Abstract：
:We previously reported that PC12h cells are killed by a high oxygen atmosphere. In this study, we further characterized this oxygen-induced cell death and found apoptotic features, as follows. Firstly, chromatin condensation was observed in cells cultured in a 50% O2 atmosphere. Secondly, cycloheximide and cordycepin, protein and RNA synthesis inhibitors, respectively, prevented the oxygen-induced cell death in PC12h cells, suggesting that it is mediated by an intracellular death program. Thirdly, NGF, CPT-cAMP and depolarization by high potassium medium also effectively inhibited this apoptotic cell death in PC12h cells. The effect of high K+ is thought to be mediated by the influx of Ca2+ into cells through voltage-dependent Ca2+ channels, because nifedipine, an L-type Ca2+ channel blocker, inhibited the effect of high K+. In addition, since the oxygen-induced apoptosis was blocked by the antioxidant vitamin E, this oxygen toxicity is suggested to be mediated by reactive oxygen species. To further characterize this oxygen-induced apoptosis at the molecular level, we used PC12 cells overexpressing the proto-oncogene bcl-2. Although a large number of PC12 cells transfected with the control vector died in a 50% O2 atmosphere within 6 days, bcl-2-transfected PC12 cells survived and proliferated. These findings suggested that our system using PC12 cells will be a useful model with which to analyze the molecular mechanisms of apoptosis induced by oxidative stress in neuronal cells.
journal_name
Brain Resjournal_title
Brain researchauthors
Kubo T,Enokido Y,Yamada M,Oka T,Uchiyama Y,Hatanaka Hdoi
10.1016/0006-8993(96)00542-2subject
Has Abstractpub_date
1996-09-16 00:00:00pages
175-83issue
2eissn
0006-8993issn
1872-6240pii
0006-8993(96)00542-2journal_volume
733pub_type
杂志文章相关文献
BRAIN RESEARCH文献大全abstract：:Changes in the expression and activation of protein kinase C (PKC) have been implicated in the pathogenesis of diabetic neuropathy. Recent studies in liver, retina, and cardiovascular tissues from experimentally diabetic rats have demonstrated that diabetes has a selective effect on the expression and subcellular dist...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(97)00062-0
更新日期：1997-04-18 00:00:00
abstract：:Electrical stimulation of the chorda tympani nerve (CT; innervating taste buds on the rostral tongue) is known to initiate recurrent inhibition in cells in the nucleus of the solitary tract (NTS, the first central relay in the gustatory system). Here, we explored the relationship between inhibitory circuits and the br...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2009.03.069
更新日期：2009-06-12 00:00:00
abstract：:The effects of hyperbaric oxygen have been studied on synaptic transmission in the hippocampal brain slice. Oxygen enhances both the prevolley and postsynaptic potential of extracellularly recorded responses following focal stimulation of afferent fiber bundles, but synaptic efficiency is reduced. It is suggested that...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(83)90775-8
更新日期：1983-01-31 00:00:00
abstract：:Drugs thought to inhibit the actions of protein kinase C (PKC) and cAMP dependent protein kinase (A-kinase) were infused intrathecally into the subarachnoid space of the lumbar region of the spinal cord, and the effects on acoustic startle were measured. Previous work has shown that intrathecal infusion of drugs thoug...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(90)90864-8
更新日期：1990-08-20 00:00:00
abstract：:While exposure of cultured spinal neurons to mild hypothermia provides some protection from physical trauma (dendrotomy), profound cooling (< 17 degrees C) causes unrelated neuronal injury and death, which can be prevented by treatment with NMDA receptor antagonists. To investigate the mechanism of hypothermic neurona...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(95)00726-7
更新日期：1995-09-18 00:00:00
abstract：:Anterograde and retrograde axonal tracing methods have been combined with transection of the stria terminalis to investigate the centrifugal afferent connections of the accessory olfactory bulb in the mouse. Injection of tritiated proline into the postero-medial cortical amygdaloid nucleus (C3) gives rise to anterogra...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(82)90803-4
更新日期：1982-08-12 00:00:00
abstract：:Intracellular recordings were made from Renshaw cells in the cat. Recurrent excitatory postsynaptic potentials (EPSPs) were elicited by antidromic stimulation of the ventral roots. These EPSPs had a simple monophasic time course without a spike-like peak reported by previous authors. No hyperpolarization could be dete...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(81)90818-0
更新日期：1981-10-26 00:00:00
abstract：:The rate and pattern of seizure development provoked by repeated electrical stimulation of the amygdala (kindling) was assessed in rats that had been pretreated with intracerebral injections of the selective catecholaminergic neurotoxin 6-hydroxy-dopamine. Rats with selective depletion of forebrain noradrenaline displ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(80)90289-9
更新日期：1980-05-26 00:00:00
abstract：:Saccadic adaptation maintains saccade accuracy and has been studied with targeting saccades, i.e. saccades that bring the gaze to a target, with the classical intra-saccadic step procedure in which the target systematically jumps to a new position during saccade execution. Post-saccadic visual feedback about the error...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2011.07.029
更新日期：2011-09-30 00:00:00
abstract：:These experiments used in vivo microdialysis and high-performance liquid chromatography to examine, in rats, norepinephrine (NE) release in the amygdala induced by footshock and systemic administration of drugs affecting the opioid peptidergic system. A microdialysis probe was inserted into a previously implanted guid...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(98)00795-1
更新日期：1998-10-19 00:00:00
abstract：:Fascia dentata tissue from embryonic mice was grafted to the hippocampal region of newborn Kyoto rats. After 1-7 months the recipients received lesions of the entorhinal cortex on the side of transplantation. Three days later their brains were processed for electron microscopy. The xenografts were identified by their ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(87)91036-5
更新日期：1987-06-16 00:00:00
abstract：:The ability of embryonic tissue to survive cross-transplantation between histologically incompatible rat strains was examined by transplanting septal neurons from Sprague Dawley fetuses to adult Wistar rats (Ag-B6 to Ag-B2 histocompatibility haplotype). Transplants were found to survive without rejection over a period...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(83)91028-4
更新日期：1983-03-07 00:00:00
abstract：:Chronic administration of caffeine (s.c. for a period of 14 days in escalating doses of 10-70 mg/kg) increased the sensitivity of rat cerebral cortical neurons to the inhibitory action of microiontophoretically applied adenosine. The sensitivity of spontaneously firing rat cerebral cortical neurons in caffeine-treated...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(90)91722-s
更新日期：1990-06-18 00:00:00
abstract：:Lifelong bilingualism is associated with the delayed diagnosis of dementia, suggesting bilingual experience is relevant to brain health in aging. While the effects of bilingualism on cognitive functions across the lifespan are well documented, less is known about the neural substrates underlying differential behaviour...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2015.02.034
更新日期：2015-07-01 00:00:00
abstract：:Various chemical and physical lesions were employed to elucidate the cellular localization of cholecystokinin (CCK) receptors in the rat caudate nucleus. A wide knife cut severing all caudal afferents reduced caudate CCK receptors by approximately 25%, indicating that some receptors lie on axons and (or) terminals of ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(81)90284-5
更新日期：1981-08-24 00:00:00
abstract：:Insulin has emerged as an important neuropeptide. Central actions of insulin appear to oppose those in the periphery. Insulin is transported across the blood-brain barrier (BBB) by a saturable transport system. The permeability of the BBB to insulin is altered by various events, but no studies exist that have examined...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(00)03247-9
更新日期：2001-03-30 00:00:00
abstract：:The compound m-chlorophenylpiperazine (m-CPP) is used clinically to manipulate serotonergic function, though its precise mechanisms of actions are not well understood. m-CPP alters synaptic transmission and neuronal function in vertebrates by non-selective agonistic actions on 5-HT(1) and 5-HT(2) receptors. In this st...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(03)02271-6
更新日期：2003-04-18 00:00:00
abstract：:Glutamate is the main excitatory neurotransmitter in the central nervous system. This amino acid mediates learning and memory processes acting through ionotropic and metabotropic receptor binding. Metabotropic glutamate receptors (mGluRs) are G protein-coupled receptors that stimulate phospholipase C (PLC) or inhibit ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2007.01.010
更新日期：2007-06-11 00:00:00
abstract：:Dynorphin and other kappa opioid agonists are thought to elicit aversive actions and changes in motor activity through direct or indirect modulation of dopamine neurons in ventral tegmental area (VTA) and substantia nigra (SN), respectively. We comparatively examined the immunoperoxidase localization of anti-dynorphin...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(93)90693-h
更新日期：1993-02-05 00:00:00
abstract：:Lithium is detected in the mouse brain with the aid of a (n,alpha) nuclear reaction. The resolving power is of a few micron. The detection efficiency is estimated in a first series of experiments with brain sections of progressively increasing thickness. This allows us to make the microlocation of lithium in the brain...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(80)90239-5
更新日期：1980-10-13 00:00:00
abstract：:The role of histamine in mediating restraint stress-induced alterations in dopaminergic neuronal activity and alpha-melanocyte-stimulating hormone (alpha MSH) secretion was evaluated in male rats. Dopaminergic neuronal activity was estimated by measuring concentrations of the dopamine metabolite 3,4-dihydroxyphenylace...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(94)90399-9
更新日期：1994-08-08 00:00:00
abstract：:This report deals with a comparison of total and regional cerebral blood flow with red cell velocity in the microcirculation of the ecto- and suprasylvian areas of the cerebral cortex of the cat and their response to changes in systemic blood pressure. Total and regional blood flows were measured during a steady-state...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(84)90912-0
更新日期：1984-08-06 00:00:00
abstract：:The cloned 5-HT3 receptor from NCB-20 neuroblastoma cells was expressed in Xenopus oocytes. In these oocytes, 5-HT, the selective 5-HT3 receptor agonists, 2-methyl-5-HT and m-chlorophenylbiguanide activated an inward current which was sensitive to the specific 5-HT3 receptor antagonist LY278584. Cocaine (0.1 to 10 mic...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(94)01316-a
更新日期：1995-03-06 00:00:00
abstract：:Cortical sensory neurons synchronize their activity at multiple frequency bands after an external stimulation. In the somatosensory cortical areas, previous reports describe more discrete and somatotopically specific neural synchronization at the gamma band. Therefore, an efficient gamma synchronization of the neurons...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(03)03171-8
更新日期：2003-10-03 00:00:00
abstract：:Mild footshock stress results in the metabolic activation of the prefrontal cortical dopamine (DA) innervation, but does not augment DA utilization in mesolimbic areas (such as the nucleus accumbens septi, NAS) or the striatum. However, increases in either the intensity or duration of footshock stress increase DA util...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(90)91557-w
更新日期：1990-06-25 00:00:00
abstract：:The effects of sex steroids on immunoreactive beta-endorphin (EP) release from the rat hypothalamus in vitro were examined using a rat hypothalamic perifusion system and an EP RIA. Testosterone (1-100 ng/ml) and estradiol (10-100 pg/ml) stimulated EP release in a dose-dependent manner. Dehydroepiandrosterone (DHEA, 1-...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(91)90221-g
更新日期：1991-07-05 00:00:00
abstract：:The importance of Na+/Ca2+ exchangers in the regulation of the physiological and pathological functions of the nervous system has been widely recognized. In this study, we used primary cultured E14.5 cortical neurons as a model system to study the possible roles of the reverse mode Na+/Ca2+ exchange activity in neurot...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2008.01.042
更新日期：2008-03-27 00:00:00
abstract：:The ability to detect moving objects is crucial for survival and is a basic capability in the animal kingdom. In the optic tectum, a major center of the visual pathway of birds, neurons have been found that seem to be perfectly suited to encode motion. These cells, located in the stratum griseum centrale, receive a di...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2016.01.022
更新日期：2016-03-15 00:00:00
abstract：:Prefrontal impairments have been hypothesized to be most strongly associated with the cognitive and emotional dysfunction in depression. Recently, white matter microstructural abnormalities in prefrontal lobe have been reported in elderly patients with major depressive disorder (MDD) using diffusion tensor imaging (DT...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2007.06.094
更新日期：2007-09-07 00:00:00
abstract：:Recordings of single unit activity before, during and after a 1-h continuous exposure were made from the mitral cell layer of the olfactory bulbs of awake rabbits. Thirteen complete 1-h exposures were performed using 8 neurons, while a further 6 neurons were lost before the end of the exposure. During the first few mi...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(82)90951-9
更新日期：1982-10-28 00:00:00
