Abstract：
:Retinoic acid (RA) affects the response of many cells to growth factors, including the bone morphogenetic proteins (BMPs). The BMPs are members of the TGF-beta, family of growth factors, originally identified by their bone-inducing activities. Their widespread expression suggests many roles other than that in osteogenesis. Because RA modulates the cell's response to growth factors, this may be a means by which the retinoids exert some of their known teratogenic effects. One such cellular response may be apoptosis. While apoptosis is required for normal development, the location and timing of its induction must be carefully controlled. Recently, several TGF-beta family members have been implicated in the induction of apoptosis in certain cell types. We show here, using P19 embryonal carcinoma cells, that the combination of RA and BMP2 or BMP4 synergistically induces apoptosis in 40% of the population within 24 hr. In contrast, RA alone induces apoptosis in only 10-15% of the population and each of the BMPs alone minimally induces apoptosis. Apoptosis depends on the dose of both the RA and the BMP as well as on new protein synthesis. Further, the induction of apoptosis prevents the formation of fully differentiated neurons and glial cells and instead leads to primarily smooth muscle cell differentiation. These results suggest that some of the malformations caused by retinoids may be due to the induction of inappropriate apoptosis in cells exposed to BMPs.
journal_name
Dev Bioljournal_title
Developmental biologyauthors
Glozak MA,Rogers MBdoi
10.1006/dbio.1996.0275subject
Has Abstractpub_date
1996-11-01 00:00:00pages
458-70issue
2eissn
0012-1606issn
1095-564Xpii
S0012-1606(96)90275-4journal_volume
179pub_type
杂志文章abstract：:We have investigated the accumulation and adenylation of the maternal mRNA during oogenesis in the oocytes of the marine worm Urechis caupo. The analysis, using in vitro translation and cDNA probes to assay for specific mRNAs, demonstrates that different maternal mRNAs accumulate with different patterns during oogenes...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(86)90347-7
更新日期：1986-09-01 00:00:00
abstract：:The formation of the spinal cord during early embryonic development in vertebrate embryos is a continuous process that begins at gastrulation and continues through to the completion of somitogenesis. Despite the conserved usage of patterning mechanisms and gene regulatory networks that act to generate specific spinal ...
journal_title：Developmental biology
pub_type： 杂志文章,评审
doi：10.1016/j.ydbio.2017.01.021
更新日期：2017-12-01 00:00:00
abstract：:The formation of an anterior-posterior (AP) gradient of microtubules in Drosophila oocytes is essential for specification of the AP axis. Proper microtubule organization in the oocyte requires the function of serine/threonine kinase Par-1. The N1S isoform of Par-1 is enriched at the posterior cortex of the oocyte from...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2008.12.031
更新日期：2009-03-15 00:00:00
abstract：:NK genes are related pan-metazoan homeobox genes. In the fruitfly, NK genes are clustered and involved in patterning various mesodermal derivatives during embryogenesis. It was therefore suggested that the NK cluster emerged in evolution as an ancestral mesodermal patterning cluster. To test this hypothesis, we cloned...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2008.02.013
更新日期：2008-05-15 00:00:00
abstract：:The thyroid gland synthesizes thyroxine (T4), which passes through the larval tadpole's circulatory system. The enzyme type II iodothyronine deiodinase (D2) converts thyroxine (T4) to the active hormone 3,5,3'-triiodothyronine (T3) in peripheral tissues. An early response to thyroid hormone (TH) in the Xenopus laevis ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2003.10.005
更新日期：2004-02-01 00:00:00
abstract：:Mammalian sperm-egg adhesion occurs when sperm bind to the zona pellucida of the egg. In this study with guinea pig gametes, we have asked if sperm can initiate binding to the zona before and after the acrosome reaction and if the sperm surface protein PH-20 is involved in the binding at these two stages. Sperm bindin...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(87)90191-6
更新日期：1987-06-01 00:00:00
abstract：:Planarians are well-known for their exceptional regenerative abilities. This investigation focuses on the involvement of a Y-box protein, defined by the presence of a cold-shock domain, in regeneration-specific processes. Previous studies have shown that developmentally expressed Y-box proteins bind to mRNA molecules ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1998.8996
更新日期：1998-09-15 00:00:00
abstract：:Vertebrate neural tube formation involves two distinct morphogenetic events--convergent extension (CE) driven by mediolateral cell intercalation, and bending of the neural plate driven largely by cellular apical constriction. However, the cellular and molecular biomechanics of these processes are not understood. Here,...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2008.12.009
更新日期：2009-03-15 00:00:00
abstract：:To determine whether spontaneous release of transmitter from the growth cones of neurons exhibits properties similar to the spontaneous release which occurs from the neurons at the neuromuscular junction, release of transmitter from the growth cones of Xenopus neurons in culture was monitored in salines containing var...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(86)90172-7
更新日期：1986-02-01 00:00:00
abstract：:During the process of gastrulation in the sea urchin embryo, the vegetal plate invaginates to form the archenteron, a long narrow tube that extends across the blastocoel. Rearrangement of cells within the archenteron is thought to be a key component of the process of archenteron elongation. While these cell rearrangem...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1994.1154
更新日期：1994-06-01 00:00:00
abstract：:In the head of an embryo, a layer of mesenchyme surrounds the brain underneath the surface ectoderm. This cranial mesenchyme gives rise to the meninges, the calvaria (top part of the skull), and the dermis of the scalp. Abnormal development of these structures, especially the meninges and the calvaria, is linked to si...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2019.07.015
更新日期：2019-11-15 00:00:00
abstract：:Canonical Wnt signalling is known to be involved in the regulation of differentiation and proliferation in the context of endodermal organogenesis. Wnt mediated beta-catenin activation is understood to be modulated by secreted Frizzled-related proteins, such as XsFRP5, which is dynamically expressed in the prospective...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2009.03.004
更新日期：2009-05-15 00:00:00
abstract：:To identify guanine nucleotide binding proteins (G-proteins) in sea urchin eggs and to investigate their role in signal transduction at fertilization, we used cholera toxin (CTX) and pertussis toxin (PTX), which catalyze the specific ADP-ribosylation of G-proteins. Cell surface complex, consisting of plasma membranes ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(87)90260-0
更新日期：1987-04-01 00:00:00
abstract：:Spermatogenesis is initiated by the interaction of germ cells and somatic cells in seminiferous tubules. We used cDNA microarrays and representational difference analysis to identify genes that are expressed in the testis of the jsd/jsd mutant mouse, which contains only type A spermatogonial germ cells and Sertoli cel...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2002.0671
更新日期：2002-06-15 00:00:00
abstract：:The clonal multipotential RCJ 3.1 cell line, which gives rise to myotubes, adipocytes, chondrocytes, and osteoblasts, contains different progenitor subpopulations. By limiting dilution analysis, of 296 single colonies identified, approximately 20% contained a single recognizable cell type, approximately 10% contained ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(90)90352-j
更新日期：1990-12-01 00:00:00
abstract：:Initial studies suggested that spatial organization of the putative polar body contractile ring was determined by the peripheral aster in Spisula [Biol. Bull. 205 (2003) 192]. Here we report detailed supporting observations, including testing of aster and ring function with inhibitors. The metaphase peripheral aster w...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2004.01.033
更新日期：2004-05-15 00:00:00
abstract：:Vertebrate pigment cells are derived from neural crest, a tissue that also forms most of the peripheral nervous system and a variety of ectomesenchymal cell types. Formation of pigment cells from multipotential neural crest cells involves a number of common developmental processes. Pigment cells must be specified; the...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2000.9840
更新日期：2000-09-15 00:00:00
abstract：:Proper development of a multicellular organism relies on well-coordinated regulation of cell fate specification, cell proliferation and cell differentiation. The C. elegans postembryonic mesoderm provides a useful system for uncovering factors involved in these processes and for further dissecting their regulatory rel...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2017.06.011
更新日期：2017-09-01 00:00:00
abstract：:A glycoprotein from the liver, which shares epitopes with chorion (egg envelope or zona pellucida) glycoproteins, is present only in the spawning female fish, Oryzias latipes, under natural conditions. This spawning female-specific (SF) substance is distinct from vitellogenin but closely resembles a major glycoprotein...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(89)90301-1
更新日期：1989-05-01 00:00:00
abstract：:HP1 proteins are essential components of heterochromatin and contribute to the transcriptional repression of euchromatic genes. Although most species contain more than one HP1 family member which differ in their chromosomal distribution, it is not known to what extent the activity of these different family members is ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2006.06.039
更新日期：2006-10-01 00:00:00
abstract：:The lizards are evolutionarily the closest vertebrates to humans that demonstrate the ability to regenerate entire appendages containing cartilage, muscle, skin, and nervous tissue. We previously isolated PAX7-positive cells from muscle of the green anole lizard, Anolis carolinensis, that can differentiate into multin...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2017.08.037
更新日期：2018-01-15 00:00:00
abstract：:The epithelial-mesenchymal transition of cardiac endothelium is a critical developmental event in the formation of valvular and septal anlagen. We have demonstrated previously that this event can be mimicked in culture by treating atrioventricular canal (AV) endothelium with EDTA-soluble proteins extracted from embryo...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(91)90121-i
更新日期：1991-06-01 00:00:00
abstract：:Following their birth in olfactory placode, luteinizing hormone-releasing hormone (LHRH)-containing neurons migrate across the developing cribriform plate and form a dispersed population in the mammalian basal forebrain. The present study reveals the colocalization of unique glycoconjugate antigens (detected with mono...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1993.1045
更新日期：1993-02-01 00:00:00
abstract：:Several genomic clones were isolated from a Drosophila library screened with cDNA prepared from abundant adult female mRNA. Cytoplasmic dot hybridizations have shown that the genes in all of these clones are expressed only in posteclosion (stages 8-14) follicles. One set of overlapping clones (lambda 20, lambda 28, an...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(84)90271-9
更新日期：1984-09-01 00:00:00
abstract：:The contribution of cranial neural crest cells to the development and patterning of cranial muscles in amphibians was investigated in the phylogenetically basal and morphologically generalized frog, Bombina orientalis. Experimental methods included fluorescent marking of premigratory cranial neural crest and extirpati...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2001.0377
更新日期：2001-09-15 00:00:00
abstract：:Adherent cultures of E10.5 rat neuroepithelial cells (NEP cells) from the caudal neural tube require FGF (fibroblast growth factor) and CEE (chick embryo extract) to proliferate and maintain an undifferentiated phenotype in culture. Epidermal growth factor (EGF) does not support E10.5 NEP cells in adherent culture and...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1997.8592
更新日期：1997-06-15 00:00:00
abstract：:Fertilized eggs of the leech Helobdella triserialis undergo a cytoplasmic reorganization which generates domains of nonyolky cytoplasm, called teloplasm, at the animal and vegetal poles. The segregation of teloplasm to one cell of the eight-cell embryo is responsible for a unique developmental fate of that cell, i.e.,...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(89)90181-4
更新日期：1989-10-01 00:00:00
abstract：:Paracrine signaling mediated by FGF-10 and the FGF-R2IIIb receptor is required for formation of the lung. To determine the temporal requirements for FGF signaling during pulmonary morphogenesis, Sprouty-4 (Spry-4), an intracellular FGF receptor antagonist, was expressed in epithelial cells of the fetal lung under cont...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/s0012-1606(03)00106-4
更新日期：2003-06-01 00:00:00
abstract：:Effective transgenesis methods have been successfully employed in many organisms including zebrafish. However, accurate spatiotemporal control of transgene expression is still difficult to achieve. Here we describe a system for chemical-inducible gene expression and demonstrate its feasibility for generating transgeni...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2008.04.042
更新日期：2008-08-01 00:00:00
abstract：:We have examined the interfaces between protein and lipid sulfation, the response of progenitor cells to specific growth factors, and developmental lineage progression. The experimental system used is the oligodendrocyte (OL), the cell responsible for myelination of axons in the central nervous system. The ability to ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1994.1105
更新日期：1994-04-01 00:00:00
