Confirmation of the Syva MicroTrak enzyme immunoassay for chlamydia trachomatis by Syva Direct Fluorescent Antibody Test.

Abstract:

BACKGROUND AND OBJECTIVES:The Syva Micro Trak enzyme immunoassay (EIA) is used widely for screening women infected with Chlamydia trachomatis. Confirmatory tests used in conjunction with EIA screening have shown that false-positive results are common. GOALS:To evaluate the specificity of the Syva MicroTrak EIA by confirmation of positive specimens with the Syva Direct Fluorescent Specimen Test. STUDY DESIGN:Of 6,039 endocervical specimens collected from women attending Colorado family planning clinics, 328 positive EIA results (5.4%) were obtained by Syva MicroTrak EIA. A random subset of 136 positive specimens was tested by Syva Direct Specimen Test. Twenty of 136 specimens (14.7%) negative by Syva Direct Specimen testing were also tested by Syva blocking antibody tests (9 of 20 positive, 45%) and Roche Amplicor polymerase chain reaction (PCR; 6 of 20 positive, 30%). Of 20 specimens positive by Syva MicroTrak EIA and negative by Syva Direct Specimen Test, 11 (55%) were also negative by blocking antibody and PCR, including three specimens with initial EIA sample-to-cutoff ratios greater than 2. CONCLUSIONS:Confirmatory testing of Syva MicroTrak EIA positive specimens with Syva Direct Specimen Test showed that 14.7% were false positive. Coupling the Syva Direct Specimen test with either blocking antibody or PCR reduces the rate of false-positive results to 8%.

journal_name

Sex Transm Dis

authors

Beebe JL,Masters H,Jungkind D,Heltzel DM,Weinberg A

doi

10.1097/00007435-199611000-00005

subject

Has Abstract

pub_date

1996-11-01 00:00:00

pages

465-70

issue

6

eissn

0148-5717

issn

1537-4521

journal_volume

23

pub_type

临床试验,杂志文章,随机对照试验
  • Neurocognitive impairment in human immunodeficiency virus infection is correlated with sexually transmitted disease history.

    abstract:BACKGROUND AND OBJECTIVES:Neurocognitive impairment is common in human immunodeficiency virus (HIV)-infected subjects. The relationship of sexually transmitted diseases to neurocognitive changes is unknown. GOAL:To establish whether HIV-infected patients with a history of syphilis or gonorrhea have a higher rate of ne...

    journal_title:Sexually transmitted diseases

    pub_type: 杂志文章

    doi:10.1097/00007435-199708000-00003

    authors: Wallace MR,Heaton RK,McCutchan JA,Malone JL,Velin R,Nelson J,Miller LK,Weiss PJ,Oldfield EC 3rd,Grant I

    更新日期:1997-08-01 00:00:00

  • Willingness to Disclose Sexually Transmitted Infection Status to Sex Partners Among College-Aged Men in the United States.

    abstract::Disclosure of sexually transmitted infections (STIs) to sexual partners is critical to the prevention, treatment, and control of STIs. We examine personal intra and interpersonal influences on willingness to disclose STI status among college-aged men. Participants (n = 1064) were aged 17 to 24 years and recruited from...

    journal_title:Sexually transmitted diseases

    pub_type: 杂志文章

    doi:10.1097/OLQ.0000000000000420

    authors: Pfeiffer EJ,McGregor KA,Van Der Pol B,Hardy Hansen C,Ott MA

    更新日期:2016-03-01 00:00:00

  • Context-Appropriate Interventions to Prevent Syphilis: A Narrative Review.

    abstract:BACKGROUND:The success of an intervention to prevent syphilis will depend on the context and the goal of the intervention. To help programs choose interventions, we reviewed major changes in context and types of interventions that may be effective. METHODS:We reviewed the literature on the changing context of syphilis...

    journal_title:Sexually transmitted diseases

    pub_type: 杂志文章,评审

    doi:10.1097/OLQ.0000000000000804

    authors: Peterman TA,Cha S

    更新日期:2018-09-01 00:00:00

  • Identifying opportunities for chlamydia screening among American Indian women.

    abstract::Medical record review was used to identify missed opportunities for chlamydia screening among 103 American Indian/Alaska Native women. Of these, 69% had received a pregnancy test and 74% had received a urine test in the previous 12 months. Chlamydia screening may increase if linked to the other routine clinical testin...

    journal_title:Sexually transmitted diseases

    pub_type: 杂志文章

    doi:10.1097/OLQ.0b013e318222f126

    authors: Taylor MM,Reilley B,Tulloch S,Winscott M,Dunnigan A,Russell M,Redd JT

    更新日期:2011-10-01 00:00:00

  • CXCL13 as a cerebrospinal fluid marker for neurosyphilis in HIV-infected patients with syphilis.

    abstract:BACKGROUND:Asymptomatic neurosyphilis is more difficult to diagnose in human immunodeficiency virus (HIV)-infected patients because HIV itself can cause cerebrospinal fluid (CSF) pleocytosis. The proportion of CSF lymphocytes that are B cells is elevated in neurosyphilis, suggesting that the CSF concentration of the B ...

    journal_title:Sexually transmitted diseases

    pub_type: 杂志文章

    doi:10.1097/OLQ.0b013e3181d877a1

    authors: Marra CM,Tantalo LC,Sahi SK,Maxwell CL,Lukehart SA

    更新日期:2010-05-01 00:00:00

  • Failure of serosorting to protect African American men who have sex with men from HIV infection.

    abstract:BACKGROUND:Serosorting is the practice of choosing sex partners or selectively using condoms based on a sex partner's perceived HIV status. The extent to which serosorting protects African American (AA) and Hispanic men who have sex with men (MSM) is unknown. METHODS:We analyzed data collected from MSM sexually transm...

    journal_title:Sexually transmitted diseases

    pub_type: 杂志文章

    doi:10.1097/OLQ.0b013e31825727cb

    authors: Golden MR,Dombrowski JC,Kerani RP,Stekler JD

    更新日期:2012-09-01 00:00:00

  • Risk factors for incident and recurrent condylomata acuminata among men. A population-based study.

    abstract:BACKGROUND AND OBJECTIVES:The rapid increase in the number of physician office visits for condylomata acuminata and the association of human papillomavirus and cancer has prompted renewed interest in the epidemiology of this sexually-transmitted disease. Few epidemiologic studies have examined what risk factors are ass...

    journal_title:Sexually transmitted diseases

    pub_type: 杂志文章

    doi:10.1097/00007435-199807000-00002

    authors: Van Den Eeden SK,Habel LA,Sherman KJ,McKnight B,Stergachis A,Daling JR

    更新日期:1998-07-01 00:00:00

  • Diagnosis of male Chlamydia trachomatis urethritis by polymerase chain reaction.

    abstract:BACKGROUND AND OBJECTIVES:A quick and highly sensitive diagnostic test would be valuable in the diagnosis of Chlamydia trachomatis. GOAL OF THIS STUDY:We compared a new polymerase chain reaction (PCR) technique to Chlamydiazyme (Abbott, North Chicago, IL) in the detection of male chlamydial urethritis, and evaluated t...

    journal_title:Sexually transmitted diseases

    pub_type: 临床试验,杂志文章

    doi:10.1097/00007435-199409000-00004

    authors: Wiesenfeld HC,Uhrin M,Dixon BW,Sweet RL

    更新日期:1994-09-01 00:00:00

  • Prevalence trends of the new variant of Chlamydia trachomatis in four counties of Sweden in 2007-2011.

    abstract::A new variant of Chlamydia trachomatis (nvCT) was discovered in Sweden in 2006, and it could not be detected by diagnostic systems from Abbott and Roche, whereas the third system used, from Becton Dickinson (BD), detects nvCT. We analyzed 3648 samples from 2 counties that used Roche and 2 counties that used BD methods...

    journal_title:Sexually transmitted diseases

    pub_type: 杂志文章

    doi:10.1097/OLQ.0b013e3182593be7

    authors: Herrmann B,Eden D,Hadad R,Christerson L,Loré B,Osterlund A,Larsson I,Sylvan S,Fredlund H,Unemo M

    更新日期:2012-08-01 00:00:00

  • Urethritis caused by Neisseria meningitidis group B: a case report.

    abstract::We describe a case of acute urethritis caused by Neisseria meningitidis group B in a heterosexual man. The symptoms started five days after intercourse that included fellatio with a casual female partner. No other urethral pathogens could be isolated. After intramuscular administration of penicillin G, clinical signs ...

    journal_title:Sexually transmitted diseases

    pub_type: 杂志文章

    doi:10.1097/00007435-198807000-00007

    authors: Hartmann AA,Elsner P

    更新日期:1988-07-01 00:00:00

  • Papillomavirus infection among abortion applicants and patients at a sexually transmitted disease clinic.

    abstract::The base-line prevalence of human papillomavirus (HPV) infection among applicants for first trimester induced abortion and among a sexually transmitted disease (STD) clinic population with macroscopically visible condyloma is investigated. Cervical cells were collected from 505 women applying for induced abortion. Cel...

    journal_title:Sexually transmitted diseases

    pub_type: 杂志文章

    doi:

    authors: Csángó PA,Skuland J,Nilsen A,Pedersen BS,Jagars G

    更新日期:1992-05-01 00:00:00

  • Neighborhoods at risk: estimating risk of higher Neisseria gonorrhoeae incidence among women at the census tract level.

    abstract:BACKGROUND:The association between area-based social factors and sexually transmitted diseases has been demonstrated in numerous studies. Such associations have not previously been explored for their potential to quantify likelihood of higher transmission of gonorrhea in small geographic areas. METHODS:Aggregate censu...

    journal_title:Sexually transmitted diseases

    pub_type: 杂志文章,多中心研究

    doi:10.1097/OLQ.0000000000000195

    authors: Stenger MR,Samuel MC,Anschuetz GL,Pugsley R,Eaglin M,Klingler E,Reed M,Schumacher CM,Simon J,Weinstock H

    更新日期:2014-11-01 00:00:00

  • Effect of multiple freeze and thaw cycles on the sensitivity of IgG and IgM immunoglobulins in the sera of patients with syphilis.

    abstract::We describe the effects of multiple freeze and thaw cycles on the sensitivity of the immunoglobulins IgG and IgM measured by enzyme-linked immunoassays in the sera of patients with syphilis. Stored frozen sera can withstand repeated freezing and thawing cycles with a minimal detrimental effect on the sensitivity of th...

    journal_title:Sexually transmitted diseases

    pub_type: 杂志文章

    doi:10.1097/OLQ.0000000000000036

    authors: Castro AR,Jost HA

    更新日期:2013-11-01 00:00:00

  • Serum concentration of protein CA-125 in women with pelvic inflammatory disease.

    abstract::The concentration of the protein CA-125 in serum was determined in women with a normal pelvis visualized at laparoscopy (control group, n = 15) and compared with serum levels of this protein in women with pelvic inflammatory disease (PID) (n = 14). Mean serum concentrations of CA-125 were similar in the two groups: 17...

    journal_title:Sexually transmitted diseases

    pub_type: 杂志文章

    doi:10.1097/00007435-198807000-00005

    authors: Brumsted JR,Mead PB,Gibson M

    更新日期:1988-07-01 00:00:00

  • Condoms for men, not women. Results of brief promotion programs.

    abstract::Three different brief intervention programs to promote condom use were tested among patients in inner-city sexually transmitted disease (STD) clinics. The first, "Condom Skills," focused on teaching mechanical aspects of how to use a condom. The second, "Social Influences," emphasized how to negotiate condom use with ...

    journal_title:Sexually transmitted diseases

    pub_type: 杂志文章

    doi:10.1097/00007435-199209000-00002

    authors: Cohen DA,Dent C,MacKinnon D,Hahn G

    更新日期:1992-09-01 00:00:00

  • Comparison of cefotetan plus doxycycline with cefoxitin plus doxycycline in the inpatient treatment of acute salpingitis.

    abstract::Pelvic inflammatory disease (PID), one of the major sequelae of the current epidemic of sexually transmitted diseases, has been shown to be a polymicrobial infection. This study compares the efficacy and safety of two broad-spectrum combination drug regimens, cefotetan with doxycycline and cefoxitin with doxycycline, ...

    journal_title:Sexually transmitted diseases

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:10.1097/00007435-199118020-00012

    authors: Walker CK,Landers DV,Ohm-Smith MJ,Robbie MO,Luft J,Schachter J,Sweet RL

    更新日期:1991-04-01 00:00:00

  • Serologic groups of Neisseria gonorrhoeae recovered from patients in Nashville, Tennessee, 1971--1976.

    abstract::The objective of this study was to develop and apply a serologic method for identification of different strains of Neisseria gonorrhoeae. Several procedures for preparation of cell-free extracts were tried; the most satisfactory was that with phosphate-buffered saline solution. Both capillary tube and immunodiffusion ...

    journal_title:Sexually transmitted diseases

    pub_type: 杂志文章

    doi:10.1097/00007435-198004000-00002

    authors: Quinn RW,Lowry PN,Hellerqvist CG

    更新日期:1980-04-01 00:00:00

  • The social context of syphilis persistence in the southeastern United States.

    abstract::In light of the racial-ethnic and poverty composition of the United States population and the distribution of syphilis morbidity across population subgroups, it is important to determine how race-ethnicity and poverty jointly and independently affect transmission dynamics of syphilis and other sexually transmitted dis...

    journal_title:Sexually transmitted diseases

    pub_type: 杂志文章

    doi:10.1097/00007435-199601000-00005

    authors: Aral SO

    更新日期:1996-01-01 00:00:00

  • Prevalence and correlates of herpes simplex virus type 2 infection in a population-based survey of young women in low-income neighborhoods of Northern California. The Young Women's Survey Team.

    abstract:BACKGROUND:Herpes simplex virus type 2 (HSV-2) is one of the most common sexually transmitted infections and may enhance transmission of HIV. However, population-based estimates of HSV-2 prevalence and correlates of infection are rare. GOALS:To obtain population-based estimates of HSV-2 prevalence and to identify demo...

    journal_title:Sexually transmitted diseases

    pub_type: 杂志文章

    doi:10.1097/00007435-200008000-00004

    authors: Buchacz K,McFarland W,Hernandez M,Klausner JD,Page-Shafer K,Padian N,Molitor F,Ruiz JD,Bolan G,Morrow S,Katz MH

    更新日期:2000-08-01 00:00:00

  • Response of guinea pigs to intravaginal inoculation with guinea pig cytomegalovirus.

    abstract::Female guinea pigs were inoculated intravaginally with guinea pig cytomegalovirus (GPCMV) propagated either in guinea pig embryo fibroblast cultures (GPEF) or salivary glands. The incidence of infection was higher with GPEF virus. Rare instances of isolation of GPCMV from cervical swabs 9-48 hr after inoculation was a...

    journal_title:Sexually transmitted diseases

    pub_type: 杂志文章

    doi:10.1097/00007435-198901000-00009

    authors: Barron AL,Menna JH,Moses EB,Rank RG,Ryu H,White HJ

    更新日期:1989-01-01 00:00:00

  • Anal lymphogranuloma venereum infection screening with IgA anti-Chlamydia trachomatis-specific major outer membrane protein serology.

    abstract:BACKGROUND:Anal lymphogranuloma venereum (LGV) infections, caused by Chlamydia trachomatis biovar L (Ct+/LGV+), are endemic among men who have sex with men (MSM). Anal non-LGV biovar Ct infections (Ct+/LGV-) can be eradicated with 1 week doxycycline, whereas Ct+/LGV+ infections require 3-week doxycycline. To differenti...

    journal_title:Sexually transmitted diseases

    pub_type: 杂志文章

    doi:10.1097/OLQ.0b013e3181e50671

    authors: de Vries HJ,Smelov V,Ouburg S,Pleijster J,Geskus RB,Speksnijder AG,Fennema JS,Morré SA

    更新日期:2010-12-01 00:00:00

  • Lymphogranuloma venereum in a pregnant woman.

    abstract::Lymphogranuloma venereum was diagnosed postpartum in a young black woman, who was a drug abuser. Chlamydia trachomatis was isolated from aspirate of a left inguinal mass, and the patient was also seropositive for human immunodeficiency virus. During hospitalization she was treated with ampicillin, gentamicin, and doxy...

    journal_title:Sexually transmitted diseases

    pub_type: 杂志文章

    doi:10.1097/00007435-198807000-00006

    authors: Heaton S,Hammerschlag MR,Roblin PM,Di Pasquale RC

    更新日期:1988-07-01 00:00:00

  • The need and plan for global elimination of congenital syphilis.

    abstract::In every society, congenital syphilis (CS) has significant medical, economic, societal and emotional burdens; these are poorly characterized but high. Inexplicably, the elimination of CS has failed to attract international attention. Yet, the cornerstones of programs to do this universally are in place, the recent dev...

    journal_title:Sexually transmitted diseases

    pub_type: 杂志文章,评审

    doi:10.1097/01.olq.0000261456.09797.1b

    authors: Schmid GP,Stoner BP,Hawkes S,Broutet N

    更新日期:2007-07-01 00:00:00

  • Clinical and virologic response to episodic acyclovir for genital ulcers among HIV-1 seronegative, herpes simplex virus type 2 seropositive African women: a randomized, placebo-controlled trial.

    abstract::In a randomized trial among African women with recurrent genital herpes, episodic acyclovir therapy resulted in modestly greater likelihood of lesion healing (hazard ratio [HR] = 1.48, P = 0.098; mean, 5.1 vs. 6.0 days) and cessation of herpes simplex virus shedding (HR = 1.88, P = 0.008; mean, 3.0 vs. 5.0 days) compa...

    journal_title:Sexually transmitted diseases

    pub_type: 杂志文章,随机对照试验

    doi:10.1097/OLQ.0b013e31823b50c6

    authors: Baeten JM,Reid SE,Delany-Moretlwe S,Hughes JP,Wang RS,Wilcox E,Limbada M,Akpomiemie G,Corey L,Wald A,Celum C

    更新日期:2012-01-01 00:00:00

  • Sex in a lifetime: Sexual behaviors in the United States by lifetime number of sex partners, 2006-2010.

    abstract::Using data from the 2006-2010 National Survey of Family Growth, we found that lifetime number of vaginal sex partners is positively associated with the number of sex partners in the past 12 months and negatively associated with age at first vaginal intercourse among 15- to 44-year-old male and female respondents. ...

    journal_title:Sexually transmitted diseases

    pub_type: 杂志文章

    doi:10.1097/OLQ.0000000000000132

    authors: Haderxhanaj LT,Leichliter JS,Aral SO,Chesson HW

    更新日期:2014-06-01 00:00:00

  • Déjà vu? A Comparison of Syphilis Outbreaks in Houston, Texas.

    abstract:BACKGROUND:After reaching an all-time low in 2000, syphilis incidence in the United States has increased as the burden shifted from heterosexuals to men who have sex with men (MSM). Houston, Texas, experienced 2 outbreaks of syphilis during this transformation in trends. Further evaluation is necessary to determine if ...

    journal_title:Sexually transmitted diseases

    pub_type: 历史文章,杂志文章

    doi:10.1097/OLQ.0000000000000488

    authors: Hallmark CJ,Hill MJ,Luswata C,Watkins KL,Thornton L,McNeese M,Ross MW

    更新日期:2016-09-01 00:00:00

  • Azithromycin treatment failure among primary and secondary syphilis patients in Shanghai.

    abstract:BACKGROUND:Azithromycin has been used to treat primary and secondary syphilis and as prophylaxis for sexual partners. We evaluated syphilis treatment failure in patients who received azithromycin therapy. METHODS:Patients who did not respond to azithromycin therapy were referred to Shanghai Skin Disease and sexually t...

    journal_title:Sexually transmitted diseases

    pub_type: 杂志文章

    doi:10.1097/OLQ.0b013e3181e2c753

    authors: Zhou P,Li K,Lu H,Qian Y,Gu X,Gong W,Tucker JD,Cohen MS

    更新日期:2010-11-01 00:00:00

  • Plasmid patterns and in-vitro susceptibility of penicillinase-producing Neisseria gonorrhoeae with emphasis on thiamphenicol: results of surveillance in The Netherlands.

    abstract::Susceptibility to thiamphenicol was tested, and plasmids were characterized for 1925 strains of penicillinase-producing Neisseria gonorrhoeae (PPNG) isolated in The Netherlands in 1981 and 1982. Of the 1925 isolates, 771 harbored the so-called "Asia" (4.5-megadalton) resistance plasmid and 1154 had the so called "Afri...

    journal_title:Sexually transmitted diseases

    pub_type: 杂志文章

    doi:10.1097/00007435-198410001-00011

    authors: van Klingeren B,van Wijngaarden LJ,Dessens-Kroon M

    更新日期:1984-10-01 00:00:00

  • Acceptability of formulations and application methods for vaginal microbicides among drug-involved women: results of product trials in three cities.

    abstract:BACKGROUND AND OBJECTIVES:Female-controlled methods of HIV prevention, such as vaginal microbicides, are urgently needed, particularly among drug-involved women. Acceptability research is critical to product development. GOAL:To assess the acceptability of forms and application methods for future microbicides. DESIGN...

    journal_title:Sexually transmitted diseases

    pub_type: 杂志文章

    doi:10.1097/00007435-200002000-00011

    authors: Hammett TM,Mason TH,Joanis CL,Foster SE,Harmon P,Robles RR,Finlinson HA,Feudo R,Vining-Bethea S,Jeter G,Mayer KH,Doherty-Iddings P,Seage GR 3rd

    更新日期:2000-02-01 00:00:00

  • Male circumcision and women's risk of incident chlamydial, gonococcal, and trichomonal infections.

    abstract:BACKGROUND:Male circumcision (MC) decreases the risk of human immunodeficiency virus (HIV) acquisition in men. We explored associations between MC of the primary sex partner and women's risk of acquisition of chlamydial (Ct), gonococcal (GC), or trichomonal (Tv) infections. METHODS:We analyzed data from a prospective ...

    journal_title:Sexually transmitted diseases

    pub_type: 杂志文章

    doi:10.1097/OLQ.0b013e31816b1fcc

    authors: Turner AN,Morrison CS,Padian NS,Kaufman JS,Behets FM,Salata RA,Mmiro FA,Chipato T,Celentano DD,Rugpao S,Miller WC

    更新日期:2008-07-01 00:00:00