Abstract:
:Patients with small-cell carcinoma of the lung and paraneoplastic encephalomyelitis develop antibodies (anti-Hu) against HuD, a member of a family of neuronal specific RNA-binding proteins, which are also expressed by the tumor. In order to determine if the human HuD paraneoplastic antigen shares immunogenic regions between patients and several strains and species of animals, we immunized animals with HuD and several deletion constructs of this protein. All immunized animals developed high titers of anti-HuD antibodies, comparable to the antibody titers found in paraneoplastic patients. Using immunohistochemistry and Western blot analysis of human and mouse cerebral cortex, the pattern of reactivity of these antibodies could not be differentiated from the human paraneoplastic anti-Hu antibodies. None of the immunized animals developed neurologic symptoms. Western blot analysis of HuD deletion constructs demonstrated that the first and second RNA binding domains were the main immunodominant regions, and that the animal immune response was both strain and species dependent.
journal_name
J Neuroimmunoljournal_title
Journal of neuroimmunologyauthors
Sillevis Smitt P,Manley G,Dalmau J,Posner Jdoi
10.1016/s0165-5728(96)00153-1subject
Has Abstractpub_date
1996-12-01 00:00:00pages
199-206issue
1-2eissn
0165-5728issn
1872-8421pii
S0165-5728(96)00153-1journal_volume
71pub_type
杂志文章abstract::Human sera from patients with multiple sclerosis (MS) were tested using an enzyme-linked immunosorbent assay (ELISA) method on well plates coated with various dicarboxylic acid (C4 to C10) protein conjugates. Specific immunological binding was found with an azelaic acid (AzeA, C9) conjugate. The antibody titer was hig...
journal_title:Journal of neuroimmunology
pub_type: 杂志文章
doi:10.1016/0165-5728(89)90043-x
更新日期:1989-04-01 00:00:00
abstract:BACKGROUND:Increased expression of monocyte chemoattractant protein type 1 (MCP-1) is associated with HIV CNS disease. This study evaluated the temporal relationships between MCP-1 expression and HIV replication in the CNS. METHODS:MCP-1 and HIV viral load (VL) were measured in serially obtained samples of plasma and ...
journal_title:Journal of neuroimmunology
pub_type: 临床试验,杂志文章
doi:10.1016/j.jneuroim.2005.07.012
更新日期:2005-12-01 00:00:00
abstract::Transmitters and cotransmitters of the sympathetic nervous system are involved in the regulation of a variety of immune cell functions. However, it is not entirely clear what stimuli lead to the release of these molecules in immune organs. In this study, we investigated whether local ischemia can cause the parallel re...
journal_title:Journal of neuroimmunology
pub_type: 杂志文章
doi:10.1016/s0165-5728(00)00365-9
更新日期:2000-11-01 00:00:00
abstract::Aberrant activation of calpain plays a key role in the pathophysiology of several neurodegenerative disorders. Calpain is increasingly expressed in inflammatory cells in EAE and is significantly elevated in the white matter of patients with multiple sclerosis, thus calpain inhibition could be a target for therapeutic ...
journal_title:Journal of neuroimmunology
pub_type: 杂志文章
doi:10.1016/j.jneuroim.2006.08.005
更新日期:2006-11-01 00:00:00
abstract::Matrix metalloproteinases (MMPs) are believed to play a role in the pathogenesis of multiple sclerosis (MS). As methylprednisolone is the treatment of choice for a relapse, we investigated the effect of methylprednisolone on blood levels of MMPs. Baseline TIMP-1 and MMP-2 levels were lower in MS patients than in healt...
journal_title:Journal of neuroimmunology
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1016/j.jneuroim.2003.10.046
更新日期:2004-01-01 00:00:00
abstract::A significant reduction in the incidence of experimental allergic encephalomyelitis (EAE) in SJL/J mice was observed when mice were infected with lactic dehydrogenase virus (LDV) 14 days before, on day 0, or 3 days after immunization with spinal cord homogenate. These results are discussed in terms of the selective in...
journal_title:Journal of neuroimmunology
pub_type: 杂志文章
doi:10.1016/0165-5728(86)90074-3
更新日期:1986-03-01 00:00:00
abstract::Although the blood-brain barrier and blood cerebrospinal fluid barrier maintain the central nervous system (CNS) as an immunologically privileged site, T lymphocytes can migrate through unstimulated brain endothelium and epithelium to perform immune surveillance or initiate inflammation. Our prior results suggested th...
journal_title:Journal of neuroimmunology
pub_type: 杂志文章
doi:10.1016/s0165-5728(02)00172-8
更新日期:2002-08-01 00:00:00
abstract::Experimental autoimmune neuritis (EAN), an autoimmune inflammatory demyelinating disease of the peripheral nervous system, represents an animal model of the human Guillain-Barré syndrome. EAN can be induced by active immunization in several animals, including Lewis rats. In contrast, most strains of mice including the...
journal_title:Journal of neuroimmunology
pub_type: 杂志文章
doi:10.1016/s0165-5728(01)00255-7
更新日期:2001-04-02 00:00:00
abstract::Little is known about the involvement of cytokines in the pathogenesis of primary progressive (PP) multiple sclerosis (MS). We evaluated in this cross-sectional study whether IL-18, IL-12p35, IL-12p40, TNF-alpha, IFN-gamma, IL-10, IL-4, TGF-beta, IL-12Rbeta1, and IL-12Rbeta2 mRNA expression in unstimulated white blood...
journal_title:Journal of neuroimmunology
pub_type: 杂志文章
doi:10.1016/s0165-5728(01)00398-8
更新日期:2001-11-01 00:00:00
abstract::Bi-directional interactions between the central nervous system (CNS) and immune system are demonstrated by the modification of immune function using behavioral conditioning. However, the mechanisms by which the CNS achieves conditioned immunomodulation are still in question. Here, we report that the immunosuppressive ...
journal_title:Journal of neuroimmunology
pub_type: 杂志文章
doi:10.1016/s0165-5728(98)00122-2
更新日期:1998-08-01 00:00:00
abstract::Resident glial cells and invading inflammatory cells are responsible for cytokine production within the brain. Astrocytes are known to secrete a variety of cytokines upon stimulation with cytokines themselves, protein kinase C activators, bacterial or viral constituents. Astrocytes also have surface receptors for a wi...
journal_title:Journal of neuroimmunology
pub_type: 杂志文章
doi:10.1016/0165-5728(93)90286-8
更新日期:1993-08-01 00:00:00
abstract::Altered peptide ligands (APL) with increased MHC-binding properties are highly effective at inducing T cell tolerance after systemic administration in soluble form, preventing experimental autoimmune encephalomyelitis (EAE) induced with the myelin basic protein (MBP) Ac1-9 peptide. We have previously described a diver...
journal_title:Journal of neuroimmunology
pub_type: 杂志文章
doi:10.1016/j.jneuroim.2004.07.010
更新日期:2004-11-01 00:00:00
abstract::We studied the effect of acute (1 h) or chronic exposure (7 and 14 days) to delta9-tetrahydrocannabinol (delta9-THC) on immune parameters in male Swiss mice. One hour after a dose of 10 mg/kg s.c., the splenocyte proliferative response to ConA and NK activity were not inhibited, but there was a significant decrease in...
journal_title:Journal of neuroimmunology
pub_type: 杂志文章
doi:10.1016/s0165-5728(98)00177-5
更新日期:1998-12-01 00:00:00
abstract::The blood brain barrier (BBB) and the blood-cerebrospinal fluid barrier (BCB) limit the influx of immune mediators and bloodstream compounds into the central nervous system (CNS). Upon injury or infection, the integrity of these barriers is compromised and leukocyte infiltration occurs. The BCB is located in the choro...
journal_title:Journal of neuroimmunology
pub_type: 杂志文章
doi:10.1016/j.jneuroim.2007.05.005
更新日期:2007-07-01 00:00:00
abstract::Ischemic stroke is one of the leading causes of mortality and morbidity in humans. During brain ischemia and the subsequent reperfusion that occurs with stroke, the generation of the so-called "proinflammatory" prostaglandin E(2) (PGE(2)) increases significantly. Therefore, interest is growing regarding the differenti...
journal_title:Journal of neuroimmunology
pub_type: 杂志文章
doi:10.1016/j.jneuroim.2009.01.015
更新日期:2009-03-31 00:00:00
abstract::The pathogenesis of Alzheimer's disease (AD) includes the participation of the immune system. To identify antigenic targets in AD, we screened a human microvascular endothelial cell cDNA library with sera from patients with AD, and we identified rabaptin 5 (RABPT5). We detected serum IgG specific to RABPT5 in 65% of p...
journal_title:Journal of neuroimmunology
pub_type: 杂志文章
doi:10.1016/j.jneuroim.2007.09.027
更新日期:2007-12-01 00:00:00
abstract::2D-immunomics may be useful in the identification of autoantigens in neurological autoimmune diseases, but its application may be limited by denaturation of target proteins. Here we compared the capacity of a single or multiple antigens to elicit autoantibodies targeting multiple neural autoantigens by ELISA and 2D-im...
journal_title:Journal of neuroimmunology
pub_type: 杂志文章
doi:10.1016/j.jneuroim.2010.10.004
更新日期:2011-03-01 00:00:00
abstract::Tissue cysts, the hallmark of chronic Toxoplasma gondii infection, are predominantly located in the brain making clearance of the parasite difficult. Currently available anti-T. gondii drugs are ineffective on cysts and fail to prevent reactivation of latent toxoplasmosis. We examined whether abrogation of inhibitory ...
journal_title:Journal of neuroimmunology
pub_type: 杂志文章
doi:10.1016/j.jneuroim.2018.03.013
更新日期:2018-06-15 00:00:00
abstract::To determine whether TNF and TRAIL death receptors (DR), and decoy receptors (DcR), play a role in oligodendrocyte depletion in the lesions of chronic multiple sclerosis (MS), we investigated the presence and functionality of these molecules on oligodendrocytes in MS and non-MS brain tissue and on human oligodendrocyt...
journal_title:Journal of neuroimmunology
pub_type: 杂志文章
doi:10.1016/j.jneuroim.2007.05.018
更新日期:2007-08-01 00:00:00
abstract::We report here identification of novel mimicry epitopes for interphotoreceptor retinoid-binding protein (IRBP) 201-216, a candidate ocular antigen that causes experimental autoimmune uveoretinitis (EAU) in A/J mice. One mimicry epitope from Ehrlichia canis (EHC), designated EHC 44-59, induced cross-reactive T cells fo...
journal_title:Journal of neuroimmunology
pub_type: 杂志文章
doi:10.1016/j.jneuroim.2013.08.006
更新日期:2013-10-15 00:00:00
abstract::Guinea pigs inoculated with bovine spinal cord ventral horn homogenate develop a syndrome termed experimental autoimmune gray matter disease (EAGMD) characterized by extremity weakness, bulbar signs, and a loss of lower and upper motoneurons. To provide evidence for the role of autoimmune mechanisms, we have administe...
journal_title:Journal of neuroimmunology
pub_type: 杂志文章
doi:10.1016/0165-5728(91)90123-o
更新日期:1991-11-01 00:00:00
abstract::In this study the effect of long-term selective sensory vs. sympathetic denervation was examined in the rat lung. Immunoreactivities for calcitonin gene-related peptide (CGRP) on the one hand and tyrosine hydroxylase (TH) on the other, were used as indexes to assess the changes in the two nerve systems. Following long...
journal_title:Journal of neuroimmunology
pub_type: 杂志文章
doi:10.1016/0165-5728(90)90155-g
更新日期:1990-09-01 00:00:00
abstract::Intravenous immunoglobulin (IVIG) treatment of Alzheimer's disease (AD) has been encouraging. Its mechanism of action might be via anti-β-amyloid (Aβ) antibodies which facilitate Aβ clearance. However, IVIG's benefits might result from its antiviral activity, particularly against herpes simplex virus type 1 (HSV1), a ...
journal_title:Journal of neuroimmunology
pub_type: 杂志文章
doi:10.1016/j.jneuroim.2013.01.005
更新日期:2013-04-15 00:00:00
abstract::Myelin oligodendrocyte glycoprotein (MOG) is an important autoantigen in multiple sclerosis and in experimental autoimmune encephalomyelitis (EAE). We generated a T cell receptor (TCR) transgenic (Tg) mouse expressing a TCR derived from an encephalitogenic T cell clone specific for MOG(35-55). This mouse failed to dev...
journal_title:Journal of neuroimmunology
pub_type: 杂志文章
doi:10.1016/j.jneuroim.2003.12.007
更新日期:2004-04-01 00:00:00
abstract::An electroimmunoblotting technique was used with a monoclonal antibody to myelin basic protein (MBP) to assess demyelination in 3 defined regions of the spinal cord in rats with acute experimental autoimmune encephalomyelitis (EAE). A slight loss in MBP was detected only in the sacrococcygeal region of the spinal cord...
journal_title:Journal of neuroimmunology
pub_type: 杂志文章
doi:10.1016/s0165-5728(85)80035-7
更新日期:1985-10-01 00:00:00
abstract::This study describes local immune responses in cerebral ischemia induced by permanent occlusion of the middle cerebral artery (MCAO) in the rat. The temporal and spatial pattern of leukocyte infiltration was characterized immunocytochemically using monoclonal antibodies against CD5, a pan T cell marker, against CD4 an...
journal_title:Journal of neuroimmunology
pub_type: 杂志文章
doi:10.1016/0165-5728(94)90010-8
更新日期:1994-12-01 00:00:00
abstract::To elucidate the factor(s) accelerating the autoimmune disease processes, we induced two types of experimental autoimmune encephalomyelitis (EAE), severe and very mild, in F344 rats by immunization with myelin basic protein (MBP) plus pertussis toxin (PT) (PT+) or with MBP alone (PT-) and compared the differences betw...
journal_title:Journal of neuroimmunology
pub_type: 杂志文章
doi:10.1016/s0165-5728(99)00242-8
更新日期:2000-04-03 00:00:00
abstract::T lymphocytes are major contributors to post-ischemic neuroinflammation following ischemic stroke. However, the mode of postischemic T cell activation remains a controversial issue. Therefore, this study aimed to directly investigate antigen-dependent clonal T cell expansion after experimental brain ischemia using spe...
journal_title:Journal of neuroimmunology
pub_type: 杂志文章
doi:10.1016/j.jneuroim.2013.01.013
更新日期:2013-04-15 00:00:00
abstract::Primary angiitis of the central nervous system (PACNS) is a rare and heterogeneous inflammatory disease of the CNS vasculature with poorly understood pathophysiology. Comprehensive immune-cell phenotyping revealed increased frequencies of leukocytes in the cerebrospinal fluid (CSF) of PACNS patients compared to patien...
journal_title:Journal of neuroimmunology
pub_type: 杂志文章
doi:10.1016/j.jneuroim.2018.06.004
更新日期:2018-08-15 00:00:00
abstract::Adult Lewis (LEW) rats are highly susceptible to experimental autoimmune encephalomyelitis (EAE), induced actively by immunization with guinea pig (GP) myelin basic protein (MBP) in complete Freund's adjuvant or adoptively transferred with activated T lymphocytes reactive to GP MBP peptide 68-88. Once LEW rats recover...
journal_title:Journal of neuroimmunology
pub_type: 杂志文章
doi:10.1016/s0165-5728(97)00176-8
更新日期:1998-01-01 00:00:00