Detection of malignant effusions: comparison of a telomerase assay and cytologic examination.

Abstract:

:Telomerase is inactive in most somatic cells, but has been found to be reactivated in a majority of cancers. Our principal goal was to test whether the presence of telomerase activity concurred with positive cytology, and was thus of potential use in detecting cancer cells in effusions. The telomeric repeat amplification protocol (TRAP) assay and cytological examination were performed in a blinded fashion on 91 unselected effusions, for which laboratory processing was done according to standard procedures. In our series, 30% (27/91) of samples were found to be malignant by cytology. Of these, 19 (70%) were also positive in the TRAP assay. Of the 8 telomerase-negative cytology-positive samples, RNA integrity was generally poor, indicating suboptimal sample conservation for molecular analysis. Negative cytology in the presence of telomerase activity was observed in 17 effusions. Of these, 11 were from patients with advanced cancer, and thus a diagnosis of malignant effusion should be suspected. The TRAP assay for telomerase activity holds promise in the analysis of effusions, but its routine use as an adjunct to cytology awaits further confirmation of its positive predictive value.

journal_name

Diagn Cytopathol

journal_title

Diagnostic cytopathology

authors

Braunschweig R,Yan P,Guilleret I,Delacretaz F,Bosman FT,Mihaescu A,Benhattar J

doi

10.1002/1097-0339(200103)24:3<174::aid-dc1036>3.0.

subject

Has Abstract

pub_date

2001-03-01 00:00:00

pages

174-80

issue

3

eissn

8755-1039

issn

1097-0339

pii

10.1002/1097-0339(200103)24:3<174::AID-DC1036>3.0.

journal_volume

24

pub_type

杂志文章
  • Cytologic diagnosis of vulvar Paget's disease by means of brushing smear: report of a case.

    abstract::Paget's disease of the vulva is a rare entity; since it may show the same clinical symptoms as benign chronic vulvitis, it may result in a delayed diagnosis. A 63-year-old woman was found to have an eczematoid pink to red lesion located in the vulva. Scraping cytology of the vulva showed isolated malignant cells of Pa...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.21160

    authors: Funamoto Y,Haba R,Kobayashi TK,Kushida Y,Kouno K,Matsunaga T,Shiota A,Hata T

    更新日期:2010-02-01 00:00:00

  • Epidermal growth factor receptor and Kras gene expression: reliability of mutational analysis on cytological samples.

    abstract::Epidermal growth factor receptor (EGFR) and Kras gene mutations are crucial for discriminating patients responsive to anti-EGFR drugs in non-small cell lung cancer (NSCLC) and colorectal cancer (CRC), respectively. The majority of NSCLCs come to clinical attention at an advanced stage when surgery is no longer recomme...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.22905

    authors: Bozzetti C,Negri FV,Azzoni C,Naldi N,Nizzoli R,Bortesi B,Zobbi V,Bottarelli L,Tiseo M,Silini EM,Ardizzoni A

    更新日期:2013-07-01 00:00:00

  • Cytologic features of papillary tumor of the pineal region: A case report showing tigroid background.

    abstract::Papillary tumor of the pineal region (PTPR) is a rare neuroepithelial tumor of the pineal region of adults. We herein describe on a 34-year-old female the cytologic features of a PTPR studied using Diff-Quik stain. The patient presented hydrocephalia secondary to a pineal tumor. During surgery an intraoperative pathol...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.23560

    authors: Jiménez-Heffernan JA,Bárcena C,Gordillo C,Cañizal JM

    更新日期:2016-12-01 00:00:00

  • Cytologic diagnosis of salivary duct carcinoma: a review of seven cases.

    abstract::The cytomorphologic findings in fine-needle aspirates from 7 cases of salivary duct carcinoma (SDC) are reviewed and correlated with the histologic features. Malignant cytologic characteristics are clear in this tumor, and no false-negative results were obtained. But the absence of cribriform or papillary groups sugge...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/(sici)1097-0339(199808)19:2<120::aid-dc11>

    authors: García-Bonafé M,Catala I,Tarragona J,Tallada N

    更新日期:1998-08-01 00:00:00

  • The role of routine outpatient cytological screening for early detection of carcinoma of the cervix in India.

    abstract::Cytological evaluation of cervical smears has been carried out in 4,338 women attending the Gynecology and Obstetrics Department and Family Planning Clinic of Queen Mary's Hospital, Lucknow, India, since April 1971. The incidence of cervical dysplasia was found to be 4.6% (205 out of 4,338) in the series, while malign...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.2840030107

    authors: Engineer AD,Misra JS

    更新日期:1987-03-01 00:00:00

  • Fine-needle aspiration cytology of non-small cell lung carcinoma: A paradigm shift.

    abstract::Lung carcinoma is one of the commonest causes of cancer related death. Fine-needle aspiration cytology (FNAC) is a well-established technique in the diagnosis of various malignant tumors. FNAC is now an important technique in classifying lung carcinomas and also detecting salient mutational changes in lung carcinomas....

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章,评审

    doi:10.1002/dc.24089

    authors: Dey P,Ghosh RK

    更新日期:2019-04-01 00:00:00

  • Chordoma: cytologic and immunocytochemical study of four cases.

    abstract::We present the cytologic features and the immunocytochemical profile of four cases of chordoma on fine-needle aspiration biopsies. The physaliferous cells in signet-ring, pearl-like formations and the trabecular arrangement with rounded contours are distinctive. Other cell types and cellular arrangements are also desc...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.2840020112

    authors: Kontozoglou T,Qizilbash AH,Sianos J,Stead R

    更新日期:1986-01-01 00:00:00

  • Bilateral primary lymphoma of the breast: a case report initially diagnosed by FNAC.

    abstract::Primary lymphoma of the breast (PLB) is a rare disease, representing 0.04-0.5% of all malignant breast neoplasms. We present a patient with bilateral breast involvement by a high-grade diffuse large B-cell lymphoma, which was diagnosed initially by fine-needle aspiration cytology (FNAC). Mammography revealed a diffuse...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.20184

    authors: Maounis N,Ellina E,Papadaki T,Ioannides G,Kiale K,Finokaliotis N,Blana AK

    更新日期:2005-02-01 00:00:00

  • Fine needle aspiration cytology in sclerosing stromal tumor of the ovary: a series of three cases.

    abstract::Sclerosing stromal tumor (SST) of the ovary is a distinct subtype of sex cord-stromal tumor, which is derived from the ovarian stroma and, in turn, from the sex cords of the embryonic gonads. It is a benign tumor, predominantly, unilateral in nature and mostly involves the right side of the ovary. Histopathological fe...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.21639

    authors: Banik T,Gupta N,Dey P,Rajwanshi A,Dhaliwal LK

    更新日期:2012-04-01 00:00:00

  • Fine-needle aspiration biopsy findings in marginal zone B cell lymphoma.

    abstract::Marginal zone B cell lymphomas (MZBCLs) represent a category of non-Hodgkin's lymphoma which may arise in a wide variety of extranodal organs where they are termed low grade B cell lymphomas of mucosa-associated lymphoid tissue (MALT). MZBCLs may involve primarily lymph nodes and or spleen where they are designated mo...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/(sici)1097-0339(199904)20:4<190::aid-dc2>3

    authors: Matsushima AY,Hamele-Bena D,Osborne BM

    更新日期:1999-04-01 00:00:00

  • Epithelioid variant of gastrointestinal stromal tumor: Diagnosis by fine-needle aspiration.

    abstract::Epithelioid gastrointestinal stromal tumors (GISTs) may cause significant diagnostic confusion on fine-needle aspiration (FNA) with carcinomas, neuroendocrine tumors, and melanoma, particularly when metastatic. This study characterizes the cytologic features of nine cases of epithelioid GISTs that were obtained by com...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.10293

    authors: Dong Q,McKee G,Pitman M,Geisinger K,Tambouret R

    更新日期:2003-08-01 00:00:00

  • Adenomyoepithelioma of the breast: fine-needle aspiration biopsy and histologic findings.

    abstract::Adenomyoepithelioma is an uncommon tumor of the breast which clinically presents as a discrete nodule and histologically is composed of lumenal spaces lined by a mixture of epithelial and myoepithelial cells. It has a spectrum of histologic appearances which are gradually gaining wider recognition. There are, however,...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.2840120311

    authors: Niemann TH,Benda JA,Cohen MB

    更新日期:1995-05-01 00:00:00

  • Synovial sarcoma metastatic to the parotid gland: A possible pitfall in salivary gland fine needle aspiration cytology. Report of a case with immunocytochemical and fluorescence in situ hybridization findings.

    abstract::We report one case of metastatic synovial sarcoma (SS) to the parotid gland in a 93-year-old male. The patient had undergone upper left pulmonary lobectomy with mediastinal lymphadenectomy for SS of the lung 5 years before. The cytopathologic presentation and the immunocytochemical findings on the FNA sample were sugg...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.23725

    authors: Fulciniti F,Barizzi J,Martin V,Tarallo V,Ermanni S

    更新日期:2017-09-01 00:00:00

  • Bladder-washing cytology of metastatic ovarian granulosa cell tumor.

    abstract::An 86-year-old Caucasian female presented with two weeks history of discomfort discharging urine, occasional hematuria, and suprapubic pain. The patient had a history of left salpingo-oophorectomy for an ovarian tumor, performed four years earlier. Ultrasound showed a solid mass surrounding the orifice of the left ure...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.10095

    authors: Vodovnik A

    更新日期:2002-06-01 00:00:00

  • Cytological investigation of a first case of basal cell adenocarcinoma arising in the minor salivary glands. Case report and review of the literature.

    abstract::Basal cell adenocarcinoma (BCAC) of salivary glands is uncommon low-grade malignancy. Only 19 cases of preoperative cytology were reported up to date. We present here a new case of BCAC arising in the minor salivary gland. Tumor was composed of clustered or isolated roundish, dark cells with small and regular nuclei. ...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章,评审

    doi:10.1002/dc.23919

    authors: Mokhniuk K,Lesnik M,Klijanienko J

    更新日期:2018-08-01 00:00:00

  • Chondroid syringoma diagnosed by fine needle aspiration cytology.

    abstract::Chondroid syringoma is a rare benign skin adnexal tumor of eccrine/apocrine origin affecting commonly the head and neck region. It used to be previously called as mixed tumor of skin because of the presence of both the epithelial and mesenchymal components. There are only few case reports describing the fine needle as...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.21159

    authors: Kumar B

    更新日期:2010-01-01 00:00:00

  • Evaluation of NMP179 for the detection of squamous intraepithelial neoplasia in ThinPrep cervical slides using a combination of double immunostaining and morphometric methods of analysis.

    abstract::This study investigates the potential value of the nuclear matrix protein NMP179 as a marker of abnormal squamous cells in ThinPrep slides. Forty-six cervical scrapes were collected as cell suspensions and ThinPrep slides were prepared. They were double-immunostained for NMP179 and Cytokeratin 18 (CK18), an endocervic...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.20061

    authors: Al-Awadhi R,Van Noorden S,Coleman DV

    更新日期:2004-09-01 00:00:00

  • Diagnostic role of fine-needle aspiration of pancreatic allograft to detect rejection.

    abstract::The purpose of this study was to assess whether the same principles to evaluate renal transplant by fine-needle aspiration (FNA) for rejection could be applied to pancreatic allograft. Between 1996-1998, 25 ultrasound-guided FNAs on 13 patients with pancreatic allograft were performed and ThinPrep made. The percentage...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.10179

    authors: Sariya D,Kluskens L,Assad L,Treaba D,Reddy V,Gattuso P

    更新日期:2002-11-01 00:00:00

  • Anaplastic spindle-cell squamous carcinoma arising in association with tall-cell papillary cancer of the thyroid: A potential pitfall

    abstract::The following is a correction to the original article that appeared in Diagnostic Cytopathology 1999;21:413-418. Owing to a publisher's error, Figure 4C on p. 415 was incorrectly reproduced. The correct illustrations and captions for this page are given here. ...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/(sici)1097-0339(200002)22:2<136::aid-dc19>

    authors: Saunders CA,Nayar R

    更新日期:2000-02-01 00:00:00

  • Primary hydatid cyst of the supraspinatus muscle: complete removal of the germinal layer and cytodiagnosis by fine-needle aspiration.

    abstract::Primary hydatid disease of the skeletal muscle without systemic involvement is rare. The purpose of this report is to document the novel clinical presentation and the interesting facets of fine-needle aspiration in a case of hydatid disease. It was a case of primary hydatid cyst of the left supraspinatus muscle in an ...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.22925

    authors: Das DK,El-Sharawy M,Ayyash EH,Al-Enezi NA,Iqbal JR,Madda JP

    更新日期:2014-03-01 00:00:00

  • Diagnosis and differential diagnosis of small-cell lesions of the liver.

    abstract::The objective of this study was to evaluate the cytologic spectrum of small cell lesions of liver as diagnosed by fine-needle aspiration (FNA). Of 304 FNAs of liver that were performed in our institution between 1990 and 1994, 29 were classified as small-cell lesions. The final diagnoses were as follows: small-cell un...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/(sici)1097-0339(199807)19:1<29::aid-dc6>3.

    authors: Pisharodi LR,Bedrossian C

    更新日期:1998-07-01 00:00:00

  • Predictors of human papilloma virus in patients with keratinization.

    abstract::Given the prevalence of human papilloma virus (HPV) infection, an attempt was made to determine whether certain factors such as keratinization and/or squamous atypia are associated with its development. Review of our gynecologic cytology files from 1989 yielded 1,615 specimens showing parakeratosis and/or hyperkeratos...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.2840120107

    authors: Hudock J,Hanau CA,Hawthorne C,Jordan AG

    更新日期:1995-02-01 00:00:00

  • Koilocytosis: correlations with high-risk HPV and its comparison on tissue sections and cytology, urothelial carcinoma.

    abstract::The aim of this study were (1) To correlate koilocytosis with high risk HPV(HrHPV) DNA in urinary bladder carcinoma and (2) To compare detection of koilocytosis on tissue sections and urine cytology. Biopsy and cytologic specimens from 33 patients of urinary bladder carcinoma were analyzed. HPV DNA was detected by PCR...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.20978

    authors: Aggarwal S,Arora VK,Gupta S,Singh N,Bhatia A

    更新日期:2009-03-01 00:00:00

  • Aspiration cytopathology of middle-ear neuroendocrine carcinoma.

    abstract::Primary neuroendocrine carcinoma (carcinoid tumor) is among the rarest of middle-ear (ME) neoplasms, with only a rare case of metastatic disease having been reported. Fine-needle aspiration biopsy (FNAB) was performed in a 51-yr-old male with a two-decade history of multiple local recurrences from a right middle-ear n...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.2031

    authors: Menezes G,Wakely P Jr

    更新日期:2001-09-01 00:00:00

  • Case report: different metastatic components from anaplastic thyroid carcinoma in mediastinal and neck lymph nodes simultaneously diagnosed by FNA.

    abstract::Anaplastic thyroid carcinoma (ATC) is a rare but aggressive form of undifferentiated thyroid carcinoma which arises from previously well-differentiated thyroid carcinomas, such as papillary carcinoma or follicular carcinoma. We report on an interesting case of ATC found in an enlarging neck mass with metastatic papill...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.23016

    authors: Costaldi ME,Zhou Z

    更新日期:2014-08-01 00:00:00

  • Barr body in fine needle aspiration cytology of ovarian malignancies.

    abstract::The Barr body is the inactive X chromosome in a female somatic cell. It is readily identified as plano-convex structure of 2-3 micron in diameter on the periphery of the nuclear membrane. The aim of this study is to evaluate the significance of Barr body count in malignant ovarian tumors on fine needle aspiration cyto...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.21688

    authors: Agrawal P,Dey P

    更新日期:2012-11-01 00:00:00

  • Evaluation of flow cytometric immunophenotyping and DNA analysis for detection of malignant cells in serosal cavity fluids.

    abstract::The serosal cavities are frequent sites of tumor metastasis. The distinction between carcinoma cells, inflammatory cells, and reactive or malignant mesothelial cells can be difficult in cytology. Multicolor flow cytometry (FCM) provides the opportunity to evaluate multiple antigens simultaneously, making it possible t...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.21047

    authors: Sayed DM,el-Attar MM,Hussein AA

    更新日期:2009-07-01 00:00:00

  • Prevalence of epithelial abnormalities and high-risk human papilloma virus in cervicovaginal Pap smears of population subgroups as a guide toward evidence-based best practice.

    abstract:BACKGROUND:The 2012 American Society for Colposcopy and Cervical Pathology Consensus Guidelines provide information for managing abnormal cervical cancer screening tests and cancer precursors. According to these guidelines for Pap smear diagnosis of Atypical squamous cells of undetermined significance, reflex high risk...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.24158

    authors: Siddiqi A,Webb F,Smotherman C,Shuja S,Mubeen A,Lai K

    更新日期:2019-07-01 00:00:00

  • Nodular sclerosing classical Hodgkin lymphoma masquerading as acute suppurative-necrotizing lymphadenitis.

    abstract::The diagnosis of nodular sclerosing classical Hodgkin lymphoma (NSCHL) by fine-needle aspiration (FNA) biopsy has historically been a diagnostic challenge due to the usual paucicellularity of the specimen. This case report, and other previously published reports, suggests that there is another facet to the potentially...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.21829

    authors: Florentine BD,Cohen AN

    更新日期:2014-03-01 00:00:00

  • Fine-needle aspiration cytology of adenoid (acantholytic) squamous-cell carcinoma.

    abstract::Adenoid squamous-cell carcinoma (ASCCa) is an uncommon cancer which occurs most frequently in the skin of the head and neck region of elderly, sun-exposed individuals. Histologically, ASCCa is characterized by gland-like, "adenoid" cell groups with a central, detached acantholytic cellular component and an intact peri...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.2840120218

    authors: Dodd LG

    更新日期:1995-03-01 00:00:00