Corneal Collagen Cross-Linking (9 mW/cm2) for Keratoconus.

    abstract:PURPOSE:To evaluate the morphological features of the corneal limbus as measured by in vivo confocal microscopy (IVCM) following standard and accelerated corneal collagen cross-linking (CXL) for keratoconus. METHODS:Patients with progressive keratoconus scheduled to undergo standard CXL (group 1; 31 patients, 3 mW/cm,...

    journal_title:Cornea

    pub_type: 杂志文章

    doi:10.1097/ICO.0000000000001029

    authors: Uçakhan ÖÖ,Bayraktutar B

    更新日期:2017-01-01 00:00:00

  • Vision-related impact on quality of life in an elderly patient population after corneal transplantation.

    abstract:PURPOSE:The aim of this study was to assess the vision-related quality of life (QOL) and satisfaction of elderly patients who underwent corneal transplantations. METHODS:Survey data using a modified version of the Visual Function Index were collected in February-March 2013 from 175 patients (age ≥65 years) of 414 elig...

    journal_title:Cornea

    pub_type: 杂志文章

    doi:10.1097/ICO.0000000000000021

    authors: Puri S,Robinson SB,Wang J,Sikder S

    更新日期:2014-02-01 00:00:00

  • Endothelial keratoplasty without Descemet stripping after failed penetrating keratoplasty.

    abstract:PURPOSE:To evaluate outcomes of endothelial keratoplasty (EK) without Descemet membrane stripping for treatment of failed penetrating keratoplasty (PKP). METHODS:Retrospective cohort study of all eyes that underwent EK without Descemet membrane stripping for failed PKP at one institution between May 2008 and June 2010...

    journal_title:Cornea

    pub_type: 杂志文章

    doi:10.1097/ICO.0b013e31823f8c59

    authors: Heitor de Paula F,Kamyar R,Shtein RM,Sugar A,Mian SI

    更新日期:2012-06-01 00:00:00

  • Microcomputer software applied to corneal stromal biometry.

    abstract::Here we report a new method for image analysis of the corneal stroma. To obtain the biometric characteristics of a given area, we perform three different treatments of the same image. These automated predictions closely match each parameter (length, surface area, etc.), as measured manually by a "blind investigator." ...

    journal_title:Cornea

    pub_type: 杂志文章

    doi:10.1097/00003226-199107000-00009

    authors: Karpouzas I,Durand JP,Keller N,Savoldelli M,Pouliquen Y

    更新日期:1991-07-01 00:00:00

  • Bristle Worm-Induced Keratouveitis: A Case Report.

    abstract:PURPOSE:To report keratouveitis arising from corneal penetration by a bristle worm seta (bristle). METHODS:We report on a 64-year-old woman presenting with uniocular pain, redness, reduced vision, discharge, and pustular rash of the hands after cleaning out her marine aquarium containing bristle worms and rubbing her ...

    journal_title:Cornea

    pub_type: 杂志文章

    doi:10.1097/ICO.0000000000002225

    authors: Kirk J,Gunn D,Wong N,Minchin E,Darcy K

    更新日期:2020-05-01 00:00:00