Release of mast cell tryptase from human colorectal mucosa in inflammatory bowel disease.

Abstract:

BACKGROUND:Histologic detection of mast cells cannot adequately reflect their function and state of activation, since degranulated mast cells may escape from histologic assessment. To better define the role of mast cells in inflammatory bowel disease, the spontaneous secretion of mast cell tryptase, a highly mast cell specific protease, was measured from colorectal samples. METHODS:After detection of the initial basal tryptase release, gut mucosal samples were incubated in a modified Hanks/RPM1 medium using a mucosa oxygenation system. Spontaneous tryptase secretion from 153 viable samples of 22 controls, 30 patients with Crohn disease (CD) and 19 with ulcerative colitis (UC) was followed over 4 h. Tryptase was measured by radioimmunoassay. RESULTS:The rates of the initial basal tryptase release revealed that mast cell activation occurs during active inflammation in CD and UC. While the time course of tryptase release was similar in all three groups, spontaneous tryptase secretion (over 4 h) was found to be significantly enhanced and prolonged only in UC (P < 0.01 compared to controls), but not in CD. CONCLUSIONS:This study provides clear evidence from viable endoscopic colorectal samples that mast cell mediators were secreted during active inflammation in CD and UC. However, the extent of mast cell involvement and activation differs considerably between CD and UC. Significantly increased rates of tryptase secretion were found both in non-inflamed and inflamed tissue of UC, indicating that mast cell involvement is a typical feature of UC.

journal_name

Scand J Gastroenterol

authors

Raithel M,Winterkamp S,Pacurar A,Ulrich P,Hochberger J,Hahn EG

doi

10.1080/003655201750065933

subject

Has Abstract

pub_date

2001-02-01 00:00:00

pages

174-9

issue

2

eissn

0036-5521

issn

1502-7708

journal_volume

36

pub_type

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