Abstract:
:Since the introduction of hematopoietic growth factors, the collection of mobilized stem cells via leukapheresis has widely replaced the harvest of bone marrow in both autologous and allogeneic transplantation settings. We investigated the frequency and the extent of anticoagulant-induced electrolyte changes and the cell-separation-related loss of hemoglobin and platelets. In our study a total of 200 leukaphereses were performed on 60 patients with hematological malignancies. The electrolytes (calcium and potassium) were determined photometrically pre- and post-apheresis. Blood counts were analyzed to calculate the relative decline in hemoglobin and platelet counts. Stem cells were collected by processing a mean total blood volume of 11.6+/-3.9 L with a citrate consumption of 1,345+/-126 mL. More than 50% of all patients needed replacement therapy of either potassium or calcium. In non-substituted patients the initial serum potassium concentration dropped by 11.3+/-7.0% to 3.25+/-0.33 mmol/L post apheresis. In 21% of non-substituted patients, clinical relevant hypokalaemia was observed with levels < 3 mmol/L. The mean citrate-induced reduction of the total calcium was 5.5+/-6.0%. In addition the relative loss of hemoglobin and platelet counts amounted to 10.7+/-5.2% and 24.2+/-12.5%, respectively. In addition to the well-documented citrate-induced hypocalcaemia, we observed a considerable reduction in serum potassium during stem cell apheresis. This can result in a clinically relevant, substitution requiring hypokalaemia. The modest decline in hemoglobin and platelet counts suggested that levels of >9 g/dl (Hb) and platelets >30 x 10(9)/L are sufficient for a safe standard leukapheresis.
journal_name
J Clin Apherjournal_title
Journal of clinical apheresisauthors
Schlenke P,Frohn C,Steinhardt MM,Kirchner H,Klüter Hdoi
10.1002/1098-1101(2000)15:4<230::aid-jca3>3.0.co;2subject
Has Abstractpub_date
2000-01-01 00:00:00pages
230-5issue
4eissn
0733-2459issn
1098-1101pii
10.1002/1098-1101(2000)15:4<230::AID-JCA3>3.0.CO;2journal_volume
15pub_type
杂志文章abstract::The cell separator MCS-3P is an apheresis system offering the flexibility to collect standardized red blood cells, plasma, and/or platelets from one donor. Two different programs were used for the red cell apheresis--RBCP (collection of one unit of red cells and two units of plasma) and RBCPS (one unit of red cells an...
journal_title:Journal of clinical apheresis
pub_type: 杂志文章
doi:10.1002/jca.2920090308
更新日期:1994-01-01 00:00:00
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journal_title:Journal of clinical apheresis
pub_type: 历史文章,杂志文章,评审
doi:10.1002/jca.20089
更新日期:2006-04-01 00:00:00
abstract:BACKGROUND:The ability to cryopreserve a portion of the cells treated during extracorporeal photopheresis (ECP) would improve therapy logistics, particularly for pediatric patients, by allowing multiple therapeutic doses to be collected from a single apheresis session. However, the effect of cryopreservation on ECP-tre...
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abstract::Two patients suffering from allergic bronchial asthma who showed no improvement despite six and four weeks, respectively, of drug therapy were successfully treated with therapeutic plasma exchange. The first patient had no attacks over a period of five months, and the other patient had none for over one year. Although...
journal_title:Journal of clinical apheresis
pub_type: 杂志文章
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abstract::Until recently, the collection of peripheral blood progenitor cells (PBPC) has been semi-automated by using the COBE Spectra, with the operator manually maintaining the position of the white cells being collected. The COBE Spectra Version 6.0 apheresis device offers the user an automated program for the collection of ...
journal_title:Journal of clinical apheresis
pub_type: 临床试验,杂志文章
doi:10.1002/(sici)1098-1101(1999)14:1<26::aid-jca5>3.0
更新日期:1999-01-01 00:00:00
abstract::The purpose of this study is to determine whether immunoadsorption treatment using a dextran sulfate (DS) column can remove high-avidity anti-double-stranded DNA (anti-dsDNA) antibody from the blood of patients with systemic lupus erythematosus (SLE). Before and after each immunoadsorption therapy routine, titers of t...
journal_title:Journal of clinical apheresis
pub_type: 杂志文章
doi:10.1002/(SICI)1098-1101(1996)11:1<30::AID-JCA7>3.0
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journal_title:Journal of clinical apheresis
pub_type: 杂志文章,评审
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更新日期:2019-08-01 00:00:00
abstract::The influence of 6 weeks of cyclosporin A (CYA), followed by a further 6 weeks of this agent in combination with plasma exchange (PE), was defined on panel reactivity and titre of preformed cytotoxic antibodies in stable patients on haemodialysis awaiting renal transplantation. Nine individuals with antibodies to 30% ...
journal_title:Journal of clinical apheresis
pub_type: 杂志文章
doi:10.1002/jca.2920060107
更新日期:1991-01-01 00:00:00
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更新日期:2006-12-01 00:00:00
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journal_title:Journal of clinical apheresis
pub_type: 杂志文章
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更新日期:2015-12-01 00:00:00
abstract::A patient with a hyper-IgE syndrome was treated with 60 plasmaphereses over a period of 2 years in conjunction with cytotoxic immunosuppressive drug therapy. During this time her severe dermatitis of 8 years' duration became almost completely inactive, and her circulating IgE level was reduced by 73%. An elevated pret...
journal_title:Journal of clinical apheresis
pub_type: 杂志文章
doi:10.1002/jca.2920040104
更新日期:1988-01-01 00:00:00
abstract::Staphylococcal protein A efficiently binds immunoglobulins and circulating immune complexes (CIC) and provides an effective medium to remove immunoglobulins and CICs from plasma while sparing albumin and most coagulation proteins. Although it activates the complement system its clinical use abrogates the need for plas...
journal_title:Journal of clinical apheresis
pub_type: 杂志文章,评审
doi:10.1002/jca.2920090109
更新日期:1994-01-01 00:00:00
abstract::Idiopathic inflammatory demyelinating diseases (IIDDs) of the central nervous system, of which multiple sclerosis is the prototype, represent a family of monophasic, recurrent or progressive diseases with overlapping clinical and pathological manifestations. While most patients recover spontaneously or following a bri...
journal_title:Journal of clinical apheresis
pub_type: 杂志文章,评审
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更新日期:1999-01-01 00:00:00
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journal_title:Journal of clinical apheresis
pub_type: 杂志文章
doi:10.1002/jca.2920070408
更新日期:1992-01-01 00:00:00
abstract::Thrombopoietin (c-Mpl ligand) is the hematopoietic growth factor that is responsible for regulating the production of platelets from bone marrow megakaryocytes. This approximately 90 kd protein has recently been isolated and is comprised of an erythropoietin domain that is approximately 50% homologous to erythropoieti...
journal_title:Journal of clinical apheresis
pub_type: 杂志文章,评审
doi:10.1002/(SICI)1098-1101(1996)11:3<149::AID-JCA6>3.
更新日期:1996-01-01 00:00:00
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journal_title:Journal of clinical apheresis
pub_type: 杂志文章
doi:10.1002/jca.21645
更新日期:2018-10-01 00:00:00
abstract::Because the administration of hematopoietic growth factors and the use of stem cell support often fails to alleviate the neutropenic phase induced by cytotoxic drugs, several investigators have attempted to expand ex vivo hematopoietic progenitors for clinical use. These attempts have clearly shown that the cultured c...
journal_title:Journal of clinical apheresis
pub_type: 杂志文章
doi:10.1002/jca.10012
更新日期:2002-01-01 00:00:00
abstract:OBJECTIVE:Effects of therapeutic plasma exchange (TPE) on immune cells and their cytokine production in MS, are unknown. Since interleukine-6 and tumor growth factor-β have critical roles in MS immunopathogenesis, the impacts of TPE on the expression of these cytokines and their receptors on the surface of CD4+ T lymph...
journal_title:Journal of clinical apheresis
pub_type: 杂志文章
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更新日期:2020-04-01 00:00:00
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journal_title:Journal of clinical apheresis
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journal_title:Journal of clinical apheresis
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journal_title:Journal of clinical apheresis
pub_type: 杂志文章,随机对照试验
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更新日期:2014-04-01 00:00:00
abstract::In the United States, therapeutic plasma exchange (TPE) is both performed and requested by a wide range of services, often on an empiric basis (before a diagnosis is established). Whether empiric therapy is beneficial has not been established. Patients were identified from an electronic procedure log that included tho...
journal_title:Journal of clinical apheresis
pub_type: 杂志文章
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更新日期:2005-12-01 00:00:00
abstract::An improved method for intermittent-flow erythrocytapheresis in patients with sickle cell disease is reported. The method, a modification of the standard red cell exchange procedure for the Haemonetics 30S unit, dilutes with physiologic saline the patient's blood as it flows from the draw line and before it reaches th...
journal_title:Journal of clinical apheresis
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更新日期:1986-01-01 00:00:00
abstract::The process of publishing original work in a peer review journal is not complete at the point where the manuscript is first submitted. The journal editors will submit the manuscript to peer review whereby outside experts are asked to vet the manuscript for scientific merit, originality and quality. Reviewers' comments...
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pub_type: 杂志文章,评审
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更新日期:2020-08-07 00:00:00
abstract::Modern techniques of mechanical hemapheresis have made it possible to selectively remove vast quantities of lymphocytes and plasma immunoglobulins, and the concentration of these substances in donor blood can fall below the normal range. It is feared that this may lead to immunosuppression; a condition associated in s...
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更新日期:1984-01-01 00:00:00
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journal_title:Journal of clinical apheresis
pub_type: 临床试验,杂志文章,随机对照试验
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abstract::Transfusion-acquired babesiosis can be an asymptomatic or self-limited febrile hemolytic illness in a healthy host. A persistent, relapsing, and/or fulminant course with the development of life-threatening complications may be seen in immunocompromised or splenectomized patients. As in malaria, erythrocyte parasitemia...
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更新日期:2010-01-01 00:00:00
abstract::Plerixafor in combination with granulocyte-colony stimulating factor (G-CSF) is approved for autologous stem cell mobilization in poor mobilizing patients with multiple myeloma or malignant lymphoma. The purpose of this study was to evaluate efficacy and safety of plerixafor in an immediate rescue approach, administra...
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更新日期:2017-08-01 00:00:00